Proteomic analysis of plasma proteins in Japanese semisuper centenarians

We performed proteomic analysis of plasma proteins in Japanese semisuper centenarians (SSCs) (> 105 years) and young controls (20-39 years), and found that 18 protein spots were altered in the plasma of SSCs. From peptide mass fingerprinting following in-gel digestion, it was demonstrated that paraoxonase/arylesterase 1 (PON 1) and apolipoprotein E were decreased, while haptoglobin beta-chain, alpha(1)-microglobulin, and clusterin precursor were increased in SSCs. Interestingly, proteins related to oxidative stress, PON1, haptoglobin, alpha(1)-microglobulin, and clusterin, were altered in SSCs. These results suggest that systemic redox regulation is important for the longevity of SSCs. Overall, proteomics analysis is a powerful technique to search for useful biomarkers for future studies in gerontology and to characterize the individual proteins associated with successful aging of SSCs. (c) 2010 Elsevier Inc. All rights reserved.