Article
Cardiac & Cardiovascular Systems
Ryan Chaban, David K. C. Cooper, Richard N. Pierson
Summary: The recent pig heart transplant at the University of Maryland Medical Center has sparked interest in xenotransplantation using genetically engineered pigs. By modifying pig genes and using immunosuppressive therapy, baboons have successfully survived with pig heart grafts for almost 9 months. The initial success suggests that pig hearts could be an effective bridge to allotransplantation, but their utility for destination therapy remains uncertain.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2022)
Article
Multidisciplinary Sciences
Sharni Collins, Luca Maestrini, Francis K. C. Hui, Barbara Stuart, Maiken Ueland
Summary: Human remains often contain textile materials, making them a common source of evidence. The discovery of human remains in outdoor environments increases exposure to scavenging activity and decomposition. Traditional methods for estimating postmortem interval (PMI) can be challenging, but recent research has found success in using lipid analysis to detect and monitor changes over time. This study utilized generalized linear mixed models (GLMMs) to analyze 30 lipid outcomes in combination with accumulated-degree-days (ADD), and the results indicated oleic and palmitic acids as suitable biomarkers for future use in determining PMI. The study also addressed interspecies differences between humans and pigs.
Article
Immunology
Matthieu Glorion, Florentina Pascale, Maxime Huriet, Jerome Estephan, Carla Gouin, Celine Urien, Mickael Bourge, Giorgia Egidy, Christophe Richard, Valerie Gelin, Julien De Wolf, Morgan Le Guen, Antoine Magnan, Antoine Roux, Philippe Devillier, Isabelle Schwartz-Cornil, Edouard Sage
Summary: This study investigates the baseline effects of standard corticosteroid treatment on early innate allo-responses. These findings will contribute to the optimization and improvement of lung transplantation outcomes.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Wen Hou, Shuang Yang, Jiansen Lu, Yuan Shi, Jing Chen, Decheng Chen, Fei Wang, Lei Liu
Summary: In this study, a stable intestinal hypothermic machine perfusion (HMP) system was established and it was found that HMP could significantly alleviate intestinal ischemia-reperfusion injury (IRI) and improve the outcome after intestinal transplantation (IT).
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Physiology
Junyi Yu, Che Xu, Janet S. Lee, Jonathan K. Alder, Zongmei Wen, Guifang Wang, Agustin Alejandro Gil Silva, Pablo G. Sanchez, Joseph M. Pilewski, John F. McDyer, Xingan Wang
Summary: Maintaining alveolar ventilation can extend the tolerable warm ischemia time of murine lungs at 37 degrees Celsius up to 4 hours. Nonhypoxic lungs undergoing warm ischemia-reperfusion injury exhibit early transcriptional signatures of myeloid cell recruitment and extracellular matrix proteolysis before the dysfunction of blood-gas barrier and significant tissue damage occur.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Sergio Ghidini, Giovanni Loris Alborali, Silvio De Luca, Antonio Marco Maisano, Federica Guadagno, Mauro Conter, Adriana Ianieri, Emanuela Zanardi
Summary: This study analyzed data obtained from ante- and postmortem inspections of heavy pigs slaughtered in Italy, determining the prevalence of common lesions and exploring the correlation between findings. Some conditions found during antemortem inspections were predictive of lesions reported during postmortem inspections, highlighting the potential usefulness of antemortem inspection data in characterizing farms and organizing official controls.
Review
Medicine, General & Internal
Camilla Sandrini, Simona Boito, Claudio M. Lombardi, Sophie Lombardi
Summary: Micro-computed tomography (CT) is an effective non-invasive alternative for evaluating human fetal cardiac anatomy, especially in cases of early termination of pregnancy. It shows high diagnostic accuracy and allows for multiple off-time evaluations, making it a valuable tool for educational and research purposes in the fields of biology and bioengineering.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, Legal
J. Geissenberger, B. Ehrenfellner, F. C. Monticelli, Stefan Pittner, Peter Steinbacher
Summary: The estimation of postmortem interval is crucial in forensic work, and muscle protein degradation analysis has shown potential in providing key markers for time constraints. Research indicates that using dismembered body parts as proxies for muscle protein analysis is effective in investigating influencing factors and establishing standardized models.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
Han Li, Rong-Ghi R. Chou, Ya-Shiou Chang
Summary: The purpose of this study was to compare postmortem calpain changes in porcine muscle between Lanyu native and Kaohsiung crossbred black pigs. The results showed that the decreases in calpain-1 activity, desmin content, and shear force were slower in Lanyu native black pig than in Kaohsiung crossbred black pig samples. Therefore, postmortem proteolysis and tenderization were more limited in LM from Lanyu native pigs than from Kaohsiung crossbred pigs.
ANIMAL SCIENCE JOURNAL
(2023)
Article
Cardiac & Cardiovascular Systems
Sachiko Kadowaki, M. Ahsan Siraj, Weiden Chen, Jian Wang, Marlee Parker, Anita Nagy, Chun-Po Steve Fan, Kyle Runeckles, Jing Li, Junko Kobayashi, Christoph Haller, Mansoor Husain, Osami Honjo
Summary: Exenatide, a glucagon-like peptide-1 receptor agonist, shows protective effects on ischemia-reperfusion injury in heart transplantation surgery. The experiment results demonstrate that exenatide treatment can increase myocardial oxygen consumption, reduce cardiac troponin-I release, improve diastolic function of the heart, and limit endothelial injury. Therefore, exenatide plays an important role in protecting DCD hearts.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Letter
Immunology
Sophie A. Valkenburg, Samuel M. S. Cheng, Asmaa Hachim, Malik Peiris, John Nicholls
Summary: The infectivity of SARS-CoV-2 in deceased hosts and organisms is still unclear. A study on transgenic K18 hACE2 mice showed that virus infectivity in the lungs decreased by over 96% five days after host death, along with a 48.2% decrease in RNA copies.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Environmental Sciences
Alexander W. McCumber, Yeon Ji Kim, Omoanghe Samuel Isikhuemhen, Robert M. Tighe, Claudia K. Gunsch
Summary: Using a pig model, the study showed that airborne bacteria make up the largest portion of the lung microbiome. Bacteria from bronchial samples can be correctly identified by their farm of origin, while those from alveolar samples are indistinguishable.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Food Science & Technology
Arno Germond, Annie Venien, Christine Ravel, Brayan Castulovich, Jacques Rouel, Morgane Hutin, Sara Mezelli, Sandy Lefin, Pierre-Sylvain Mirade, Thierry Astruc
Summary: Fish industry operators need to process fish at different postmortem times, which impacts product quality, safety, and economic value. This study analyzed the postmortem aging process in trout over a 15-day window and identified biomarkers that accurately predict the postmortem time. By combining different methods, such as quantitative physicochemical measurements, histological analyses, and label-free imaging, insights on postmortem aging and the assessment of trout's freshness status were obtained.
Article
Cardiac & Cardiovascular Systems
Patrick M. Pilz, Jennifer E. Ward, Wei-Ting Chang, Attila Kiss, Edward Bateh, Alokkumar Jha, Sudeshna Fisch, Bruno K. Podesser, Ronglih Liao
Summary: This article outlines the strengths, limitations, and outcomes of small and large animal models of heart failure with reduced ejection fraction currently used in basic and translational research. Animal models play a critical role in understanding the molecular mechanisms of heart failure, although no model completely recapitulates the full human disease.
CIRCULATION RESEARCH
(2022)
Article
Medicine, Legal
Janine Geissenberger, S. Pittner, B. Ehrenfellner, L. Jakob, W. Stoiber, F. C. Monticelli, P. Steinbacher
Summary: Recent research has shown that analyzing the degradation of postmortem muscle proteins can help determine the time since death in forensic investigations. This study used frozen and thawed pig limbs to investigate the effects of freezing and thawing on protein degradation and concluded that freezing and thawing has no significant impact on the degradation behavior.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2023)
Review
Oncology
Michael Eamon Kelly
Summary: Pelvic exenteration is a complex surgical procedure used for the treatment of advanced pelvic cancers. The goal is to achieve clear margins by removing adjacent or involved organs and tissues. Although the surgery can be associated with high morbidity, advancements in treatment and reconstructive techniques have led to improved recovery and quality of life outcomes for patients.
Article
Medicine, General & Internal
Wim Ceelen, Louis Sandra, Leen Van de Sande, Martin Graversen, Michael Bau Mortensen, An Vermeulen, Elke Gasthuys, Dries Reynders, Sarah Cosyns, Anne Hoorens, Wouter Willaert
Summary: In this Phase I study, the tolerability, safety, pharmacokinetics, and tumour response of nanoparticle albumin bound paclitaxel (NAB-PTX) during pressurized intraperitoneal aerosolized chemotherapy (PIPAC) were investigated. The results showed a favorable pharmacokinetic profile and promising anticancer activity of PIPAC with NAB-PTX in patients with unresectable peritoneal metastases.
Article
Gastroenterology & Hepatology
Elke Van Daele, Eefje Stuer, Hanne Vanommeslaeghe, Wim Ceelen, Piet Pattyn, Eva Pape
Summary: Minimally invasive esophagectomy (MIE) can improve the quality of life for patients with esophageal cancer, although long-term functional, nutritional, and gastrointestinal issues may persist.
Article
Biology
Hooman Salavati, Pim Pullens, Wim Ceelen, Charlotte Debbaut
Summary: This study introduces a novel computational modeling approach to account for the regional heterogeneities and lymphatic drainage effects in solid tumor perfusion and drug delivery. The results suggest that tumor size, shape, and heterogeneity have a significant impact on interstitial fluid flow and drug penetration.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Review
Oncology
Wim Ceelen, Kjetil Soreide
Summary: Surgery is crucial for the treatment of solid cancers and plays an important role in multimodal cancer management. Surgeons and multidisciplinary teams make decisions based on reliable data, preferably from trials, to provide the best care for cancer patients. Enhancing research literacy among surgical candidates is a priority for the development of the ESSO core curriculum, and a set of principles and emerging concepts is presented to guide surgical oncologists in reading, understanding, planning, and contributing to future surgeon-led cancer trials.
Article
Oncology
A. Arjona-Sanchez, A. Martinez-Lopez, M. T. Moreno-Montilla, J. Mulsow, P. Lozano-Lominchar, B. Martinez-Torres, B. Rau, E. Canbay, A. Sommariva, M. Milione, M. Deraco, O. Sgarbura, A. Torgunrud, V. Kepenekian, N. J. Carr, A. Hoorens, J. B. Delhorme, R. Wernert, D. Goere, L. Martin-Roman, S. Cosyns, K. Flatmark, B. Davidson, L. Khellaf, F. Pereira-Perez, L. Rodriguez-Ortiz, A. Ibanez-Costa, A. Romero-Ruiz
Summary: This study validates the classification of pseudomyxoma peritonei (PMP), dividing it into two subcategories that can predict the survival and disease-free survival rates of patients. The inclusion of the Ki67 proliferation index in the pathology report for these patients is shown to be significant.
Article
Nanoscience & Nanotechnology
Helena Braet, Peter-Paul Fransen, Remco Marien, Giovanna Lollo, Wim Ceelen, Chris Vervaet, Lieve Balcaen, Frank Vanhaecke, Christian Vanhove, Stefan van der Vegte, Elke Gasthuys, An Vermeulen, Patricia Y. W. Dankers, Stefaan C. De Smedt, Katrien Remaut
Summary: This study aimed to improve the treatment of peritoneal metastasis from ovarian cancer by evaluating a nanoparticle-loaded, pH-switchable supramolecular polymer hydrogel as a controlled release drug delivery system. The research demonstrated that acidifying the nebulized hydrogel solution with carbon dioxide stimulated in situ gelation, prolonging the retention of the hydrogel in the peritoneal cavity. In vitro experiments showed that the drug was gradually released from the hydrogel and maintained cytotoxicity for 10 days. Furthermore, aerosolization of the hydrogel delayed tumor progression and significantly increased overall survival time in a xenograft model of peritoneal metastasis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Oncology
Daryl K. A. Chia, Jesse Demuytere, Sam Ernst, Hooman Salavati, Wim Ceelen
Summary: The treatment of peritoneal metastases involves heated chemotherapy combined with surgery. The effects of high temperatures on cancer cells and immune cells are still unclear. This review summarizes available data and highlights the need for further study to distinguish the positive and negative effects.
Article
Engineering, Biomedical
Elly De Vlieghere, Koen Van de Vijver, Eva Blondeel, Nathan Carpentier, Rouba Ghobeira, Jarne Pauwels, Sebastian Riemann, Manon Minsart, Charlotte Fieuws, Johanna Mestach, Ans Baeyens, Nathalie De Geyter, Charlotte Debbaut, Hannelore Denys, Benedicte Descamps, Kathleen Claes, Anne Vral, Jo Van Dorpe, Kris Gevaert, Bruno G. De Geest, Wim Ceelen, Sandra Van Vlierberghe, Olivier De Wever
Summary: Background: Long-term drug evaluation often relies on rodent models. This study presents a three-dimensional tumor model that allows for long-term drug evaluation, potentially reducing the need for animal models in drug discovery.
BIOMATERIALS RESEARCH
(2023)
Letter
Surgery
Kozo Kataoka, Kanae Takahashi, Jiro Takeuchi, Kazuma Ito, Naohito Beppu, Wim Ceelen, Yukihide Kanemitsu, Yoichi Ajioka, Itaru Endo, Kiyoshi Hasegawa, Keiichi Takahashi, Masataka Ikeda
BRITISH JOURNAL OF SURGERY
(2023)
Article
Surgery
Kozo Kataoka, Kanae Takahashi, Jiro Takeuchi, Kazuma Ito, Naohito Beppu, Wim Ceelen, Yukihide Kanemitsu, Yoichi Ajioka, Itaru Endo, Kiyoshi Hasegawa, Keiichi Takahashi, Masataka Ikeda
Summary: This study compared the correlation between recurrence-free survival (RFS) and overall survival (OS) in patients who underwent surgery for colorectal liver metastases. The results showed a strong correlation between RFS and OS, regardless of the adjuvant chemotherapy regimen used.
BRITISH JOURNAL OF SURGERY
(2023)
Article
Surgery
Mathieu Vandeputte, Franky Vansteenkiste, Wim Ceelen, Celine De Meyere, Mathieu D'Hondt
Summary: We aimed to define the place of minimally invasive pancreatoduodenectomy by comparing it with the traditional open approach. The study found that laparoscopic pancreatoduodenectomy provided acceptable short-term and oncological outcomes, with a higher major complication rate compared to open pancreatoduodenectomy, but without compromising surgical safety or oncological outcomes.
LANGENBECKS ARCHIVES OF SURGERY
(2023)
Article
Biotechnology & Applied Microbiology
Hooman Salavati, Pim Pullens, Charlotte Debbaut, Wim Ceelen
Summary: This study measured the hydraulic conductivity of tumor tissue and found that it is very limited in human tumor tissue. Moreover, the values of hydraulic conductivity varied significantly between different tumor types and samples.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Education, Scientific Disciplines
Lucas M. R. Dekesel, Louise G. J. Beckers Perletti, Ann-Sophie Madeleyn, Gillian O. Oghuma, Zahra M. C. Ongena, Robbe Vermoesen, Leen Haerens, Katharina D'Herde, Wouter Willaert
Summary: This study evaluated the added value of an online module created by students in near-peer teaching (NPT) sessions at Ghent University. The results showed that completion rates of the online module were moderately correlated with spotter exam results. Students who completed all pretests and posttests scored significantly better on the spotter exam. The study concludes that the online learning module can improve students' spotter test and neuroanatomy exam scores, and that NPT is a valuable teaching resource.
MEDICAL SCIENCE EDUCATOR
(2022)
Meeting Abstract
Cell & Tissue Engineering
Severien Standaert, Kaat De Clercq, Jos Olijve, Wim Ceelen, Chris Vervaet, Valerie Vanhoorne
TISSUE ENGINEERING PART A
(2022)