Article
Chemistry, Multidisciplinary
Mimi Shin, B. Jill Venton
Summary: In this study, we developed fast-scan cyclic voltammetry (FSCV) to measure dopamine release in fruit flies during behavior. The results showed that sugar feeding induced longer dopamine release compared to acetylcholine stimulation in the mushroom body. In addition, administering dopamine transporter inhibitor or D2 receptor antagonist significantly increased sugar-evoked dopamine release.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Cell Biology
Vladimir P. Grinevich, Amir N. Zakirov, Uliana V. Berseneva, Elena V. Gerasimova, Raul R. Gainetdinov, Evgeny A. Budygin
Summary: Fast-scan cyclic voltammetry (FSCV) is a technique used for real-time monitoring of neurotransmitter dynamics, which has significant implications in the study of neuropsychiatric disorders.
Article
Chemistry, Analytical
Michelle Hadad, Nadine Hadad, Alexander G. Zestos
Summary: In this study, a method for measuring cortisol using carbon fiber microelectrodes and fast-scan cyclic voltammetry was developed. This method showed high sensitivity, high spatiotemporal resolution, and biocompatibility, and it was able to detect cortisol stably. Its application will contribute to a better understanding of the physiological importance of cortisol.
Article
Chemistry, Multidisciplinary
Yumin Kang, Abhinav Goyal, Sangmun Hwang, Cheonho Park, Hyun U. Cho, Hojin Shin, Jinsick Park, Kevin E. Bennet, Kendall H. Lee, Yoonbae Oh, Dong Pyo Jang
Summary: FSCV is a technique for measuring neurotransmitter release with millisecond temporal resolution, capable of measuring concentrations down to the nanomolar scale. By optimizing the technique, the detection limit of FSCV can be further lowered.
Article
Chemistry, Multidisciplinary
Jaekyung Kim, Abhijeet S. Barath, Aaron E. Rusheen, Juan M. Rojas Cabrera, J. Blair Price, Hojin Shin, Abhinav Goyal, Jason W. Yuen, Danielle E. Jondal, Charles D. Blaha, Kendall H. Lee, Dong Pyo Jang, Yoonbae Oh
Summary: This study developed a computational technique using a statistical model for standardized, unbiased analysis of experimental MCSWV data for unbiased quantification of tonic DA. The technique showed reliable and improved estimation of tonic DA levels in vivo compared to conventional manual post-processing methods.
Article
Electrochemistry
Chase Stucky, Michael A. Johnson
Summary: In this study, it was found that three different SSRIs significantly decrease the oxidation current of serotonin when using the commonly used Jackson waveform. Coating carbon-fiber microelectrodes with Nafion further reduces the current and increases response times. To address fouling issues, an extended serotonin waveform was used, which alleviated the decrease in current, indicating reduced electrode fouling.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Biochemical Research Methods
Noel Manring, Muzammil M. N. Ahmed, Jessica L. Smeltz, Pavithra Pathirathna
Summary: The etiology of neurodegenerative diseases is poorly understood and the heavy metal copper has been found to play a critical role in neurotoxicity, affecting the development of these diseases. Analyzing heavy metals in the brain is challenging due to the limitations of existing metal detection tools, resulting in inadequate data for developing efficient therapeutics. This study developed a surface-modified electrode using fast-scan cyclic voltammetry to detect ultra-low concentrations of copper in the brain accurately and in real time.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sergio Hidalgo, Nicolas Fuenzalida-Uribe, Daniela Molina-Mateo, Angelica P. Escobar, Carlos Oliva, Rodrigo A. Espana, Maria Estela Andres, Jorge M. Campusano
Summary: This study introduces a method using fast scan cyclic voltammetry to record the release of endogenous amines in the adult fly brain. The research sheds light on how aminergic neural circuits influence behaviors in fruit flies, with findings showing an increase in octopamine and serotonin levels in response to electric shock and olfactory cues respectively in the calyx region associated with olfactory memory generation in flies.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Immunology
Melinda Hersey, Jennifer L. Woodruff, Nicholas Maxwell, Alia T. Sadek, Maria K. Bykalo, Ian Bain, Claudia A. Grillo, Gerardo G. Piroli, Parastoo Hashemi, Lawrence P. Reagan
Summary: This study examined inflammatory markers and serotonergic dynamics in co-morbid obesity and depression, finding that a high-fat diet led to obesity and depressive-like symptoms in rats, marked by increased pro-inflammatory cytokines in the hippocampus. Additionally, impaired response to escitalopram in the hippocampus of obese rats was linked to elevated neuroinflammation and increased serotonin transporter expression.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Biochemistry & Molecular Biology
Alyssa M. West, Katherine M. Holleran, Sara R. Jones
Summary: The serotonin and kappa opioid receptor (KOR) systems play important roles in disorders of negative affect. In this study, the researchers investigated the effects of KOR activation and serotonin reuptake inhibition on serotonin transmission in the substantia nigra pars reticulata (SNr). They found that KOR activation reduced serotonin release and uptake, while serotonin reuptake inhibition increased serotonin release and slowed reuptake. The researchers also demonstrated that short-term activation of KOR altered serotonin responses to reuptake inhibition. These findings highlight the complex interactions between KORs and serotonin in regulating negative affect, and suggest that the KOR system may be a promising target for treating disorders such as anxiety and depression alongside traditional antidepressant treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Edoardo Cuniberto, Zhujun Huang, Michael D. Ward, Davood Shahrjerdi
Summary: Fast-scan cyclic voltammetry (FSCV) with micron-sized carbon sensors is a promising method for monitoring serotonin (5-HT) neuromodulatory signals in the brain. However, the complex chemical reactions associated with 5-HT can compromise sensor performance. This study used engineered graphitic sensors to characterize the electrochemistry of 5-HT and optimize the FSCV conditions for quantitative in vivo detection. The results revealed the significant role of water in driving side reactions and provided new insights for more reliable 5-HT detection.
Review
Neurosciences
Suelen Lucio Boschen, James Trevathan, Seth A. Hara, Anders Asp, J. Luis Lujan
Summary: FSCV has been used for detecting electroactive neurotransmitters in animal models for decades, and is now being explored for human neurochemical studies. However, technical challenges hinder its clinical implementation, requiring more pre-clinical studies and risk management to ensure patient safety.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Chemistry, Analytical
Romana Jarosova, Adam D. Douglass, Michael A. Johnson
Summary: In this study, a waveform for the measurement of oxytocin was optimized using carbon-fiber microelectrodes and flow injection analysis. The method achieved a low limit of quantitation and was successfully applied to measure oxytocin release in the brains of genetically engineered zebrafish.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yuxin Li, Romana Jarosova, Moriah E. Weese-Myers, Ashley E. Ross
Summary: Graphene-fiber microelectrodes (GFME's) have been synthesized and characterized for subsecond detection of neurochemicals with fast-scan cyclic voltammetry (FSCV) for the first time. These microelectrodes exhibit faster electron transfer kinetics, significantly improved sensitivity, and ease of tunability, making them highly impactful for neurochemical detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Summary: It is widely believed that ascorbic acid (AA) interferes with the detection of neurotransmitters using electrochemical methods. However, this study shows that AA concentration decreases rapidly in cell culture medium, eventually becoming undetectable by electrodes, allowing for effective monitoring of neurotransmitter concentrations.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
David M. Hedges, J. Daniel Obray, Jordan T. Yorgason, Eun Young Jang, Vajira K. Weerasekara, Joachim D. Uys, Frederick P. Bellinger, Scott C. Steffensen
NEUROPSYCHOPHARMACOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Karl T. Schmidt, Viren H. Makhijani, Kristen M. Boyt, Elizabeth S. Cogan, Dipanwita Pati, Melanie M. Pina, Isabel M. Bravo, Jason L. Locke, Sara R. Jones, Joyce Besheer, Zoe A. McElligott
ACS CHEMICAL NEUROSCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Anushree N. Karkhanis, Amy C. Leach, Jordan T. Yorgason, Ayse Uneri, Samuel Barth, Farr Niere, Nancy J. Alexander, Jeffrey L. Weiner, Brian A. McCool, Kimberly F. Raab-Graham, Mark J. Ferris, Sara R. Jones
ACS CHEMICAL NEUROSCIENCE
(2019)
Article
Neurosciences
Cody A. Siciliano, Madelyn Mauterer, Steve C. Fordahl, Sara R. Jones
EUROPEAN JOURNAL OF NEUROSCIENCE
(2019)
Article
Neurosciences
Katherine M. Holleran, Jamie H. Rose, Steven C. Fordahl, Kelsey C. Benton, Kayla E. Rohr, Paul J. Gasser, Sara R. Jones
EUROPEAN JOURNAL OF NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
David M. Hedges, Jordan T. Yorgason, Andrew W. Perez, Nathan D. Schilaty, Benjamin M. Williams, Richard K. Watt, Scott C. Steffensen
Article
Biochemistry & Molecular Biology
James N. Brundage, Colin P. Mason, Hillary A. Wadsworth, Chris S. Finuf, Josh J. Nelson, P. Joakim W. Ronstrom, Sara R. Jones, Cody A. Siciliano, Scott C. Steffensen, Jordan T. Yorgason
Summary: Striatal dopamine release dynamics differ across sexes, with female mice showing less regional differences in dopamine release compared to male mice. Blocking voltage-gated K+ channels with 4-aminopyridine had the greatest effects in ventral regions of female mice, indicating regional differences in Kv channel expression. The dopamine transporter blocker cocaine enhanced detection across subregions in both sexes, with females showing greater overall increased release than males.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Neurosciences
Brianna E. George, Samuel H. Barth, Lindsey B. Kuiper, Katherine M. Holleran, Ryan T. Lacy, Kimberly F. Raab-Graham, Sara R. Jones
Summary: Evidence suggests that females are more vulnerable to the harmful effects of drugs like opioids, with increased heroin-related deaths seen in females. While males and females showed similar acquisition rates for heroin self-administration (SA), females exhibited increased responding and intake, seeking behavior, and escalation. The study found no differences in mu-opioid receptor (MOR) expression levels between males and females in the brain, but observed differential dopamine terminal adaptations in females that may contribute to their increased heroin-seeking behavior.
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Substance Abuse
Solal Bloch, Katherine M. Holleran, Thomas L. Kash, Elena M. Vazey, Jennifer A. Rinker, Christina L. Lebonville, Krysten O'Hara, Marcelo F. Lopez, Sara R. Jones, Kathleen A. Grant, Howard C. Becker, Patrick J. Mulholland
Summary: Alcohol use disorder frequently co-occurs with mood disorders, contributing to alcohol dependence and relapse. However, studies using different exposure models show variable outcomes in measuring negative affective behaviors.
Article
Biochemistry & Molecular Biology
Alyssa M. West, Katherine M. Holleran, Sara R. Jones
Summary: The serotonin and kappa opioid receptor (KOR) systems play important roles in disorders of negative affect. In this study, the researchers investigated the effects of KOR activation and serotonin reuptake inhibition on serotonin transmission in the substantia nigra pars reticulata (SNr). They found that KOR activation reduced serotonin release and uptake, while serotonin reuptake inhibition increased serotonin release and slowed reuptake. The researchers also demonstrated that short-term activation of KOR altered serotonin responses to reuptake inhibition. These findings highlight the complex interactions between KORs and serotonin in regulating negative affect, and suggest that the KOR system may be a promising target for treating disorders such as anxiety and depression alongside traditional antidepressant treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Anushree N. Karkhanis, Alyssa M. West, Sara R. Jones
Summary: The nucleus accumbens (NAc) core plays a role in regulating stress and shaping reward seeking behaviours. The activation of kappa opioid receptor (KOR) inhibits dopamine release and transmission, and this impact is regionally specific in the NAc shell. The inhibition of dopamine release by KOR is greater in the caudal compared to the rostral subregion of NAc core, suggesting a differential regulation along the rostro-caudal axis.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Meeting Abstract
Substance Abuse
A. N. Karkhanis, D. Leussen, J. L. Weiner, R. Chen, M. Bruchas, S. R. Jones
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2019)
Meeting Abstract
Substance Abuse
K. M. Holleran, S. R. Jones
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2019)
Article
Biology
Madelyn Mauterer, Paige M. Estave, Katherine M. Holleran, Sara R. Jones
Meeting Abstract
Substance Abuse
A. N. Karkhanis, D. Leussen, J. L. Weiner, R. Chen, M. Bruchas, S. R. Jones
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2018)