Article
Radiology, Nuclear Medicine & Medical Imaging
Ida Friberger, Joachim N. N. Nilsson, Li Lu, Jonathan Siikanen, Oscar Ardenfors, Stefan Milton, Erik Samen, Jeroen A. C. M. Goos, Mattias Carlsten, Staffan Holmin, Thuy A. A. Tran
Summary: The study assessed the in vivo biodistribution of cells in rats using two radiotracers, [Zr-89]Zr-(oxinate)(4) and [Zr-89]Zr-DFO-NCS. The results showed that the behavior of the radiolabelled cells in vivo was influenced by the labelling method. [Zr-89]Zr-(oxinate)(4) labelled cells had higher liver uptake, while [Zr-89]Zr-DFO-NCS labelled cells showed prolonged accumulation in the lungs. The difference in lung and liver uptake continued until day 7. Furthermore, dosimetry calculations showed different effective doses for the two radiotracers. The study highlights the importance of radiotracer labelling in cell biodistribution studies using PET.
Article
Radiology, Nuclear Medicine & Medical Imaging
Ida Friberger, Emma Jussing, Jinming Han, Jeroen A. C. M. Goos, Jonathan Siikanen, Helen Kaipe, Melanie Lambert, Robert A. Harris, Erik Samen, Mattias Carlsten, Staffan Holmin, Thuy A. Tran
Summary: This study successfully optimized the synthesis of [Zr-89]Zr-oxine and [Zr-89]Zr-DFO-NCS, demonstrating a straightforward protocol for cell labelling with high efficiency. The radiotracers were equally effective, but [Zr-89]Zr-oxine showed superiority in long-term stability, cellular retention, minimal variation between cell types, and cell labelling efficiency.
MOLECULAR IMAGING AND BIOLOGY
(2021)
Review
Oncology
Laura M. Lechermann, Doreen Lau, Bala Attili, Luigi Aloj, Ferdia A. Gallagher
Summary: This review discusses the potential of using non-invasive imaging methods for tracking therapeutic cells, particularly in oncology. The focus is on the role of radiopharmaceuticals for non-invasive detection and tracking of therapeutic cells using positron emission tomography (PET). Evaluation of PET imaging methods in preclinical cancer models and overcoming translational challenges are also examined to improve the accuracy and efficacy of cell therapies.
Review
Chemistry, Multidisciplinary
Alessia Peserico, Chiara Di Berardino, Valentina Russo, Giulia Capacchietti, Oriana Di Giacinto, Angelo Canciello, Chiara Camerano Spelta Rapini, Barbara Barboni
Summary: This review provides an overview of the applications of nanoparticles (NPs) in the diagnostic and therapeutic fields, specifically focusing on their interaction with cells and the imaging devices used for NP delivery assessment. The design and applications of cell membrane-coated NPs are considered mature, but there is still room for improvement in the design of NPs for directing cell labeling and intracellular retention.
Article
Medicine, Research & Experimental
Maxwell Ducharme, Lucinda Hall, Whitney Eckenroad, Shelbie J. Cingoranelli, Hailey A. Houson, Luke Jaskowski, Chanelle Hunter, Benjamin M. Larimer, Suzanne E. Lapi
Summary: HER2 overexpression is associated with aggressive forms of breast cancer. Nanobodies, such as 2Rs15d, have shown potential as imaging agents and therapeutics for targeting HER2. In this study, the imaging and binding properties of 2Rs15d were evaluated, demonstrating high-quality visualization of HER2-positive tumors.
MOLECULAR PHARMACEUTICS
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Sergio Munoz Vazquez, Heike Endepols, Thomas Fischer, Samir-Ghali Tawadros, Melanie Hohberg, Beate Zimmermanns, Felix Dietlein, Bernd Neumaier, Alexander Drzezga, Markus Dietlein, Klaus Schomaecker
Summary: A Zr-89-labeled inhibitor of prostate-specific membrane antigen (PSMA) was presented as a complement to established F-18 or Ga-68 ligands. The study showed promising results in terms of tumor imaging and detection of areas with lower PSMA expression, warranting further clinical evaluation.
MOLECULAR IMAGING AND BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Veronika Kozlovskaya, Maxwell Ducharme, Maksim Dolmat, James M. Omweri, Volkan Tekin, Suzanne E. Lapi, Eugenia Kharlampieva
Summary: Researchers developed a 60 nm-sized polymer vesicle that can be easily imaged using positron emission tomography (PET) without the need for chelators. By modifying the vesicle surface with the degradable tannic acid (TA) and targeting antibody trastuzumab (Tmab), the vesicles were able to specifically target HER2-positive breast cancer cells. These radiolabeled vesicles hold great potential for precise drug delivery and therapy.
Article
Engineering, Biomedical
Stefan Harmsen, Emin Ilker Medine, Maxim Moroz, Fuad Nurili, Jose Lobo, Yiyu Dong, Mezruh Turkekul, Naga Vara Kishore Pillarsetty, Richard Ting, Vladimir Ponomarev, Oguz Akin, Omer Aras
Summary: The adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells is a promising cancer therapy, and a dual-modal PET/NIRF nanoparticle-based imaging agent has been developed to monitor their biodistribution and fate in vivo, providing a new approach for imaging technology in other cancer models.
Article
Nanoscience & Nanotechnology
Pyeong Seok Choi, Jun Young Lee, Jung Ho Chae, Thaddeus Wadas, Zhen Cheng, Min Goo Hur, Jeong Hoon Park
Summary: Cherenkov radiation is being studied for cancer detection and therapy. Nanoparticles are used to develop a theranostic platform for visualizing therapy delivery and generating reactive oxygen species to kill cancer cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Oncology
Massis Krekorian, Kimberley R. G. Cortenbach, Milou Boswinkel, Annemarie Kip, Gerben M. Franssen, Andor Veltien, Tom W. J. Scheenen, Rene Raave, Nicolaas Koen van Riessen, Mangala Srinivas, Ingrid Jolanda M. de Vries, Carl G. Figdor, Erik H. J. G. Aarntzen, Sandra Heskamp
Summary: This study successfully demonstrated in vivo tracking of a small number of immune cells using zirconium-89-labeled poly(lactic-co-glycolic acid) nanoparticles, showing potential applications in various disease models.
Article
Multidisciplinary Sciences
Kazuo Narushima, Kiyohiko Shuto, Shinichi Okazumi, Gaku Ohira, Mikito Mori, Koichi Hayano, Noriyuki Yanagawa, Hisahiro Matsubara
Summary: This study retrospectively evaluated the preoperative PET scan results of 89 GIST patients and found that SUVmax was significantly correlated with tumor size, mitotic count, and Ki-67 index. When the SUVmax cutoff value was set at 5.68, the diagnostic accuracy for the high-risk group, recurrence group, and death group of GIST patients reached 86.5%, 76.4%, and 73.0% respectively. The group with SUVmax ≥ 5.68 had significantly lower 10-year relapse-free survival and overall survival compared to the group with SUVmax<5.68.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Kaivin Hadidi, Kfir B. Steinbuch, Lara E. Dozier, Gentry N. Patrick, Yitzhak Tor
Summary: Puromycin derivatives modified with azetidine and 3,3-difluoroazetidine exhibit translation inhibition and bactericidal activity similar to natural antibiotic. These analogues can puromycylate nascent peptides, generating emissive products without follow-up chemistry. The 3,3-difluoroazetidine-containing analogue can fluorescently label newly translated peptides and be visualized in live and fixed cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Oncology
Sarah R. Verhoeff, Sjoukje F. Oosting, Sjoerd G. Elias, Suzanne C. van Es, Sophie L. Gerritse, Lindsay Angus, Sandra Heskamp, Ingrid M. E. Desar, C. Willemien Menke-van der Houven van Oordt, Astrid A. M. van der Veldt, Anne I. J. Arens, Adrienne H. Brouwers, Bertha Eisses, Peter F. A. Mulders, Otto S. Hoekstra, Gerben J. C. Zwezerijnen, Winette T. A. van der Graaf, Erik H. J. G. Aarntzen, Wim J. G. Oyen, Carla M. L. van Herpen
Summary: Patients with metastatic clear-cell renal cell carcinoma (mccRCC) with good or intermediate prognosis can consider watchful waiting (WW), especially those who meet the watch and wait (W&W) criteria of < 2 International Metastatic RCC Database Consortium criteria and <=2 metastatic sites. This study aimed to assess the predictive value of [F-18]FDG PET/CT and [Zr-89]Zr-DFO-girentuximab PET/CT for the duration of WW in patients with mccRCC.
CLINICAL CANCER RESEARCH
(2023)
Article
Materials Science, Biomaterials
Pyeong Seok Choi, Jun Young Lee, Seung Dae Yang, Jeong Hoon Park
Summary: A novel chelator-free Zr-89-IONC with rice-shaped morphology was developed and coated with polyethyleneimine (PEI) and polyvinylpyrrolidone (PVP) to improve cancer targeting efficacy. Positron emission tomography measurements confirmed that only PVP-coated Zr-89-IONC reached the tumor region, while non-coated and PEI coated ones tended to be cleared in the liver and spleen. The Zr-89-incorporated iron oxide nanocomposite showed stability for radiolabeling, making it a potential carrier for specific cancer cell targeting.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Physical
Ji Wang, Shuqing Zhang, Yiming Han, Lilei Zhang, Qingyin Wang, Gongying Wang, Xiaoming Zhang
Summary: In this study, UiO-66 (Zr/Ti) materials with different Ti4+ contents were successfully synthesized and characterized. The results showed that Ti4+ was successfully integrated into the UiO-66 framework. Tests on the acidic properties of the samples revealed the presence of stable acidic sites. The experimental results also demonstrated the potential of UiO-66 (Zr/Ti) in the production of PET.
MOLECULAR CATALYSIS
(2022)
Review
Cell & Tissue Engineering
Brooke M. Helfer, Vladimir Ponomarev, P. Stephen Patrick, Philip J. Blower, Alexandra Feitel, Gilbert O. Fruhwirth, Shawna Jackman, Lucilia Pereira Mouries, Margriet V. D. Z. Park, Mangala Srinivas, Daniel J. Stuckey, Mya S. Thu, Tineke van den Hoorn, Carla A. Herberts, William D. Shingleton
Summary: Cell-based therapies are progressing towards clinical reality, yet face challenges in late-phase clinical trials. A survey conducted at an international conference revealed gaps and needs in safety evaluations for cell therapy developers. Understanding and utilizing imaging technologies are crucial for ensuring the safe and effective use of cellular therapeutics in clinical settings.
Article
Chemistry, Multidisciplinary
Angus J. Koller, Shefali Saini, Ivis F. Chaple, M. Andrey Joaqui-Joaqui, Brett M. Paterson, Michelle T. Ma, Philip J. Blower, Valerie C. Pierre, Jerome R. Robinson, Suzanne E. Lapi, Eszter Boros
Summary: This study fills the knowledge gap in stabilizing molecular Ti4+ species in aqueous environments through a thorough thermodynamic screening of bidentate chelators with Ti4+ in aqueous solution, as well as computational and structural analyses of key compounds. Furthermore, the chelation capacity of hexadentate analogues towards the Ti-45 isotope for positron emission tomography (PET) imaging applications was evaluated, showing excellent performance for kit-formulation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Wolfgang Maret, Philip Blower
Summary: Biochemistry often overlooks the importance of metal elements in life, but at least 10 essential chemical elements are metals. Introducing elemental biology into education, especially through the field of metallomics, can fill a major gap and provide students with a broader understanding of life processes.
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
(2022)
Review
Chemistry, Multidisciplinary
Peter J. Gawne, Francis Man, Philip J. Blower, Rafael T. M. de Rosales
Summary: The arrival of cell-based therapies has revolutionized medicine. Radionuclide imaging, such as SPECT or PET, is a highly sensitive and quantitative imaging technique that has advantages for cell tracking. Direct cell radiolabeling, using a variety of chemical approaches, is a simple and reliable method for labeling cells without genetic modification.
Article
Biochemical Research Methods
Alex Rigby, George Firth, Charlotte Rivas, Truc Pham, Jana Kim, Andreas Phanopoulos, Luke Wharton, Aidan Ingham, Lily Li, Michelle Ma, Chris Orvig, Philip J. Blower, Samantha Y. A. Terry, Vincenzo Abbate
Summary: Auger electron therapy utilizes low-energy electrons emitted during radioactive decay to achieve cytotoxicity. However, suitable bifunctional chelators to incorporate Tl3+ into bioconjugates have not been developed. In this study, several multidentate N- and O-donor chelators were synthesized and shown to form stable complexes with (201)T1, exhibiting improved serum stability. By conjugating H(4)pypa to prostate-specific membrane antigen, tumor imaging was achieved in vivo, although tumor targeting efficacy needs improvement.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Kazumi Chia, Rowena L. Paul, Amanda J. Weeks, Marium Naeem, Gregory E. D. Mullen, David Landau, Philip J. Blower
Summary: This study compares different PET hypoxia tracers with radioresistance and HIF1α expression levels and identifies a mismatch in pO2 thresholds between the tracers and other markers of hypoxia. The uptake of the tracers only occurs at oxygen levels significantly lower than those inducing radioresistance, pimonidazole uptake, and HIF1α expression. These PET tracers may play a key role in predicting outcomes in stereotactic radiation therapy.
NUCLEAR MEDICINE AND BIOLOGY
(2022)
Article
Biophysics
Agostino Cilibrizzi, Julie Tzu-Wen Wang, Siham Memdouh, Antonella Iacovone, Kate McElroy, Noor Jaffar, Jennifer Denise Young, Robert C. Hider, Philip Blower, Khuloud Al-Jamal, Vincenzo Abbate
Summary: A series of NIR fluorescent probes targeting prostate-specific membrane antigen (PSMA) were developed, and PSMA-Cy7.5_2 demonstrated high affinity for PSMA in vitro, but its tumor accumulation in vivo was not significant.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Johanna Pruller, Truc Thuy Pham, Julia E. Blower, Putthiporn Charoenphun, Alessia Volpe, Kavitha Sunassee, Gregory E. D. Mullen, Philip J. Blower, Richard A. G. Smith, Michelle T. Ma
Summary: This study presents a new method for cell tracking, which involves labeling peptides that bind to the cell membrane, enabling quantitative and longitudinal tracking of cells in vivo. The method has been successfully demonstrated to track the accumulation of myeloma cells in the lungs.
RSC CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Ingebjorg N. Hungnes, Truc Thuy Pham, Charlotte Rivas, James A. Jarvis, Rachel E. Nuttall, Saul M. Cooper, Jennifer D. Young, Philip J. Blower, Paul G. Pringle, Michelle T. Ma
Summary: We have developed a diphosphine (DP) platform for radiolabeling peptides with 99mTc and 64Cu for molecular SPECT and PET imaging, respectively. The platform enables straightforward functionalization of targeting peptides with a diphosphine chelator, leading to bioconjugates that can be radiolabeled with high radiochemical yield. The DP platform offers versatility and potential for the development of new receptor-targeted imaging radiotracers.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Charlotte Rivas, Jessica A. Jackson, Alex Rigby, James A. Jarvis, Andrew J. P. White, Philip J. Blower, Andreas Phanopoulos, Michelle T. . Ma
Summary: Hydroxypyridinone-based chelators have been found to be useful in incorporating radioactive metal ions into nuclear medicine diagnostic and therapeutic agents. In this study, two novel chelators with hydroxypyridinone groups were prepared and their reactivity towards radiometal-mediated hydrolysis was investigated. The results showed that the L1 complex exhibited a higher reactivity towards hydrolysis compared to the L2 complex. These findings were further supported by density functional theory calculations. This study highlights the importance of proper characterization of radiolabeled products.
INORGANIC CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jitesh Chauhan, Melanie Grandits, Lais C. G. F. Palhares, Silvia Mele, Mano Nakamura, Jacobo Lopez-Abente, Silvia Crescioli, Roman Laddach, Pablo Romero-Clavijo, Anthony Cheung, Chara Stavraka, Alicia M. M. Chenoweth, Heng Sheng Sow, Giulia Chiaruttini, Amy E. E. Gilbert, Tihomir Dodev, Alexander Koers, Giulia Pellizzari, Kristina M. M. Ilieva, Francis Man, Niwa Ali, Carl Hobbs, Sara Lombardi, Daniel A. Lionarons, Hannah J. J. Gould, Andrew J. J. Beavil, Jenny L. C. Geh, Alastair D. MacKenzie D. Ross, Ciaran Healy, Eduardo Calonje, Julian Downward, Frank O. O. Nestle, Sophia Tsoka, Debra H. H. Josephs, Philip J. J. Blower, Panagiotis Karagiannis, Katie E. E. Lacy, James Spicer, Sophia N. N. Karagiannis, Heather J. J. Bax
Summary: Despite checkpoint inhibitor therapies, outcomes for 50% of melanoma patients remain poor. Melanoma-associated antigen CSPG4 is expressed in approximately 70% of cases, making it a potential target for effective immunotherapies. Using monoclonal IgE antibodies engineered with human constant domains to recognize CSPG4, we found that IgE binds to human melanomas, induces antibody-dependent cellular cytotoxicity, and stimulates pro-inflammatory responses in Fc-receptor-expressing monocytes. IgE demonstrates anti-tumor activity in melanoma xenograft models, enhancing macrophage infiltration and pro-inflammatory signaling pathways in the tumor microenvironment. Our findings suggest that IgE-based immunotherapy holds promise for melanoma treatment.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Afnan M. F. Darwesh, Cinzia Imberti, Joanna J. Bartnicka, Fahad Al-Salemee, Julia E. Blower, Alex Rigby, Jayanta Bordoloi, Alex Griffiths, Michelle T. Ma, Philip J. Blower
Summary: This study used radiotracers to examine the absorption, delivery, and mode of action of KP46, an experimental anticancer drug. The results showed that KP46 acts as a slow releaser of gallium ions and is inefficiently absorbed and delivered to tissues, including tumors.
Article
Multidisciplinary Sciences
George P. Keeling, Friedrich Baark, Orestis L. Katsamenis, Jing Xue, Philip J. Blower, Sergio Bertazzo, Rafael T. M. de Rosales
Summary: In this study, we compared the PET imaging of the BP tracer [Ga-68]Ga-THP-Pam and [F-18]NaF in a rat model of EC. The results showed that [Ga-68]Ga-THP-Pam was able to detect EC more sensitively.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
George Firth, Zilin Yu, Joanna J. Bartnicka, David Parker, Jana Kim, Kavitha Sunassee, Hannah E. Greenwood, Fahad Al-Salamee, Maite Jauregui-Osoro, Alberto Di Pietro, Joanna Guzman, Philip J. Blower
Summary: Non-invasive imaging techniques to dynamically map whole-body trafficking of essential metals in vivo in health and diseases are needed. Despite Zn-62 having appropriate physical properties for positron emission tomography (PET) imaging (half-life, 9.3 h; positron emission, 8.2%), its complex decay via Cu-62 (half-life, 10 min; positron emission, 97%) has limited its use. We aimed to develop a method to extract Zn-62 from a Zn-62/Cu-62 generator, and to investigate its use for in vivo imaging of zinc trafficking despite its complex decay.
Article
Chemistry, Inorganic & Nuclear
Giuseppe Floresta, Siham Memdouh, Truc Pham, Michelle T. Ma, Philip J. Blower, Robert C. Hider, Vincenzo Abbate, Agostino Cilibrizzi
Summary: Expression of the cellular transmembrane receptor alpha v beta 6 integrin is mainly found in malignant epithelial cells and could be a promising target for tumor imaging and therapy. This study successfully synthesized and labeled two Ga-68 PET probes for in vivo monitoring of alpha v beta 6 integrin. The probes showed high radiochemical yield and binding affinity in cellular studies.
DALTON TRANSACTIONS
(2022)