Article
Microbiology
Esther Blanco-Paniagua, Laura Alvarez-Fernandez, Andrea Rodriguez-Alonso, Alicia Millan-Garcia, Ana I. Alvarez, Gracia Merino
Summary: Clorsulon is an effective drug for treating helminthic zoonoses and has high broad-spectrum antiparasitic efficacy when used in combination with ivermectin. The safety and efficacy of clorsulon should be studied by considering drug-drug interactions mediated by ABC transporters. This study demonstrates that clorsulon is actively secreted into milk by ABCG2 transporter and its secretion into milk is reduced by coadministration with ivermectin.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Review
Pharmacology & Pharmacy
Valerio Taggi, Mario Riera Romo, Micheline Piquette-Miller, Henriette E. Meyer zu Schwabedissen, Sibylle Neuhoff
Summary: Drug transporters are crucial for maintaining chemical balance and homeostasis in tissues, as well as for the absorption, distribution, and elimination of clinically important drugs. Diseases can alter the expression and function of drug transporters. However, there is limited knowledge on transporter regulation in critical protective barriers, which requires further research.
Article
Chemistry, Analytical
Yanyan Ma, Zhanchen Guo, Chuanwen Fan, Jingran Chen, Shuxin Xu, Zhen Liu
Summary: This study developed aptamers that can target ABCG2 and reverse multidrug resistance, showing great potential in cancer diagnosis and treatment. The aptamers can specifically bind to human colorectal cancer stem cells and effectively reverse drug resistance in liver cancer cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Parasitology
Laura Mate, Mariana Ballent, Candela Canton, Carlos Lanusse, Laura Ceballos, Luis L. Ignacio Alvarez, Juan Pedro Liron
Summary: This study compared the gene expression levels of ABC transporters in sheep nematode Haemonchus contortus isolates with different susceptibility to ivermectin and found that some ABC transporter genes showed minor differences in transcription levels, but could not fully explain ivermectin resistance. Pgp-9 may be one of the most relevant candidates contributing to the multi-genic nature of ivermectin resistance.
VETERINARY PARASITOLOGY
(2022)
Article
Plant Sciences
Georgios Sofianos, Anastasios Samaras, Georgios Karaoglanidis
Summary: Botrytis cinerea is a high-risk pathogen for fungicide resistance development. The study found that MLR/MDR strains are present in Greek populations of strawberries, rootstocks, and tomatoes, with notably high frequencies of resistance. The combination of MLR and MDR mutations leads to even higher levels of fungicide resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Cengiz Gokbulut, Busra Aslan Akyol, Kubra Deliklitas
Summary: A rapid and simple HPLC and modified QuEChERS extraction method was developed and validated for the analysis of anthelmintic MLs residues in cow, sheep, and goat milk. The method is rapid, simple, sensitive, and environmentally friendly, and can be used for multiple residue analyses of anthelmintic MLs in milk for human consumption.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Chiara Bazzocchi, Marco Genchi, Chiara Lucchetti, Alessandra Cafiso, Lavinia Ciuca, John McCall, Laura Helen Kramer, Alice Vismarra
Summary: The combination of doxycycline and macrocyclic lactones (MLs) has shown to have superior adulticidal effects compared to MLs alone. The study found that the interaction between doxycycline/MLs may be due to their interaction with drug efflux transport proteins. The gene expression of these transport proteins showed sex-dependent responses in adult Dirofilaria immitis worms treated with different drugs.
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Adrian J. Wolstenholme, Cedric Neveu
Summary: Glutamate-gated chloride channels are crucial targets for ivermectin and related compounds in parasitic nematodes. Due to variations in gene family members, it is challenging to extrapolate from Caenorhabditis elegans to parasites. Recent advances in localisation data are helping to understand the mode of action of the drugs and the possible combinations of subunits that form their targets in vivo.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shane Denecke, Ivan Rankic, Olympia Driva, Megha Kalsi, Ngoc Bao Hang Luong, Benjamin Buer, Ralf Nauen, Sven Geibel, John Vontas
Summary: By annotating the ABC superfamily across more than 150 arthropod species with good quality protein annotations, specific expansions of ABC transporter families suggesting evolutionary adaptation were highlighted. Functional redundancy was observed in the transporters, with the exception of the highly conserved gene Snu, likely due to its role in cuticular formation. This analysis can be used as a resource for further understanding the ABC superfamily in arthropods.
Article
Biochemistry & Molecular Biology
Jianyi Li, Yuntong Lv, Kunpeng Yan, Fengting Yang, Xuewei Chen, Xiwu Gao, Shuyuan Wen, Hongfei Xu, Yiou Pan, Qingli Shang
Summary: The study revealed that ATP-binding cassette transporters (ABCs) play important roles in cyantraniliprole resistance. Inhibition of ABCs increased the toxicity of cyantraniliprole, while overexpression of ABCs in fruit flies enhanced their tolerance to cyantraniliprole. Suppression of specific ABC transporters increased the sensitivity of cyantraniliprole-resistant strains. These findings provide valuable insights for resistance management strategies.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Microbiology
Lucienne Tritten, Erica J. Burkman, Tobias Clark, Guilherme G. Verocai
Summary: Two novel miRNA candidates secreted by D. immitis larvae have been identified, which could serve as specific diagnostic biomarkers for early detection of infection.
Article
Biochemistry & Molecular Biology
Chang Xu, Xiyun Jin, Songren Wei, Pingping Wang, Meng Luo, Zhaochun Xu, Wenyi Yang, Yideng Cai, Lixing Xiao, Xiaoyu Lin, Hongxin Liu, Rui Cheng, Fenglan Pang, Rui Chen, Xi Su, Ying Hu, Guohua Wang, Qinghua Jiang
Summary: This study presents DeepST, an accurate and universal deep learning framework, which outperforms existing methods in identifying spatial domains. It can dissect spatial domains in cancer tissue at a finer scale and integrate ST data generated from multiple batches or different technologies effectively. These results demonstrate that DeepST is a powerful tool for gaining novel insights from ST studies.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Kaetlyn T. Ryan, Nicolas J. Wheeler, Isaac K. Kamara, Hailey Johnson, Judith E. Humphries, Mostafa Zamanian, John D. Chan
Summary: Macrocyclic lactones are commonly used to treat parasitic roundworm infections, but their activity against parasitic flatworms has not been thoroughly studied. Ivermectin, a prototypical macrocyclic lactone, is known to be ineffective against flatworms. However, other macrocyclic lactones may have antiparasitic activity on flatworms. In this study, an automated imaging assay combined with measurement of lactate levels was used to screen 21 macrocyclic lactones against adult schistosomes. Several macrocyclic lactones were identified to cause paralysis and lack of lactate production in vitro, but further testing in mice did not show a reduction in worm burden.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Ian D. Kerr, Ella Hutchison, Louise Gerard, Shereen M. Aleidi, Ingrid C. Gelissen
Summary: This review provides an update on the function of ABC transporter subfamily members in lipid homeostasis and drug transport, focusing on ABCG1, ABCG2, ABCG4, ABCG5, and ABCG8. Progress in understanding their role in health and disease, recognized transporter substrates, post-translational regulation, microRNA involvement, and structural elements important for transport activity are discussed. Additionally, the impact of lipids such as cholesterol on ABCG2 transport function and a potential model of cholesterol binding sites for ABCG2 are described.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Biochemistry & Molecular Biology
Tongkuo Yuan, Jia Hu, Xiaoming Zhu, Huancai Yin, Jian Yin
Summary: This paper evaluated the role of oxidative stress in the regulation of ABC transporters in human lung cancer cells, and found that it plays an important role in the modulation of ABC transporter expression and activity, as well as chemical toxicity.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)