期刊
EXPERIMENTAL CELL RESEARCH
卷 314, 期 20, 页码 3628-3637出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2008.09.026
关键词
Prelamin A; LAP2 alpha; Myoblast differentiation; Caveolin 3
资金
- EURO-Laminopathies [LSHM-CY-2005-018690]
- ISS Programma Italia-USA Malattie Rare [N.526D/30]
- Fondazione Carisbo, Italy
Lamin A is a nuclear lamina constituent implicated in a number of human disorders including Emery-Dreifuss muscular dystrophy. Since increasing evidence suggests a role of the lamin A precursor in nuclear functions, we investigated the processing of prelamin A during differentiation of C2C12 mouse myoblasts. We show that both protein levels and cellular localization of prelamin A are modulated during myoblast activation. Similar changes of lamin A-binding proteins emerin and LAP2 alpha were observed. Furthermore, prelamin A was found in a complex with LAP2 alpha in differentiating myoblasts. Prelamin A accumulation in cycling myoblasts by expressing unprocessable mutants affected LAP2 alpha and PCNA amount and increased caveolin 3 mRNA and protein levels, while accumulation of prelamin A in differentiated muscle cells following treatment with a farnesyl transferase inhibitor appeared to inhibit caveolin 3 expression. Our data provide evidence for a critical role of the lamin A precursor in the early steps of muscle cell differentiation. (c) 2008 Elsevier Inc. All rights reserved.
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