期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 690, 期 1-3, 页码 4-12出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2012.05.041
关键词
Methyl lucidone; Microglia; NF-kappa B; Conditioned media; Co-culture; Neurodegeneration
资金
- Jeju National University
Excessive microglial activation-mediated neurotoxicity has been implicated in playing a crucial role in the pathogenesis of stroke and neurodegenerative diseases. Therefore, much attention has been paid to therapeutic strategies aimed at suppressing neurotoxic microglial activation. The microglial regulatory mechanism of methyl lucidone, a cyclopentenedione isolated from the stem bark of Lindera etythrocarpa Makino, was investigated in the present study. Methyl lucidone treatment (0.1-10 mu M) significantly inhibited lipopolysaccharide (LPS, 100 ng/ml, 24 h)-stimulated nitric oxide (NO) production in a dose-dependent manner in both primary cortical microglia and BV-2 cell line. Moreover, it strongly inhibited LPS-stimulated secretion of pro-inflammatory cytokines, such as interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-alpha). Methyl lucidone treatment markedly induced down-regulation of LPS-induced nuclear translocation of nuclear factor kappa B (NF-kappa B) through preventing the degradation of the inhibitory protein I kappa B alpha. In addition, phosphorylation of Akt and mitogen-activated protein kinases (MAPKs) such as extracellular signal-regulated kinase (ERK) and p38 kinases were also suppressed by methyl lucidone. The cell viabilities of HT-22 neurons were significantly attenuated by treatment of the conditioned media containing neurotoxic secretary molecules from LPS-stimulated microglia. However, methyl lucidone significantly blocked neuronal cell death induced by microglial conditioned media. These neuroprotective effects of methyl lucidone were also confirmed in a neuron-microglia co-culture system using EGFP-transfected B35 neuroblastoma cell line. Taken together, these results suggest that methyl lucidone may have a neuroprotective potential via inhibition of neurotoxic microglial activation implicated in neurodegeneration. (C) 2012 Elsevier B.V. All rights reserved.
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