期刊
EXPERIMENTAL ANIMALS
卷 60, 期 2, 页码 193-196出版社
INT PRESS EDITING CENTRE INC
DOI: 10.1538/expanim.60.193
关键词
chemiluminescence; quantitative western blots; standard curve
资金
- Ministry of Health, Labour and Welfare of Japan
For accurate protein quantification when using quantitative western blot analysis with chemiluminescence reagents, standard curves are needed because of the narrow quantifiable ranges. However, they are often difficult to obtain because authentic proteins are not always available. Here we present our original and convenient method using a sample mixture as a scale to create standard curves. This method allowed us to determine the quantifiable range of target and loading control proteins, making quantitative comparisons among independent blots more reproducible. Our results indicate that using a sample mixture to create standard curves is a practical method that guarantees the accuracy and reproducibility of quantitative western blot analysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据