Article
Cell Biology
Romy Winkler, Marianne Quaas, Stefan Glasmacher, Uwe Wolfrum, Torsten Thalheim, Joerg Galle, Knut Krohn, Thomas M. Magin, Gabriela Aust
Summary: The expression and function of GPR115 in skin, specifically in epidermal differentiation, has been largely unknown. This study reveals that GPR115 is present in a small subset of keratinocytes in the stratified epidermis, and its expression is delayed in psoriatic skin. Deletion of GPR115 leads to reduced keratinocyte stratification, suggesting its role in epidermal differentiation. Intriguingly, endogenous GPR115 localizes along keratin filaments in a regular pattern, indicating a previously unknown function in regulating epidermal differentiation and keratin expression.
Article
Immunology
Yuan Liao, Yuwen Su, Ruifang Wu, Peng Zhang, Chun Feng
Summary: The overexpression of WT1 in psoriatic skin lesions may promote the development of psoriasis by regulating its target gene IL-1 beta. These findings provide a potential new target for psoriasis treatment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Oncology
Benjamin Z. Z. Sundqvist, Sami K. K. Kilpinen, Tom O. O. Boehling, Virve S. K. Koljonen, Harri J. J. Sihto
Summary: Merkel cell carcinoma (MCC) is a cutaneous neuroendocrine malignancy with a poor prognosis and an unknown cell of origin. By studying the most variably expressed genes, we identified three distinct molecular subgroups of MCC with prognostic relevance. We propose that the dichotomic expression of epidermis-related genes might reflect both an epidermal and a nonepidermal origin for MCPyV-positive MCC.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Review
Cell Biology
Hanxiao Yin, Mingzheng Hu, Dengwen Li
Summary: The epidermis is a barrier in the outermost layer of the skin that protects against pathogens. Basal keratinocytes, acting as stem cells, are crucial for epidermal development and skin damage recovery. Understanding the molecular mechanism of basal keratinocytes in epidermal development and stratification is important for preventing and treating skin lesions.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Genetics & Heredity
Dominique Talabot-Ayer, Alejandro Diaz -Barreiro, Ali Modarressi, Gaby Palmer
Summary: IL-38, encoded by the IL1F10 gene, is closely correlated with keratinocyte differentiation in human epidermis. This study characterized the regulation of IL1F10 expression and identified downstream enhancer regions that play a role in IL1F10 transcription. Overexpression of the transcription factors KLF4 and TAp63 beta promoted the expression of IL1F10 and differentiation markers in keratinocytes, indicating their involvement in regulating IL1F10 transcription. These findings provide insights into the coordinated transcription and epigenetic remodeling of IL1F10 in differentiated keratinocytes.
Article
Biochemistry & Molecular Biology
Hirofumi Teshima, Mayuko Endo, Yumea Furuyama, Hiroyuki Takama, Masashi Akiyama, Tokuji Tsuji, Hideki Tatsukawa, Kiyotaka Hitomi
Summary: The formation of human skin epidermis can be replicated in a 3D keratinocyte culture system with air-exposure being crucial for differentiation. An analysis of biological properties in submerged and air-liquid interphase culture systems revealed significant changes in hypoxia-inducible factor (HIF)-related genes. Despite high HIF protein levels, air-exposure treatment drastically reduced the transcriptional activity of HIF on its canonical response element, which was found to be essential for differentiation. Oxygen supplementation in submerged culture restored keratinocyte differentiation, highlighting the role of HIF in air-exposure-dependent differentiation in a 3D culture system.
Review
Cell Biology
Manami Toriyama, Ken J. Ishii
Summary: The skin, as the largest organ of the human body, acts as a physical and immunological barrier against pathogen infection. There is still little known about which cells in human skin have primary cilia and how they change in response to immune reactions or disease. Several studies have described mechanisms of cilia regulation by immune reactions and the physiological relevance of cilia regulating proliferation and differentiation of stroma cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Luca Pontiggia, Akshay Kumar Ahuja, Hesham Kamaleldin Yosef, Dominic Rutsche, Ernst Reichmann, Ueli Moehrlen, Thomas Biedermann
Summary: This study found that human suprabasal keratinocytes have proliferative capacity and can retro-differentiate into basal stem cells in a wound situation or when in contact with the basement membrane.
Article
Cell Biology
Alexandra Charruyer, Tracy Weisenberger, Hang Li, Ayman Khalifa, Andrew W. Schroeder, Annika Belzer, Ruby Ghadially
Summary: With age, the epidermis becomes thinner and less proliferative, with a shift towards more symmetric stem cell self-renewal divisions and a decrease in asymmetric divisions. The decrease in asymmetric stem cell divisions and increase in symmetric divisions may contribute to the hypoplasia of aging skin. Studies suggest that p53 plays a role in the reduced asymmetric stem cell divisions with age, and Notch signaling is required for p53-induced asymmetric stem cell divisions in aged keratinocytes.
Article
Dermatology
Audrey Progneaux, Celine Evrard, Valerie De Glas, Alix Fontaine, Celine Dotreppe, Evelyne De Vuyst, Arjen F. Nikkels, Vicente Garcia-Gonzalez, Laure Dumoutier, Catherine Lambert de Rouvroit, Yves Poumay
Summary: Atopic dermatitis (AD) is an inflammatory disease characterized by an alteration of epidermal barrier due to the release of IL-4 and IL-13. IL-2R gamma, a cytokine receptor subunit, is induced in keratinocytes and contributes to the alteration of the epidermal barrier and regulation of IL-13R alpha 2 expression. The expression of IL-2R gamma is observed in keratinocytes of AD lesional skin, suggesting its role in the disease.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soojung Shin, Seul-Gi Yoon, Miso Kim, Eun Jeong Cheon, Youngseo Jeon, Hyun Jung Lee, So-Hyang Chung
Summary: This study aimed to investigate the effects of mesenchymal stem cells (hMSCs) on dry eyes (DE) in a Sjogren's syndrome (SS) mouse model. The results showed that treatment with hMSCs via subconjunctival or lacrimal gland injections alleviated DE symptoms by reducing clinical markers such as tear secretion and corneal staining, goblet cell counts, and inflammatory foci infiltration. In addition, the expression of proinflammatory cytokines and autophagy markers in the lacrimal glands and cornea decreased in the hMSCs-treated mice. These findings suggest the therapeutic potentials of hMSCs in SS-related DE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Michal J. Sobkowiak, Dominic Paquin-Proulx, Lidija Bosnjak, Markus Moll, Margaret Sallberg Chen, Johan K. Sandberg
Summary: The IL-15/IL-15R-alpha complex on the surface of keratinocytes is rapidly upregulated in response to HSV-1 infection, but gradually declines beyond 24 hours due to viral interference. The shutdown of the IL-15 axis by HSV-1 represents a novel immune evasion mechanism that can be inhibited by the host iNKT cell response.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Cell Biology
Sergiu A. A. Lucaciu, Rhett Figliuzzi, Ruth Neumann, Samina Nazarali, Luigi Del Sordo, Stephanie E. E. Leighton, Alexandra Hauser, Qing Shao, Danielle Johnston, Donglin Bai, Dale W. W. Laird
Summary: Epidermal keratinocytes contain multiple connexins that regulate epidermal homeostasis. Mutations in the GJB4 gene encoding Cx30.3 are associated with a rare skin disorder called EKVP. In this study, researchers characterized three Cx30.3 mutants and found that they were non-functional and had trafficking impairments. Co-expression of wild type Cx30.3 enhanced the assembly of mutants into gap junctions, suggesting potential therapeutic value in rescuing epidermal defects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Genetics & Heredity
Hui Xu, Jun Liu, Mengyuan Niu, Shiyu Song, Lulu Wei, Gang Chen, Yibing Ding, Yong Wang, Zhonglan Su, Hongwei Wang
Summary: The study found that patients with psoriasis had elevated levels of IL-6 and sIL-6R in their plasma, with the IL-6R SNP rs4845617 significantly associated with the disease. The supplement of sIL-6R enhanced IL-6-induced Stat3 activation in keratinocytes, accelerating the pathogenesis of psoriasis in vivo.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Biochemistry & Molecular Biology
J. Sage, J. Renault, R. Domain, K. K. Bojarski, T. Chazeirat, A. Saidi, E. Leblanc, C. Nizard, S. A. Samsonov, R. Kurfurst, G. Lalmanach, F. Lecaille
Summary: This study identified neohesperidin dihydrochalcone (NHDC) as a potent inhibitor of cathepsin S (Cat S), which plays a role in the degradation of extracellular-matrix and basement membrane. NHDC inhibited the hydrolysis of key proteins in the basement membrane and reduced the expression and activity of Cat S in human keratinocytes. Furthermore, NHDC increased the expression of the anti-inflammatory cytokine IL-10 and mediated the STAT3 signaling pathway, leading to the suppression of Cat S expression.
Article
Dermatology
Matthew J. Davis, Gokul Srinivasan, Rachael Chacko, Sophie Chen, Anish Suvarna, Louis J. Vaickus, Veronica C. Torres, Sassan Hodge, Eunice Y. Chen, Sarah Preum, Kimberley S. Samkoe, Brock C. Christensen, Matthew R. Leboeuf, Joshua J. Levy
Summary: The development and application of AI algorithms are of great significance for the removal of cSCC, as they can improve operational efficiency and accuracy, especially for moderately and poorly differentiated tumors/ neoplasms. Further improvement is needed to maintain sensitivity to surrounding tissue and determine anatomical positioning.
EXPERIMENTAL DERMATOLOGY
(2024)
Article
Dermatology
Lingjing Chen, Qing Yu, Feiying Guo, Xuewen Wang, Zhenying Cai, Qiang Zhou
Summary: This study investigated the role and mechanisms of NTS in stress-induced hair growth inhibition. The results demonstrated that NTS effectively counteracted hair growth inhibition caused by stress and regulated the expression of multiple genes related to hair growth at the transcriptional level.
EXPERIMENTAL DERMATOLOGY
(2024)