Article
Biology
Chong Guo, Stephanie Rudolph, Morgan E. Neuwirth, Wade G. Regehr
Summary: In the cerebellar cortex, Purkinje cells selectively inhibit unipolar brush cells expressing mGluR1, thus regulating balance and eye movement. GABA receptor-mediated inhibition on unipolar brush cells can influence the input-output transformations of the cerebellar cortex in diverse ways.
Article
Oncology
John Charles Rotondo, Chiara Mazziotta, Carmen Lanzillotti, Chiara Stefani, Giada Badiale, Giulia Campione, Fernanda Martini, Mauro Tognon
Summary: This review summarizes the function, activation, and ligands of the purinergic P2X7 receptor (P2X7R), as well as its role in inflammation and cancer. The activation of P2X7R has been found to have both pro- and anti-tumor effects. The article also describes the current therapeutic approaches and clinical trials of P2X7R modulators.
Article
Immunology
Robson Xavier Faria
Summary: The inflammatory focus and tumor microenvironment share similarities in terms of immune cells and macrophages. Under simulated hypoxic conditions, the function of P2X7 receptors is enhanced, along with increased HIF-1 alpha levels and suppressed HIF-1 alpha antagonists. Additionally, the intracellular P2X7 receptor regulator PIP2 is activated in simulated hypoxic conditions.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anita Monteverdi, Danila Di Domenico, Egidio D'Angelo, Lisa Mapelli
Summary: In this study, the frequency dependence and spatial anisotropy of cerebellar activation were investigated using a high-throughput high-density multielectrode array. The results showed that mossy fiber inputs reached the Purkinje cell layer even at low frequencies, but the efficiency of transmission increased at higher frequencies. These findings provide important insights into the computational properties and pathological alterations of the cerebellar cortex.
Article
Biochemistry & Molecular Biology
Jaideep Kesavan, Orla Watters, Laura de Diego-Garcia, Aida Menendez Mendez, Mariana Alves, Klaus Dinkel, Michael Hamacher, Jochen H. M. Prehn, David C. C. Henshall, Tobias Engel
Summary: This study showed the functional expression of P2X7Rs in human-induced pluripotent stem cell-derived astrocytes. Stimulation with P2X7R agonists induced calcium responses in these cells, supporting their use in investigating the role of P2X7R in CNS disorders.
PURINERGIC SIGNALLING
(2023)
Article
Pharmacology & Pharmacy
Jasmin Ballout, Rebecca Classen, Katrin Richter, Veronika Grau, Martin Diener
Summary: This study found that ionotropic P2X receptors are involved in the regulation of intestinal ion transport in rat colonic epithelia.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell Biology
Joy Zhou, Meike E. E. van der Heijden, Luis E. Salazar E. Leon, Tao Lin, Lauren N. N. Miterko, Dominic J. J. Kizek, Ross M. M. Perez, Matea Paveskovic, Amanda M. M. Brown, Roy V. V. Sillitoe
Summary: This study examined the effects of propranolol on tremor and cerebellar circuit activity in mice. The results showed that propranolol can reduce both physiological and pathophysiological tremor by modulating the activity of Purkinje cells and cerebellar nuclei neurons. However, it did not have any effect on ataxia in mice with cerebellar dysfunction. These findings suggest that propranolol may be a potential therapeutic option for tremor, but further research is needed to fully understand its mechanisms of action.
Article
Biology
Wenqin Luo, Guan Ning Lin, Weichen Song, Yu Zhang, Huadong Lai, Man Zhang, Juju Miao, Xiaomu Cheng, Yongjie Wang, Wang Li, Wenxiang Wei, Wei-Qiang Gao, Ru Yang, Jia Wang
Summary: This study used single-cell spatial transcriptomics analysis to reveal the similarities and differences between developing cerebellar granule cells and medulloblastoma cells. The research identified different states of developing granule cells and found that transformed granule cells in medulloblastoma closely resemble developing granule neurons but exhibit less tendency to differentiate. This provides direct evidence for the similarities and discrepancies between normal cerebellar development and tumorigenesis.
Review
Neurosciences
Meike E. van der Heijden, Roy V. Sillitoe
Summary: Cerebellar development is a complex process involving interactions between inhibitory Purkinje cells and excitatory granule cells, with Purkinje cells considered as master regulators of the process. The connectivity and function of Purkinje cells are influenced by cues provided by granule cells, which help shape the firing properties of Purkinje cells. This dynamic interplay between different cell types is essential for the establishment of mature cerebellar circuits.
Article
Medicine, Research & Experimental
Paulo Anastacio Purtado Pacheco, Juliana Vieira Faria, Ana Claudia Silva, Natalia Lidmar von Ranke, Robson Coutinho Silva, Carlos Rangel Rodrigues, David Rodrigues da Rocha, Robson Xavier Faria
Summary: This study evaluated the pharmacological effects of eleven N, S-acetal juglone derivatives as P2x7R inhibitors. Through in vitro and in vivo experiments, we identified one derivative with promising inhibitory activity and low toxicity. Our in silico studies suggest that the 1,4-naphthoquinone moiety might be a valuable molecular scaffold for the development of novel P2x7R antagonists, consistent with our previous research.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Cell Biology
Elena De Marchi, Anna Pegoraro, Elena Adinolfi
Summary: The P2X7 receptor, known for its role in immune responses, has emerged as a critical factor in promoting cancer. Studies have shown a correlation between P2X7 alterations and lymphoproliferative and myeloproliferative diseases. Recent research suggests that P2X7 and its genetic variants could be new biomarkers in hematological malignancies, and that both P2X7 antagonists and agonists could be potential therapeutic tools.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Timothy S. Balmer, Carolina Borges-Merjane, Laurence O. Trussell
Summary: The study reveals that glutamate is maintained at a high level in the synaptic cleft of cerebellar unipolar brush cells, influencing their excitatory or inhibitory responses and modulating transmission. The ambient glutamate originates from spike-independent exocytosis at the MF terminal, dependent on the activity of glutamate transporters.
Article
Cell Biology
Fabio Grassi, Benedetta De Ponte Conti
Summary: eATP, as a danger-associated molecular pattern, plays a role in immune response by stimulating P2 receptors. However, its impact in the tumor microenvironment is complex and not well-established.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Rafael Zuniga, Daniel Mancilla, Tamara Rojas, Fernando Vergara, Wendy Gonzalez, Marcelo A. Catalan, Leandro Zuniga
Summary: Two pore domain potassium channels play an important role in excitability in the nervous system. These channels produce background K+ currents and are associated with lipid rafts, but their functional activity is independent of cholesterol membrane organization.
Article
Medicine, Research & Experimental
Xiaomei Kong, William C. Bennett, Corey M. Jania, Kelly D. Chason, Zachary German, Jennifer Adouli, Samuel D. Budney, Brandon T. Oby, Catharina van Heusden, Eduardo R. Lazarowski, Ilona Jaspers, Scott H. Randell, Barry A. Hedgespeth, Glenn Cruse, Xiaoyang Hua, Stephen A. Schworer, Gregory J. Smith, Samir Np Kelada, Stephen L. Tilley
Summary: This study shows that ozone induces mast cell degranulation and bronchial hyperresponsiveness, with P2X7 receptors playing a crucial role in the process.
Article
Biochemistry & Molecular Biology
Marina Arribas-Blazquez, Luis Alcides Olivos-Ore, Maria Victoria Barahona, Mercedes Sanchez de la Muela, Virginia Solar, Esperanza Jimenez, Javier Gualix, J. Michael McIntosh, Antonio Ferrer-Montiel, Maria Teresa Miras-Portugal, Antonio R. Artalejo
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Neurosciences
Diego A. Peraza, Pilar Cercos, Pablo Miaja, Yaiza G. Merinero, Laura Lagartera, Paula G. Socuellamos, Carolina Izquierdo Garcia, Sara A. Sanchez, Alejandro Lopez-Hurtado, Mercedes Martin-Martinez, Luis A. Olivos-Ore, Jose R. Naranjo, Antonio R. Artalejo, Marta Gutierrez-Rodriguez, Carmen Valenzuela
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Article
Immunology
Ruth Gil-Prieto, Stefan Walter, Jesus San-Roman-Montero, Patricia Marin-Garcia, Alba Gonzalez-Escalada, Angel Gil-de-Miguel
Article
Multidisciplinary Sciences
Isabel G. Azcarate, Patricia Marin-Garcia, Paloma Abad, Susana Perez-Benavente, Estela Paz-Artal, Pedro A. Reche, Julius N. Fobil, Jose M. Rubio, Amalia Diez, Antonio Puyet, Jose M. Bautista
SCIENTIFIC REPORTS
(2020)
Article
Cell & Tissue Engineering
Lucia Paniagua-Herranz, Aida Menendez-Mendez, Rosa Gomez-Villafuertes, Luis A. Olivos-Ore, Miguel Biscaia, Javier Gualix, Raquel Perez-Sen, Esmerilda G. Delicado, Antonio R. Artalejo, Maria Teresa Miras-Portugal, Felipe Ortega
Article
Anatomy & Morphology
Felipe Ortega, Rosa Gomez-Villafuertes, Maria Benito-Leon, Margaret Martinez de la Torre, Luis A. Olivos-Ore, Marina Arribas-Blazquez, Maria Victoria Gomez-Gaviro, Arturo Azcorra, Manuel Desco, Antonio R. Artalejo, Luis Puelles, Maria Teresa Miras-Portugal
Summary: The purinergic system plays crucial roles in CNS development, with the P2X7 receptor regulating important aspects of neuronal cell biology and neuroprotection. However, difficulties in detecting this receptor pose challenges in studying its functions.
BRAIN STRUCTURE & FUNCTION
(2021)
Article
Biochemistry & Molecular Biology
Paula G. Socuellamos, Luis A. Olivos-Ore, Maria Victoria Barahona, Pilar Cercos, Marta Perez Pascual, Marina Arribas-Blazquez, Jose Ramon Naranjo, Carmen Valenzuela, Marta Gutierrez-Rodriguez, Antonio R. Artalejo
Summary: Neuropathic pain is a chronic form of pain resulting from damage to neural cells. This study evaluated the analgesic effects of IQM-PC332, a new ligand that modulates ion channels, in rats with neuropathic pain. The results showed that IQM-PC332 reduced mechanical sensitivity and modulated ion currents in nerve cells, indicating its potential as a lead compound for novel analgesics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Jesus Garcia Martinez, Sonsoles Garcia Garcia, Stefan Walter, Ruth Gil-Prieto, Daniel Lopez Lacomba, Patricia Marin-Garcia, Angel Gil-de-Miguel, Alba Gonzalez-Escalada
Summary: The study reveals active circulation of Toscana virus in the Autonomous Community of Madrid, Spain. The seroprevalence rate in 2018-19 is lower compared to 2007, potentially due to vector and environmental control measures implemented after the leishmaniasis outbreak.
JOURNAL OF VECTOR BORNE DISEASES
(2022)
Article
Neurosciences
Marina Arribas-Blazquez, Dolores Piniella, Luis A. Olivos-Ore, David Bartolome-Martin, Cristiana Leite, Cecilio Gimenez, Antonio R. Artalejo, Francisco Zafra
Summary: The voltage-sensitive sodium channel Na(V)1.1 is regulated by multiple protein kinases, with AKT1 identified as a novel regulator through direct phosphorylation. AKT1 activation leads to decreased Na+ currents and altered inactivation properties, mimicked by its specific activator SC79 and reverted by a selective inhibitor triciribine. This novel mechanism proposes AKT1 as a key regulator in modulating neuronal excitability under physiological and pathological conditions, including epileptogenesis.
Article
Biochemistry & Molecular Biology
Cristina Martin-Escura, Alicia Medina-Peris, Luke A. Spear, Roberto de la Torre Martinez, Luis A. Olivos-Ore, Maria Victoria Barahona, Sara Gonzalez-Rodriguez, Gregorio Fernandez-Ballester, Asia Fernandez-Carvajal, Antonio R. Artalejo, Antonio Ferrer-Montiel, Rosario Gonzalez-Muniz
Summary: TRPM8 is a key cation channel in pain regulation, with the effectiveness of modulators varying in different animal pain models. RGM8-51, a TRPM8 antagonist, shows promising antinociceptive activity in various pain models, making it a potential pharmacological tool for studying TRPM8-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Paloma Abad, Patricia Marin-Garcia, Marcos Heras, Julius N. Fobil, Alfred G. Hutchful, Amalia Diez, Antonio Puyet, Armando Reyes-Palomares, Isabel G. Azcarate, Jose M. Bautista
Summary: In highly endemic areas, Plasmodium falciparum-specific IgM and IgA can serve as exposure markers, with levels increasing with age. Elevated levels of specific IgM and IgA are associated with microscopic malaria and poor prognosis in children.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Antonio R. Artalejo, Marina Arribas-Blazquez, Maria Victoria Barahona, Celia Llorente-Saez, Luis Alcides Olivos-Ore
Summary: Maria Teresa Miras Portugal dedicated her scientific research to the study of purinergic signaling, primarily using chromaffin cells of the adrenal medulla. She identified diadenosine polyphosphates in these cells and further investigated the purinoceptors involved in adrenal chromaffin cell function. This short review focuses on Maria Teresa's seminal contributions to the understanding of purinoceptors in adrenal chromaffin cells.
PURINERGIC SIGNALLING
(2023)
Article
Biochemical Research Methods
Aida Menendez-Mendez, Lucia Paniagua-Herranz, Luis A. Olivos-Ore, Rosa Gomez-Villafuertes, Raquel Perez-Sen, Esmerilda G. Delicado, Antonio R. Artalejo, Felipe Ortega
Summary: Culturing postnatal cerebellar neural stem cells (NSCs) in low-density cell culture combined with live imaging and single-cell tracking allows monitoring of their behavior and differentiation into neuronal progeny. The protocol maintains the neurogenic nature of NSCs, generating neuronal progeny in proportions that mimic in vivo conditions, and identifies the progeny through post-imaging immunocytochemistry and patch-clamp recordings referenced in Paniagua-Herranz et al. (2020b).
Article
Biochemistry & Molecular Biology
Marina Arribas-Blazquez, Luis Alcides Oliyos-Ore, Maria Victoria Barahona, Aneta Wojnicz, Ricardo De Pascual, Mercedes Sanchez de la Muela, Antonio G. Garcia, Antonio R. Artalejo
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Proceedings Paper
Education & Educational Research
M. Barahona Gomariz, M. Arribas-Blazquez, M. San Andres Larrea, S. Rubio Langre, C. Rodriguez Fernandez, A. Rodriguez Artalejo, L. A. Olivos-Ore
13TH INTERNATIONAL TECHNOLOGY, EDUCATION AND DEVELOPMENT CONFERENCE (INTED2019)
(2019)