Article
Veterinary Sciences
Lianci Peng, Hongliang Tian, Yi Lu, Kaixiang Jia, Jinrong Ran, Qi Tao, Gang Li, Chao Wan, Chao Ye, Edwin J. A. Veldhuizen, Hongwei Chen, Rendong Fang
Summary: Chicken cathelicidin-2 (CATH-2) has potent antimicrobial and immunomodulatory activities and modulates NLRP3 inflammasome activation by inducing caspase-1 activation and oligomerization of apoptosis-associated speck-like protein containing a carboxy-terminal caspase recruitment domain (ASC). The activation is mediated by K+ efflux, NEK7 protein, and cathepsin B.
VETERINARY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Huan Liu, Dexi Liu
Summary: In this study, a novel SARS-CoV-2 viral vector was developed, allowing for investigation by the broader scientific community. The vector transduces target cells in an ACE2-dependent manner, and the incorporation of the VEEV self-amplification mechanism increases gene expression level and extends transgene expression time. Furthermore, the vector has significant applications in neutralizing antibody quantification and antiviral drug testing.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Bingting Ma, Cuiqing Huang, Jun Ma, Ye Xiang, Xinzheng Zhang
Summary: VEEV is a lethal RNA virus that utilizes LDLRAD3 as a receptor for host cell entry. The structure of LDLRAD3-D1 in complex with VEEV provides insights into their interactions, potentially guiding the development of therapeutic strategies.
Article
Biochemistry & Molecular Biology
Nergis Imprachim, Yuliana Yosaatmadja, Joseph A. Newman
Summary: NSP14 is a dual function enzyme, consisting of an N-terminal exonuclease domain (ExoN) and a C-terminal guanine-N7-methyltransferase (N7-MTase) domain. The interaction between NSP14 and NSP10 enhances the nuclease activity. Structural analysis reveals conformational changes in the NSP14 ExoN domain upon binding of NSP10, which explains the stimulation of nuclease activity. Additionally, structural insights into the viral mRNA capping process are gained through the structure of NSP14 in complex with cap analogue (7Me)GpppG.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Huiling Kang, Jingyuan Cong, Chenlong Wang, Wenxin Ji, Yuhui Xin, Ying Qian, Xuemei Li, Yutao Chen, Zihe Rao
Summary: The study presents the cryo-EM structure of the complex of JUNV L protein with Z protein, revealing insights into how Z protein regulates JUNV L during viral life cycle and RNA synthesis. This work sheds light on the mechanism of JUNV transcription, replication, and regulation, providing a basis for the design of antivirals against viral infections.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Zi Liang, Renyu Xue, Xing Zhang, Manman Cao, Sufei Sun, Yunshan Zhang, Min Zhu, Ziyao Zhang, Kun Dai, Jun Pan, Guangli Cao, Chonglong Wang, Xiaolong Hu, Chengliang Gong
Summary: In this study, the beta-Arrestin 2 (BmArrestin 2) gene from the silkworm was cloned and characterized. It was found that BmArrestin 2 is mainly distributed in the cytoplasm. Overexpression of BmArrestin 2 promoted the replication of Bombyx mori nucleopolyhedrovirus (BmNPV) and B. mori cytoplasmic polyhedrosis virus (BmCPV), while silencing the gene inhibited viral replication. Blocking endogenous BmArrestin 2 decreased the invasion of recombinant BmNPV and BmCPV. These findings suggest that BmArrestin 2 could be a potential target for antiviral strategies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Balaji Olety, Yoshiko Usami, Yuanfei Wu, Paul Peters, Heinrich Gottlinger, Stephen P. Goff
Summary: The HIV-1 Nef protein downregulates the viral receptor CD4 and the antiviral proteins SERINC3 and SERINC5. In the absence of SERINC3 and SERINC5, Nef is no longer required for viral infectivity and replication in Jurkat Tag T cells, but remains crucial for HIV-1 replication in MOLT-3 cells. The Nef-mediated enhancement of HIV-1 replication in MOLT-3 cells is independent of the Nef-interacting kinases LCK and PAK2.
Article
Biology
Xuanting Wang, Carolina Q. Sacramento, Steffen Jockusch, Otavio Augusto Chaves, Chuanjuan Tao, Natalia Fintelman-Rodrigues, Minchen Chien, Jairo R. Temerozo, Xiaoxu Li, Shiv Kumar, Wei Xie, Dinshaw J. Patel, Cindy Meyer, Aitor Garzia, Thomas Tuschl, Patricia T. Bozza, James J. Russo, Thiago Moreno L. Souza, Jingyue Ju
Summary: This paper discovers that hepatitis C virus inhibitors Pibrentasvir and Ombitasvir can inhibit the SARS-CoV-2 exonuclease and have therapeutic potential when combined with SARS-CoV-2 polymerase inhibitors in viral cell cultures. Combination drugs that inhibit both the SARS-CoV-2 polymerase and exonuclease are supported for effective COVID-19 treatment.
COMMUNICATIONS BIOLOGY
(2022)
Article
Immunology
Ines Alves, Manuel Machado Vicente, Joana Gaifem, Angela Fernandes, Ana Mendes Dias, Claudia Sousa Rodrigues, Jose Carlos Oliveira, Nair Seixas, Luis Malheiro, Miguel Araujo Abreu, Rui Sarmento e Castro, Salome Soares Pinho
Summary: The study found that COVID-19 infection leads to a glycosylation reprogramming of circulating lymphocytes at diagnosis, with asymptomatic patients showing a specific glycosignature on T cells and an increased expression of viral-sensing receptor DC-SIGN in monocytes. Higher levels of DC-SIGN in monocytes at diagnosis are associated with better COVID-19 prognosis, suggesting a potential novel glycan-based response in T cells that could confer protection against SARS-CoV-2 infection in asymptomatic patients.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Microbiology
Austin Royster, Songyang Ren, Yutian Ma, Melissa Pintado, Eunice Kahng, Sean Rowan, Sheema Mir, Mohammad Mir
Summary: A novel compound called K31 has been discovered to bind to the highly conserved N protein of SARS-CoV-2, inhibiting its replication. This finding suggests that targeting the viral nucleocapsid protein may be a potential approach for developing therapeutic drugs against coronaviruses.
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Biochemical Research Methods
Jun Zhang, Bing Chen
Summary: High-resolution structural information is crucial for the rapid development of vaccines and treatments against emerging human pathogens. Structural biology methods have been at the forefront of research on SARS-CoV-2 and will continue to be instrumental in combating future public health threats.
Article
Biochemistry & Molecular Biology
Andrew H. Mack, Georgina Menzies, Alex Southgate, D. Dafydd Jones, Thomas R. Connor
Summary: The RNA-dependent RNA polymerase of SARS-CoV-2 is prone to errors, but these errors can be corrected by the exonuclease proofreading mechanism. The interplay between replicase fidelity and environmental pressures affects virus mutagenesis and evolutionary trajectory. A single distal mutation in NSP14 has been shown to significantly impact proofreading, leading to increased mutation accumulation and elevated evolutionary rate. Understanding these changes is crucial for understanding the population-wide evolution of the virus.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Chemistry, Medicinal
Marta Gargantilla, Clara Frances, Anmol Adhav, Alicia Forcada-Nadal, Belen Martinez-Gualda, Olaia Marti-Mari, Maria Luisa Lopez-Redondo, Roberto Melero, Clara Marco-Marin, Nadine Gougeard, Carolina Espinosa, Antonio Rubio-del-Campo, Rafael Ruiz-Partida, Maria del Pilar Hernandez-Sierra, Laura Villamayor-Belinchon, Jeronimo Bravo, Jose-Luis Llacer, Alberto Marina, Vicente Rubio, Ana San-Felix, Ron Geller, Maria-Jesus Perez-Perez
Summary: A new class of SARS-CoV-2 entry inhibitors has been identified and characterized, which can bind to the virus protein (S) and its receptor, interfering with their interaction and effectively inhibiting viral entry and infection. This discovery holds significant potential for the prevention and treatment of COVID-19.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Fritz Obermeyer, Martin Jankowiak, Nikolaos Barkas, Stephen F. Schaffner, Jesse D. Pyle, Leonid Yurkovetskiy, Matteo Bosso, Daniel J. Park, Mehrtash Babadi, Bronwyn L. MacInnis, Jeremy Luban, Pardis C. Sabeti, Jacob E. Lemieux
Summary: This study developed a model called PyR0 for rapid detection and characterization of new SARS-CoV-2 lineages, and identified mutations relevant to fitness. By applying PyR0 to all publicly available SARS-CoV-2 genomes, the researchers discovered mutations that increase fitness and predicted the growth of new lineages based on their mutational profile. The model also prioritizes mutations of biological and public health concern for further study.
Article
Microbiology
Wakako Furuyama, Miako Sakaguchi, Kento Yamada, Asuka Nanbo
Summary: In this study, a novel GP monitoring system was developed to model the Ebola virus lifecycle under non-high biosafety level conditions. The system enables real-time visualization of the glycoprotein during replication, providing a useful tool for studying the molecular mechanisms of Ebola virus replication.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Kevin R. Mott, Hadi Maazi, Sariah J. Allen, Mandana Zandian, Harry Matundan, Yasamin N. Ghiasi, Behrooz G. Sharifi, David Underhill, Omid Akbari, Homayon Ghiasi
Article
Oncology
Giles W. Robinson, Brent A. Orr, Gang Wu, Sridharan Gururangan, Tong Lin, Ibrahim Qaddoumi, Roger J. Packer, Stewart Goldman, Michael D. Prados, Annick Desjardins, Murali Chintagumpala, Naoko Takebe, Sue C. Kaste, Michael Rusch, Sariah J. Allen, Arzu Onar-Thomas, Clinton F. Stewart, Maryam Fouladi, James M. Boyett, Richard J. Gilbertson, Tom Curran, David W. Ellison, Amar Gajjar
JOURNAL OF CLINICAL ONCOLOGY
(2015)
Article
Oncology
Melanie F. Weingart, Jacquelyn J. Roth, Marianne Hutt-Cabezas, Tracy M. Busse, Harpreet Kaur, Antoinette Price, Rachael Maynard, Jeffrey Rubens, Isabella Taylor, Xing-gang Mao, Jingying Xu, Yasumichi Kuwahara, Sariah J. Allen, Anat Erdreich-Epstein, Bernard E. Weissman, Brent A. Orr, Charles G. Eberhart, Jaclyn A. Biegel, Eric H. Raabe
Article
Biochemistry & Molecular Biology
Dominik Sturm, Brent A. Orr, Umut H. Toprak, Volker Hovestadt, David T. W. Jones, David Capper, Martin Sill, Ivo Buchhalter, Paul A. Northcott, Irina Leis, Marina Ryzhova, Christian Koelsche, Elke Pfaff, Sariah J. Allen, Gnanaprakash Balasubramanian, Barbara C. Worst, Kristian W. Pajtler, Sebastian Brabetz, Pascal D. Johann, Felix Sahm, Jueri Reimand, Alan Mackay, Diana M. Carvalho, Marc Remke, Joanna J. Phillips, Arie Perry, Cynthia Cowdrey, Rachid Drissi, Maryam Fouladi, Felice Giangaspero, Maria Lastowska, Wieslawa Grajkowska, Wolfram Scheurlen, Torsten Pietsch, Christian Hagel, Johannes Gojo, Daniela Loetsch, Walter Berger, Irene Slavc, Christine Haberler, Anne Jouvet, Stefan Holm, Silvia Hofer, Marco Prinz, Catherine Keohane, Iris Fried, Christian Mawrin, David Scheie, Bret C. Mobley, Matthew J. Schniederjan, Mariarita Santi, Anna M. Buccoliero, Sonika Dahiya, Christof M. Kramm, Andre O. von Bueren, Katja von Hoff, Stefan Rutkowski, Christel Herold-Mende, Michael C. Fruehwald, Till Milde, Martin Hasselblatt, Pieter Wesseling, Jochen Roessler, Ulrich Schueller, Martin Ebinger, Jens Schittenhelm, Stephan Frank, Rainer Grobholz, Istvan Vajtai, Volkmar Hans, Reinhard Schneppenheim, Karel Zitterbart, V. Peter Collins, Eleonora Aronica, Pascale Varlet, Stephanie Puget, Christelle Dufour, Jacques Grill, Dominique Figarella-Branger, Marietta Wolter, Martin U. Schuhmann, Tarek Shalaby, Michael Grotzer, Timothy van Meter, Camelia-Maria Monoranu, Joerg Felsberg, Guido Reifenberger, Matija Snuderl, Lynn Ann Forrester, Jan Koster, Rogier Versteeg, Richard Volckmann, Peter van Sluis, Stephan Wolf, Tom Mikkelsen, Amar Gajjar, Kenneth Aldape, Andrew S. Moore, Michael D. Taylor, Chris Jones, Nada Jabado, Matthias A. Karajannis, Roland Eils, Matthias Schlesner, Peter Lichter, Andreas von Deimling, Stefan M. Pfister, David W. Ellison, Andrey Korshunov, Marcel Kool
Article
Virology
Harry H. Matundan, Kevin R. Mott, Sariah J. Allen, Shaohui Wang, Catherine J. Bresee, Yasamin N. Ghiasi, Terrence Town, Steven L. Wechsler, Homayon Ghiasi
JOURNAL OF VIROLOGY
(2016)
Article
Pathology
Teresa Santiago, Michael R. Clay, Sariah J. Allen, Brent A. Orr
Article
Oncology
Jeffrey A. Rubens, Sabrina Z. Wang, Antoinette Price, Melanie F. Weingart, Sariah J. Allen, Brent A. Orr, Charles G. Eberhart, Eric H. Raabe
Correction
Pathology
Teresa Santiago, Michael R. Clay, Sariah J. Allen, Brent A. Orr
Article
Clinical Neurology
Fausto J. Rodriguez, Jacqueline A. Brosnan-Cashman, Sariah J. Allen, M. Adelita Vizcaino, Caterina Giannini, Sandra Camelo-Piragua, Milad Webb, Marcus Matsushita, Nitin Wadhwani, Abeer Tabbarah, Dima Hamideh, Liqun Jiang, Liam Chen, Leonidas D. Arvanitis, Hussein H. Alnajar, John R. Barber, Alicia Rodriguez-Velasco, Brent Orr, Christopher M. Heaphy
Article
Neurosciences
Norman L. Lehman, Aisulu Usubalieva, Tong Lin, Sariah J. Allen, Quynh T. Tran, Bret C. Mobley, Roger E. McLendon, Matthew J. Schniederjan, Maria-Magdalena Georgescu, Marta Couce, Mohanpal S. Dulai, Jack M. Raisanen, Mousa Al Abbadi, Cheryl A. Palmer, Eyas M. Hattab, Brent A. Orr
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2019)
Article
Oncology
Sabrina Z. Wang, Brad Poore, Jesse Alt, Antoinette Price, Sariah J. Allen, Allison R. Hanaford, Harpreet Kaur, Brent A. Orr, Barbara S. Slusher, Charles G. Eberhart, Eric H. Raabe, Jeffrey A. Rubens
CLINICAL CANCER RESEARCH
(2019)
Article
Clinical Neurology
Gourish Monday, Julieann C. Lee, Ajay Ravindranathan, Javier E. Villanueva-Meyer, Quynh T. Tran, Sariah J. Allen, Jairo Barreto, Rohit Gupta, Pamela Doo, Jessica Van Ziftle, Courtney Onodera, Patrick Devine, James P. Grenert, David Samuel, Rong Li, Laura K. Metrock, Lee-way Jin, Reuben Antony, Mouied Alashari, Samuel Cheshier, Nicholas S. Whipple, Carol Bruggers, Corey Raffel, Nalin Gupta, Cassie N. Kline, Alyssa Reddy, Anu Banerjee, Matthew D. Hall, Minesh P. Mehta, Ziad Khatib, Ossama M. Maher, Carole Brathwaite, Melike Pekmezci, Joanna J. Phillips, Andrew W. Bollen, Tarik Tihan, John T. Lucas, Alberto Broniscer, Mitchel S. Berger, Arie Perry, Brent A. Orr, David A. Solomon
ACTA NEUROPATHOLOGICA
(2020)
Article
Multidisciplinary Sciences
Tara T. Doucet-O'Hare, Brianna L. DiSanza, Catherine DeMarino, Abigail L. Atkinson, Jared S. Rosenblum, Lisa J. Henderson, Kory R. Johnson, Jeffrey Kowalak, Marta Garcia-Montojo, Sariah J. Allen, Brent A. Orr, Mariarita Santi, Tongguang Wang, Saeed Fathi, Myoung Hwa Lee, Kevon Sampson, Wenxue Li, Zhengping Zhuang, Avindra Nath
Summary: AT/RT is a rare pediatric CNS cancer often associated with SMARCB1 mutations. This study shows that SMARCB1 regulates HML-2 expression, which in turn affects cell proliferation and gene signaling pathways in AT/RT. The activation of HML-2, dependent on SMARCB1 and its interaction with MYC, may play a crucial role in the pluripotency and formation of these undifferentiated tumors.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Sasi Arunachalam, Karol Szlachta, Samuel W. Brady, Xiaotu Ma, Bensheng Ju, Bridget Shaner, Heather L. Mulder, John Easton, Benjamin J. Raphael, Matthew Myers, Christopher Tinkle, Sariah J. Allen, Brent A. Orr, Cynthia J. Wetmore, Suzanne J. Baker, Jinghui Zhang
Summary: This study investigates the tumor invasion patterns in diffuse midline gliomas with H3 K27 alteration. The findings reveal the existence of multiple waves of invasion and the association between somatic mutations and convergent evolution. The results provide insights for the design of future clinical trials.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Article
Ophthalmology
Sariah J. Allen, Kevin R. Mott, Homayon Ghiasi
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2014)
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)
