Article
Chemistry, Multidisciplinary
Wen Yang, Lin Wang, Mulin Fang, Vinit Sheth, Yushan Zhang, Alyssa M. Holden, Nathan D. Donahue, Dixy E. Green, Alex N. Frickenstein, Evan M. Mettenbrink, Tyler A. Schwemley, Emmy R. Francek, Majood Haddad, Md Nazir Hossen, Shirsha Mukherjee, Si Wu, Paul L. DeAngelis, Stefan Wilhelm
Summary: In this study, we explored the use of self-polysaccharide heparosan (HEP) as a surface modification for nanoparticles. Our findings showed that HEP-coating reduced the protein corona formation and increased the uptake of nanoparticles by certain innate immune cells without causing cytotoxicity.
Review
Oncology
Seyedeh Alia Moosavian, Vanessa Bianconi, Matteo Pirro, Amirhossein Sahebkar
Summary: This review highlights the challenges in translating liposomal delivery systems from preclinical studies to clinical application in cancer therapy. The gap between experimental results and practical use of liposomes is a major concern that needs to be addressed in order to improve the efficacy of this drug delivery system. Various potential approaches to overcome the barriers in clinical application of liposomal formulations are also discussed.
SEMINARS IN CANCER BIOLOGY
(2021)
Review
Medicine, Research & Experimental
Mingyue Cui, Christian Wiraja, Sharon Wan Ting Chew, Chenjie Xu
Summary: Topical drug delivery has advantages over other routes, but the stratum corneum poses a challenge. Nanotechnology offers opportunities to overcome this challenge with nanocarriers like liposomes and nanoparticles. These formulations show potential in treating skin disorders, with further developments needed.
MOLECULAR PHARMACEUTICS
(2021)
Review
Chemistry, Multidisciplinary
Saeid Moghassemi, Arezoo Dadashzadeh, Ricardo Bentes Azevedo, Olivier Feron, Christiani A. Amorim
Summary: Photodynamic therapy (PDT) combines photochemistry and photophysics sciences for cancer therapy, showing significant potential. Despite advantages and progress, common photosensitizing agents have limitations, which can be addressed by using liposomes as carriers for PS.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Materials Science, Multidisciplinary
Ipshita Menon, Maryam Zaroudi, Yuanzhe Zhang, Elizabeth Aisenbrey, Liwei Hui
Summary: Lipid nanoparticles have promising applications as non-viral vectors for targeted drug and gene delivery. This review focuses on the techniques to fabricate active targeting lipid nanoparticles, including one-pot assembly and post-insertion functionalization. The advantages, restrictions, and challenges in formulation screening and clinical translation are discussed.
MATERIALS TODAY ADVANCES
(2022)
Article
Chemistry, Multidisciplinary
Maximilian Regenold, Kan Kaneko, Xuehan Wang, H. Benson Peng, James C. Evans, Pauric Bannigan, Christine Allen
Summary: Triggered drug delivery strategies, such as thermosensitive liposomes, have shown improved targetability for chemotherapeutics. However, most studies have focused on doxorubicin encapsulation, while other drugs could also benefit from this targeted delivery approach.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Pharmacology & Pharmacy
Devaraj Ezhilarasan, Karthik Shree Harini
Summary: Kupffer cells (KCs) are resident macrophages of the liver that play a crucial role in the innate immunity. Strategies such as PEGylation, transient clearance of KCs, and surface modification of nanoparticles have shown promise in reducing liver accumulation of nanoparticles. However, the safety of KCs depletion strategies and their impact on the immune system need further investigation.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Nooshin Tasharrofi, Mohammad Nourozi, Abdolrazagh Marzban
Summary: The eye is a highly specialized organ, and delivering drugs to it is challenging. The development of novel drug delivery systems, like nanoparticles, offers potential solutions. This review focuses on liposomes as effective carriers and their impact on ocular drug delivery.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ruoyu Shen, Hong Yuan
Summary: Poly(ethylene glycol) (PEG) is widely used in the biomedical field as a gold standard. PEGylation, the conjugation of PEG to various substances, is a common method to improve drug delivery and pharmacokinetics. It has been extensively studied and proven effective in prolonging in vivo circulation time and reducing immunogenicity. This article discusses the background, advantages, applications, limitations, and possible alternatives of PEGylation in drug delivery.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Dermatology
Soraya Babaie, Mohammad Charkhpour, Maryam Kouhsoltani, Hamed Hamishehkar, Ana Claudia Paiva-Santos
Summary: By using lipid-based nanovesicles, buprenorphine and bupivacaine can be co-delivered to effectively target pain receptors concentrated in the skin, resulting in potent dermal anesthesia.
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Pharmacology & Pharmacy
Domenico Lombardo, Mikhail A. Kiselev
Summary: Liposomes are nano-sized vesicles commonly used in nanomedicine and biomedical applications. They have high biocompatibility and can enhance drug delivery by reducing side effects and toxicity. However, liposomes are sensitive to mechanical and chemical actions, leading to limited colloidal stability and reduced cargo molecule entrapment.
Article
Chemistry, Multidisciplinary
Shanbo Sun, Wei Lv, Shengnan Li, Qi Zhang, Weichong He, Zhiyi Min, Chuanhui Teng, Yuqin Chen, Linfeng Liu, Jiaqing Yin, Baoli Zhu, Ming Xu, Dongwei Dai, Hongliang Xin
Summary: A smart multifunctional delivery system has been developed to regulate immune disorders in ischemic brain. The system can effectively suppress the release of NETs and inhibit the cGAS-STING pathway, thereby reducing ischemic and reperfusion injury.
Review
Polymer Science
Khusbu Dwivedi, Ashok Kumar Mandal, Obaid Afzal, Abdulmalik Saleh Alfawaz Altamimi, Ankit Sahoo, Manal A. Alossaimi, Waleed H. Almalki, Abdulaziz Alzahrani, Md. Abul Barkat, Tahani M. Almeleebia, Shehla Nasar Mir Najib Ullah, Mahfoozur Rahman
Summary: Flavonoids are natural polyphenolic compounds found in various sources such as vegetables, fruits, flowers, seeds, wine, tea, nuts, propolis, and honey. They have diverse pharmacological activities including prevention of coronary heart disease, antioxidative, hepatoprotective, anti-inflammatory, free-radical scavenging, anticancer, and anti-atherosclerotic activities. Flavonoids also exhibit potent antimicrobial activity against a wide range of pathogenic microorganisms, and some can reverse antibiotic resistance and enhance the efficacy of existing antibiotics. However, their poor solubility in water limits their oral bioavailability, making the integration of flavonoids into nanomedicine a promising strategy for efficient delivery.
Article
Engineering, Biomedical
William Marcondes Facchinatto, Joana Galante, Leticia Mesquita, Daniella Souza Silva, Danilo Martins dos Santos, Tiago Bueno Moraes, Sergio Paulo Campana-Filho, Luiz Alberto Colnago, Bruno Sarmento, Jose das Neves
Summary: This study explored the use of novel nanoparticles as carriers for clotrimazole, demonstrating good drug release properties and improved drug permeability in cell monolayer models.
ACTA BIOMATERIALIA
(2021)
Review
Chemistry, Multidisciplinary
Qi Jiang, Shufang Zhang
Summary: Osteoarthritis (OA) is a common age-related degenerative disease that poses economic and societal burdens, especially with the increasing aging population. Stimulus-responsive nanoplatforms offer improved therapeutic strategies for OA, with potential benefits such as enhanced control, longer retention time, higher loading rates, and increased sensitivity. This review summarizes the advanced application of stimulus-responsive drug delivery nanoplatforms, categorized by endogenous and exogenous stimuli, and discusses the opportunities, restrictions, and limitations of these systems.
Article
Ophthalmology
Anne Hedengran, Josefine Clement Freiberg, Pernille May Hansen, Jette Jacobsen, Susan Weng Larsen, Stine Harloff-Helleberg, Kristine Freude, Gerard Boix-Lemonche, Goran Petrovski, Steffen Heegaard, Miriam Kolko
Summary: BAK-preserved travoprost caused GC loss, indicating that PQ preservation may be preferable for glaucoma treatment. Furthermore, the physicochemical properties of branded and generic travoprost eye drops cannot be assumed to be identical.
ACTA OPHTHALMOLOGICA
(2022)
Article
Pharmacology & Pharmacy
Janni Stovring Mortensen, Lasse Saaby, Stine Harloff-Helleberg, Hanne Morck Nielsen
Summary: This study investigated the effect of isolation procedures and storage conditions on the properties of porcine intestinal mucus. The findings suggest that different isolation procedures and storage conditions do not significantly impact the critical properties of isolated mucus, and stored mucus samples can be considered good alternatives to fresh mucus in studies.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
You Xu, Laure Harinck, Abhijeet G. Lokras, Per Gerde, Ewa Selg, Carl-Olof Sjoberg, Henrik Franzyk, Aneesh Thakur, Camilla Foged
Summary: The study demonstrates that a mixture of stabilizing excipients trehalose and dextran with the shell-forming dispersion enhancer leucine can stabilize TNF-alpha siRNA-loaded LPNs and result in DPI formulations with high aerosol performance. At optimal ratios, the formulations display suitable aerosol properties for inhalation and homogeneous deep lung deposition.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Abhijeet Lokras, Akash Chakravarty, Thomas Rades, Dennis Christensen, Henrik Franzyk, Aneesh Thakur, Camilla Foged
Summary: In this study, a new analytical method was developed for the simultaneous quantification of single-stranded and double-stranded RNA drug cargos. The extraction and separation of RNA cargos from nanoparticle carriers were achieved using ion-pair reversed-phase high-performance liquid chromatography combined with UV detection. The methods were sensitive, linear, and accurate, allowing for convenient quantification of multiple RNA cargos loaded into nanoparticle-based delivery systems.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
J. S. Mortensen, S. S. -R Bohr, S. Harloff-Helleberg, N. S. Hatzakis, L. Saaby, H. M. Nielsen
Summary: This study investigated the interaction between the permeation enhancer SNAC and gastrointestinal mucus barrier. The results showed that SNAC altered the physical and barrier properties of intestinal mucus, but did not affect gastric mucus. The study highlights the importance of choosing the appropriate mucus model for such studies.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Medicine, Research & Experimental
Olivia Amanda Oest Mullertz, Peter Andersen, Dennis Christensen, Camilla Foged, Aneesh Thakur
Summary: The mucosal surfaces of the lungs are a major site for entry of airborne pathogens and pulmonary vaccination can induce protective mucosal immunity. Researchers found that altering the surface charge of liposomes can enhance specific T-cell responses, but the impact on mucosal immunity remains unclear.
MOLECULAR PHARMACEUTICS
(2022)
Article
Nanoscience & Nanotechnology
Matteo Tollemeto, Zheng Huang, Jorn B. Christensen, Hanne Morck Nielsen, Stine Ronholt
Summary: Biological drugs are increasingly important for the treatment of diseases such as diabetes, cancer, and obesity. This project explores the potential of using mucoadhesive delivery systems based on dendron-coated mesoporous silica nanoparticles for oral delivery of biological drugs. The study focuses on the interaction between dendrons and mucin, and successfully conjugates dendrons onto mesoporous silica nanoparticles. The interaction between the nanoparticles and mucin or intestinal mucus is investigated, providing knowledge for the development of mucoadhesive nanoformulations for oral delivery of biopharmaceuticals.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Pharmacology & Pharmacy
Ravi Maharjan, Shavron Hada, Ji Eun Lee, Hyo-Kyung Han, Ki Hyun Kim, Hye Jin Seo, Camilla Foged, Seong Hoon Jeong
Summary: A combinatorial artificial-neural-network design-of-experiment (ANN-DOE) model was developed to evaluate the effects of various factors on the outcome responses of mRNA-LNP vaccine. The ANN-DOE model outperformed optimized ML models in predicting the bioprocess of the vaccine. Increased flow rate ratio (FRR) decreased particle size and increased zeta potential (ZP), while increased total flow rate (TFR) increased PDI and ZP. The ANN-DOE model demonstrated better predictive ability and accuracy compared to independent ML models.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
You Xu, Elisa Parra-Ortiz, Feng Wan, Olga Canadas, Begona Garcia-Alvarez, Aneesh Thakur, Henrik Franzyk, Jesus Perez-Gil, Martin Malmsten, Camilla Foged
Summary: Pulmonary delivery of siRNA using nanoparticle-based systems shows promise for treating respiratory diseases. We developed inhaler formulations of siRNA-loaded lipid-polymer hybrid nanoparticles and optimized them for inhalation therapy. The interaction between the nanoparticles and pulmonary surfactant was studied to understand the mechanisms involved. The results provide new insights into the design criteria for nanoparticle transport through the air-blood barrier.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Anas Aljabbari, Abhijeet Girish Lokras, Jacob Judas Kain Kirkensgaard, Thomas Rades, Henrik Franzyk, Aneesh Thakur, Yibang Zhang, Camilla Foged
Summary: We investigated the structural and material properties of siRNA-loaded LPNs using various analytical techniques. The results revealed the self-assembly of lipidoid into lamellar structures, the lateral organization of siRNA between lipidoid bilayers, and the core-shell structure of siRNA-loaded LPNs. The interactions between lipidoid and siRNA were observed, and the inclusion of siRNA affected the phase behavior of lipidoid. These findings contribute to the understanding of siRNA-loaded LPNs and their applications.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Instruments & Instrumentation
Janni Stovring Mortensen, Duc Ninh Nguyen, Hanne Morck Nielsen, Stine Ronholt
Summary: Early intervention and short-duration antibiotics treatment in premature infants can reduce the incidence of necrotizing enterocolitis (NEC), but it is unclear how antibiotics exposure and route of administration affect the disease risk. This study investigated the effects of ampicillin and gentamicin administered through parenteral or a combination of enteral and parenteral routes on the barrier properties of intestinal mucosa and mucus in preterm piglets. Results showed that permeation through mucosa and mucus was reduced in parenterally treated piglets compared to untreated piglets, while treatments with both routes showed similar permeation to untreated piglets. Rheological measurements also showed changes in mucus properties.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Marco Herrera-Barrera, Milan Gautam, Abhijeet Lokras, Kseniia Vlasova, Camilla Foged, Gaurav Sahay
Summary: Prime editing is an advanced gene editing platform that can correct almost any disease-causing mutation. This study developed lipid nanoparticles as delivery vehicles for prime editors, which resulted in improved efficiency and speed of editing. The use of these nanoparticles has the potential to revolutionize gene therapy and open up new possibilities for treatment.
Article
Chemistry, Multidisciplinary
Jessica Pihl, Thomas M. M. Clausen, Jiarong Zhou, Nishta Krishnan, Maj S. S. Orum-Madsen, Tobias Gustavsson, Robert Dagil, Mads Daugaard, Swati Choudhary, Camilla Foged, Jeffrey D. D. Esko, Liangfang Zhang, Ronnie H. H. Fang, Ali Salanti
Summary: Malaria-infected erythrocytes and oncofetal CS have distinct tropism and can potentially be used for cancer targeting. Researchers have developed a drug delivery platform using erythrocyte membrane-coated nanoparticles that mimic malaria-infected erythrocytes and specifically target and kill melanoma cells. This biomimetic approach shows potential as a broadly targeted cancer therapy.
Article
Pharmacology & Pharmacy
Aneesh Thakur, Abishek Wadhwa, Abhijeet Lokras, Olivia Amanda Oest Mullertz, Dennis Christensen, Henrik Franzyk, Camilla Foged
Summary: The incorporation of ionizable lipid L5N12 in liposomal cationic adjuvant CAF (R) 09 maintains the adjuvant function and induces antigen-specific immune responses comparable to unmodified CAF (R) 09.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Marie Hellfritzsch, Dennis Christensen, Camilla Foged, Regina Scherliess, Aneesh Thakur
Summary: Reconstituted dry powder formulations of ZnO nanoparticles can induce antigen-specific antibodies and can be used in vaccines to enhance antigen-specific humoral immune responses against subunit protein antigens.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
