期刊
EXPERIMENTAL CELL RESEARCH
卷 318, 期 2, 页码 103-113出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2011.10.010
关键词
Caveolin-1; Rab11a; Rab11-FIP2; Apical recycling; MDCK; Polarized epithelial cells
资金
- NIH [DK70856, DK48370, GM073846, CA-68485, DK-20593, DK-58404, HD-15052, DK-59637, EY-08126]
Recent studies have identified caveolin-1, a protein best known for its functions in caveolae, in apical endocytic recycling compartments in polarized epithelial cells. However, very little is known about the regulation of caveolin-1 in the endocytic recycling pathway. To address this question, in the current study we compared the relationship between compartments enriched in sub-apical caveolin-1 and Rab11a, a well-defined marker of apical recycling endosomes, using polarized MDCK cells as a model. We show that caveolin-1-containing vesicles define a compartment that partially overlaps with Rab11a, and that the distribution of subapical caveolin-1 and Rab11a shows a similar dependence on microtubule disruption. Mutants of the Rab11a effector, Rab11-FIP2 also altered the localization of caveolin-1. These findings indicate that caveolin-1 is coordinately regulated with Rab11a within the apical recycling system of polarized epithelial cells, suggesting that the two proteins are components of the same pathway. (C) 2011 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据