期刊
EUROPEAN JOURNAL OF NEUROSCIENCE
卷 37, 期 1, 页码 1-9出版社
WILEY-BLACKWELL
DOI: 10.1111/ejn.12037
关键词
behavioral sensitisation; dendritic spines; DiI; diolistic labeling; medium spiny neurons; nucleus accumbens
资金
- National Institutes of Health [DA00436, DA14241, AA017922, AA010983]
The adducin family of proteins associates with the actin cytoskeleton in a calcium-dependent manner. Beta adducin (beta Add) is involved in synaptic plasticity in the hippocampus; however, the role of beta Add in synaptic plasticity in other brain areas is unknown. Using diolistic labeling with the lipophilic dye DiI, we found that the density of mature mushroom-shaped spines was significantly decreased in the nucleus accumbens (NAc) in brain slices from beta Add-knockout (KO) mice as compared to their wildtype (WT) siblings. The effect of 10 days of daily cocaine (15 mg/kg) administration on NAc spine number and locomotor behavior was also measured in beta Add WT and KO mice. As expected, there was a significant increase in overall spine density in NAc slices from cocaine-treated WT mice at this time-point; however, there was a greater increase in the density of mushroom spines in beta Add-KO animals following chronic cocaine administration than in WT. In addition, beta Add-KO mice showed elevated locomotor activity in response to cocaine treatment compared to WT siblings. These results indicate that beta Add is required for stabilising mature spines under basal conditions in the NAc, but that lack of this protein does not prevent synaptic remodeling following repeated cocaine administration. In addition, these data are consistent with previous studies suggesting that beta Add may normally be involved in stabilising spines once drug- or experience-dependent remodeling has occurred.
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