Article
Pharmacology & Pharmacy
Etienne E. Muller, Dumisile Maseko, Ranmini S. Kularatne
Summary: This study conducted phenotypic and genotypic surveillance on ACV resistance in HSV-2 derived from genital ulcer samples collected in a primary healthcare facility in South Africa. The results revealed no resistance-associated mutations, but identified several known natural polymorphisms and amino acid changes of unknown significance. All cultivable HSV-2 isolates showed susceptibility to ACV.
ANTIVIRAL RESEARCH
(2022)
Article
Pathology
Melissa Krystel-Whittemore, May P. Chan, Sara C. Shalin, Kenan J. Sauder, Amy Hudson, Ruth K. Foreman, Mai P. Hoang, Jeoffry B. Brennick, Shaofeng Yan, Rosalynn M. Nazarian
Summary: This study presents the first known report of herpes virus infecting deep stromal cells of the dermis, highlighting the importance of considering cutaneous stromal herpes in patients with atypical clinical lesions, especially when immunocompromised. Establishing the correct diagnosis is crucial for initiating therapy.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Dermatology
Deepthi Konda, Laxmisha Chandrashekar, Rahul Dhodapkar, Rajesh Nachiappa Ganesh, Devinder Mohan Thappa
Summary: This study characterized the clinical markers of herpes simplex virus (HSV) infection among patients with pemphigus vulgaris. It was found that male sex, presence of fissures, hemorrhagic crusts, erosions with angulated margins, linear erosions, and raised erythrocyte sedimentation rate were significantly associated with HSV infection. Hemorrhagic crusts and linear erosions were identified as independent predictors of HSV infection.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2023)
Article
Immunology
Malgorzata Krzyzowska, Anders Jarneborn, Karolina Thorn, Kristina Eriksson, Tao Jin
Summary: This study provides evidence that treatment with tofacitinib may increase the risk of disease aggravation and severe encephalitis in primary herpes simplex infection by impairing antiviral response induced by monocytes and microglia.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Cooper K. Hayes, Christopher K. Villota, Fiona B. McEnany, Stacey Ceron, Sita Awasthi, Moriah L. Szpara, Harvey M. Friedman, David A. Leib, Richard Longnecker, Matthew D. Weitzman, Lisa N. Akhtar
Summary: Clinical HSV-2 isolates collected from neonates with encephalitis are more neurovirulent in human neuronal cell culture and murine models as compared to isolates from neonates with skin-limited disease, suggesting that viral factors contribute to neurologic outcome following human neonatal infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Virology
Bangxing Hong, Upasana Sahu, Matthew P. Mullarkey, Balveen Kaur
Summary: Oncolytic herpes simplex virus (oHSV) shows promise as a treatment for solid tumors, but there are still barriers that limit its efficacy. This review focuses on the essential viral genes that allow for virus replication and spread, with the aim of enhancing oHSV infection and replication among tumor cells.
Article
Microbiology
Kevin Danastas, Gerry Guo, Jessica Merjane, Nathan Hong, Ava Larsen, Monica Miranda-Saksena, Anthony L. Cunningham
Summary: This study explores the effects of interferons (IFNs) on herpes simplex virus-1 (HSV-1) and reveals that IFNs have the potential to block virus release from nerve endings, thereby preventing transmission into the skin. The study also highlights the potential wider antiviral effects of IFN-γ in neurons, suggesting its role in HSV-1 reactivation. These findings identify new targets for the development of immunotherapies to impede HSV-1 spread from nerves to the skin.
Article
Andrology
Majid Komijani, Hamid Reza Momeni, Nima Shaykh-Baygloo, Ali Asghar Ghafarizadeh, Parisa Maleki, Mohammad Reza Tahsili
Summary: The rs187084 SNP of TLR9 plays a crucial role in Herpes simplex virus I&II infection and male infertility, potentially serving as a risk factor for HSV infection incidence.
Article
Medicine, Research & Experimental
Catielen Paula Pavi, Isabella Dai Pra, Rafael Dorighello Cadamuro, Isamu Kanzaki, Jhuly Wellen Ferreira Lacerda, Louis Pergaud Sandjo, Roberto Messias Bezerra, Jorge Federico Orellana Segovia, Gislaine Fongaro, Izabella Thais Silva
Summary: This study evaluated the activity of seventeen extracts derived from eight Amazonian medicinal plants against herpes simplex virus and chikungunya virus. Four extracts exhibited significant activity against both viruses, including the drug-resistant strains. Virucidal tests showed a higher level of virucidal activity compared to antiviral activity.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Virology
Mashidur Rana, Mizumi Setia, Pratima K. Suvas, Anish Chakraborty, Susmit Suvas
Summary: Reactive oxygen species (ROS) play a crucial role in herpes stromal keratitis (HSK), with neutrophils being the primary cells responsible for ROS generation. NOX2 is involved in ROS production in neutrophils. However, manipulating ROS levels in neutrophils may help alleviate the severity of HSK, and NOX2 is not the main contributor to ROS generation in neutrophils.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Mette Ratzer Freytag, Sofie Eg Jorgensen, Michelle Molgaard Thomsen, Ali Al-Mousawi, Alon Schneider Hait, David Olagnier, Jakob T. Bay, Marie Helleberg, Trine H. Mogensen
Summary: This study describes a 19-year-old woman with systemic HSV-1 infection and HLH, as well as a fatal course of neonatal herpesvirus infection postpartum. Investigation showed impaired antiviral responses in the mother's cells, potentially caused by variants in CASP8 or other noncoding regions of the genome.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Bishajit Sarkar, Md. Asad Ullah, Yusha Araf, Sowmen Das, Md. Hasanur Rahman, Abu Tayab Moin
Summary: HSV is a highly infectious virus with two types, HSV-1 and HSV-2, infecting millions worldwide. This study designed three polyvalent subunit vaccines targeting multiple strains of HSV, which showed stability and potential effectiveness through molecular docking and simulation studies. Validation of these vaccines in wet lab-based studies is still needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Chemistry, Multidisciplinary
Pradeep Chopra, Tejabhiram Yadavalli, Francesco Palmieri, Seino A. K. Jongkees, Luca Unione, Deepak Shukla, Geert-Jan Boons
Summary: Inhibition of human heparanase (Hpse) has been found to be a promising therapeutic strategy for preventing the spread of herpes simplex virus (HSV-1). The synthesis of hexa- and octasaccharide inhibitors of Hpse showed potent enzyme inhibition and the presence of 2-O-sulfation on iduronic acid was tolerated. These inhibitors not only blocked viral-induced shedding of cell-surface heparan sulfate (HS) but also inhibited cell migration and proliferation in corneal epithelial cells infected with HSV-1.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Pharmacology & Pharmacy
Yiwei Huang, Yuyun Song, Jichen Li, Changning Lv, Zhe-Sheng Chen, Zhijun Liu
Summary: Human herpes simplex viruses use cell surface receptors such as heparan sulfate, herpesvirus entry mediator, and nectin-1/-2, 3-O-sulfated heparan sulfate to enter host cells. No vaccine or curative treatment is currently available for HSV infections.
DRUG DISCOVERY TODAY
(2022)
Review
Microbiology
Rohini Krishnan, Patrick M. Stuart
Summary: Herpes simplex virus is a common viral infection that requires the development of new methods for prevention and management. While some vaccines have been developed in the past, there are currently no approved vaccines available. Various promising vaccine candidates are currently being studied and developed.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Ophthalmology
Annemijn P. A. Wierenga, Niels J. Brouwer, Maria Chiara Gelmi, Robert M. Verdijk, Marc-Henri Stern, Zeynep Bas, Kabir Malkani, Sjoerd G. van Duinen, Arupa Ganguly, Wilma G. M. Kroes, Marina Marinkovic, Gregorius P. M. Luyten, Carol L. Shields, Martine J. Jager
Summary: The study found that chromosome 3 and 8q aberrations have a larger influence on survival in patients with a light iris compared to those with a brown iris. This suggests a synergistic effect of iris pigmentation and chromosome status in regulating the oncogenic behavior of uveal melanoma. Iris color should be taken into consideration when calculating a patient's risk for developing metastases.
Correction
Medicine, General & Internal
Martine J. Jager, Carol L. Shields, Colleen M. Cebulla, Mohamed H. Abdel-Rahman, Hans E. Grossniklaus, Marc-Henri Stern, Richard D. Carvajal, Rubens N. Belfort, Renbing Jia, Jerry A. Shields, Bertil E. Damato
NATURE REVIEWS DISEASE PRIMERS
(2022)
Review
Biochemistry & Molecular Biology
Maria Chiara Gelmi, Laurien E. Houtzagers, Thomas Strub, Imene Krossa, Martine J. Jager
Summary: Microphthalmia-associated transcription factor (MITF) is an important regulator of melanogenesis and melanocyte development. It plays a dual role in promoting local proliferation and inhibiting invasion and inflammation in melanocytic cells, and loss of MITF expression is associated with increased invasion and poor prognosis. MITF also switches between high and low states in different phases of tumour development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Alma Martin, Jun Cai, Anna-Lena Schaedel, Mariena van der Plas, Martin Malmsten, Thomas Rades, Andrea Heinz
Summary: This study aims to develop fibers with enhanced mechanical strength and water stability, as well as sustained release of tetracycline hydrochloride. By using zein as core material and polycaprolactone as shell material, the researchers achieved improved cell attachment and wound closure, while maintaining continuous drug release.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Biochemical Research Methods
Michele Stofella, Simon P. Skinner, Frank Sobott, Jeanine Houwing-Duistermaat, Emanuele Paci
Summary: This study investigates the structure and dynamic structural variation in proteins through the experimental measurement of the spontaneous exchange of amide protons with deuterium in the solvent. Two experimental techniques, NMR and MS, are used to probe the exchange. NMR provides residue-specific information, while MS provides information specific to proteolytic digestion products. Different computational approaches have been proposed to extract residue-specific information from peptide-level MS data.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Statistics & Probability
Haiyan Liu, Jeanine Houwing-Duistermaat
Summary: In many studies on disease progression, the restricted detection limits of biomarkers lead to informative missing values. Current approaches either ignore this problem or use a global approach for estimating the mean function, which is time-consuming and may not be realistic. In this study, we propose novel estimators for the mean function in the presence of detection limits for both unbalanced sparse and dense data. We derive the asymptotic properties of the estimators and compare them with existing methods through simulations and a real data application. Our methods appear to perform well, particularly for dense data where they are computationally faster than existing methods.
Article
Biochemistry & Molecular Biology
Yongyun Li, Jie Yang, Qianqian Zhang, Shiqiong Xu, Wei Sun, Shengfang Ge, Xiaowei Xu, Martine J. Jager, Renbing Jia, Jianming Zhang, Xianqun Fan
Summary: This study reports the discovery of a new inhibitor, elesclomol, for uveal melanoma by transporting copper ions into mitochondria and generating reactive oxygen species in GNAQ/11-mutant cells, which inhibits cell migration and tumor growth.
Article
Chemistry, Multidisciplinary
Alma Martin, Jenny Natalie Nyman, Rikke Reinholdt, Jun Cai, Anna-Lena Schaedel, Mariena J. A. van der Plas, Martin Malmsten, Thomas Rades, Andrea Heinz
Summary: Nanofiber-reinforced hydrogels have attracted attention in biomedical engineering due to their mechanical strength, water absorption properties, and potential for promoting cell migration. This study demonstrates a one-step solution for producing nanofiber-reinforced hydrogels using zein and gelatin proteins. The resulting nanofiber-hydrogel composite showed significant improvements in swelling and physical integrity compared to hydrogels without nanofibers. Additionally, the incorporation of antimicrobial agents into the fibers proved effective against Staphylococcus aureus and Escherichia coli.
Review
Oncology
Richard D. Carvajal, Joseph J. Sacco, Martine J. Jager, David J. Eschelman, Roger Olofsson Bagge, J. William Harbour, Nicholas D. Chieng, Sapna P. Patel, Anthony M. Joshua, Sophie Piperno-Neumann
Summary: Uveal melanoma is a rare form of melanoma that occurs in the uveal tract of the eye, and it has distinct characteristics from cutaneous melanoma. Current treatments for primary uveal melanoma, such as radiotherapy and enucleation, can achieve local control in most patients, but distant metastases, especially in the liver, still occur in a significant number of cases. Until January 2022, no systemic therapy had been approved for metastatic uveal melanoma, leading to poor prognosis for these patients. However, recent studies have identified important genomic and immunological features of uveal melanoma, leading to the development of novel treatments, including tebentafusp, which has received regulatory approval.
NATURE REVIEWS CLINICAL ONCOLOGY
(2023)
Editorial Material
Pharmacology & Pharmacy
Artur Schmidtchen, Haris Mirza, Mariena J. A. van der Plas, Aftab Nadeem, Manoj Puthia
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Adriana Agnese Amaro, Rosaria Gangemi, Laura Emionite, Patrizio Castagnola, Gilberto Filaci, Martine. J. J. Jager, Enrica Teresa Tanda, Francesco Spagnolo, Matteo Mascherini, Ulrich Pfeffer, Michela Croce
Summary: It has been found that driver mutations GNAQ and GNA11 activate MAP kinase and YAP/TAZ pathways, and MEK inhibitors do not effectively block UM progression. Combined treatment of trametinib and different drugs targeting YAP/TAZ can overcome resistance. The combined treatment of trametinib and cerivastatin can inhibit the growth of BAP1 mutated and chromosome 3 monosomic uveal melanoma cell lines in vitro and in vivo.
Review
Pharmacology & Pharmacy
Ruben V. Huis V. In't Veld, Jeroen Heuts, Sen Ma, Luis J. J. Cruz, Ferry A. A. Ossendorp, Martine J. J. Jager
Summary: Photodynamic therapy (PDT) is an established, minimally invasive treatment for specific types of cancer. It generates reactive oxygen species (ROS) that induce cell death and disrupt the tumor area. PDT can also damage the tumor vasculature and release or expose damage-associated molecular patterns (DAMPs) to initiate an antitumor immune response. However, there are challenges that limit its widespread clinical application. A literature study has identified these challenges and discussed opportunities for improvement.
Article
Biochemistry & Molecular Biology
Maria Chiara Gelmi, Robert M. Verdijk, Laurien E. Houtzagers, Pieter A. van der Velden, Wilma G. M. Kroes, Gregorius P. M. Luyten, T. H. Khanh Vu, Martine J. Jager
Summary: MITF is an important regulator of melanogenesis and melanocyte development. MITF loss in uveal melanoma is associated with a shift in epithelial-to-mesenchymal transition factors and increased inflammation. Low MITF expression is also related to genetic changes such as monosomy 3/BAP1 loss and 8q gain/amplification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Jie Yin, Gangyin Zhao, Helen Kalirai, Sarah E. Coupland, Aart G. Jochemsen, Gabriel Forn-Cuni, Annemijn P. A. Wierenga, Martine J. Jager, B. Ewa Snaar-Jagalska, Arwin Groenewoud
Summary: Given the rarity of uveal melanoma and the lack of effective treatments, researchers have developed a method to collect and preserve limited tissue samples from primary tumors and metastases for advanced research and drug screening.
Article
Oncology
Thais M. L. Tong, Esther Bastiaannet, Frank M. Speetjens, Christian U. Blank, Gregorius P. M. Luyten, Martine J. Jager, Marina Marinkovic, T. H. Khanh Vu, Coen R. N. Rasch, Carien L. Creutzberg, Jan-Willem M. Beenakker, Henk H. Hartgrink, Jacobus J. J. Bosch, Emine Kilic, Nicole C. Naus, Serdar Yavuzyigitoglu, Caroline M. van Rij, Mark C. Burgmans, Ellen H. W. Kapiteijn
Summary: This study on patients with primary uveal melanoma found a shift towards the diagnosis of smaller tumors and an increase in eye-preserving treatments over time. Overall survival and cancer-specific survival were moderately improved in the second time period, but the time period was not associated with survival in multivariate analyses.
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)
