期刊
EXPERIMENTAL CELL RESEARCH
卷 317, 期 7, 页码 1049-1059出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2010.12.022
关键词
Brain tumor stem cell; Subventricular zone.; Adult human neural stem cells; Neurospheres; Tumorsphere; Niche
资金
- Department of Neurosurgery at Oslo University Hospital
- South-Eastern Norway Regional Health Authority
- Research Council of Norway through the Cancer Stem Cell Innovation Center (CAST-SFI)
Sphere forming assays have been useful to enrich for stem like cells in a range of tumors. The robustness of this system contrasts the difficulties in defining a stem cell population based on cell surface markers. We have undertaken a study to describe the cellular and organizational composition of tumorspheres, directly comparing these to neurospheres derived from the adult human subventricular zone (SVZ). Primary cell cultures from brain tumors were found to contain variable fractions of cells positive for tumor stem cell markers (CD133 (2-93%)/SSEA1 (3-15%)/ CXCR4 (1-72%)). All cultures produced tumors upon xenografting. Tumorspheres contained a heterogeneous population of cells, but were structurally organized with stem cell markers present at the core of spheres, with markers of more mature glial progenitors and astrocytes at more peripheral location. Ultrastructural studies showed that tumorspheres contained a higher fraction of electron dense cells in the core than the periphery (36% and 19%, respectively). Neurospheres also contained a heterogeneous cell population, but did not have an organization similar to tumorspheres. Although tumorspheres clearly display irregular and neoplastic cells, they establish an organized structure with an outward gradient of differentiation. We suggest that this organization is Central in maintaining the tumor stem cell pool. (C) 2010 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据