Secondary Metabolites of Phomopsis sp XZ-26, an Endophytic Fungus from Camptotheca acuminate

Eleven new metabolites, including nine lovastatin analogues [oblongolides N-V (1-2 and 5-11), which were defined as naphthalene-type fungal polyketides], one linear furanopolyketide (13) and a monoterpene named dihydroxysabinane (14), together with four known compounds including oblongolides B (3) and C (4), one linear furanopolyketide (12) and the sesterterpene terpestacin (15), were isolated from the endophytic fungal strain Phomopsis sp. XZ-26 of Camptotheca acuminate. Their structures were elucidated by spectroscopic analyses including HR-ESI-MS, H-1 and C-13 NMR, 2D NMR (HMQC, HMBC, H-1-H-1 COSY and NOESY), and X-ray single-crystal analysis. The antimicrobial activities of 1-5, 8, 10 and 13-15 were evaluated, but none showed a substantial effect. Additionally, a hypothetical biosynthetic pathway for oblongolides was proposed. ((C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)