Article
Cardiac & Cardiovascular Systems
Hongguang Sheng, Jun Xiong, Danying Yang
Summary: This study investigated the mechanism of sevoflurane (Sev) in protecting cardiomyocytes from hypoxia/reoxygenation (H/R) injury. The rat cardiomyocyte line H9C2 was subjected to hypoxia followed by reoxygenation to induce H/R injury. The effects of Sev were observed by exposing H9C2 to Sev before hypoxia. Results showed that Sev significantly increased cell viability and decreased the expression of apoptosis-related proteins in the H/R group. Sev also regulated iron homeostasis and ferroptosis by up-regulating ferroportin 1 and mitochondrial ferritin and down-regulating iron regulatory protein 1, divalent metal transporter 1, and transferrin receptor 1 in H/R cells. Moreover, Sev reduced the levels of total Fe, Fe2+, oxidative stress factors, and lipid peroxidation in H/R cells.
CARDIOVASCULAR TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Haiping Ma, Yongjie Li, Tianliang Hou, Jing Li, Long Yang, Hai Guo, Lili Li, Mingxiu Xin, Zhongcheng Gong
Summary: This study demonstrated that sevoflurane post-conditioning (SPostC) has a protective effect on cardiomyocyte hypoxia/reoxygenation injury under low glucose conditions, but this effect is abolished under high glucose conditions. Regulating the HIF-1 alpha/MIF/AMPK signaling pathway can restore the cardioprotective effect of SPostC under hyperglycemia.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Dan Xie, Huifei Deng, Hao Feng
Summary: The study demonstrated that sevoflurane provides better protection for cardiomyocytes with ischemia-reperfusion injury compared to propofol. This is achieved by promoting cell survival and proliferation, while inhibiting apoptosis. The research also revealed the crucial roles of miR-221-5p and ADAM8 in this process.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Haijuan Jing, Chuan Wang, Liang Zhao, Jing Cheng, Pengyu Qin, Hongqi Lin
Summary: This study found that propofol plays a protective role in ameliorating myocardial ischemia-reperfusion injury by modulating the MALAT1/miR-206/ATG3 axis. Propofol pretreatment reduced markers of cardiac injury, suppressed apoptosis, and altered the expression of key genes involved in autophagy, ultimately protecting cardiomyocytes from ischemia-reperfusion injury.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Li Wang, Shaowei Wang, Tong Jia, Xiaojia Sun, Zhen Xing, Hui Liu, Jie Yao, Yanlin Chen
Summary: Myocardial hypoxia/reoxygenation (H/R) injury is a common pathological change in patients with acute myocardial infarction undergoing reperfusion therapy. Dexmedetomidine (DEX) has been found to substantially improve ischemia-mediated cell damage. In this study, we explored the role and mechanism of DEX in ameliorating myocardial H/R injury. Our data showed that DEX treatment notably impeded myocardial apoptosis and inflammation, enhanced cardiomyocyte viability, and improved H/R-mediated myocardial injury by up-regulating Sirt1 through accelerating TET1 and mediating demethylation of the Sirt1 promoter.
Article
Immunology
Chengliang Li, Hong Xin, Yingpeng Shi, Jianjun Mu
Summary: GRX2 plays a protective role in mitigating myocardial ischemia-reperfusion injury by enhancing Nrf2 activation via modulation of the Akt/GSK-3? axis in H/R-induced cardiac injury. The up-regulation of GRX2 enhances Nrf2 signaling, while its knockdown increases sensitivity to apoptosis, oxidative stress, and inflammation in cardiomyocytes subjected to H/R.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucia Longhitano, Alfio Distefano, Paolo Murabito, Marinella Astuto, Anna Nicolosi, Giovanni Buscema, Filippo Sanfilippo, Giuseppe Lazzarino, Angela Maria Amorini, Andrea Bruni, Eugenio Garofalo, Daniele Tibullo, Giovanni Li Volti
Summary: This study evaluated the effects of Dexmedetomidine, Clonidine, and Propofol in a model of hypoxia/reoxygenation injury. The results showed that these drugs can attenuate oxidative stress and inflammation, and restore energy metabolism, with different neuroprotective profiles.
Article
Medicine, Research & Experimental
Nengjing Gong, Xiaohuai Yang, Xiaoyu Li, Yuyu Jiang, Cuifang Gu, Shasha Ma, Qin Gao, Xiangyang Cheng
Summary: MiR-590-3p regulates autophagy and apoptosis in cardiomyocytes after hypoxia/reoxygenation treatment, showing a protective effect and suggesting a potential novel target for the treatment of myocardial ischemic disease.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Ping Liu, Jin Huang, Wanzhen Mei, Xingfang Zeng, Cheng Wang, Chuan Wen, Jing Xu
Summary: The study investigated the protective effect of EGCG on H/R-stimulated cardiomyocytes, showing that EGCG can alleviate cell damage by increasing ATG4C expression. Moreover, overexpression of ATG4C can further enhance the protective effect of EGCG and reduce cell autophagy induced by H/R stimulation.
Article
Medicine, Research & Experimental
Zhongbai Zhang, Xueting Qin, Zhenghui Wang, Yanchun Li, Fei Chen, Rundu Chen, Chuang Li, Wencheng Zhang, Mei Zhang
Summary: OMT pretreatment showed positive effects on H9c2 cardiomyocytes exposed to H/R injury by reducing oxidative stress, decreasing apoptosis, and modulating the PI3K/Akt signaling pathway. This suggests that OMT may be a potential therapeutic candidate for myocardial ischemia/reperfusion injury.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Medicine, Research & Experimental
Feng Zhu, Chi Yuan, Xu Zhang, Zhi Wang, Qiushu Wang, Hegui Wang
Summary: The downregulation of AKAP5 worsened cardiomyocyte remodeling after H/R, possibly by anchoring CaN to form a complex that activates NFATc3 dephosphorylation and expression of hypertrophy-related proteins.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Medicine, Research & Experimental
Chao Cheng, Dong-Ling Xu, Xiao-Bo Liu, Shao-Jie Bi, Juan Zhang
Summary: This study reveals that overexpression of miR-145-5p inhibits H/R-induced cardiomyocyte apoptosis by targeting ROCK1.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Endocrinology & Metabolism
Ying Chen, Bo Chen, Haiwen Hou, Shanshan Xu
Summary: Trimetazidine protects cardiomyocytes from ischemia/reperfusion injury by modulating the miR-125b-5p/ATG4D axis. Hypoxia/reoxygenation reduces the expression of miR-125b-5p and cell viability, while increasing apoptosis and the protein levels related to autophagy and apoptosis (p < 0.01). These effects are reversed by TMZ and are largely diminished by miR-125b-5p inhibitor.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Biotechnology & Applied Microbiology
Chenyu Liang, Yang Liu, Huifeng Xu, Junling Huang, Yi Shen, Faxiu Chen, Ming Luo
Summary: The enriched miR-100-5p in hucMSC-exo suppressed the expression of FOXO3 to inhibit NLRP3 inflammasome activation, reduce cytokine release, and protect cardiomyocytes from H/R-induced pyroptosis and injury.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Weiguo Chen, Jianbang Wang, Xihui Wang, Pan Chang, Meng Liang
Summary: Knockdown of HIF1A downregulates ANGPTL2 to alleviate H/R injury in HG-induced H9c2 cells by activating the Nrf2/HO-1 pathway.
Article
Biochemistry & Molecular Biology
Yeongun Lee, So Hee Dho, Jiyeon Lee, Ji-Hyun Hwang, Minjung Kim, Won-Young Choi, Jin-Young Lee, Jongwon Lee, Woochul Chang, Min Young Lee, Jungmin Choi, Tae-You Kim, Lark Kyun Kim
Summary: A study has identified gene-specific alterations associated with worse outcomes in colorectal cancer patients through detecting patterns of DNA modification. The results could provide clinicians with an additional diagnostic tool and suggest the potential utility of performing such methylation profiling in other cancer types.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Plant Sciences
Gang Wu, Shingo Miyauchi, Emmanuelle Morin, Alan Kuo, Elodie Drula, Torda Varga, Annegret Kohler, Bang Feng, Yang Cao, Anna Lipzen, Christopher Daum, Hope Hundley, Jasmyn Pangilinan, Jenifer Johnson, Kerrie Barry, Kurt LaButti, Vivian Ng, Steven Ahrendt, Byoungnam Min, In-Geol Choi, Hongjae Park, Jonathan M. Plett, Jon Magnuson, Joseph W. Spatafora, Laszlo G. Nagy, Bernard Henrissat, Igor V. Grigoriev, Zhu-Liang Yang, Jianping Xu, Francis M. Martin
Summary: This study compared the genomes of 21 ectomycorrhizal species within Boletales with their saprotrophic brown-rot relatives, revealing that symbiotic boletes have become functionally diverse over their evolutionary history with reduced plant cell-wall-degrading enzymes but distinct sets of enzymes. Additionally, the study showed that transposable element proliferation contributed to the higher evolutionary rate of certain genes, while the loss of secreted CAZymes was related to DNA decay rather than TE activity. These findings provide new insights into the mechanisms influencing the evolutionary diversification of symbiotic boletes.
Correction
Food Science & Technology
Hye Won Kim, Byeong Hyeok Park, Hongjae Park, Beom Chang Baek, In-Geol Choi, Min Suk Rhee
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
H. W. Kim, B. H. Park, H. Park, B. C. Baek, I. G. Choi, M. S. Rhee
Summary: Low-shear modeled microgravity (LSMMG) affects the growth, biofilm formation, and pathogenicity of Escherichia coli O157:H7. Transcriptomic analysis reveals upregulation of genes involved in nutrient and energy metabolism, glycogen biosynthesis, and secretion pathway, while downregulation of genes related to host cell contact secretion. LSMMG also stimulates the transcriptional upregulation of Shiga toxin 1 and toxin HokB.
FOOD RESEARCH INTERNATIONAL
(2022)
Correction
Genetics & Heredity
Duleepa Pathiraja, June Wee, Kijong Cho, In-Geol Choi
ENVIRONMENTAL MICROBIOME
(2022)
Article
Genetics & Heredity
Duleepa Pathiraja, June Wee, Kijong Cho, In-Geol Choi
Summary: This study determined the microbiota composition of laboratory-maintained Collembola and found that it is influenced by the soil microbial community. The results demonstrate that the bacterial community of adult Collembola can serve as an indicator to evaluate the soil ecosystem.
ENVIRONMENTAL MICROBIOME
(2022)
Article
Microbiology
Chul-Hwan Kim, Yeonjae Yoo, Dongjun Kim, Jong Seong Khim, Duleepa Pathiraja, Bogun Kim, In-Geol Choi, Jae-Jin Kim
Summary: A new species of bacterium, Aurantiacibacter sediminis JGD-13T, was isolated from a tidal flat in South Korea. It has distinctive characteristics and shows similarities with related strains.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Food Science & Technology
Duleepa Pathiraja, Junghwan Cho, Junsung Kim, In-Geol Choi
Summary: This study applied eDNA metabarcoding to analyze the DNA signatures of bacteria, plants, and honey bees in 48 commercial honey products from different geographical origins. The results showed that the bacterial DNA composition varied among samples from different regions, while the floral DNA signatures could identify the source of the honey. Additionally, the DNA sequences from honey bees could trace the geographical origin of the honey.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Microbiology
Yeonjae Yoo, Hanbyul Lee, Jong Seong Khim, Xiaoyue Xu, Bogun Kim, In-Geol Choi, Jae-Jin Kim
Summary: A novel halotolerant bacterium, Halomonas getboli, was isolated from saltern sediment in Yongyu- do, Republic of Korea. It showed high salt and pH tolerance.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Duleepa Pathiraja, Junghwan Cho, Peter Stougaard, In-Geol Choi
Summary: Researchers developed an enzymatic process to obtain β-neocarrabiose and 3,6-anhydro-D-galactose from kappa-carrageenan, which are important bioactive substances with potential applications in functional foods and drugs.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Engineering, Electrical & Electronic
Heon Ick Park, Tae Jin Cho, In-Geol Choi, Min Suk Rhee, Youngsu Cha
Summary: In this study, smart finger devices (SFDs) were proposed for object classification using unimodal temperature sensors. Each SFD consisted of a module with a flexible thermoelectric device (TED) and a resistance temperature detector (RTD) sensor embedded in a silicone finger cot on a robot gripper. The Peltier heat stored on the TED was transferred to the object when gripped by the robot. The RTD sensor data, processed through a one-dimensional convolutional neural network (1D-CNN), accurately distinguished materials with similar thermal conductivities. The designed classifier identified ten selected objects using the preprocessed sensor data, enabling real-time recognition with unimodal temperature sensors.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Biochemical Research Methods
Duleepa Pathiraja, Byeonghyeok Park, Bogun Kim, Peter Stougaard, In-Geol Choi
Summary: Marine red algal biomass is a potential feedstock for sustainable production of value-added chemicals. In this study, the marine heterotrophic bacterium Pseudoalteromonas atlantica T6c was used as a metabolic chassis to produce valuable chemicals from low-cost red algal galactans or industrial waste agaropectin.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Bogun Kim, Ziayo Meng, Xiaoyue Xu, Seungwoo Baek, Duleepa Pathiraja, In-Geol Choi, Sejong Oh
Summary: Lactic acid bacteria (LAB) have important applications in milk fermentation, industrial lactic acid fermentation, and health and medicine. Limosilactobacillus fermentum, isolated from fermented dairy and food products, is considered safe and has beneficial effects on the intestinal microbiota, host immune system, and feed digestibility.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)
Article
Agronomy
Bogun Kim, Byoungnam Min, Jae-Gu Han, Hongjae Park, Seungwoo Baek, Subin Jeong, In-Geol Choi
Summary: This study presents the draft genome sequence of Wolfiporia cocos strain KMCC03342 and predicts 19 terpene biosynthesis gene clusters. This provides insights into the biosynthetic pathways of the active ingredients of W. cocos.
Review
Biochemistry & Molecular Biology
Byeong-Wook Song, Sekyung Oh, Woochul Chang
Summary: Regenerative medicine aims to restore damaged cell or tissue function through regeneration, repair, or replacement. Stem cells are crucial for regenerative medicine, as they can be used to promote responses to diseases and dysfunctions. Stem cell-derived extracellular vesicles (EVs) are a promising source that can enrich microRNAs (miRNAs) for disease therapy, overcoming current limitations of cell therapy agents.
