期刊
EXPERIMENTAL CELL RESEARCH
卷 323, 期 2, 页码 263-275出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2014.03.002
关键词
ADAMTS1; Lymphangiogenesis; VEGFC; VEGFR-3; Lymphatic endothelial cell
资金
- Japan Society for the Promotion of Science [S13731]
- [23612004]
- [23390366]
- Grants-in-Aid for Scientific Research [24659590, 23612004, 23390348, 23390366] Funding Source: KAKEN
Angiogenesis and lymphangiogenesis play roles in malignant tumor progression, dissemination, and metastasis. ADAMTS1, a member of the matrix metalloproteinase family, is known to inhibit angiogenesis. Recombinant ADAMTS1 was shown to strongly inhibit angiogenesis. We investigated whether ADAMTSI inhibited lymphangiogenesis in the present study. We examined cell proliferation and cell migration in normal human dermal lymphatic microvascular endothelial cells (HMVEC-dLy) transduced with or without adenoviral human ADAMTS1 gene therapy. We then examined the VEGFC/ VEGFR3 signal transduction pathway in ADAMTS1-transduced HMVEC-dLy. Cell proliferation and tube formation in Matrigel were significantly lower with transduced ADAMTS1 than with control (non-transduced HMVEC-dLy). The phosphorylation of VEGFR3 was also attenuated by ADAMTS1 gene therapy in HMVEC-dLy. Immunoprecipitation assays revealed that ADAMTS1 formed a complex with VEGFC Our results demonstrated that ADAMTS1 inhibited lymphangiogenesis in vitro. The data highlight the new function of ADAMTS1 in the regulation of lymphangiogeneSis and the therapeutic potential of ADAMTSI in cancer therapy. (c) 2014 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据