Article
Chemistry, Multidisciplinary
Jongdoo Lim, Melody Guo, Sooyun Choi, Scott J. Miller, Eric V. Anslyn
Summary: Atropisomeric scaffolds are commonly found in pharmaceuticals and their handedness is crucial for their efficacy and safety. To keep up with the fast workflow of high-throughput screening for drug discovery, a rapid enantiomeric excess (ee) analysis method based on circular dichroism (CD) was developed for N-C axially chiral triazole derivatives. The CD assay showed accurate and efficient ee determination with minimal errors.
Article
Chemistry, Multidisciplinary
Yuesong Hu, Victor Pui-Yan Ma, Rong Ma, Wenchun Chen, Yuxin Duan, Roxanne Glazier, Brian G. Petrich, Renhao Li, Khalid Salaita
Summary: This study developed a DNA-based microparticle tension sensor for investigating mechanotransduction at curved interfaces with high throughput. The method successfully mapped and measured T-cell receptor forces and platelet integrin forces, demonstrating its potential in screening drugs that modulate cellular mechanics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Xiaoyu Guan, Zhongnian Li, Yueying Zhou, Wei Shao, Daoqiang Zhang
Summary: Nanopore sequencing, as a high-throughput sequencing technology for DNA, RNA, and proteins, faces the challenge of high labeling costs for the enormous generated data. This study introduces active learning to select samples that need to be labeled, reducing the labeling costs significantly. Experimental results demonstrate that active learning can greatly reduce the labeling amount while achieving the best baseline performance for nanopore data analysis.
Article
Chemistry, Analytical
Chuyan Zhang, Mengfan Wu, Shunming Hu, Shaorui Shi, Yixiang Duan, Wenchuang Hu, Yongxin Li
Summary: This study presents a facile, sensitive, and robust biomimetic array assay by integrating luminescent copper nanoclusters (CuNCs) and entropy-driven nanomachine (EDN). By adding fructose in CuNCs synthesis, the luminescent stability of CuNCs was improved. The DNA template for CuNCs synthesis was introduced into EDN to achieve signal amplification and logical analysis of multiple miRNAs. The proposed assay showed label-free property, high sensitivity, flexible design, and high-throughput analysis, providing ideas for developing new platforms in biological analysis and clinical application.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Jan Burian, Vincent K. Libis, Yozen A. Hernandez, Liliana Guerrero-Porras, Melinda A. Ternei, Sean F. Brady
Summary: The capture of metagenomic DNA in large clone libraries allows for the study of microbial diversity that cannot be accessed using culture-dependent methods. In this study, a CRISPR counter-selection interruption circuit (CCIC) using nuclease-deficient Cas9 was developed to retrieve target clones from complex libraries. Combining modern sequencing methods with CCIC cloning enables rapid access to the genetic diversity present in natural ecosystems. A CRISPRi method was used to retrieve sequences of interest from large metagenomic libraries.
NATURE BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Chan Seok Jun, Wonsuk Lee
Summary: We present a novel optofluidic laser that utilizes DNA microdroplets, an intercalating dye, and a glass capillary ring resonator. The laser signal emitted from the target DNA allows for accurate and rapid DNA sequence analysis from an optical standpoint, with the capability to distinguish between target DNA and non-target DNA with single-base mismatches.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Pooja Chaudhary, Geeta Devi Yadav, Surendra Singh
Summary: A rapid and simple protocol for determining the enantiopurity of primary and secondary amines using chiral solvating agents and spectroscopic analysis was developed. By mixing the analyte and chiral solvating agent, well-resolved resonance peaks for both enantiomers are obtained quickly. The enantiomeric excess of 1,2-diphenylethylenediamine was successfully determined using this method, and the binding constant and ΔG values for diastereomeric complexes were calculated.
Article
Environmental Sciences
Majid Khak Pour, Reza Fotouhi, Pierre Hucl, Qianwei Zhang
Summary: This research focuses on the development of a mechatronic system, hardware and software, for a mobile, field-based HTPP for autonomous crop monitoring in wheat fields. The system can measure canopy's height, temperature, and vegetation indices, and take high-quality photos of crops. The main contribution of this study is the autonomous, reliable, and fast data collection for wheat and similar crops.
Article
Biophysics
Min Liu, Danting He, Zhihong Liu, Chengguo Hu
Summary: A light-addressable and paper-based hydrogen peroxide (H2O2) photoelectrochemical (PEC) sensor for high-throughput detection of multiple salivary metabolites is reported. The sensor has a unique solar microcell array structure with different enzymes immobilized on different microcells, allowing the detection of uric acid (UA), glucose (GLU), and lactate (LA). The scalability and versatility of the sensor can be easily extended by connecting a series of sensors in parallel or varying the enzymes.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Zhenqing Li, Pengbo Zhang, Bo Yang, Jiahui Liu, Heqi Xi, Dawei Zhang, Yoshinori Yamaguchi
Summary: The researchers proposed a high throughput and low-cost DNA concentration system based on fluorescence technology, which can measure the concentration of 24 DNA samples within 100 seconds by analyzing the self-luminescence of individual solutions in DNA test kit. This system provides a simple way for DNA concentration determination and is suitable for labs in third world countries.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Harupjit Singh, Gagandeep Singh, Navneet Kaur, Narinder Singh
Summary: This study developed a portable sensor array and reader for determining food quality, effectively detecting different amines and linearly correlating colorimetric response with biogenic amine concentrations.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Yifu Lu, Zhuohan Yu, Yunhe Wang, Zhiqiang Ma, Ka-Chun Wong, Xiangtao Li
Summary: A novel Graph-based Multiple Hierarchical Consensus Clustering (GMHCC) method is developed in this study to handle clustering of various biomolecular data, showing high effectiveness. Experiments validate the method's efficiency and provide new insights into cell developmental lineages and characterization mechanisms.
Review
Geochemistry & Geophysics
Xiuliang Jin, Pablo J. Zarco-Tejada, Urs Schmidhalter, Matthew P. Reynolds, Malcolm J. Hawkesford, Rajeev K. Varshney, Tao Yang, Chengwei Nie, Zhenhai Li, Bo Ming, Yonggui Xiao, Yongdun Xie, Shaokun Li
Summary: To meet the challenges of global population growth and climate change, crop yields need to be improved sustainably. Genomics-assisted breeding is becoming popular for ensuring food security, highlighting the importance of linking phenotype and genotype relationships. While high-throughput genotyping is cost-effective, there is a need to enhance high-throughput crop phenotyping methods and data analytical capacities.
IEEE GEOSCIENCE AND REMOTE SENSING MAGAZINE
(2021)
Article
Biochemistry & Molecular Biology
Juan Canoura, Obtin Alkhamis, Yingzhu Liu, Connor Willis, Yi Xiao
Summary: This study presents a high-throughput method for quantifying the binding affinity, specificity, and cross-reactivity of aptamers using exonuclease digestion kinetics. It was used to identify new aptamers for fentanyl and its analogs and to validate the results. This approach greatly speeds up the characterization process of aptamers and streamlines sensor development.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemical Research Methods
Niklas Tenhaef, Robert Stella, Julia Frunzke, Stephan Noack
Summary: The study introduces an automated workflow for the rational construction of plasmids and their subsequent conjugative transfer into the biotechnological platform organism Cotynebacteriunt glutamicum. The whole workflow is accompanied by a custom-made software tool, and a library of transcription factor-biosensors based on the regulator Lrp was constructed and characterized.
ACS SYNTHETIC BIOLOGY
(2021)