期刊
EXPERIMENTAL GERONTOLOGY
卷 43, 期 9, 页码 833-839出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exger.2008.06.001
关键词
telomere length; telomere centromere ratio; tissue Q-FISH; tongue; squamous mucosa; telomere reduction; stem cells
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [B17390108, 18659104, C17590325, C18590354]
- Grants-in-Aid for Scientific Research [18659104] Funding Source: KAKEN
We investigated the telomere lengths of individual cell types in lingual mucosa using an improved tissue quantitative fluorescence in situ hybridization (Q-FISH) method. Our tissue Q-FISH method compensates for partially cut nuclei in a tissue section by using the telomere:centromere ratio (TCR). We normalized our TCR measurements (NTCR) using a section from a block of cultured cells placed on the same slide, thus improving the accuracy and reproducibility of the results. Normal lingual mucosa was obtained from 21 autopsied individuals. Immunohistochemistry showed positivity mainly for p27, p63, and CK19 in basal cells, and for Ki-67 in parabasal cells. Q-FISH revealed that NTCR was significantly highest in basal cells and lowest in prickle cells, and also that telomere length regressed at a certain rate in each cell type, firstly. Significant correlations of NTCR among the three epithelia] cell types were demonstrated. The present findings appear to support the theory that stem cells exist in the basal layer of the lingual epithelium. The reduction of telomere length with age and in each cell layer is consistent with the telomere biology theory of cell proliferation and differentiation in oral mucosa. (C) 2008 Elsevier Inc. All rights reserved.
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