期刊
EXPERIMENTAL CELL RESEARCH
卷 315, 期 16, 页码 2856-2868出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2009.06.014
关键词
CRMP3; Vimentin; Nuclear translocation; Neurite outgrowth; Neuronal death and excitotoxicity
Collapsin response mediator proteins (CRMPs) are key modulators of cytoskeletons during neurite outgrowth in response to chemorepulsive guidance molecules. However, their roles in adult injured neurons are not well understood. We previously demonstrated that CRMP3 underwent calcium-dependent N-terminal protein cleavage during excitotoxicity-induced neurite retraction and neuronal death. Here, we report findings that the full-length CRMP3 inhibits tubulin polymerization and neurite outgrowth in cultured mature cerebellar granule neurons, while the N-terminal truncated CRMP3 underwent nuclear translocation and caused a significant nuclear condensation. The N-terminal truncated CRMP3 underwent nuclear translocation through nuclear pores. Nuclear protein pull-down assay and mass spectrometry analysis showed that the N-terminal truncated CRMP3 was associated with nuclear vimentin. In fact, nuclear-localized CRMP3 co-localized with vimentin during glutamate-induced excitotoxicity. However, the association between the truncated CRMP3 and vimentin was not critical for nuclear condensation and neurite outgrowth since overexpression of truncated CRMP3 in vimentin null neurons did not alleviate nuclear condensation and neurite Outgrowth inhibition. Together, these studies showed CRMP3's role in attenuating neurite Outgrowth possibility through inhibiting microtubule polymerization, and also revealed its novel association with vimentin during nuclear condensation prior to neuronal death. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved.
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