Article
Microbiology
Corelle A. Z. Rokicki, James R. Brenner, Alexander H. Dills, Julius J. Judd, Jemila C. Kester, Julia Puffal, Ian L. Sparks, Malavika Prithviraj, Brittany R. Anderson, Joseph T. Wade, Todd A. Gray, Keith M. Derbyshire, Sarah M. Fortune, Yasu S. Morita
Summary: Mycobacteria spatially organize their plasma membrane, with enzymes involved in envelope biosynthesis associating with the intracellular membrane domain (IMD). Through proteomic analysis and visual screening, new IMD-associated proteins have been identified, enhancing understanding of the localization and functions of proteins within the IMD.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Piotr Hapeta, Patrycja Szczepanska, Tadeusz Witkowski, Jean-Marc Nicaud, Anne-Marie Crutz-Le Coq, Zbigniew Lazar
Summary: The translation explores the use of molasses as a substrate for bioprocesses, focusing on the role of hexose transporters and hexokinase in glucose preference in Yarrowia lipolytica. Overexpression of hexokinase with hexose transporters accelerated citric acid biosynthesis and enhanced pentose phosphate pathway, leading to increased production of polyols. Additionally, overexpression of fructophilic hexokinase resulted in a shorter processing time and higher polyol yield compared to the glycerol medium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Saptarshi Ghosh, Karishma Berta Cotta, Aniket A. Hande, Moneesha Fernandes, Sarika Mehra
Summary: The emergence of antibiotic-resistant strains of Mycobacterium tuberculosis and slow development of new antibiotics has hindered TB treatment. Efflux pump inhibitors and peptide nucleic acids show potential in improving drug efficacy and countering resistance.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Varsha Patil, Vikas Jain
Summary: This study found that deletion of one copy of the atpD gene in M. smegmatis leads to an energy-compromised state, resulting in a major routing shift in electron flux through less efficient respiratory chain complexes and an increased reduced state. This alteration further modulates the biosynthesis of cell wall associated lipids and triacylglycerol (TAG), with an accumulation of TAG-containing lipid bodies. Moreover, the mutant shows overexpression of TAG-degrading lipase genes, indicating a shift towards utilizing TAG as an energy source.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Zanah K. Francis, Akeisha N. Sanders, Dean C. Crick, Sebabrata Mahapatra, M. Nurul Islam, Martin S. S. Pavelka Jr
Summary: Mycobacteria have enzymes to catalyze nonclassical 3-3 linkages in cell wall peptidoglycan. Understanding these cross-links is important for developing antibiotic therapies. Our study shows that LdtC can function as the sole enzyme for 3-3 cross-link formation in M. smegmatis and may be part of a carbapenem-resistant pathway.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Chemistry, Medicinal
Sawsan H. Alelaiwi, Jason E. Heindl, Vignesh Sivaganesh, Bela Peethambaran, James R. McKee
Summary: Tuberculosis is a deadly infectious disease with the complicating factor of multi-drug resistant strains. This study identified potent inhibitors with low cytotoxicity, showing promise for future development of candidate agents for tuberculosis treatment.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Medicinal
Nicole C. Cardoso, Kelly Chibale, Vinayak Singh
Summary: The study focuses on the interplay between cell wall biosynthesis and energy metabolism in Mycobacterium tuberculosis, highlighting their importance in drug target discovery and development.
ACS INFECTIOUS DISEASES
(2022)
Review
Biochemical Research Methods
Remi Zallot, Nils Oberg, John A. Gerlt
Summary: The ongoing expansion of protein and genome sequence databases provides opportunities for identifying novel enzymes with biotechnological applications. A suite of genomic enzymology tools has been developed to generate sequence similarity networks for exploring sequence-function space and provide genome context for protein families, enabling the generation of testable hypotheses for new functions.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Song Chen, Zhendong Chen, Lixue Lu, Yunpeng Zhao, Ronghui Zhou, Qiong Xie, Yongzhi Shu, Jun Lin, Xufen Yu, Yonghui Wang
Summary: Using PROTAC technology, researchers have discovered a highly potent BTK PROTAC compound that can effectively degrade hyperactivated B-cell receptor signaling pathway in various lymphoma cancers. Through structure-activity relationship studies, they have identified a novel CRBN-recruiting compound that can concentration-dependently induce BTK degradation and suppress cell growth with improved metabolic stability. This research provides a potential therapeutic approach for BTK-associated human cancers and diseases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Microbiology
Romain Veyron-Churlet, Jean-Michel Saliou, Camille Locht
Summary: Deciphering protein-protein interactions through BioID technology in Mycobacterium smegmatis revealed the interconnectedness of HbhA with cholesterol degradation and heme/iron pathways. The study highlights the potential of in vivo techniques to study complex protein interactions in bacteria.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Ophthalmology
Xiao-Wen Hou, Ying Wang, Chaofu Ke, Chen-Wei Pan
Summary: This study systematically summarizes metabolic biomarkers and pathways in myopia, providing new clues to understand the disease mechanisms.
Article
Infectious Diseases
Arata Sakiyama, Ken-Ichi Oinuma, Yukihiro Kaneko
Summary: This study aims to reveal the mechanism underlying the INH-dependent induction of PzaA. Through transcriptomic analysis, a gene cluster containing MSMEI_1050 was identified to play a key role in INH-dependent gene regulation.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Shihao Cheng, Qinglin Wang, Xi Chen, Jiahao Chen, Bin Wang, Dongni Chen, Dong Shen, Jinying Tian, Fei Ye, Yu Lu, Haihong Huang, Yongjun Lu, Dongfeng Zhang
Summary: In this study, new biphenyls MptpB inhibitors were developed based on rational design with the structure-based strategy, showing greatly improved MptpB inhibition. The compounds exhibited potent antituberculosis activity and verified the therapeutic potential of targeting MptpB.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Jingya Wang, Yang Bai, Zihang Zeng, Jun Wang, Ping Wang, Yongai Zhao, Weili Xu, Yun Zhu, Xiuying Qi
Summary: This study found an association between life-course smoking and metabolic syndrome (MetS), particularly among individuals under the age of 70.
DIABETOLOGY & METABOLIC SYNDROME
(2022)
Article
Immunology
Norah Abukhalid, Rajoana Rojony, Lia Danelishvili, Luiz E. Bermudez
Summary: M. avium colonizes and infects the human host through ingestion or respiratory inhalation. It forms biofilms and adapts to low oxygen and limited nutrient conditions. The pathogen relies on fatty acid metabolism and biosynthesis of aromatic amino acid and cofactors during the transition between different environments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Correction
Chemistry, Multidisciplinary
Hua Huang, Michael S. Carter, Matthew W. Vetting, Yury Patskovsky, Steven C. Almo, John A. Gerlt
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2016)
Article
Biochemistry & Molecular Biology
Michael S. Carter, Xinshuai Zhang, Hua Huang, Jason T. Bouvier, Brian San Francisco, Matthew W. Vetting, Nawar Al-Obaidi, Jeffrey B. Bonanno, Agnidipta Ghosh, Remi G. Zallot, Harvey M. Andersen, Steven C. Almo, John A. Gerlt
NATURE CHEMICAL BIOLOGY
(2018)
Article
Biology
Sara Calhoun, Magdalena Korczynska, Daniel J. Wichelecki, Brian San Francisco, Suwen Zhao, Dmitry A. Rodionov, Mattew W. Vetting, Nawar F. Al-Obaidi, Henry Lin, Mattew J. O'Meara, David A. Scott, John H. Morris, Daniel Russel, Steven C. Almo, Andrei L. Osterman, John A. Gerlt, Mattew P. Jacobson, Brian K. Shoichet, Andrej Sali
Article
Microbiology
Jason T. Bouvier, Natalia V. Sernova, Salehe Ghasempur, Irina A. Rodionova, Matthew W. Vetting, Nawar F. Al-Obaidi, Steven C. Almo, John A. Gerlt, Dmitry A. Rodionov
JOURNAL OF BACTERIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Shengkai Luo, Hua Huang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Chemistry, Multidisciplinary
Tyler M. M. Stack, Katelyn N. Morrison, Thomas M. Dettmer, Brendan Wille, Chan Kim, Ryan Joyce, Madison Jermain, Yadanar Than Naing, Khadija Bhatti, Brian San Francisco, Michael S. Carter, John A. Gerlt
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Biochemistry & Molecular Biology
C. F. Zhang, Y. P. Liu, X. X. Wu, X. S. Zhang, H. Huang
BIOCHEMISTRY-MOSCOW
(2020)
Article
Biochemistry & Molecular Biology
Yuping Liu, Siting Pan, Xinshuai Zhang, Hua Huang
Summary: Pantothenic acid is converted to beta-alanine and (R)-pantoate, and then (R)-pantoate is transformed to aldopentoate in a reconstituted pathway identified through genomic enzymology strategy. The pathway genes, common in Proteobacterial genomes, are responsible for the biodegradation of pantothenic acid.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yongxin Li, Hua Huang, Xinshuai Zhang
Summary: This study discovered the catabolic pathway of sorbitol and its 1-deoxy analogue and experimentally verified the responsible gene cluster. Furthermore, the conservation of this catabolic gene cluster was observed in most members of the Firmicutes phylum.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Qiongxiang Yan, Hua Huang, Xinshuai Zhang
Summary: This study elucidates the chemical steps of the ergothioneine sulfonate catabolic pathway and demonstrates its full pathway reconstitution. Additionally, it reveals the presence of the ergothioneine sulfonate pathway in various bacterial species.
Review
Biotechnology & Applied Microbiology
Michael S. Carter, Matthew J. Tuttle, Joshua A. Mancini, Rhett Martineau, Chia-Suei Hung, Maneesh K. Gupta
Summary: The production of traditional concrete requires a significant amount of energy and contributes to a large portion of annual CO2 emissions. Biocement, a building material that utilizes biological structures to bind particles, has the potential to compete with traditional concrete as a more environmentally friendly alternative. This review synthesizes evidence from materials science, microbiology, biochemistry, and cell biology to provide a model for the process of biocement production and suggest areas for further research.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biology
Jolene M. Garber, Harald Nothaft, Ben Pluvinage, Martin Stahl, Xiaoming Bian, Sara Porfirio, Amber Enriquez, James Butcher, Hua Huang, John Glushka, Eric Line, John A. Gerlt, Parastoo Azadi, Alain Stintzi, Alisdair B. Boraston, Christine M. Szymanski
COMMUNICATIONS BIOLOGY
(2020)
Article
Medicine, General & Internal
Deanna Dong, Joshua Carlson, Joseph Ruberwa, Thomas Snihur, Nawar Al-Obaidi, Jose Bustillo
CASE REPORTS IN MEDICINE
(2019)