Article
Oncology
Korie A. Grayson, Jacob M. Hope, Wenjun Wang, Cynthia A. Reinhart-King, Michael R. King
Summary: Docetaxel and cabazitaxel alone do not significantly reduce cell viability in prostate cancer cells, indicating resistance to TRAIL and taxanes individually. However, the combination of taxanes and TRAIL synergistically enhances apoptosis, with taxanes sensitizing prostate cancer cells to TRAIL-induced apoptosis. Activation of JNK by ER stress upregulates death receptor expression and plays a role in sensitizing prostate cancer cells to TRAIL-induced apoptosis.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Cell Biology
Anna-Christina Rambow, Insa Aschenbach, Sofie Hagelund, Doaa Tawfik, Jan-Paul Gundlach, Sebastian Weisse, Nicolai Maass, Anna Trauzold
Summary: Binding of TRAIL to its death domain-containing receptors TRAIL-R1 and TRAIL-R2 can induce cell death and/or pro-inflammatory signaling. The importance of TRAIL and TRAIL-R1/R2 in tumor immune surveillance and cancer biology has meanwhile been well documented. TRAIL also binds to TRAIL-R3 and TRAIL-R4, which have regulatory functions in apoptotic and non-apoptotic signaling pathways. Knockdown of TRAIL-R4 affects the activation of apoptotic and non-apoptotic pathways in cancer cells, showing opposing effects on cell death and clonogenic survival. TRAIL-R4 also regulates the expression of anti-apoptotic proteins and affects the activity of AKT, ERK, p38 and NF-kappa B. This study provides evidence for the important role of endogenous TRAIL-R4 in cancer cells and improves the understanding of the complex TRAIL/TRAIL-R system in humans.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Food Science & Technology
L. M. Reyes, M. Landgraf, P. J. A. Sobral
Summary: The study found that films containing clove essential oil exhibited the best antioxidant activity, while red propolis ethanolic extract only showed antimicrobial activity against Gram(+) bacteria. Additionally, red propolis ethanolic extract played a crucial role in affecting the UV/visible light barrier and thermal properties of the films.
FOOD PACKAGING AND SHELF LIFE
(2021)
Article
Cell Biology
Sofie Hagelund, Anna Trauzold
Summary: TRAIL plays a crucial role in tumor immune surveillance and has the potential to kill cancer cells, but faces challenges in clinical trials. The external pH influences PDAC cells' response to TRAIL, with acidic pH adaptation showing the ability to enhance TRAIL sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Amriti R. Lulla, Yan Zhou, Marie D. Ralff, Avital Lev, David T. Dicker, Wafik S. El-Deiry
Summary: TRAIL-based therapies are a promising approach for selectively killing cancer cells, but limited serum half-life hinders their clinical development. This study identifies a novel miRNA, miR-3132, which induces TRAIL and apoptosis in cancer cells via the interferon signaling pathway. This discovery provides new insights into the potential of miR-3132 as a therapeutic target for cancer treatment.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Songhao Tian, Huiting Zhao, Hongru Guo, Wei Feng, Conglin Jiang, Yusuo Jiang
Summary: This study investigated the effect of propolis ethanolic extract (PEE) on D-galactose-induced damage in mouse C2C12 cells. The results showed that PEE increased cell viability, decreased senescence-associated beta-galactosidase-positive cells, and promoted cell differentiation. PEE protected cells from oxidative stress by activating the Nrf2/HO-1 signaling pathway, and inhibited apoptosis by suppressing p38 phosphorylation and reducing p53 expression. These findings provide a theoretical basis for the use of PEE in alleviating muscle atrophy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Sirikwan Sangboonruang, Natthawat Semakul, Sureeporn Suriyaprom, Kuntida Kitidee, Jiaranai Khantipongse, Sorasak Intorasoot, Chayada Sitthidet Tharinjaroen, Usanee Wattananandkul, Bordin Butr-Indr, Ponrut Phunpae, Khajornsak Tragoolpua
Summary: This study developed a niosome-based drug delivery platform for ethanolic extract of propolis (EEP) to target Mycobacterium tuberculosis (Mtb). The platform showed antimycobacterial activity and no toxicity on alveolar macrophages.
Article
Infectious Diseases
Nermine Nefzi, Stefania Pagliari, Luca Campone, Wided Megdiche-Ksouri, Filippo Giarratana, Nicola Cicero, Graziella Ziino, Luca Nalbone
Summary: This study evaluated the chemical composition and in vitro antimicrobial and antibiofilm activity of ethanol extract of propolis (EEP) from Tunisia against different bacterial strains. The antimicrobial activity of EEP in chilled vacuum-packed salmon tartare was also assessed. The results showed that the EEP demonstrated antimicrobial efficacy against Gram-positive bacteria, especially L. monocytogenes and S. aureus. In situ analyses revealed significant antimicrobial activity when EEP was used in combination with vinegar. The study suggests that propolis could be used as a biopreservative to improve the safety and quality of food.
Article
Chemistry, Multidisciplinary
Maria Jesus Lisbona-Gonzalez, Esther Munoz-Soto, Candela Reyes-Botella, Maria Victoria Olmedo-Gaya, Javier Diaz-Castro, Jorge Moreno-Fernandez
Summary: This study demonstrates that the use of Spanish propolis extract (EEP) as an adjuvant in periodontal therapy can have prophylactic and therapeutic potential against periodontal diseases, improving clinical parameters, reducing gingival bleeding, and decreasing bacterial counts of key pathogens.
APPLIED SCIENCES-BASEL
(2021)
Article
Oncology
Khalid Rashid, Christian Roeder, Freya Goumas, Jan-Hendrik Egberts, Holger Kalthoff
Summary: In this study, the inhibition of CD95 signaling altered the chemotherapeutic effects of gemcitabine by suppressing the pro-inflammatory responses from CD95L-positive tumor cells as well as from the TNF-a and TRAIL signaling in a bi-lateral crosstalk manner.
Article
Materials Science, Multidisciplinary
Abdallah S. Abdelsattar, Aalaa Eid, Nouran Rezk, Assmaa H. Hussein, Ayman El-Shibiny
Summary: This study proposed a novel method to biosynthesize gold nanoparticles using propolis extract, and demonstrated their high efficiency in degrading dyes through experiments.
Article
Cell Biology
Rosario Yerbes, Rocio Mora-Molina, F. Javier Fernandez-Farran, Laura Hiraldo, Abelardo Lopez-Rivas, Carmen Palacios
Summary: This study reveals a previously unknown cell death mechanism triggered in glutamine-addicted tumor cells in response to glutamine metabolism limitation. The mechanism is regulated by GCN2 activation induced by glutamine starvation and involves the activation of the extrinsic apoptotic pathway mediated by TRAIL-R2.
CELL DEATH & DISEASE
(2022)
Article
Food Science & Technology
Yakup Kara, Zehra Can, Sevgi Kolayli
Summary: The study compares the phenolic compositions and antioxidant activities of propolis extracts extracted with different ethanol concentrations and extraction conditions. The results show that the ultrasonic extraction method and ethanol concentrations between 60% and 70% have higher extraction efficiency, and the ideal solvent-propolis ratio is between 1:5 and 1:10.
FOOD ANALYTICAL METHODS
(2022)
Article
Biophysics
Virginia Vadillo-Rodriguez, Marco Alejandro Cavagnola, Ciro Perez-Giraldo, Maria Coronada Fernandez-Calderon
Summary: The interaction between propolis and bacterial cells at sub-inhibitory concentrations induces changes in the physico-chemical properties of the cell surface, including volumetric charge density, electrophoretic softness, and hydrophobicity. These changes, dose-dependent and consistent with the accumulation and penetration of propolis components, suggest a structural mechanism of action resulting in cell wall damage or perturbations.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Oncology
K. M. A. Zinnah, Sang-Youel Park
Summary: The study demonstrated the mechanism behind the synergistic anticancer effect of amitriptyline and TRAIL, showing that amitriptyline increases TRAIL-induced apoptosis by upregulating death receptors DR4 and DR5. Inhibition of autophagy by amitriptyline was also shown to enhance DR4 and DR5 expression.
Article
Rehabilitation
Bartlomiej Burzynski, Tomasz Jurys, Karolina Kwiatkowska, Katarzyna Cempa, Andrzej Paradysz
Summary: Overactive Bladder Syndrome (OAB) is a common bladder problem that can be treated with various methods including medication and physiotherapy. This case study highlights the role of physiotherapy in the conservative management of OAB.
PHYSIOTHERAPY THEORY AND PRACTICE
(2023)
Article
Clinical Neurology
Natalia Morawiec, Tomasz Techmanski, Karolina Tracz, Anna Kluska, Monika Arendarczyk, Monika Baran, Bozena Adamczyk, Zenon Czuba, Joanna Bronikowska, Monika Adamczyk-Sowa
Summary: This study aimed to assess the levels of selected interleukins and proinflammatory factors in cerebrospinal fluid (CSF) among patients diagnosed with relapsing-remitting multiple sclerosis (RRMS). The results showed that IL-5 and IL-13 levels increased with the age at diagnosis of MS, while the concentration of IL-10 was lower in patients over the age of 35. The levels of IFN-gamma, TNF-alpha, IL-5, IL-10 and IL-15 increased with the longer time from the first symptoms to diagnosis. IL-2 levels were significantly higher among patients without gadolinium-enhanced (Gd(+)) MRI lesions.
CLINICAL NEUROLOGY AND NEUROSURGERY
(2023)
Review
Urology & Nephrology
Pawel Rajwa, Fahad Quhal, Benjamin Pradere, Giorgio Gandaglia, Guillaume Ploussard, Michael S. Leapman, John L. Gore, Andrzej Paradysz, Derya Tilki, Axel S. Merseburger, Todd M. Morgan, Alberto Briganti, Ganesh S. Palapattu, Shahrokh F. Shariat
Summary: Mutations in BRCA1 and BRCA2 genes are associated with increased risk of prostate cancer, with BRCA2 mutations showing a stronger association. These mutations are also linked to more aggressive disease characteristics and higher mortality rates. Screening strategies for individuals with these mutations are still a subject of debate, and the long-term impact of prostate cancer screening in this population is unknown.
NATURE REVIEWS UROLOGY
(2023)
Article
Immunology
Dariusz Kajdaniuk, Wanda Foltyn, Elzbieta Morawiec-Szymonik, Zenon Czuba, Ewa Szymonik, Beata Kos-Kudla, Bogdan Marek
Summary: The effects of specific cytokines produced by T cell subsets are diverse and depend on various factors. The balance of immune cells is necessary for immune homeostasis. Imbalances in T cell subsets can lead to autoimmune diseases. The aim of this study was to determine the cytokines and modulating factors in patients with pernicious anemia.
IMMUNOLOGIC RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Piotr Londzin, Marcin Trawczynski, Urszula Cegiela, Zenon P. Czuba, Joanna Folwarczna
Summary: The extension of human life makes it increasingly important to prevent and treat diseases of the elderly, including Alzheimer's disease (AD) and osteoporosis. This study aimed to investigate the effects of donepezil, an acetylcholinesterase inhibitor, on the musculoskeletal system in rats with normal and reduced estrogen levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Agnieszka Kula, Miriam Dawidowicz, Sylwia Mielcarska, Pawel Kiczmer, Hanna Skiba, Malgorzata Krygier, Magdalena Chrabanska, Jerzy Piecuch, Monika Szrot, Julia Robotycka, Blazej Ochman, Bogumila Strzalkowska, Zenon Czuba, Elzbieta Swietochowska, Dariusz Waniczek
Summary: This study aims to investigate the correlation between HHLA2 levels and various parameters in colorectal cancer (CRC). The results demonstrate that HHLA2 is highly expressed in CRC tumor tissues compared to adjacent noncancerous tissues. The study provides valuable insights into the role of HHLA2 as a stimulatory and inhibitory immune checkpoint in CRC and suggests the potential therapeutic value of the HHLA2-KIR3DL3/TMIGD2 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Sylwia Mielcarska, Miriam Dawidowicz, Agnieszka Kula, Pawel Kiczmer, Hanna Skiba, Malgorzata Krygier, Magdalena Chrabanska, Jerzy Piecuch, Monika Szrot, Blazej Ochman, Julia Robotycka, Bogumila Strzalkowska, Zenon Czuba, Dariusz Waniczek, Elzbieta Swietochowska
Summary: Colorectal cancer (CRC) is a common malignant tumor with a high mortality rate. This study found that B7H3 is upregulated in CRC tumors and correlated with M2-macrophage polarization and tumor growth-promoting cytokines. Additionally, high B7H3 expression is associated with decreased survival in CRC patients. These findings provide new insights into the role of B7H3 in CRC immunity.
Article
Cell Biology
Miriam Dawidowicz, Agnieszka Kula, Sylwia Mielcarska, Pawel Kiczmer, Hanna Skiba, Malgorzata Krygier, Magdalena Chrabanska, Jerzy Piecuch, Monika Szrot, Julia Robotycka, Blazej Ochman, Bogumila Strzalkowska, Zenon Czuba, Elzbieta Swietochowska, Dariusz Waniczek
Summary: Immunotherapies based on ICIs in CRC are only applicable to microsatellite unstable tumors, which account for approximately 15% of all CRC cases. B7H4, a new immune checkpoint belonging to the B7 family, is associated with "cold" tumors and the downregulation of various immune cell populations.
Article
Pharmacology & Pharmacy
Anna Kurek-Gorecka, Pawel Ramos, Malgorzata Klosek, Elzbieta Bobela, Zenon P. Czuba, Radoslaw Balwierz, Pawel Olczyk
Summary: Propolis is a source of compounds with strong antibacterial activity. The aim of the study was to evaluate the antibacterial effect of Polish propolis against cariogenic bacteria. The results suggest that propolis may be beneficial in preventing dental plaque and caries.
Article
Clinical Neurology
Maria Nowak-Kiczmer, Natalia Niedziela, Zenon P. Czuba, Pawel Sowa, Krzysztof Wierzbicki, Michal Lubczynski, Monika Adamczyk-Sowa
Summary: This study assessed the differences in inflammatory parameters between primary progressive multiple sclerosis (PPMS) and other types of multiple sclerosis (MS). The results showed that inflammatory processes differed between different types of MS, and serum inflammatory parameters could be promising biomarkers for differentiating disease courses and assessing treatment response.
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2023)
Article
Chemistry, Multidisciplinary
Kacper Wachol, Tadeusz Morawiec, Agnieszka Szurko, Domenico Baldi, Anna Nowak-Wachol, Joanna Smieszek-Wilczewska, Anna Mertas
Summary: Successful implantation in augmented areas requires adequate bone density and quality, as well as thorough planning. This study evaluated the healing process after implant surgery (dental implants) using dynamic surgical navigation following bone augmentation. The results showed faster healing in patients treated with the flapless technique and Navident dynamic navigation, along with lower levels of pro-inflammatory cytokines.
APPLIED SCIENCES-BASEL
(2023)
Article
Medicine, General & Internal
Magdalena Wyszynska, Aleksandra Czelakowska, Przemyslaw Rosak, Jacek Kasperski, Maria Lopacinska, Amir Ghanem, Anna Mertas, Malgorzata Skucha-Nowak
Summary: This study analyzed the impact of COVID-19 on the oral bacterial flora of patients with complete dentures using a diagnostic device that measures NO levels in exhaled air. The results showed significantly higher NO values in patients who were vaccinated but had a history of COVID-19 infection compared to those who were vaccinated and had no history of infection.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Malgorzata Klosek, Agnieszka Krawczyk-Lebek, Edyta Kostrzewa-Suslow, Ewelina Szliszka, Joanna Bronikowska, Dagmara Jaworska, Grazyna Pietsz, Zenon P. Czuba
Summary: Inflammation plays a crucial role in immune defense, but chronic inflammation may lead to neurodegenerative diseases, atherosclerosis, inflammatory bowel diseases, or cancer. Flavanones found in citrus fruits possess anti-oxidative and anti-inflammatory properties. This study demonstrates that methyl derivatives of flavanone can inhibit inflammation and modulate the production of inflammatory cytokines.
Article
Medicine, General & Internal
Bozena Adamczyk, Natalia Morawiec, Gabriela Mamak, Sylwia Boczek, Dominika Brzek, Natalia Tredota, Patryk Walocha, Zenon P. Czuba, Michal Blachut, Wojciech Bartman, Monika Adamczyk-Sowa
Summary: Multiple sclerosis (MS) is a chronic autoimmune disorder affecting the central nervous system (CNS). This study aimed to determine the levels of biomarkers in the cerebrospinal fluid (CSF) of MS patients and healthy controls, and to investigate their potential role in disease diagnosis and progression prediction. The findings suggest that certain molecules may be promising biomarkers for MS, but further research is needed to understand their precise functions.
