Article
Pharmacology & Pharmacy
Katarzyna Dobrowolska, Andrzej Emeryk, Kamil Janeczek, Radoslaw Krzosa, Michal Pirozynski, Tomasz R. Sosnowski
Summary: The physicochemical properties of micronized steroid suspensions have a weak influence on droplet size distribution and aerosol deposition, but affect the conversion of the drug to inhalable aerosol. The maximum inhaled dose of budesonide is below 80-90% of the label dose, depending on the formulation. This study highlights the sensitivity of nebulization in vibrating mesh nebulizers to minor dissimilarities among generic pharmaceuticals and discusses their potential clinical relevance.
Article
Pharmacology & Pharmacy
Cruz-Zazueta Isabel, Arias-Duran Luis, Estrada-Soto Samuel, Pinon-Segundo Elizabeth, Herrera-Ruiz Dea, Alcala-Alcala Sergio
Summary: In this study, FEL-PLGA-PVM/MA composites were prepared and characterized for their physical and rheological properties. The composites showed sustained drug release and mucoadhesive properties. Nebulization of the composites in hypertensive rats led to a significant decrease in blood pressure, indicating the promising potential of these novel composites for treating systemic diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Physical
David M. Klein, Albert Poortinga, Frank M. Verhoeven, Daniel Bonn, Sylvestre Bonnet, Cees J. M. van Rijn
Summary: The study found that vibrating mesh nebulizers cause model liposomes to release over ten times more encapsulated material than continuous jet nebulizers. This highlights the importance of using a gentle nebulization technology when administering shear-sensitive lipid nanoparticle drugs to the lungs.
Article
Medicine, General & Internal
Haijia Hou, Dongyang Xu, Bing Dai, Hongwen Zhao, Wei Wang, Jian Kang, Wei Tan
Summary: This study investigated the effect of different types and positions of nebulizers on aerosol delivery efficiency during adult mechanical ventilation. The results showed that the type and position of the nebulizer had an impact on aerosol delivery efficiency, but there were no significant differences between different ventilators.
FRONTIERS IN MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Luciana Alcoforado, Dulciane Nunes Paiva, Arzu Ari, Jacqueline de Melo Barcelar, Simone Cristina Soares Brandao, James B. Fink, Armele Dornelas de Andrade
Summary: This study compared the pulmonary deposition of a jet nebulizer (JN) and a mesh nebulizer (MN) with and without a valved holding chamber (VHC) in a randomized cross-over trial. The results showed that using a VHC significantly increased pulmonary deposition for the JN, while the MN with VHC had the highest pulmonary deposition. However, the addition of a VHC did not substantially improve the efficiency of aerosol lung deposition for the JN alone.
Article
Pharmacology & Pharmacy
Matthias Otto, Yannik Kropp, Evelyn Jaeger, Michael Neumaier, Manfred Thiel, Michael Quintel, Charalambos Tsagogiorgas
Summary: Mechanically ventilated patients with acute respiratory distress syndrome (ARDS) often receive aerosolized iloprost, and prolonged inhalation may improve its clinical effectiveness. The use of inspiration-synchronized vibrating mesh nebulizers (VMNsyn) is a possible solution, as they achieve high drug deposition rates and longer nebulization times. In an in vitro model, the VMNsyn and a continuous vibrating mesh nebulizer (VMNcont) were compared in different ventilation modes, with the VMNsyn showing comparable deposition rates to the VMNcont during volume-controlled ventilation, but 10.9% lower during pressure-controlled continuous mandatory ventilation. The nebulization time was significantly longer with the VMNsyn during both ventilation modes. Therefore, inspiration-synchronized nebulization during volume-controlled ventilation mode seems to be the most suitable for prolonged inhalative iloprost administration in mechanically ventilated patients.
Article
Instruments & Instrumentation
Annalisa Bianchera, Viviana Vilardo, Roberta Giaccari, Annalisa Michielon, Gianluca Bazzoli, Francesca Buttini, Marina Aiello, Alfredo Chetta, Stefano Bruno, Ruggero Bettini
Summary: Two nebulizers, a jet and a mesh vibrating system, were compared in terms of performance and effectiveness for delivering alpha-1 antitrypsin (AAT) to the lungs. The mesh nebulizer demonstrated higher efficiency in drug delivery, with preserved protein activity and conformation. Nebulization of AAT is a suitable administration strategy for AATD patients, either as a support therapy or for preventive purposes.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Environmental Sciences
Yu Liu, Xiaole Chen, Zhengqi Li, Huizhen Yang, Jianwei Wang
Summary: This study investigates the impact of different air inlet structures on the deposition fraction of droplets generated by a vibrating mesh nebulizer (VMN). The results indicate that optimizing the VMN's air inlet structure significantly enhances droplet delivery efficiency, with the multiple-orifice inlet structure outperforming other designs.
Review
Pharmacology & Pharmacy
Li Ding, Jieliang Wang, Shihao Cai, Hugh Smyth, Zhengrong Cui
Summary: Certain pulmonary diseases are often accompanied by respiratory tract infections, which may lead to chronic biofilm-based infections that are challenging to treat with antimicrobial agents. Therefore, effective agents and delivery carriers must be developed to target biofilms in the lung. This review focuses on inhalable agents to treat biofilms, discussing existing options as well as promising novel agents and delivery devices.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Maria Cabrera, Deborah Le Pennec, Sandrine Le Guellec, Jeoffrey Pardessus, Stephan Ehrmann, Ronan Macloughlin, Nathalie Heuze-Vourc'h, Laurent Vecellio
Summary: This study evaluated the influence of mesh nebulizer parameters on lung deposition in macaques using a 3D printed macaque model of the upper respiratory tract. The positioning of the nebulizer on the mask, aerosol particle size, and aerosol flow rate were found to affect lung deposition. Placing the nebulizer in the upper part of the mask and in a proximal position to the macaque, as well as using smaller aerosol sizes and lower flow rates, improved lung delivery. However, the variability of lung deposition in macaques remained high.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Cristina Lalmolda, Pablo Florez, Carles Grimau, Roberto Larrosa, Marta Corral, Javier Sayas, Manel Lujan
Summary: The introduction of external gas in the NIV circuit can lead to asynchronies in both bench and patient studies, with autotriggering being the most common issue. Variations were observed based on the ventilator model and gas source used. Monitoring the patient at the initiation of treatment is recommended due to the variable impact of external gas on ventilator triggers.
SCIENTIFIC REPORTS
(2021)
Article
Respiratory System
Wei Tan, Bing Dai, Chang-ling Lu, Hai-jia Hou, Hong-wen Zhao, Wei Wang, Jian Kang
Summary: Different interfaces, inner volumes, intentional leaks, and leak port locations have significant effects on aerosol delivery during noninvasive positive pressure ventilation. Additionally, adding a 15 cm tube between the nebulizer and the mask can significantly increase aerosol delivery.
JOURNAL OF AEROSOL MEDICINE AND PULMONARY DRUG DELIVERY
(2021)
Article
Medicine, General & Internal
Basma M. E. Mohamed, Randa Salah Eldin, Abeer Salah Eldin, Mohamed E. A. Abdelrahim, Raghda R. S. Hussein
Summary: This study aimed to assess the influence of smoking on the lung deposition of aerosol delivered to exacerbated COPD subjects. The results showed that smoking reduced the lung deposition of inhaled salbutamol but increased systemic absorption. Further studies are needed to consider these effects when prescribing inhaled medication to COPD smokers, especially exacerbated patients.
INTERNATIONAL JOURNAL OF CLINICAL PRACTICE
(2021)
Article
Biophysics
Jana Szabova, Ondrej Misik, Martina Havlikova, Frantisek Lizal, Filip Mravec
Summary: This study investigated the effects of cholesterol, phosphatidic acid, and polyethylene glycol on the stability and properties of liposomes. Results showed that cholesterol increased membrane fluidity for liposome stabilization, while higher amounts of PEG were needed for successful steric stabilization. The nebulization process slightly influenced immobilized water and the rigidity of the inner part of the membrane for the two lowest concentrations of PEG.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Sadegh Niazi, Lisa K. Philp, Kirsten Spann, Graham R. Johnson
Summary: Laboratory-generated bioaerosols are widely used in studying virus transmission. In this study, we compared the performance of different nebulizers in aerosol production and virus survival. The results showed that while the hydraulic spray atomizer had the least impact on influenza viruses, the vibrating mesh nebulizer had the least impact on human rhinovirus.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Allergy
Mathias Hochgerner, Eva M. Sturm, Diana Schnoegl, Grazyna Kwapiszewska, Horst Olschewski, Leigh M. Marsh
Summary: High-altitude therapy may have therapeutic effects on allergic asthma by reducing mucosal hypersecretion and airway hyper-responsiveness. This therapy suppresses the adaptive immune response to allergens in both mouse and human immune cells. Hypoxia interferes with antigen-presenting cell/T-cell crosstalk, leading to an unresponsive phenotype in antigen-presenting cells.
Article
Critical Care Medicine
Lars Harbaum, Christopher J. Rhodes, John Wharton, Allan Lawrie, Jason H. Karnes, Ankit A. Desai, William C. Nichols, Marc Humbert, David Montani, Barbara Girerd, Olivier Sitbon, Mario Boehm, Tatyana Novoyatleva, Ralph T. Schermuly, H. Ardeschir Ghofrani, Mark Toshner, David G. Kiely, Luke S. Howard, Emilia M. Swietlik, Stefan Graf, Maik Pietzner, Nicholas W. Morrell, Martin R. Wilkins
Summary: Integrated analysis of the plasma proteome and genome implicates two secreted matrix-binding proteins, netrin-4 and thrombospondin-2, in the pathobiology of PAH.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Respiratory System
Anna Birnhuber, Katharina Jandl, Valentina Biasin, Elisabeth Fliesser, Francesco Valzano, Leigh M. Marsh, Christina Krolczik, Andrea Olschewski, Jochen Wilhelm, Wolfgang Toller, Akos Heinemann, Horst Olschewski, Malgorzata Wygrecka, Grazyna Kwapiszewska
Summary: This study shows that pirfenidone may have limited effectiveness in treating SSc-ILD, and caution should be taken in patients with high T-helper type 2 inflammation to avoid unwanted interactions.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Pharmacology & Pharmacy
Carolina Alva, Ivan Vidakovic, Barbara Lorber, Anna-Laurence Schachner-Nedherer, Manuel Zettl, Johannes Khinast, Ruth Prassl, Wen-Kai Hsiao
Summary: This study aims to verify the suitability of inkjet printing technology for liposomes. The results show that the concentration and size distribution of liposomes are largely unaffected by the shear forces during the jetting process.
JOURNAL OF PHARMACEUTICAL INNOVATION
(2023)
Article
Medicine, Research & Experimental
Manu Manjunath Kanti, Isabelle Striessnig-Bina, Beatrix Irene Wieser, Silvia Schauer, Gerd Leitinger, Thomas O. Eichmann, Martina Schweiger, Margit Winkler, Elke Winter, Andrea Lana, Iris Kufferath, Leigh Matthew Marsh, Grazyna Kwapiszewska, Rudolf Zechner, Gerald Hoefler, Paul Willibald Vesely
Summary: The energy metabolism of bronchiolar club cells plays a crucial role in the repair of lung airway damage and is essential for lung health.
Article
Physics, Applied
Marko Simic, Doris Auer, Christian Neuper, Nikola Simic, Gerhard Prossliner, Ruth Prassl, Christian Hill, Ulrich Hohenester
Summary: This article proposes and demonstrates a scheme for optical nanoparticle characterization, which achieves real-time optical counting with single-particle sensitivity and high throughput. By monitoring the trajectories and velocity changes of each tracked particle, detailed information about the particle size distribution can be obtained. The experimental results show that this method is suitable for various applications.
PHYSICAL REVIEW APPLIED
(2022)
Letter
Critical Care Medicine
Katharina Jandl, Leigh M. Marsh, Ayse Ceren Mutgan, Slaven Crnkovic, Francesco Valzano, Diana Zabini, Julia Hoffmann, Vasile Foris, Elisabeth Gschwandtner, Walter Klepetko, Helmut Prosch, Holger Flick, Luka Brcic, Izidor Kern, Akos Heinemann, Horst Olschewski, Gabor Kovacs, Grazyna Kwapiszewska
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Peripheral Vascular Disease
Slaven Crnkovic, Sonja Rittchen, Katharina Jandl, Juergen Gindlhuber, Diana Zabini, Ayse Ceren Mutgan, Francesco Valzano, Panja M. Boehm, Konrad Hoetzenecker, Wolfgang Toller, Christine Veith, Akos Heinemann, Ralph T. Schermuly, Andrea Olschewski, Leigh M. Marsh, Grazyna Kwapiszewska
Summary: Through this study, it was found that ephrin-B2 and EphB4 expression is preserved in smooth muscle and endothelial cells of remodeled pulmonary arteries. Lack of ephrin-B2 gene attenuates pulmonary vascular remodeling and right ventricular hypertrophy in chronic hypoxia-induced pulmonary hypertension, while inhibition of EphB4 signaling reduces pulmonary vascular remodeling without affecting right ventricular systolic pressure. In conclusion, ephrin-B2 and EphB4 play crucial roles in pulmonary vascular remodeling.
Article
Chemistry, Multidisciplinary
Atida Selmani, Ramona Jeitler, Michael Auinger, Carolin Tetyczka, Peter Banzer, Brian Kantor, Gerd Leitinger, Eva Roblegg
Summary: Biocompatible gold nanoparticles have been utilized in wound healing due to their radical scavenging activity. An acidic microenvironment is also found to promote wound healing through cell proliferation and inhibit bacterial growth. Combining these two approaches shows promising results for wound healing.
Article
Allergy
Fahd Alhamdan, Timm Greulich, Christian Daviaud, Leigh M. Marsh, Frauke Pedersen, Clemens Thoelken, Petra Ina Pfefferle, Thomas Bahmer, Daniel P. Potaczek, Joerg Tost, Holger Garn
Summary: This study aimed to use EV miRNA signatures to uncover the molecular mechanisms of obesity-associated low type-2 asthma. EV miRNA profiles were able to clearly distinguish between obese low type-2 asthma, non-obese low type-2 asthma, and healthy control groups. Pathway analysis revealed inflammatory cytokines and metabolic factors signaling pathways to be specifically associated with obesity-associated low type-2 asthma. Additionally, miR-17-92 and miR-106a-363 clusters were significantly enriched in obesity-associated low type-2 asthma and exhibited correlations with key laboratory and lung function parameters.
Article
Pharmacology & Pharmacy
Anna-Laurence Schachner-Nedherer, Julia Fuchs, Ivan Vidakovic, Oliver Hoeller, Gebhard Schratter, Gunter Almer, Eleonore Froehlich, Andreas Zimmer, Martin Wabitsch, Karin Kornmueller, Ruth Prassl
Summary: Obesity and type 2 diabetes are significant health burdens without effective treatment options. This study explores the use of PEG-LNPs to deliver anti-adipogenic miRNAs (miR26a and miR27a) in order to regulate gene expressions and balance adipose tissue metabolic functions. The findings demonstrate that upregulating miR27a inhibits adipogenesis in preadipocytes, while transfecting miR26a induces white adipocyte browning. Selective delivery of miRNAs to adipocytes through PEG-LNPs may offer new possibilities for treating obesity and related metabolic diseases.
Article
Medicine, Research & Experimental
Izabela Borek, Anna Birnhuber, Norbert F. Voelkel, Leigh M. Marsh, Grazyna Kwapiszewska
Summary: The role of pulmonary vasculature in the development and progression of acute and chronic lung diseases, such as ARDS, PF, and COPD, has been frequently overlooked. However, there is increasing evidence that the vascular endothelium plays an active role in these diseases. This review outlines the current knowledge and future research directions regarding the pulmonary vasculature and hopes to facilitate the development of mechanism-based therapies.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Respiratory System
Andreas Horner, Horst Olschewski, Sylvia Hartl, Arschang Valipour, Georg-Christian Funk, Michael Studnicka, Monika Merkle, Bernhard Kaiser, Eva Maria Wallner, Stephan Brecht, Bernd Lamprecht
Summary: In stable COPD outpatients in Austria, nearly half of the patients reported symptoms of depression. These symptoms were associated with lower levels of self-reported physical activity, more pain, and respiratory symptoms, with particularly strong associations in SGRQ-C.
INTERNATIONAL JOURNAL OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
(2023)