Article
Biochemistry & Molecular Biology
Rut Gabarro-Solanas, Amarbayasgalan Davaatseren, Justus Kleifeld, Tatjana Kepcija, Thomas Koecher, Albert Giralt, Ivan Crespo-Enriquez, Noelia Urban
Summary: Combining genetic lineage tracing and label retention experiments, this study shows that every-other-day intermittent fasting does not affect adult neural stem cell behavior and, despite previous encouraging data, is not a reliable strategy to promote adult hippocampal neurogenesis in mice.
Article
Geriatrics & Gerontology
Melissa C. Orenduff, Michael F. Coleman, Elaine M. Glenny, Kim M. Huffman, Erika T. Rezeli, Akshay Bareja, Carl F. Pieper, Virginia B. Kraus, Stephen D. Hursting
Summary: Aging is a complex process that involves the progressive deterioration of physiological integrity and function. Loss of muscle mass, strength, and function, known as sarcopenia, is one of the major contributors to the harmful effects of aging. Calorie restriction (CR) has been shown to extend lifespan and delay the onset and progression of age-related diseases, including sarcopenia. Rapamycin, a pharmacological compound, has demonstrated similar longevity benefits without the need for dietary restrictions. In our study, we found that both CR and rapamycin can partially protect against age-related muscle decline, but they engage different molecular pathways in skeletal muscle. The therapeutic potential of rapamycin or a CR regimen in preventing and managing sarcopenia should be further explored in translational studies.
EXPERIMENTAL GERONTOLOGY
(2022)
Article
Cell Biology
Sai Krupa Das, Rachel E. Silver, Alistair Senior, Cheryl H. Gilhooly, Manjushri Bhapkar, David Le Couteur
Summary: This study used the Geometric Framework for Nutrition to examine the impact of macronutrient composition on adherence to calorie restriction (CR) and its effect on cardiometabolic markers of healthspan. The study found that a higher percentage of reported energy intake from protein and carbohydrate, and lower fat intake, were associated with greater adherence to CR. However, there was no significant correlation between macronutrient composition and cardiometabolic risk factors in the CR group. This study suggests that individual macronutrients have an interactive role in CR adherence, but further research is needed to understand the effect of different macronutrient compositions on adherence to CR and cardiometabolic risk factors.
Article
Cell Biology
Francesco Annunziata, Seyed Mohammad Mahdi Rasa, Anna Krepelova, Jing Lu, Alberto Minetti, Omid Omrani, Suneetha Nunna, Lisa Adam, Sandra Kaeppel, Francesco Neri
Summary: Calorie restriction increases intestinal stem cell competition, but old mice have more competition than young mice. Late-life calorie restriction decreases this process. There is a strong correlation between Paneth cell number and intestinal stem cell competition, and increasing Paneth cell number can delay the fixation of mutated intestinal stem cells.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Libia Alejandra Garcia-Flores, Cara L. Green, Sharon E. Mitchell, Daniel E. L. Promislow, David Lusseau, Alex Douglas, John R. Speakman
Summary: Caloric restriction affects the metabolome in a tissue-specific manner, with only a small number of common metabolic features responding in the same way across all tissues. The top modulated pathways were related to biosynthesis and degradation processes, suggesting each tissue has its own unique way of coping with reduced energy intake, supporting the clean cupboards hypothesis over the disposable soma interpretation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Geriatrics & Gerontology
Satchidananda Panda, Geraldine Maier, Dennis T. Villareal
Summary: With the rise in obesity across age groups, engaging in physical activity and mobility in older adults has become difficult. Calorie restriction (CR) up to 25% has been used for obesity management, but its safety in older adults is not fully understood. There are challenges in adopting and complying with CR, and concerns about the negative effects on sarcopenia, osteopenia, and frailty. Time-Restricted Feeding/Eating (TRF/TRE), which optimizes circadian rhythm and combines with CR, may reduce weight and improve health.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Review
Nutrition & Dietetics
Emiliana Giacomello, Luana Toniolo
Summary: Aging is a biological process influenced by multiple cellular mechanisms leading to functional decline. The growth of the elderly population and their susceptibility to diseases drive the search for anti-aging interventions, with calorie restriction identified as an effective approach.
Article
Cell Biology
Kavitha Kurup, Stephanie Matyi, Cory B. Giles, Jonathan D. Wren, Kenneth Jones, Aaron Ericsson, Daniel Raftery, Lu Wang, Daniel Promislow, Arlan Richardson, Archana Unnikrishnan
Summary: The study shows that calorie restriction has significant effects on the microbiome, fecal metabolome, and colon transcriptome of mice, maintaining a more youthful state of the gastrointestinal system, which may lead to a more diverse and youthful microbiome.
Article
Biochemistry & Molecular Biology
Tracy A. Bedrosian, Judith Houtman, Juan Sebastian Eguiguren, Saeed Ghassemzadeh, Nicole Rund, Nicole M. Novaresi, Lauren Hu, Sarah L. Parylak, Ahmet M. Denli, Lynne Randolph-Moore, Takashi Namba, Fred H. Gage, Tomohisa Toda
Summary: The age-dependent decline of lamin B1 in adult neural stem/progenitor cells is associated with alterations in adult hippocampal neurogenesis and anxiety-like behavior in mice, indicating a role in stem cell aging and mood regulation.
Article
Nutrition & Dietetics
Margalida Torrens-Mas, Cayetano Navas-Enamorado, Devin Wahl, Andres Sanchez-Polo, Anna Picca, Jordi Oliver, Pilar Roca, Marta Gonzalez-Freire
Summary: “Calorie restriction (CR) is the only nutritional strategy proven to extend lifespan, prevent age-related diseases, and delay functional decline. The effects of CR when started early in life are not well understood. This study found that young female rats treated with CR showed similar expression patterns of key genes/pathways associated with healthy aging compared to old animals treated with CR, while in male rats these effects were reduced.”
Review
Cell Biology
Begun Erbaba, Ayca Arslan-Ergul, Michelle M. Adams
Summary: Caloric restriction is a non-genetic intervention that extends lifespan and healthspan by promoting cell survival, reducing cell proliferation, inducing stem cell quiescence, and preserving stem cell reserve for extreme needs. This protective mechanism induced by diet may involve downregulation of crucial cell cycle-related transcription activators and could offer new targets for treatments against neurodegenerative diseases.
AGEING RESEARCH REVIEWS
(2021)
Review
Biochemistry & Molecular Biology
Nastaran Daneshgar, Peter S. Rabinovitch, Dao-Fu Dai
Summary: mTOR signaling plays a crucial role in regulating cellular metabolism and aging by controlling key cellular processes such as protein synthesis, autophagy, and mitochondrial function. Dysregulation of mTOR signaling has been associated with various age-related pathologies, including heart failure and age-related cardiac dysfunction.
Article
Nutrition & Dietetics
Ana Teofilovic, Milos Vrataric, Natasa Velickovic, Danijela Vojnovic Milutinovic, Aleksandra Mladenovic, Milica Prvulovic, Ana Djordjevic
Summary: Late-onset calorie restriction has a positive impact on lipid metabolism but a negative impact on inflammation in the liver of old rats. It can restore age-related imbalance in lipid metabolism but does not improve expression of antioxidant enzymes and leads to progression of age-related inflammation in the liver.
FRONTIERS IN NUTRITION
(2022)
Article
Medicine, Research & Experimental
Cintia Maria Rodrigues, Talita Emanuela Domingues, Carina de Sousa Santos, Liliane Vanessa Costa-Pereira, Bruno Ferreira Mendes, Jousielle Marcia dos Santos, Karine Beatriz Costa, Gabriela Silva, Vinicius Lopes Cantuaria, Etel Rocha-Vieira, Marco Fabricio Dias-Peixoto, Kinulpe Honorato-Sampaio
Summary: Severe calorie restriction from birth shows cardioprotective effects in adult ovariectomized rats, improving cardiovascular function and reducing cardiac risk factors associated with menopause.
Review
Neurosciences
Laura Blasco-Chamarro, Isabel Farinas
Summary: Quiescence is a crucial mechanism for preserving the potential for cell proliferation at a later time. The molecular mechanisms regulating cell quiescence are complex and involve various signaling pathways, transcription factors, and epigenetic modifications. Understanding the regulation of the quiescent state is important for maintaining tissue homeostasis and exploring therapeutic strategies for adult neurogenesis.
Article
Neurosciences
Dmitry Velmeshev, Marco Magistri, Emilia Maria Cristina Mazza, Patrick Lally, Nathalie Khoury, Evan Ross D'Elia, Silvio Bicciato, Mohammad Ali Faghihi
MOLECULAR NEUROBIOLOGY
(2020)
Article
Genetics & Heredity
Maria Jesus Herrero, Dmitry Velmeshev, David Hernandez-Pineda, Saarthak Sethi, Shawn Sorrells, Payal Banerjee, Catherine Sullivan, Abha R. Gupta, Arnold R. Kriegstein, Joshua G. Corbin
Article
Biochemistry & Molecular Biology
Wei Huang, Aparna Bhaduri, Dmitry Velmeshev, Shaohui Wang, Li Wang, Catherine A. Rottkamp, Arturo Alvarez-Buylla, David H. Rowitch, Arnold R. Kriegstein
Article
Multidisciplinary Sciences
Jiasheng Zhang, Dmitry Velmeshev, Kei Hashimoto, Yu-Hsin Huang, Jeffrey W. Hofmann, Xiaoyu Shi, Jiapei Chen, Andrew M. Leidal, Julian G. Dishart, Michelle K. Cahill, Kevin W. Kelley, Shane A. Liddelow, William W. Seeley, Bruce L. Miller, Tobias C. Walther, Robert V. Farese, J. Paul Taylor, Erik M. Ullian, Bo Huang, Jayanta Debnath, Torsten Wittmann, Arnold R. Kriegstein, Eric J. Huang
Article
Biology
Anatoly Mikhailik, Tatyana Michurina, Krikor Dikranian, Stephen Hearn, Vladimir Maxakov, Saul S. Siller, Ken-Ichi Takemaru, Grigori Enikolopov, Natalia Peunova
Summary: This passage discusses the crucial role of nitric oxide in regulating the polarity and ciliary beat frequency of multiciliated cells in the airway, and how this impacts airway flow. It also suggests a potential explanation for the low levels of exhaled nitric oxide in ciliopathies due to deficiencies in NO availability in ciliated cells.
LIFE SCIENCE ALLIANCE
(2021)
Article
Cell Biology
Dmitry I. Maltsev, Kennelia A. Mellanson, Vsevolod V. Belousov, Grigori N. Enikolopov, Oleg V. Podgorny
Summary: The detection of synthetic thymidine analogues incorporated into replicating DNA during the S-phase of the cell cycle is a valuable methodology for evaluating proliferative activity. Recent studies have shown that different thymidine analogues exhibit similar labeling kinetics and clearance rates from blood serum, with a bioavailability time of approximately 1 hour in vivo. This finding is significant for the practical use of multiple S-phase labeling with various analogues.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sergey Shuvaev, Alexander Lazutkin, Roman Kiryanov, Konstantin Anokhin, Grigori Enikolopov, Alexei A. Koulakov
Summary: This study introduces an algorithm called CORGI for the registration of perinatal brains. The algorithm utilizes image preprocessing, attention-gated simulated annealing, and classical multidimensional scaling to improve the accuracy of registration. Tested on samples of perinatal mouse brains, the algorithm shows promising results and automates brain registration tasks with a short runtime, making it significant for studying brain development dynamics.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Yuechuan Xue, Zachary Gursky, Brittany Monte, Sunil Koundal, Xiaodan Liu, Hedok Lee, Tatyana Michurina, Kennelia A. Mellanson, Lucy Zhao, Alice Nemajerova, Kristopher T. Kahle, Ken-Ichi Takemaru, Grigori Enikolopov, Natalia Peunova, Helene Benveniste
Summary: This study investigated the impact of ciliopathies on CNS fluid homeostasis. The results showed that sustained glymphatic transport and impaired solute drainage via the cribriform plate to the nasal cavity were observed in models of ciliopathy, along with the occurrence of hydrocephalus. This research is important for understanding how ciliopathies contribute to disruption of CNS fluid homeostasis.
FLUIDS AND BARRIERS OF THE CNS
(2022)
Article
Cell Biology
Vashe Chandrakanthan, Prunella Rorimpandey, Fabio Zanini, Diego Chacon, Jake Olivier, Swapna Joshi, Young Chan Kang, Kathy Knezevic, Yizhou Huang, Qiao Qiao, Rema A. Oliver, Ashwin Unnikrishnan, Daniel R. Carter, Brendan Lee, Chris Brownlee, Carl Power, Robert Brink, Simon Mendez-Ferrer, Grigori Enikolopov, William Walsh, Berthold Gottgens, Samir Taoudi, Dominik Beck, John E. Pimanda
Summary: Chandrakanthan et al. have identified Mesp1-derived PDGFRA(+) stromal cells as aortic endothelial precursor cells that regulate the generation of long-term hematopoietic stem cells during development. This study provides a new potential tool for generating engraftable hematopoietic stem cells.
NATURE CELL BIOLOGY
(2022)
Article
Cell Biology
Anna Ivanova, Olesya Gruzova, Elizaveta Ermolaeva, Olga Astakhova, Sheed Itaman, Grigori Enikolopov, Alexander Lazutkin
Summary: This study examines the effects of thymidine analogs on adult hippocampal neurogenesis and perinatal somatic development of mice. The results show that thymidine analogs can effectively label cells but may have long-term effects on cell survival. Additionally, higher doses of thymidine analogs adversely affect somatic maturation and brain growth in mouse pups.
Article
Clinical Neurology
Tim Trobisch, Amel Zulji, Nikolas A. Stevens, Sophia Schwarz, Sven Wischnewski, Mikail Ozturk, Javier Perales-Paton, Maximilian Haeussler, Julio Saez-Rodriguez, Dmitry Velmeshev, Lucas Schirmer
Summary: In patients with multiple sclerosis (MS), it was found that the transcriptomic characteristics of astrocytes overlap across different regions, and different reactive glial cell types may exacerbate or ameliorate pathology.
ACTA NEUROPATHOLOGICA
(2022)
Article
Cell Biology
Sheed Itaman, Grigori Enikolopov, Oleg V. Podgorny
Summary: Tissue-specific somatic stem cells are characterized by their ability to be in a state of prolonged reversible cell cycle arrest. Maintaining a balance between cell quiescence and division is crucial for tissue homeostasis. However, detecting de novo dividing cells in quiescent stem cells has been challenging. In this study, a novel method based on double labeling with thymidine analogues was presented and experimentally verified to detect de novo dividing stem cells in situ.
Article
Multidisciplinary Sciences
Dmitry Velmeshev, Yonatan Perez, Zihan Yan, Jonathan E. Valencia, David R. Castaneda-Castellanos, Li Wang, Lucas Schirmer, Simone Mayer, Brittney Wick, Shaohui Wang, Tomasz Jan Nowakowski, Mercedes Paredes, Eric J. Huang, Arnold R. Kriegstein
Summary: In this study, we analyzed over 700,000 single-nucleus RNA sequencing profiles to identify lineage-specific programs and gene expression networks that regulate the development of specific cortical cell types. By intersecting our results with genetic risk factors, we identified the vulnerable cell types and lineages associated with different brain disorders, particularly autism. Our study provides insights into the molecular progression of cortical lineages during human development.
