期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 714, 期 1-3, 页码 332-344出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2013.07.029
关键词
Capsaicin; Allyl isothiocyanate; Substance p; Mast cells; Resiniferatoxin; Pain
资金
- Conselho Nacional de Desenvolvimento Cientifico (CNPq)
- Programa de Apoio aos Nucleos de Excelencia (PRONEX)
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS)
- Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior (CAPES) (Brazil)
- CNPq
- CAPES
Abdominal pain is a frequent symptom of peritoneal cavity irritation, but little is known about the role of the receptors for irritant substances, transient receptor potential vanilloid 1 (TRPV1) and ankyrin 1 (TRPA1), in this painful condition. Thus, we investigated the abdominal nociception caused by peritoneal stimulation with TRPV1 (capsaicin) and TRPA1 (allyl isothiocyanate, AITC) agonists and their mechanisms in rats. The intraperitoneal (i.p.) injection of either capsaicin or AITC (0.03-10 mg/kg) induced short-term (up to 20 min) and dose-dependent abdominal nociception, and also produced c-fos expression in spinal afferents of the dorsal horn. TRPV1 antagonism prevented (94 +/- 4% inhibition) nociception induced by capsaicin but not by AITC. In contrast, the TRPA1 antagonism almost abolished AITC-induced nociception (95 +/- 2% inhibition) without altering the capsaicin response. Moreover, nociception induced by either capsaicin or AITC was reduced by the desensitisation of TRPV1-positive sensory fibres with resiniferatoxin (73 +/- 18 and 76 +/- 15% inhibitions, respectively) and by the NK1 receptor antagonist aprepitant (56 +/- 5 and 53 +/- 8% inhibitions, respectively). Likewise, the i.p. injections of capsaicin or AITC increased the content of substance P in the peritoneal fluid. Nevertheless, neither the mast cell membrane stabiliser cromoglycate, nor the H-1 antagonist promethazine, nor depletion of peritoneal macrophages affected abdominal nociception induced either by capsaicin or AITC. Accordingly, neither capsaicin nor AITC increased the histamine content in the peritoneal fluid or provoked peritoneal mast cell degranulation in vitro. Collectively, our findings suggest that TRPV1 and TRPA1 stimulation in the peritoneum produces abdominal nociception that is mediated by sensory fibres activation. (C) 2013 Elsevier B.V. All rights reserved.
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