期刊
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
卷 2008, 期 15, 页码 2539-2543出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ejoc.200800103
关键词
enzymes; ketones; asymmetric catalysis; reduction; biotransformations
资金
- Austrian Science Fund (FWF) [W 901] Funding Source: researchfish
Biocatalysts suitable for the reduction of ketones bearing two sterically demanding substituents (bulky-bulky ketones) at elevated substrate concentration (10 g L-1) were identified. The biocatalysts can be employed in a substrate-coupled approach; thus, a simple alcohol such as ethanol or 2-propanol serves as a hydrogen donor. Both enantiomers are accessible by using either Rhodococcus ruber DSM 44541 and ethanol or Ralstonia sp. DSM 6428 and Sphingobium yanoikuyae DSM 6900 and ethanol or 2-propanol as the hydrogen source. For Rhodococcus ruber DSM 44541, it was found that bulky-bulky ketones were only transformed when ethanol was used as a hydrogen source, whereas no conversion was observed when 2-propanol was employed. From the substrate spectrum, as well as from the cosubstrate preference, it became clear that a different alcohol dehydrogenase than the previously described ADH-A is active in the presence of ethanol in Rhodococcus ruber. ((c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008).
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