Article
Biochemical Research Methods
Matthieu Chourrout, Margaux Roux, Carlie Boisvert, Coralie Gislard, David Legland, Ignacio Arganda-Carreras, Cecile Olivier, Francoise Peyrin, Herve Boutin, Nicolas Rama, Thierry Baron, David Meyronet, Emmanuel Brun, Hugo Rositi, Marlene Wiart, Fabien Chauveau
Summary: This study demonstrates the use of ethanol-induced brain sample dehydration and XPCT for the detection and morphometric analysis of A beta plaques in the hippocampus. The proposed workflow identified differences in signal intensity and 3D shape parameters among different Alzheimer mouse strains.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
Matthieu Chourrout, Hugo Rositi, Elodie Ong, Violaine Hubert, Alexandre Paccalet, Louis Foucault, Awen Autret, Barbara Fayard, Cecile Olivier, Radu Bolbos, Francoise Peyrin, Claire Crola-da-Silva, David Meyronet, Olivier Raineteau, Helene Elleaume, Emmanuel Brun, Fabien Chauveau, Marlene Wiart
Summary: This study proposes a new approach for mapping myelin fibers in the whole rodent brain with microscopic resolution without strenuous staining. The approach enhances myelinated axons in rodent and human brain tissue, detects and quantifies white-matter injuries, and quantitatively characterizes white-matter microstructure. This non-destructive approach is compatible with subsequent complementary brain sample analysis by conventional histology and may become a novel gold-standard for investigating white-matter injury in the intact brain.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Clinical Neurology
Alexandre Bani-Sadr, Marie-Camille Ruitton-Allinieu, Jean-Christophe Brisset, Francois Ducray, Bastien Joubert, Geraldine Picard, Francois Cotton
Summary: This study aimed to determine whether diffusion-weighted imaging (DWI) can help distinguish early-stage autoimmune (AI) encephalitis from herpes simplex virus (HSV) encephalitis. By comparing the MRIs of patients from a multi-center cohort of AI encephalitis and a single-center of HSV encephalitis, it was found that restricted diffusion lesions in the medial temporal lobe are a characteristic of HSV encephalitis and can aid in distinguishing it from early-stage AI encephalitis.
JOURNAL OF NEURORADIOLOGY
(2023)
Article
Multidisciplinary Sciences
Chloe Dumot, Chrystelle Po, Lucille Capin, Violaine Hubert, Elodie Ong, Matthieu Chourrout, Radu Bolbos, Camille Amaz, Celine Auxenfans, Emmanuelle Canet-Soulas, Claire Rome, Fabien Chauveau, Marlene Wiart
Summary: This study aimed to evaluate an intracerebral cell-based therapy for stroke in a preclinical setting. The observational study in rats with ischemic stroke found that side bias and axial diffusivity were the most relevant endpoints for evaluating treatment efficacy. The impact of lesion subtype and intracerebral administration on these endpoints was assessed. Sample size calculation indicated that a multicenter trial would be needed for a therapeutic trial based on these endpoints.
SCIENTIFIC REPORTS
(2022)
Review
Clinical Neurology
Manoj Kumar, Ravi Prakash Reddy Nanga, Gaurav Verma, Neil Wilson, Jean Christophe Brisset, Kavindra Nath, Sanjeev Chawla
Summary: Proton magnetic resonance spectroscopy (H-1-MRS) is a non-invasive method to analyze the biochemical profile of brain tumors. Traditional H-1-MRS methods face challenges in spatial coverage and resolution. In recent years, more advanced metabolic imaging and spectroscopic sequences have greatly influenced the field of neuro-oncologic metabolomics. This review article summarizes the potential clinical applications of emerging techniques in studying brain tumor metabolism.
FRONTIERS IN NEUROLOGY
(2022)
Article
Medical Laboratory Technology
Vincent Jahandiez, Bruno Pillot, Gabriel Bidaux, Radu Bolbos, Neven Stevic, Marlene Wiart, Michel Ovize, Laurent Argaud, Martin Cour
Summary: CypD plays a key role in ischemia-reperfusion injury after cardiac arrest. Loss of CypD can attenuate brain and cardiac injury, improve neurological function, and enhance survival when combined with therapeutic hypothermia.
TRANSLATIONAL RESEARCH
(2022)
Article
Clinical Neurology
Francois-Daniel Ardellier, Seyyid Baloglu, Magdalena Sokolska, Vincent Noblet, Francois Lersy, Olivier Collange, Jean-Christophe Ferre, Adel Maamar, Beatrice Carsin-Nicol, Julie Helms, Maleka Schenck, Antoine Khalil, Augustin Gaudemer, Sophie Caillard, Julien Pottecher, Nicolas Lefebvre, Pierre-Emmanuel Zorn, Muriel Matthieu, Jean Christophe Brisset, Clotilde Boulay, Veronique Mutschler, Yves Hansmann, Paul-Michel Mertes, Francis Schneider, Samira Fafi-Kremer, Mickael Ohana, Ferhat Meziani, Federico Bolognini, Nicolas Meyer, Tarek Yousry, Mathieu Anheim, Francois Cotton, Hans Rolf Jager, SFNR COVID grp
Summary: This study aimed to assess changes in cerebral perfusion in patients with COVID-19 and neurological manifestations. The results showed that more than 80% of patients had hypoperfusion in the brain, mainly in the temporal and frontal lobes. This suggests that COVID-19 may lead to cerebral perfusion abnormalities.
JOURNAL OF NEURORADIOLOGY
(2023)
Article
Biochemical Research Methods
Marlene Wiart, Clement Tavakoli, Violaine Hubert, Ines Hristovska, Chloe Dumot, Stephane Parola, Frederic Lerouge, Fabien Chauveau, Emmanuelle Canet-Soulas, Olivier Pascual, David P. Cormode, Emmanuel Brun, Helene Elleaume
Summary: The activation of phagocytic cells is important in neurological diseases, and tracking them in the brain using MRI and CT can help understand their role and monitor therapeutic effects. Metal-based contrast agents like gadolinium, gold, and iron can be used for tracking phagocytic cells, but they may have toxicity and accumulation issues. Despite these challenges, early results in patients with neurological diseases are promising.
JOURNAL OF NEUROSCIENCE METHODS
(2023)
Article
Biotechnology & Applied Microbiology
Frederic Lerouge, Elodie Ong, Hugo Rositi, Francis Mpambani, Lise-Prune Berner, Radu Bolbos, Cecile Olivier, Francoise Peyrin, Vinu K. Apputukan, Cyrille Monnereau, Chantal Andraud, Frederic Chaput, Yves Berthezene, Bettina Braun, Mathias Jucker, Andreas K. O. Aslund, Sofie Nystrom, Per Hammarstrom, K. Peter Nilsson, Mikael Lindgren, Marlene Wiart, Fabien Chauveau, Stephane Parola
Summary: Aim: This study aimed to propose and evaluate a new multimodal imaging agent targeting amyloid-beta (A beta) plaques in Alzheimer's disease using various imaging techniques. Materials & methods: A new generation of hybrid contrast agents was designed and extensively characterized, and animal models of Alzheimer's disease were used for evaluation through MRI, two-photon microscopy, and synchrotron x-ray phase-contrast imaging. Results & conclusion: The study achieved two different grafting densities of luminescent-conjugated polythiophene while maintaining colloidal stability and fluorescent properties. In vivo brain uptake depended on the blood-brain barrier status. Nevertheless, successful A beta targeting was observed in both transgenic mice and A beta fibril-injected rats through multimodal imaging.
Proceedings Paper
Oncology
Elias Munoz, Pierre Baudot, Van-Khoa Le, Charles Voyton, Benjamin Renoust, Danny Francis, Vladimir Groza, Jean-Christophe Brisset, Ezequiel Geremia, Antoine Iannessi, Yan Liu, Benoit Huet
Summary: This study develops a predictive model based on morphological features from CT volumes to predict pathological states. The combination of 3D morphomics and radiomics achieves state-of-the-art results for predicting lung nodule malignancy.
CANCER PREVENTION THROUGH EARLY DETECTION, CAPTION 2022
(2022)
Article
Chemistry, Multidisciplinary
Aikaterini Tsirkou, Flora Kaczorowski, Mathieu Verdurand, Rana Raffoul, Jonathan Pansieri, Isabelle Quadrio, Fabien Chauveau, Rodolphe Antoine
Summary: This work demonstrates the feasibility of nanospray-charge-detection-mass-spectrometry (CDMS) for amplifying alpha-synuclein fibrils from Parkinson's disease or Dementia with Lewy bodies patients, and its synergistic combination with RT-QuIC.
CHEMICAL COMMUNICATIONS
(2022)
