期刊
EVOLUTION & DEVELOPMENT
卷 10, 期 5, 页码 537-545出版社
WILEY
DOI: 10.1111/j.1525-142X.2008.00269.x
关键词
-
资金
- Howard Hughes Medical Institute Investatorship
- HHMI Predoctoral Fellowship
- Washington University in St. Louis Department of Genetics
- NIH-NHGRI [5T32HG00045]
Animal morphology evolves through alterations in the genetic regulatory networks that control development. Regulatory connections are commonly added, subtracted, or modified via mutations in cis-regulatory elements, but several cases are also known where transcription factors have gained or lost activity-modulating peptide motifs. In order to better assess the role of novel transcription factor peptide motifs in evolution, we searched for synapomorphic motifs in the homeotic selectors of Drosophila melanogaster and related insects. Here, we describe an evolutionarily novel GROUCHO (GRO)-interaction motif in the ENGRAILED (EN) selector protein. This ehIFRPF motif is not homologous to the previously characterized engrailed homology 1 (eh1) GRO-interaction motif of EN. This second motif is an insect-specific WRPW-type motif that has been maintained by purifying selection in at least the dipteran/lepidopteran lineage. We demonstrate that this motif contributes to in vivo repression of the wingless (wg) target gene and to interaction with GRO in vitro. The acquisition and conservation of this auxiliary peptide motif shows how the number and activity of short peptide motifs can evolve in transcription factors while existing regulatory functions are maintained.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据