Article
Cell Biology
Mathilde Kerherve, Sara Rosinska, Kilian Trillet, Alya Zeinaty, Magalie Feyeux, Steven Nedellec, Julie Gavard
Summary: Glioblastoma multiforme (GBM) is a rare but devastating brain tumor in adults, with current treatments being generally ineffective. This study focused on a population of self-sustaining transformed cells with stem-like properties (GSCs) within GBM, which play a crucial role in tumor initiation, growth, invasion, and recurrence. An ex vivo model was established to explore the invasive behavior of GSCs, and it was found that the glycoprotein Neuropilin-1 (NRP1) contributes to their spreading and invasion. This study proposes a reliable approach to studying invasive properties of patient glioma cells and identifies NRP1 as a mediator in this malignant process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Moses Musiime, Joan Chang, Uwe Hansen, Karl E. Kadler, Cedric Zeltz, Donald Gullberg
Summary: Increased awareness of the importance of extracellular matrix composition has led to a renewed interest in understanding how cells recognize and remodel the matrix. Fibronectin and fibrillar collagens are key proteins in the matrix, with integrins directly anchoring cells to collagen and supporting collagen fibrillogenesis.
Article
Cell Biology
Hongjiang Si, Na Zhao, Andrea Pedroza, Ana-Maria Zaske, Jeffrey M. Rosen, Chad J. Creighton, Kevin Roarty
Summary: Cell-extracellular matrix interactions are important in tumor progression. This study shows that the noncanonical Wnt receptor, Ror2, regulates matrix remodeling and invasion in breast cancer. Loss of Ror2 leads to disruption of E-cadherin, increased tumor cell invasion, and collagen realignment. The up-regulation and redistribution of integrins and fibronectin are also observed downstream of Ror2 loss.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Engineering, Mechanical
Jaemin Kim, Erik Mailand, Mahmut Selman Sakar, Nikolaos Bouklas
Summary: Processes such as embryonic development and wound healing involve complex interactions between cellular contractility, migration, extracellular matrix (ECM) mechanics, and remodeling. However, the regulatory principles underlying the coupled cell migration and contractility in dynamically morphing tissues are not well understood. In this study, a mechanosensitive transient nonlinear theory and a finite element implementation were proposed to capture the interplay between cell migration and contractility in 3D ECM. Simulation results from microtissue experimental setups demonstrated the changes in tissue shape and cell concentration for wounded and intact microtissues. This theoretical model and computational framework provide insights into experimental data and facilitate hypothesis-driven research.
EXTREME MECHANICS LETTERS
(2023)
Article
Engineering, Biomedical
Colin Logie, Tom van Schaik, Tilo Pompe, Katja Pietsch
Summary: The study found that fibronectin induces macrophage tolerance instead of M1 or M2 macrophage phenotypes during the differentiation from monocytes to macrophages. Immune suppression caused by fibronectin was overridden upon LPS challenge, allowing the macrophages to deploy an efficient immune response.
Review
Cell Biology
Kenneth M. Yamada, Andrew D. Doyle, Jiaoyang Lu
Summary: Cell-ECM interactions are essential in development, diseases, and tissue remodeling. Recent advances in research have provided detailed descriptions of ECM molecules, identified distinct modes of cell migration in different ECM environments, and offered new insights into organ formation mechanisms. The dynamic nature of these interactions and the feedback loops involved can contribute to disease progression.
TRENDS IN CELL BIOLOGY
(2022)
Article
Cell Biology
Katerina Karkali, Prabhat Tiwari, Anand Singh, Sham Tlili, Ignasi Jorba, Daniel Navajas, Jose J. Munoz, Timothy E. Saunders, Enrique Martin-Blanco
Summary: During development, organs can achieve precise shapes and sizes not only through growth but also through condensation. A study on the embryonic ventral nerve cord (VNC) in Drosophila embryos reveals that the condensation process occurs through oscillatory contractions. The mechanical properties of the VNC vary spatially and temporally, and forces along its longitudinal axis are heterogeneous. This condensation process depends on the coordinated mechanical activities of neurons and glia, and can be explained by a viscoelastic model.
DEVELOPMENTAL CELL
(2022)
Article
Biochemistry & Molecular Biology
Carina Magdaleno, Trenton House, Jogendra S. Pawar, Sophia Carvalho, Narendiran Rajasekaran, Archana Varadaraj
Summary: This study explores the impact of fibronectin (FN) matrix on epithelial functions in the context of cancer progression, showing that FN fibril formation decreases during hypoxia, leading to reduced migratory potential. Additionally, disruption of FN fibrils in normal breast cells results in acinar luminal filling, indicating a loss of apicobasal polarity.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Mellissa C. Alcantara, Kentaro Suzuki, Alvin R. Acebedo, Daiki Kajioka, Satoshi Hirohata, Tsuneyasu Kaisho, Yu Hatano, Kazuo Yamagata, Satoru Takahashi, Gen Yamada
Summary: The study demonstrates that androgen induces cell migration through the transcription factor MafB, which remodels the local extracellular matrix (ECM) and leads to increased cell contractility and focal adhesion assembly. Additionally, matrix metalloproteinase Mmp11, as a target gene of MafB under androgen signaling, plays a role in ECM remodeling.
Article
Engineering, Biomedical
Chang Liu, Ryan Y. Nguyen, Gabriela A. Pizzurro, Xingjian Zhang, Xiangyu Gong, Alejandro Rossello Martinez, Michael Mak
Summary: 3D in vitro tumor models have been explored for their ability to replicate key features of the tumor microenvironment. We introduced a method to modulate the collagen architecture by disrupting fibrillogenesis and gelation through mechanical agitation, generating collagen scaffolds with thicker and wavy structures. These modified scaffolds promoted tumor cell dissemination and induced differences in cell morphology and migratory behavior.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Physical
Polina Bikmulina, Nastasia Kosheleva, Yuri Efremov, Artem Antoshin, Zahra Heydari, Valentina Kapustina, Valery Royuk, Vasily Mikhaylov, Victor Fomin, Massoud Vosough, Peter Timashev, Yury Rochev, Anastasia Shpichka
Summary: Cell viability is an important parameter for evaluating tissue equivalents and chemicals, and analyzing 3D cell systems requires a combination of multiple methods to obtain accurate results.
Article
Multidisciplinary Sciences
Uiyoung Han, Wijin Kim, Hyeonjin Cha, Ju Hyun Park, Jinkee Hong
Summary: The method of engineering the iPSC membrane to stimulate the inner cells of EBs while preserving cellular activities is reported in this study. The nano-coating on iPSCs allows them to retain their properties such as adhesion capability, proliferation, and pluripotency during aggregation, without inducing lineage-specific differentiation but increasing E-cadherin expression to promote EB development. This provides a foundation for future production of sophisticated patient-specific multicellular aggregates through modification of living cell membranes.
Article
Cell Biology
Erik Brauer, Tobias Lange, Daniela Keller, Sophie Goerlitz, Simone Cho, Jacqueline Keye, Manfred Gossen, Ansgar Petersen, Uwe Kornak
Summary: Tissue formation and healing require cell proliferation, migration, extracellular matrix production, and tensioning. Cellular senescence has been found to modulate cell mechanics and extracellular matrix expression, affecting tissue stiffness and contraction. This study highlights the active mechanical role of cellular senescence in tissue formation and emphasizes the importance of a functional extracellular matrix network.
Article
Cell Biology
Irene Gimeno-LLuch, Maria Benito-Jardon, Gemma Guerrero-Barbera, Natalia Burday, Mercedes Costell
Summary: Skin injuries can lead to either regeneration or scarring, and the process is influenced by physical forces transmitted by integrins. Fibronectin (FN) plays a crucial role in repairing skin wounds and its synergy site is essential for cell adhesion and resistance to physical forces. In the absence of the FN synergy site, there are defects in keratinocyte migration and reduced fibrotic responses in skin.
Review
Medicine, Research & Experimental
Yangdan Liu, Chiakang Ho, Dongsheng Wen, Jiaming Sun, Lu Huang, Ya Gao, Qingfeng Li, Yifan Zhang
Summary: The self-renewal ability of stem cells is crucial for skin homeostasis, aging, and wound repair. COL17, as a component of the stem cell niche, plays a pivotal role in regulating stem cell maintenance and function.
Article
Cell Biology
Bruno da Rocha-Azevedo, Chin-Han Ho, Frederick Grinnell
MOLECULAR BIOLOGY OF THE CELL
(2015)
Review
Parasitology
Juliana Perrone de Menezes, Elvira M. Saraiva, Bruno da Rocha-Azevedo
PARASITES & VECTORS
(2016)
Editorial Material
Cell Biology
Bruno da Rocha-Azevedo, Sandra L. Schmid
Review
Oncology
Bruno da Rocha-Azevedo, Frederick Grinnell
EXPERIMENTAL CELL RESEARCH
(2013)
Article
Parasitology
Bruno da Rocha-Azevedo, Melissa Jamerson, Guy A. Cabral, Francine Marciano-Cabral
EXPERIMENTAL PARASITOLOGY
(2010)
Article
Microbiology
Leandro Lemgruber, Pietro Lupetti, Wanderley De Souza, Rossiane C. Vommaro, Bruno da Rocha-Azevedo
FEMS MICROBIOLOGY LETTERS
(2010)
Article
Microbiology
Bruno Da Rocha-Azevedo, Melissa Jamerson, Guy A. Cabral, Fernando C. Silva-Filho, Francine Marciano-Cabral
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2009)
Article
Microbiology
Debora Barreiros Petropolis, Juliany Cola Fernandes Rodrigues, Bruno da Rocha-Azevedo, Fernando Costa e Silva-Filho
Article
Microbiology
Melissa Jamerson, Bruno da Rocha-Azevedo, Guy A. Cabral, Francine Marciano-Cabral
Article
Multidisciplinary Sciences
Chandirasegaran Massilamany, Francine Marciano-Cabral, Bruno da Rocha-Azevedo, Melissa Jamerson, Arunakumar Gangaplara, David Steffen, Rana Zabad, Zsolt Illes, Raymond A. Sobel, Jay Reddy
Article
Cell Biology
Bruno da Rocha-Azevedo, Sungsoo Lee, Aparajita Dasgupta, Anthony R. Vega, Luciana R. de Oliveira, Tae Kim, Mark Kittisopikul, Zachariah A. Malik, Khuloud Jaqaman
Article
Microbiology
Bruno da Rocha-Azevedo, Fernando Costa e Silva-Filho
ARCHIVES OF MICROBIOLOGY
(2007)
Article
Parasitology
B. Rocha-Azevedo, M. Jamerson, G. A. Cabral, F. C. Silva-Filho, F. Marciano-Cabral
Article
Immunology
B da Rocha-Azevedo, GC Menezes, FCE Silva
MICROBIAL PATHOGENESIS
(2006)