Motor and non-motor projections from the cerebellum to rostrocaudally distinct sectors of the dorsal premotor cortex in macaques

In the caudal part of the dorsal premotor cortex of macaques (area F2), both anatomical and physiological studies have identified two rostrocaudally separate sectors. The rostral sector (F2r) is located medial to the genu of the arcuate sulcus, and the caudal sector (F2c) is located lateral to the superior precentral dimple. Here we examined the sites of origin of projections from the cerebellum to F2r and F2c. We applied retrograde transsynaptic transport of a neurotropic virus, CVS-11 of rabies virus, in macaque monkeys. Three days after rabies injections into F2r or F2c, neuronal labeling was found in the deep cerebellar nuclei mainly of the contralateral hemisphere. After the F2r injection, labeled cells were distributed primarily in the caudoventral portion of the dentate nucleus, whereas cells labeled after the F2c injection were distributed in the rostrodorsal portion of the dentate nucleus, and in the interpositus and fastigial nuclei. Four days after rabies injections, Purkinje cells were densely labeled in the lateral part of the cerebellar cortex. After the F2r injection, Purkinje cell labeling was confined to Crus I and II, whereas the labeling seen after the F2c injection was located broadly from lobules III to VIII, including Crus I and II. These results have revealed that F2c receives inputs from broader areas of the cerebellum than F2r, and that distinct portions of the deep cerebellar nuclei and the cerebellar cortex send major projections to F2r and F2c, suggesting that F2c and F2r may be under specific influences of the cerebellum.