Article
Food Science & Technology
Yunika Mayangsari, Mayu Okudaira, Chinatsu Mano, Yuki Tanaka, Osamu Ueda, Tomohiro Sakuta, Yoshiharu Suzuki, Yoshinari Yamamoto, Takuya Suzuki
Summary: Research showed that DMF can enhance intestinal TJ barrier integrity by increasing the expression of occludin and claudin-1, while reducing the expression of claudin-2. This regulation involves the induction of occludin protein translation via mTOR and silencing Cldn2 mRNA via miR-16-5p.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Cell Biology
Hsiao-Chin Chou, Chun-Mei Cheng, Chi-Hwa Yang, Tzu-Yin Lin, Ya-Wen Liu, Tse-Hua Tan, Yi-Rong Chen
Summary: This study revealed the importance of DUSP3 as a regulator of epithelial TJ and its decrease may be implicated in the progression of epithelial cancers.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Fisheries
Chen Feng, Xiaofeng Liu, Niewen Hu, Yiyang Tang, Mengzhe Feng, Zejun Zhou
Summary: The secretory serine protease Ssp1 in Aeromonas hydrophila plays a crucial role in its pathogenicity by increasing intestinal permeability and disrupting tight junctions. The mutant strain delta Ssp1 exhibited reduced virulence, but showed no differences in biofilm formation, swimming motility, and resistance to environmental stress.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Virology
Takayuki Abe, Yuki Marutani, Lin Deng, Chieko Matsui, Masayoshi Fukasawa, Ryosuke Suzuki, Takaji Wakita, Yoshiharu Matsuura, Ikuo Shoji
Summary: Host cells have defense mechanisms to restrict viral infection, but viruses have counteracting strategies to infect. This study found that ANX5 and PKC isoforms, including PKC alpha and PKC eta, play a role in suppressing HCV infection by regulating OCLN integrity. Disruption of OCLN integrity increases HCV infection, and HCV disrupts ANX5-mediated OCLN integrity by downregulating PKC alpha and PKC eta expression to promote viral infection.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Abigail Taylor, Mark Warner, Christopher Mendoza, Calvin Memmott, Tom LeCheminant, Sara Bailey, Colter Christensen, Julie Keller, Arminda Suli, Dario Mizrachi
Summary: The tight junction (TJ) is a structure composed of multiple proteins responsible for cell-cell adhesion in polarized endothelium and epithelium. This article introduces a new experimental method to study the membrane protein claudins (CLDNs), providing a novel approach for studying other membrane proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Ji-In Yoon, Hyewon Cho, Raok Jeon, Mi-Kyung Sung
Summary: This study evaluated the efficacy of two novel HDAC8 inhibitors, SPA3052 and SPA3074, for the treatment of DSS-induced colitis. The results showed that SPA3074 administration increased occludin expression and upregulated SOCS1 protein expression, leading to the suppression of inflammation. These findings suggest that HDAC8 might be a promising target for the development of IBD treatments, and SPA3074 could be a potential candidate for IBD therapeutics.
Article
Biotechnology & Applied Microbiology
Xiaoliang Sun, Xinhong Song, Peng Guo, Dong Zhang, Shishuai Zuo, Kang Leng, Yun Liu, Haiyang Zhang
Summary: In this study, a novel chitosan-based delivery system with magnetic targeting and sustained release properties was developed for intravesical chemotherapy. The system demonstrated enhanced antitumor activities both in vitro and in vivo by affecting the tight junctions between urothelial cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Toxicology
Mainak Chakraborty, Anupam Gautam, Oishika Das, Aaheli Masid, Moumita Bhaumik
Summary: This study found that mice exposed to arsenic during prenatal life showed significant changes in gut physiology, such as microbial imbalance, decreased expression of tight junction protein occludin, and increased gut permeability. Supplementing the mice with food containing 5% sodium butyrate was able to reverse these changes. The study suggests that prenatal arsenic exposure can have long-lasting effects on the gut of mice.
TOXICOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Koki Watanabe, Daiki Asano, Hiroko Ushikubo, Akane Morita, Asami Mori, Kenji Sakamoto, Kunio Ishii, Tsutomu Nakahara
Summary: Metformin can protect against NMDA-induced retinal neurotoxicity by activating the AMPK and MEK/ERK signaling pathways in rats. This neuroprotective effect may be partially due to inhibitory effects on inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Xiaoli Pan, Junping Pei, Aoxue Wang, Wen Shuai, Lu Feng, Faqian Bu, Yumeng Zhu, Lan Zhang, Guan Wang, Liang Ouyang
Summary: This review provides an overview of the roles of the ERKs signaling pathway in cancer and the structure-activity relationships of small molecule inhibitors targeting ERKs. It offers important insights for drug design and optimization as well as potential therapeutic strategies to overcome drug resistance.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Biochemistry & Molecular Biology
Ulrika H. Julku, Maria Jantti, Reinis Svarcbahs, Timo T. Myohanen
Summary: The study showed that PREP-mediated phosphorylation, oligomerization, and internalization of DAT are not dependent on PKC or ERK. PREP inhibition had a varying impact on ERK phosphorylation depending on cellular metabolic state, but did not affect DAT phosphorylation or function. Additionally, PREP modifications did not affect PKC activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chen-Ling Gan, Yulian Zou, Dongmei Chen, Xindong Shui, Li Hu, Ruomeng Li, Tao Zhang, Junhao Wang, Yingxue Mei, Long Wang, Mi Zhang, Yuan Tian, Xi Gu, Tae Ho Lee
Summary: The overexpression of DAPK1 has been found to increase seizure severity and neuronal cell death in epilepsy patients. Experimental results have shown that targeting DAPK1 can effectively inhibit seizures and apoptosis both in vitro and in vivo. These findings provide important insights into the pathogenesis of epilepsy and suggest a novel therapeutic strategy for treating this condition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Kyeongmin Kim, Guanghai Nan, Leejeong Kim, Minjee Kwon, Kyung Hee Lee, Myeounghoon Cha, Bae Hwan Lee
Summary: This study aimed to investigate the effects of insular cortex stimulation (ICS) on neuropathic pain and determine how ICS modulates pain. The results showed that the most effective pain relief was achieved with ICS at 50 Hz-120 μA in a single trial, and its effects lasted for 4 days after repetitive ICS termination. The expression levels of phosphorylated extracellular signal-regulated kinase (pERK), astrocytes, and microglia were increased in neuropathic rats, but they were decreased after ICS, particularly in layers 2-3 of the insular cortex where pERK and neurons colocalize.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Gastroenterology & Hepatology
Kushal Saha, Ashwinkumar Subramenium Ganapathy, Alexandra Wang, Nathan Michael Morris, Eric Suchanec, Wei Ding, Gregory Yochum, Walter Koltun, Meghali Nighot, Thomas Ma, Prashant Nighot
Summary: Inflammatory bowel disease (IBD) is associated with the loss of the protein occludin, which forms the epithelial barrier. Autophagy, a cell-survival mechanism, can upregulate occludin levels and enhance the tight junction barrier, protecting against barrier loss and inflammation-induced damage.
JOURNAL OF CROHNS & COLITIS
(2023)
Article
Cell Biology
Jinguang Yang, Lili Miao, Ye Xue, Xiaoyan Wang
Summary: In this study, the therapeutic mechanism of Yiyi Fuzi Baijiang powder (YFBP) on ulcerative colitis (UC) was investigated. Network pharmacology and bioinformatics methods were used to identify the potential targets and active ingredients of YFBP, and experimental studies were conducted to validate the findings. The results revealed that YFBP exerted therapeutic effects on UC by regulating inflammatory pathways, repairing intestinal epithelial barrier, and modulating intestinal microbiota.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2023)
Article
Biochemistry & Molecular Biology
Jiao-jiao Ji, Qi Feng, Hai-feng Sun, Xue-jun Zhang, Xiao-xiao Li, Jian-kuan Li, Jian-ping Gao
Article
Energy & Fuels
Jiankuan Li, Chong Sun, Shuo Shuang, Morteza Roostaei, Vahidoddin Fattahpour, Mandi Mahmoudi, Hongbo Zeng, Jing-Li Luo
JOURNAL OF PETROLEUM SCIENCE AND ENGINEERING
(2019)
Article
Integrative & Complementary Medicine
Jiankuan Li, Yujing Zhao, Lingya Cao, Qinghong Zheng, Jianping Gao
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2019)
Article
Materials Science, Multidisciplinary
Jiankuan Li, Chong Sun, Morteza Roostaei, Mandi Mahmoudi, Vahidoddin Fattahpour, Hongbo Zeng, Jing-Li Luo
Article
Biochemistry & Molecular Biology
Jiankuan Li, Yan Wang, Xia Zhang, Lingya Cao, Jiaojiao Ji, Qinghong Zheng, Jianping Gao
JOURNAL OF CARBOHYDRATE CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Yibo Yang, Chen Huang, Wenying Guo, Hui Zhao, Zhenjie Li, Jiankuan Li, Jianyong Bao, Hengchang Wang
APPLIED SCIENCES-BASEL
(2020)
Article
Chemistry, Medicinal
Jiankuan Li, Junqing Zhang, Lingya Cao, Jiaojiao Ji, Jianping Gao
Summary: A new cycloartanyl ester, codonopilate D, was isolated from Codonopsis pilosula roots, showing potential for inhibiting LPS-induced NO production in RAW 264.7 cells.
CHEMISTRY OF NATURAL COMPOUNDS
(2021)
Article
Integrative & Complementary Medicine
Jiankuan Li, Lina Dong, Yue Liu, Jianping Gao
Summary: The study evaluated the prebiotic effects of Codonopsis pilosula polysaccharide (CPP) on human gut bacteria in vitro. Results showed that CPP significantly stimulated the growth of genus Bifidobacterium, with potential benefits to human health. Although CPP also exhibited regulatory trends on other genera, no significant differences were observed, likely due to the limited sample size.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Chemistry, Medicinal
Jiankuan Li, Yan Wang, Junqing Zhang, Yune Bai, Jianping Gao
Summary: This study reports the presence of a beta-D-fructopyranosyl-(2 -> 1)-linked fructan in nature for the first time, with a beta-D-fructofuranosyl-(2 -> 1)-linked fructan in a mixture from Codonopsis pilosula. The molecular weights of these two fructans were determined by high-performance gel permeation chromatography (HPGPC) to be 2.33 and 1.31 kDa, and their structures were analyzed using 1D and 2D NMR.
CHEMISTRY OF NATURAL COMPOUNDS
(2021)
Article
Food Science & Technology
Jiankuan Li, Yan Wang, Jiaojiao Ji, Lingya Cao, Yune Bai, Jianping Gao
Summary: The research identified a glucan named CPC from the roots of Codonopsis pilosula, with a structure of 1,4-linked alpha-D-glucose backbone and 1,6-linked beta-D-glucose branched chains. Additionally, CPC exhibited significant induction on immune-related indicators, indicating its potential benefits for regulating human immune health.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Engineering, Environmental
Jiankuan Li, Hongbo Zeng, Jing-Li Luo
Summary: This study successfully developed a smart electroless Ni-P composite coating incorporated with pH-responsive benzotriazole (BTA)-loaded nano-capsules, which demonstrated excellent anti-corrosion performance on N80 carbon steel. The coating effectively inhibited corrosion, protected the surface, and showed a high inhibition efficiency of over 80%.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Plant Sciences
Lina Dong, Xiuzhao Fan, Qian Li, Jiankuan Li, Guangxin Chen, Dong Yang, Yafeng Li, Liping Zhao, Haizhen Yin, Xiaoyan Wang, Xiaojing Liu, Zhonghua Zhao, Shumei Lin
Summary: This study investigates the potential therapeutic value and mechanism of Anemone chinensis Bunge (AB) aqueous enema for ulcerative colitis (UC). The network pharmacology analysis demonstrated that AB may affect key targets and proteins related to UC. In a mouse model, AB aqueous enema alleviated colonic damage, restored tight junction proteins, and improved the diversity of gut microbiota.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Lingya Cao, Changli Du, Xiaolu Zhai, Jiankuan Li, Jingyi Meng, Yunyun Shao, Jianping Gao
Summary: This study investigates the treatment mechanisms of Codonopsis pilosula polysaccharides (CPP) in spleen deficiency syndrome (SDS) mice using a combination of 16S rRNA gene sequencing and targeted metabolomics. The results show that CPP improves SDS by regulating gut microbiota and energy metabolism-related pathways.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhe-Yu Liu, Jiao-Jiao Ji, Feng Jiang, Xing-Rui Tian, Jian-Kuan Li, Jian-Ping Gao
Summary: In this study, a highly efficient Agrobacterium-mediated callus genetic transformation system was successfully established for Codonopsis pilosula, providing a foundation for functional analysis of genes related to bioactive components through genetic engineering technology.
PLANT BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Chenxing Yi, Jiankuan Li, Lin -Bo Liu, Subiao Liu, Jing-Li Luo
Summary: In this study, amorphous NiCoP dendrites were homogeneously in-situ constructed on nickel foam via a smart electroless plating strategy. The NiCoP@NF exhibited high activity and stability for oxygen evolution reaction (OER), along with improved charge transfer resistance, overpotential, turnover frequency, and mass activity, compared to its counterpart. The exceptional performance of NiCoP@NF can be attributed to its unique architecture and synergistic interplay of Ni and Co.
APPLIED SURFACE SCIENCE
(2023)