Article
Multidisciplinary Sciences
Timur Shkrigunov, Yulia Kisrieva, Natalia Samenkova, Olesya Larina, Victor Zgoda, Alexander Rusanov, Daniil Romashin, Natalia Luzgina, Irina Karuzina, Andrey Lisitsa, Natalia Petushkova
Summary: The study aimed to investigate the impact of SDS on human epidermal cells and reveal the proteins and pathways involved. Two different protocols were used for protein extraction, and the results showed that Protocol 1 was more effective in identifying differentially expressed proteins related to cellular proliferation disease, including proteins involved in the skin cancer pathway. The upregulation of cancer-associated MCM6 in SDS-induced HaCaT keratinocytes was confirmed. The study provides valuable insights into the potential carcinogenicity of SDS.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Justyna Mazurkiewicz, Aleksandra Simiczyjew, Ewelina Dratkiewicz, Magdalena Kot, Katarzyna Pietraszek-Gremplewicz, Dominika Wilk, Marcin Zietek, Rafal Matkowski, Dorota Nowak
Summary: This study provides a more in-depth understanding of the role of keratinocytes in the melanoma microenvironment, highlighting their unique secretome and mediators of invasive abilities, which could be utilized by cancer cells to support their invasion of surrounding tissues.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Multidisciplinary Sciences
Ju Hwan Kim, Dong-Jun Kang, Jun-Sang Bae, Jai Hyuen Lee, Sangbong Jeon, Hyung-Do Choi, Nam Kim, Hyung-Gun Kim, Hak Rim Kim
Summary: Exposure to 1760 MHz RF-EMF induces ROS generation in HaCaT human keratinocytes, leading to activation of MMP and phosphorylation of FoxO3a and ERK1/2 related to skin aging.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Aron Dernovics, Gyorgy Seprenyi, Zsolt Razga, Ferhan Ayaydin, Zoltan Vereb, Klara Megyeri
Summary: The study found that PSM alpha 3 stimulates autophagy in HaCaT keratinocytes, increasing the number of autophagosomes and promoting the development of acidic vesicular organelles. The study also showed that PSM alpha 3 decreases the phosphorylation levels of Akt1/2/3 and mTOR, while increasing the phosphorylation levels of Erk1/2.
Article
Biochemistry & Molecular Biology
Wei Song, Yuan-Jing Ren, Lu-Lu Liu, Ya-Ying Zhao, Qi-Fu Li, Hai-Bo Yang
Summary: The study revealed that curcumin induces apoptosis in HaCaT cells through both caspase-dependent and caspase-independent pathways, providing potential evidence for treating proliferative skin diseases, including skin cancer.
Article
Biochemistry & Molecular Biology
Paulo Newton Tonolli, Carlos M. Vera Palomino, Helena C. Junqueira, Mauricio S. Baptista
Summary: Visible light affects human skin in both positive and negative ways, depending on dose and wavelength. However, the molecular mechanisms underlying the interaction with endogenous photosensitizers and the subsequent biological responses are poorly understood, leading to its neglect in photoprotection strategies.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ziwen Wang, Qiaoli Wang, Wenshen Zhong, Feng Liang, Yuying Guo, Yifei Wang, Zhiping Wang
Summary: This study aimed to explore the moisturizing and antioxidant effects of Artemisia argyi essence liquid (AL) and clarify the potential mechanism underlying those effects. The results showed that AL enhanced the expression of AQP3 and HAS2 by activating the EGFR-mediated STAT3 and MAPK signaling pathways. In addition, AL played an antioxidant role by inhibiting the NF-κB signaling pathway and activating the Nrf2/HO-1 signaling pathway, thereby increasing the expression of antioxidant enzymes (GPX1, SOD2) and decreasing the production of reactive oxygen species (ROS). This study revealed the potential use of AL as a moisturizing and antioxidant cosmetic ingredient.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Wonmin Ko, Nayeon Kim, Hwan Lee, Eun-Rhan Woo, Youn-Chul Kim, Hyuncheol Oh, Dong-Sung Lee
Summary: Compounds from the root bark of Cudrania tricuspidata have shown anti-inflammatory effects on HaCaT cells treated with tumor necrosis factor-alpha+interferon-gamma. Six compounds, including steppogenin, cudraflavone C, and macluraxanthone B, were found to be the most effective in reducing chemokine levels and inhibiting NF-KB p65 translocation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
In Ah Bae, Jae Won Ha, Joon Yong Choi, Yong Chool Boo
Summary: The antioxidant effect of Korean propolis on oxidative stress in human epidermal keratinocytes exposed to air particulate matter was studied. The hydrophilic fraction of propolis showed potential in inhibiting oxidative stress induced by particulate matter and reducing toxicity.
Article
Chemistry, Multidisciplinary
Fariba Saadati, Walison Augusto da Silva Brito, Steffen Emmert, Sander Bekeschus
Summary: Research suggests that nano- and micro-plastic particles (NMPPs) may pose a potential threat to human health. In this study, non-malignant HaCaT keratinocytes were exposed to different types, concentrations, and sizes of polystyrene (PS) and poly methyl methacrylate (PMMA) particles to determine their ability to induce micronuclei (MN) formation. The results showed that most non-charged polymer particles were neither toxic nor genotoxic, while aminated particles were toxic but not genotoxic.
Article
Dermatology
Jang-Hee Oh, Woojune Hur, Na Li, Seong Jin Jo
Summary: EGFRIs suppress lipogenesis and HA metabolism, which may contribute to adverse dermatological effects, including barrier function impairment in cancer patients treated with EGFRIs.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Chemistry, Medicinal
Ekaterina A. Chingizova, Ekaterina S. Menchinskaya, Artur R. Chingizov, Evgeny A. Pislyagin, Elena Girich, Anton N. Yurchenko, Irina Guzhova, Valery V. Mikhailov, Dmitry L. Aminin, Ekaterina A. Yurchenko
Summary: This study investigated the impact of cerebroside flavuside B on human epidermal keratinocytes co-cultivated with Staphylococcus aureus, revealing its antimicrobial and anti-inflammatory properties and its ability to significantly alleviate negative consequences caused by bacterial co-cultivation.
Article
Chemistry, Multidisciplinary
Arundhati Joshi, Titinun Nuntapramote, Dorothea Brueggemann
Summary: Self-assembled fibrinogen scaffolds are promising biomaterials for mimicking blood clots. In this study, the interaction between cocultures of human dermal fibroblasts (HDFs) and HaCaT keratinocytes with different fibrinogen topographies was investigated. All fibrinogen topographies supported cell growth and maintained the native morphology and phenotype of HDFs and HaCaTs. Successive cocultures of both cell types in a multilayered structure showed high metabolic activities. Fibrinogen nanofibers were particularly attractive for skin regeneration due to their biomimetic porous architecture.
Article
Pharmacology & Pharmacy
Yuyue Zuo, Lei Dai, Li Li, Yuqiong Huang, Xinxin Liu, Xin Liu, Xiaoru Duan, Su Jiang, Guo-Min Deng, Hongxiang Chen
Summary: We found that ANGPTL4 was significantly increased in IMQ-induced psoriasiform skin of mice and could promote keratinocyte proliferation and inflammatory response via ERK1/2 and STAT3 dependent signaling pathways in psoriasis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Genetics & Heredity
Khrystyna Sych, Simon P. Nold, Josef Pfeilschifter, Rajkumar Vutukuri, Jana Meisterknecht, Ilka Wittig, Stefan Frank, Itamar Goren
Summary: In injured skin, intact insulin signaling and glucose uptake are crucial for proper wound closure. mRNA and protein analysis revealed that the expression of genes involved in glycolysis, oxidative phosphorylation, pentose phosphate shunt, and glutamine anaplerosis are boosted during wound healing. Specifically, PKM2 plays a significant role in facilitating VEGF induction in keratinocytes during wound repair.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Cell Biology
Tsu-Yang Chao, Theresa Kordass, Wolfram Osen, Stefan B. Eichmueller
Summary: The miRNAs miR-134-3p and miR-224-3p were found to directly interact with the SOX9 gene and inhibit its expression in breast cancer cells. These two miRNAs were down-regulated in breast cancer tissues, suggesting their potential role as tumor suppressors in breast cancer progression.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Oncology
Jeffrey S. Weber, Dirk Schadendorf, Michele Del Vecchio, James Larkin, Victoria Atkinson, Michael Schenker, Jacopo Pigozzo, Helen Gogas, Stephane Dalle, Nicolas Meyer, Paolo A. Ascierto, Shahneen Sandhu, Thomas Eigentler, Ralf Gutzmer, Jessica C. Hassel, Caroline Robert, Matteo S. Carlino, Anna Maria Di Giacomo, Marcus O. Butler, Eva Munoz-Couselo, Michael P. Brown, Piotr Rutkowski, Andrew Haydon, Jean-Jacques Grob, Jacob Schachter, Paola Queirolo, Luis de la Cruz-Merino, Andrew Haydon, Jean-Jacques Grob, Jacob Schachter, Paola Queirolo, Luis De La Cruz-Merino, Andre van der Westhuizen, Alexander M. Menzies, Sandra Re, Tuba Bas, Veerle de Pril, Julia Braverman, Daniel J. Tenney, Hao Tang, Georgina Long
Summary: The CheckMate 915 trial compared adjuvant nivolumab plus ipilimumab with nivolumab alone in patients with resected melanoma. The study found that there was no significant difference in recurrence-free survival between the two treatment groups.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Oncology
Lucas Schneider, Christoph Wies, Eva I. Krieghoff-Henning, Tabea-Clara Bucher, Jochen S. Utikal, Dirk Schadendorf, Titus J. Brinker
Summary: In this study, the contribution of different data types in a combined classifier for predicting BRAF mutation status in melanoma was analyzed. The multimodal classifier showed improved generalisability compared to individual classifiers.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Oncology
Alexander C. J. van Akkooi, Dirk Schadendorf, Alexander M. M. Eggermont
Summary: Sentinel lymph node biopsy (SLNB) was introduced in the 1990s to identify patients who may benefit from completion lymph node dissection. SLNB staging has been found to be the best for staging melanoma. Adjuvant systemic therapy and biomarkers are emerging, which might reduce the need for SLNB staging in the future.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Dermatology
Jan-Malte Placke, Delphine Mertens, Alpaslan Tasdogan, Eleftheria Chorti, Dirk Schadendorf, Selma Ugurel, Alexander Roesch, Ingo Stoffels, Joachim Klode
Summary: This study demonstrates that multispectral optoacoustic tomography (MSOT) can differentiate between benign and malignant lymphadenopathy by measuring the levels of oxyhemoglobin and deoxyhemoglobin in lymph nodes. It offers a noninvasive approach to avoid unnecessary lymph node biopsies and surgeries.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Oncology
Antonino A. A. Pane, Theresa Kordass, Agnes Hotz-Wagenblatt, Elke Dickes, Annette Kopp-Schneider, Rainer Will, Barbara Seliger, Wolfram Osen, Stefan B. B. Eichmueller
Summary: This study identified several miRNA species that can affect the susceptibility of melanoma cells to T cell-mediated killing. These miRNAs may represent attractive candidates for novel therapy approaches against melanoma and other tumors.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Infectious Diseases
Laven Mavarani, Sarah Albayrak-Rena, Anja Potthoff, Martin Hower, Sebastian Dolff, Stefanie Sammet, Felix Maischack, Dirk Schadendorf, Boerge Schmidt, Stefan Esser
Summary: Our study evaluated the changes in weight, body-mass-index (BMI), and waist-to-hip ratio (WHR) over a 5-year follow-up in people living with HIV (PLH) compared to the general population. The results showed that PLH had greater increases in weight and BMI after 5 years, but lower WHR change compared to the general population.
Article
Oncology
Filippo de Braud, Christophe Dooms, Rebecca S. Heist, Celeste Lebbe, Martin Wermke, Anas Gazzah, Dirk Schadendorf, Piotr Rutkowski, Juergen Wolf, Paolo A. Ascierto, Ignacio Gil-Bazo, Shumei Kato, Maria Wolodarski, Meredith McKean, Eva Munoz Couselo, Martin Sebastian, Armando Santoro, Vesselina Cooke, Luca Manganelli, Kitty Wan, Anil Gaur, Jaeyeon Kim, Giordano Caponigro, Xuan-Mai Couillebault, Helen Evans, Catarina D. Campbell, Sumit Basu, Michele Moschetta, Adil Daud
Summary: This study explores a targeted therapy for treating NRAS-mutant melanoma, showing promising antitumor activity in patients with the use of LXH254 and trametinib in combination.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Oncology
Paolo Antonio Ascierto, Evan J. Lipson, Reinhard Dummer, James Larkin, Georgina V. Long, Rachel E. Sanborn, Vanna Chiarion-Sileni, Brigitte Dreno, Stephane Dalle, Dirk Schadendorf, Margaret K. Callahan, Marta Nyakas, Victoria Atkinson, Carlos Alberto Gomez-Roca, Naoya Yamazaki, Hussein A. Tawbi, Naomey Sarkis, Deepti Warad, Sonia Dolfi, Priyam Mitra, Satyendra Suryawanshi, Jean-Jacques Grob
Summary: Preliminary research shows that nivolumab and relatlimab have better efficacy in treating advanced melanoma patients who have previously received anti-PD-(L)1 treatment. The RELATIVITY-047 trial demonstrated that nivolumab and relatlimab, compared to nivolumab alone, significantly improved progression-free survival in previously untreated advanced melanoma patients.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Oncology
Dirk Schadendorf, Hussein Tawbi, Evan J. Lipson, F. Stephen Hodi, Piotr Rutkowski, Helen Gogas, Christopher D. Lao, Jean-Jacques Grob, Andriy Moshyk, Jennifer Lord-Bessen, Melissa Hamilton, Shien Guo, Ling Shi, Sarah Keidel, Georgina Long
Summary: The RELATIVITY-047 trial demonstrates that the fixed-dose combination of nivolumab plus relatlimab improves progression-free survival in patients with advanced melanoma while maintaining stable health-related quality of life. Although the combination treatment is associated with a higher incidence of severe adverse events, patients' discomfort with these events is low, supporting NIVO + RELA as a first-line treatment option.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Oncology
Philipp Jansen, Daniel Otero Baguer, Nicole Duschner, Jean Le'Clerc Arrastia, Maximilian Schmidt, Jennifer Landsberg, Joerg Wenzel, Dirk Schadendorf, Eva Hadaschik, Peter Maass, Joerg Schaller, Klaus Georg Griewank
Summary: In this study, a deep learning method was used to analyze histological tissue sections of melanoma patients, enabling accurate detection of lymph node metastasis and providing valuable reference for pathologists. The method was able to identify the size and classify the tumor, which is of great significance for prognosis assessment and treatment planning.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Oncology
Carola Berking, Elisabeth Livingstone, Dirk Debus, Carmen Loquai, Michael Weichenthal, Ulrike Leiter, Felix Kiecker, Peter Mohr, Thomas K. Eigentler, Janina Remy, Katharina Schober, Markus V. Heppt, Imke von Wasielewski, Dirk Schadendorf, Ralf Gutzmer
Summary: The outcome of advanced melanoma patients has greatly improved over the past 15 years. Targeted therapies blocking BRAF or inhibiting immune checkpoints have shown significant efficacy in improving overall survival. The COMBI-r study confirms the efficacy of dabrafenib plus trametinib in routine clinical practice and provides additional data in patients with brain metastases or previous treatments.
Article
Oncology
Esmee P. Hoefsmit, Franziska Vollmy, Elisa A. Rozeman, Irene L. M. Reijers, Judith M. Versluis, Liesbeth Hoekman, Alexander C. J. van Akkooi, Georgina Long, Dirk Schadendorf, Reinhard Dummer, Maarten Altelaar, Christian U. Blank
Summary: Taking into account the higher response rates of neoadjuvant immune checkpoint blockade (ICB) in stage III melanoma compared to stage IV disease, researchers hypothesized that systemic immune suppression may be responsible for lower response rates and disease recurrence. Through proteomic profiling, they found that the protein LRG1 is associated with worse prognosis and can serve as a biomarker for identifying high-risk patients and as a potential target for neoadjuvant ICB therapy.
CANCER RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Philipp Jansen, Jean Le 'Clerc Arrastia, Daniel Otero Baguer, Maximilian Schmidt, Jennifer Landsberg, Joerg Wenzel, Michael Emberger, Dirk Schadendorf, Eva Hadaschik, Peter Maass, Klaus Georg Griewank
Summary: This study highlights the enormous potential of artificial intelligence in pathology, showing that it can aid in the identification of rare cutaneous adnexal tumors and potentially become a standard tool in routine diagnostics.
EUROPEAN JOURNAL OF CANCER
(2024)
Article
Oncology
Paolo A. Ascierto, Reinhard Dummer, Helen J. Gogas, Ana Arance, Mario Mandala, Gabriella Liszkay, Claus Garbe, Dirk Schadendorf, Ivana Krajsova, Ralf Gutzmer, Vanna Chiarion-Sileni, Caroline Dutriaux, Jan Willem B. de Groot, Naoya Yamazaki, Carmen Loquai, Caroline Robert, Keith T. Flaherty
Summary: The purpose of this study was to evaluate the efficacy and safety of encorafenib alone or in combination with binimetinib for the treatment of advanced BRAF(V600)-mutant melanoma. The results showed that the combination of encorafenib and binimetinib improved progression-free survival and overall response rate, and had better tolerability compared to encorafenib alone.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Dermatology
Matthew J. Davis, Gokul Srinivasan, Rachael Chacko, Sophie Chen, Anish Suvarna, Louis J. Vaickus, Veronica C. Torres, Sassan Hodge, Eunice Y. Chen, Sarah Preum, Kimberley S. Samkoe, Brock C. Christensen, Matthew R. Leboeuf, Joshua J. Levy
Summary: The development and application of AI algorithms are of great significance for the removal of cSCC, as they can improve operational efficiency and accuracy, especially for moderately and poorly differentiated tumors/ neoplasms. Further improvement is needed to maintain sensitivity to surrounding tissue and determine anatomical positioning.
EXPERIMENTAL DERMATOLOGY
(2024)
Article
Dermatology
Lingjing Chen, Qing Yu, Feiying Guo, Xuewen Wang, Zhenying Cai, Qiang Zhou
Summary: This study investigated the role and mechanisms of NTS in stress-induced hair growth inhibition. The results demonstrated that NTS effectively counteracted hair growth inhibition caused by stress and regulated the expression of multiple genes related to hair growth at the transcriptional level.
EXPERIMENTAL DERMATOLOGY
(2024)
