Article
Virology
Abbas Soleimani-Delfan, Majid Bouzari, Ran Wang
Summary: The newly discovered E. faecalis bacteriophage vB_EfaS-DELF1 is specific, effective against vancomycin-resistant strains, lacks undesirable genes in its genome, and remains stable under various environmental conditions.
Article
Engineering, Environmental
Rui Gao, Miao Yu, Jingni Xie, Minghao Sui
Summary: This study comparatively investigated the efficacy of UV and UV/H2O2 in inactivating vancomycin-resistant Enterococcus faecalis (VRE) and degrading its containing vanB gene. The results showed that VRE was readily inactivated in both UV and UV/H2O2 processes. UV/H2O2 achieved synergistic degradation of intracellular vanB and inhibited bacterial regrowth. The presence of humic acid inhibited VRE inactivation and vanB degradation, but lower concentrations of humic acid slightly promoted vanB degradation in UV/H2O2 process.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Food Science & Technology
Marlena Golas-Pradzynska, Magdalena Luszczynska, Jolanta Grazyna Rola
Summary: This study investigates the antimicrobial resistance, virulence, and resistance genes of Enterococcus faecalis and Enterococcus faecium isolated from raw goat's and sheep's milk and cheese. The results show that E. faecalis is the dominant species, with high levels of antimicrobial resistance and presence of virulence genes in some strains.
Article
Microbiology
Siheng Wang, Chang Cai, Yingbo Shen, Chengtao Sun, Qingxin Shi, Ningjun Wu, Shufang Zheng, Jiao Qian, Rong Zhang, Hongwei Zhou
Summary: Contezolid exhibits potent activity against MRSA and VRE strains. It shows similar or slightly better antimicrobial activity compared to linezolid. Contezolid may have potential benefits for treating infections caused by MRSA and VRE pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Fuqiang Song, Jun Sheng, Jishan Tan, Huajie Xie, Xiaoyu Wang, Wenqiong Guo
Summary: In this study, a bacteriophage SFQ1 was isolated from hospital sewage, which effectively infects E. faecalis strain EFS01 and disrupts the biofilms formed by E. faecalis. Therefore, SFQ1 has the potential for treating E. faecalis infections.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Ophthalmology
Seung Min Lee, Jong Ho Park, Chul Hun Jang, Iksoo Byon
Summary: Intravitreal injection of povidone-iodine was effective in treating VRE endophthalmitis, even at low concentrations. High-concentration intravitreal vancomycin injection can also be used to treat resistant strains within the safety threshold.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Kushal Vanamala, Ketki Bhise, Hiram Sanchez, Razieh Kebriaei, Duy Luong, Samaresh Sau, Hosam Abdelhady, Michael J. Rybak, David Andes, Arun K. Iyer
Summary: This study aims to improve the efficacy of VAN treatment by identifying the folate receptor for MRSA infected tissues and developing folate decorated lipid nanoparticles containing VAN (LVAN). In comparison to conventional VAN, LVAN showed a higher bactericidal effect and a superior ability to inhibit biofilm in MRSA with an enhanced accumulation in MRSA infected thigh tissues and a reduced accumulation in kidney. The results suggested that LVAN is a promising candidate to overcome the current limitations of bacterial resistance and adverse side effects in kidneys found in VAN.
Review
Microbiology
Adrianna M. Turner, Jean Y. H. Lee, Claire L. Gorrie, Benjamin P. Howden, Glen P. Carter
Summary: This review provides an overview of resistance to last-line antimicrobial agents in Staphylococcus and VRE, particularly focusing on how genomics has provided insights into the emergence of resistant clones, the molecular mechanisms of resistance, and the importance of mobile genetic elements in the global spread of resistance to linezolid.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Melissa J. Karau, Suzannah M. Schmidt-Malan, Scott A. Cunningham, Jayawant N. Mandrekar, Bobbi S. Pritt, Tiffany R. Keepers, Alisa W. Serio, Surya Chitra, Robin Patel
Summary: Omadacycline, vancomycin, and rifampin, as well as rifampin combination therapies, were evaluated in an experimental rat model of MRSA osteomyelitis. The results showed that rifampin combination therapy was effective in reducing MRSA recovery without developing resistance. The combination of omadacycline and rifampin yielded negative MRSA detection, suggesting its potential as a treatment for osteomyelitis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Beatriz Rizzo Paschoalini, Karen Vanessa Munive Nunez, Juliana Takahashi Maffei, Helio Langoni, Felipe Freitas Guimaraes, Clarice Gebara, Natylane Eufransino Freitas, Marcos Veiga dos Santos, Carlos Eduardo Fidelis, Roberto Kappes, Monica Correia Goncalves, Nathalia Cristina Cirone Silva
Summary: This study evaluates the antimicrobial resistance and virulence characteristics of Enterococcus spp. isolated from bovine milk, showing high resistance in E. faecium and E. faecalis. The findings highlight the potential risks and the need for control strategies in dairy farms.
Article
Chemistry, Multidisciplinary
Le Wang, Wenfu Zheng, Leni Zhong, Yingkun Yang, Yao Chen, Qinghong Hou, Peiyuan Yu, Xingyu Jiang
Summary: This study focuses on the issues of drug resistance and high toxicity in vancomycin, and presents mercaptophenylboronic acid (MBA)-modified gold nanoclusters (Au-44(MBA)(18)) as a solution. The results show that Au-44(MBA)(18) exhibits excellent antibacterial activities against drug-resistant bacteria and can interact with bacteria by attaching to teichoic-acid and destroying the cell wall, as well as binding to bacterial DNA. It has multiple administration routes and high biosafety, making it a potential nanoantibiotic for treating multidrug-resistant bacterial infections.
Article
Infectious Diseases
Sonia Nina Coccitto, Marzia Cinthi, Simona Fioriti, Gianluca Morroni, Serena Simoni, Carla Vignaroli, Cristiana Garofalo, Marina Mingoia, Andrea Brenciani, Eleonora Giovanetti
Summary: This study characterized a linezolid-resistant Enterococcus gallinarum isolate from pigs that carried the cfr, optrA, and poxtA genes. Two plasmids were identified, each carrying different resistance genes. The plasmids showed features of circular intermediates and were not transferable to other enterococcal strains. The persistence of these plasmids in the absence of selective pressure is concerning.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Jiazhen Liu, Yanpeng Zhu, Yang Li, Yuwen Lu, Kun Xiong, Qiu Zhong, Jing Wang
Summary: In this study, a bacteriophage EFap02 with the potential for treating drug-resistant Enterococcus faecalis infection was isolated. The phage proved to be tolerant to harsh environments and high temperature. However, phage-resistant mutants were found to have impaired biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Martin Villegas, Claudia Alonso-Cantu, Sara Rahmani, David Wilson, Zeinab Hosseinidoust, Tohid F. Didar
Summary: Medical device-associated infections are a persistent issue, and a multifunctional coating combining LIS with tethered antibiotics has been developed to effectively reduce biofilm formation and maintain antibiotic activity, providing a reliable strategy for preventing bacteria-associated infections.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Microbiology
Kodjovi D. Mlaga, Vincent Garcia, Philippe Colson, Raymond Ruimy, Jean-Marc Rolain, Seydina M. Diene
Summary: The study conducted a comparative genomic analysis of E. faecalis and E. faecium genomes, revealing a significant difference in the presence of CRISPR-Cas systems between the two species. The research also showed a correlation between the number of CRISPR-Cas systems, genome size, %GC content, and the acquisition of mobile genetic elements, including antimicrobial resistance genes. Furthermore, the presence of the anti-endonuclease ardA gene in E. faecium may explain the decrease in CRISPR-Cas systems and the association with vancomycin resistance genes.
Review
Chemistry, Multidisciplinary
Carlos A. Garcia-Gonzalez, Alejandro Sosnik, Jozsef Kalmar, Iolanda De Marco, Can Erkey, Angel Concheiro, Carmen Alvarez-Lorenzo
Summary: Aerogels, as the lightest solid materials on Earth, have shown great potential in drug delivery due to their high porosity and specific surface area. By tuning the aerogel structure and functionalization, site-specific, stimuli-responsive, and prolonged drug release can be achieved. This review highlights the recent advances in aerogels for drug delivery, focusing on loading efficiency, release kinetics, therapeutic demands, as well as gene therapy and life cycle assessment.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Multidisciplinary Sciences
Cristina Bellotti, Kristina Lang, Nataliya Kuplennik, Alejandro Sosnik, Robert Steinfeld
Summary: A new two-step fast performance liquid chromatography (FPLC) protocol has been developed for the purification of large numbers of extracellular vesicles (EVs), resulting in higher purity and more selective enrichment of exosomes. The method combines size exclusion chromatography with immobilized metal affinity chromatography for efficient separation of different types of EVs. This new approach holds promise for the isolation of defined subpopulations of EVs with preserved biological function that can be easily scaled up for production of larger amounts of EVs.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Roni Sverdlov Arzi, Asaf Kay, Yulia Raychman, Alejandro Sosnik
Summary: Nanoprecipitation and microfluidics technology are effective methods for producing pure drug nanoparticles and optimizing product performance. The use of specific microfluidic devices can lead to the production of rounded, optimized nanoparticles.
Review
Biotechnology & Applied Microbiology
Areen Ashkar, Alejandro Sosnik, Maya Davidovich-Pinhas
Summary: Oral administration is a popular route for drug delivery, but it faces challenges due to the involvement of the gastro-intestinal system and drug bioavailability. Researchers have developed strategies to improve drug solubility and permeability in the intestine by using lipid-based systems. This review provides an overview of different structured edible lipid-based oral delivery systems and emphasizes their contribution to drug delivery performance.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Materials Science, Biomaterials
Prakram Singh Chauhan, Murali Kumarasamy, Angel M. Carcaboso, Alejandro Sosnik, Dganit Danino
Summary: This study encapsulated pitavastatin (PTV) in silica-coated polymeric micelles surface-modified with a cyclic peptide targeting the integrin receptors to potentially treat GBM. The silica shell provided colloidal stability and controlled release kinetics, showing efficacy in inducing apoptosis in glioma cells and reducing cell viability. Overall, the results suggest the potential of these hybrid nanocarriers for targeted therapy of GBM and other integrin receptor-overexpressing tumors.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Guillem Pascual-Pasto, Helena Castillo-Ecija, Nora Unceta, Rosario Aschero, Claudia Resa-Pares, Alberto Gomez-Caballero, Monica Vila-Ubach, Oscar Munoz-Aznar, Mariona Sunol, Victor Burgueno, Soledad Gomez-Gonzalez, Alejandro Sosnik, Manuel Ibarra, Paula Schaiquevich, Enrique de Alava, Oscar M. Tirado, Jaume Mora, Angel M. Carcaboso
Summary: Secreted protein acidic and rich in cysteine (SPARC) promotes the accumulation of nanoparticle albumin-bound (nab)paclitaxel in pediatric sarcomas through its high affinity to albumin.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
R. A. Jimenez, D. Millan, A. Sosnik, M. R. Fontanilla
Summary: Microgels can absorb and retain a large amount of solvents, especially water, and the release kinetics of active molecules from microgels is easier to control than from hydrogels. In this study, cytocompatible collagen I microgels were produced by emulsification-gelation, and the effect of collagen I concentration and homogenization method on the properties of microgels was evaluated.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Engineering, Biomedical
Daniela Dobrynin, Iryna Polishchuk, Lotan Portal, Ivan Zlotver, Alejandro Sosnik, Boaz Pokroy
Summary: The Delta and Omicron variants of SARS-CoV-2 exhibit higher adsorption capacities compared to the original strain, indicating a correlation between adsorption capacity and transmissibility. Additionally, the S1 subunit shows strongest adsorption ability at physiological pH 7.4.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Multidisciplinary
Jiri Trousil, Nils-Jorgen K. Dal, Federico Fenaroli, Inbar Schlachet, Pavia Kubickova, Olga Janouskova, Ewa Pavlova, Misa Skoric, Katerina Trejbalova, Oto Pavlis, Alejandro Sosnik
Summary: Encapsulating levofloxacin in amphiphilic polymeric nanoparticles enhances antibacterial efficacy, with potential for targeting intracellular infections.
Review
Pharmacology & Pharmacy
Rania Awad, Avi Avital, Alejandro Sosnik
Summary: Neurodegenerative diseases and neurodevelopmental disorders are challenging to treat due to the blood-brain barrier. The nose-to-brain pathway offers a promising solution, especially for intranasally administered nanoparticle drugs. Polymeric nanocarriers have shown great potential in improving drug bioavailability in the brain. This review provides an overview of the challenges faced and the contribution of intranasal nano-drug delivery systems in treating CNS conditions.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Chemistry, Physical
Hen Moshe Halamish, Ivan Zlotver, Alejandro Sosnik
Summary: In this study, self-assembled multi-micellar colloidal systems of an amphiphilic poly(vinyl alcohol)-g-poly(methyl methacrylate) copolymer surface-modified with boric acid were designed for the active targeting of solid tumors that overexpress sialic acid. The results showed that surface boration increased the permeability and uptake of the nanoparticles, and in vivo experiments confirmed a significant increase in intertumoral accumulation of the modified nanocarriers.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Ivan Zlotver, Alejandro Sosnik
Summary: Sonodynamic therapy (SDT) is an effective anti-cancer strategy that utilizes sono-responsive hybrid nanomaterials to generate reactive oxygen species (ROS) upon ultrasound irradiation. This study synthesized and characterized sonodynamic hybrid nanomaterials composed of titanium dioxide and an amphiphilic block copolymer, and investigated their efficiency in vitro and in vivo. The results showed that glucosylated hybrid nanoparticles exhibited significantly higher sonodynamic efficiency compared to unmodified ones. Furthermore, by controlling the nanoparticle size and surface features, the accumulation of nanoparticles in tumors was increased, resulting in prolonged animal survival and significantly reduced tumor volumes in a mouse model of rhabdomyosarcoma.
Article
Materials Science, Biomaterials
Alejandro Sosnik, Ivan Zlotver, Ella Peled
Summary: This study investigates the effect of drug-free self-assembled hydrolyzed galactomannan-poly(methyl methacrylate) nanoparticles on the activation of macrophages. Results show that the nanoparticles are cell compatible, upregulate metallothionein genes, and induce the release of anti-inflammatory cytokines by macrophages. These findings suggest the potential application of hGM-g-PMMA nanoparticles in inflammation therapy.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Nanoscience & Nanotechnology
Alexandra Bukchin, Macarena Sanchez-Navarro, Adam Carrera, Claudia Resa-Pares, Helena Castillo-Ecija, Leire Balaguer-Lluna, Meritxell Teixido, Nagore G. Olaciregui, Ernest Giralt, Angel M. Carcaboso, Alejandro Sosnik
Summary: The study developed modified drug-loaded nanoparticles that can enhance transport across the blood-brain barrier and display anticancer activity in DIPG cells. These nanoparticles are mainly internalized by DIPG cells through clathrin-mediated endocytosis and show strong anticancer efficacy in vivo by killing a substantial amount of DIPG cells.
ACS APPLIED NANO MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Nicole Lecot, Romina Glisoni, Natalia Oddone, Juan Benech, Marcelo Fernandez, Juan Pablo Gambini, Pablo Cabral, Alejandro Sosnik
Summary: This study investigates the potential of glycosylation to enhance the internalization of nanodrug delivery systems in breast cancer cells, showing promising results.
ADVANCED THERAPEUTICS
(2021)