Article
Psychiatry
Javier Ballester, Anne K. Baker, Ilkka K. Martikainen, Vincent Koppelmans, Jon-Kar Zubieta, Tiffany M. Love
Summary: This study found that chronic pain patients at high risk for opioid misuse showed higher baseline MOR availability in the right amygdala, while patients at low risk for opioid misuse showed less pain-induced activation of MOR-mediated, endogenous opioid neurotransmission in the nucleus accumbens.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Chemistry, Analytical
Michelle Hadad, Nadine Hadad, Alexander G. Zestos
Summary: In this study, a method for measuring cortisol using carbon fiber microelectrodes and fast-scan cyclic voltammetry was developed. This method showed high sensitivity, high spatiotemporal resolution, and biocompatibility, and it was able to detect cortisol stably. Its application will contribute to a better understanding of the physiological importance of cortisol.
Article
Chemistry, Physical
Karla Caroline de Freitas Araujo, Emily Cintia Tossi de Araujo Costa, Danyelle Medeiros de Araujo, Elisama V. Santos, Carlos A. Martinez-Huitle, Pollyana Souza Castro
Summary: In the development of electrochemical sensors, carbon micro-structured or micro-materials have been widely used as supports/modifiers to improve the performance of bare electrodes. However, no attempts for electroanalytical determination of caffeine with CF microelectrode have been reported in the literature. Therefore, a homemade CF-microelectrode was fabricated and used to determine caffeine in soft beverage samples.
Review
Chemistry, Analytical
Cailing He, Mengdan Tao, Chenxi Zhang, Yifang He, Wei Xu, Yan Liu, Wanying Zhu
Summary: Dopamine is an essential neurotransmitter in the central nervous system and its abnormal metabolism is related to various diseases. Electrochemical sensing technology, particularly with microelectrodes, is recognized as the most effective method for measuring neurotransmitters in vivo. This article reviews recent applications and research on microelectrodes for dopamine detection in vivo, highlighting the advantages of high sensitivity and spatial resolution.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Electrochemistry
Chase Stucky, Michael A. Johnson
Summary: In this study, it was found that three different SSRIs significantly decrease the oxidation current of serotonin when using the commonly used Jackson waveform. Coating carbon-fiber microelectrodes with Nafion further reduces the current and increases response times. To address fouling issues, an extended serotonin waveform was used, which alleviated the decrease in current, indicating reduced electrode fouling.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Boyu Zhu, Xinru Li, Lihang Zhu, Min Qi, Jiayi Cao, Lin Zhou, Bin Su
Summary: This study investigates the changes in brain glucose levels of early diabetic mice using in vivo electrochemical measurement. The research suggests that diabetes may downregulate the activity of glucose transporter in the brain, leading to a decrease in brain glucose uptake.
Article
Chemistry, Multidisciplinary
A. Chathuri De Alwis, J. Dylan Denison, Ruby Shah, Gregory S. McCarty, Leslie A. Sombers
Summary: Carbon fiber microelectrodes are commonly used for real-time monitoring of exocytosis events. This study compared the performance of 10-μm and 34-μm diameter microelectrodes and found that the larger electrode was more sensitive to certain molecules and covered the entire cell, leading to better detection of exocytosis events and quantal size.
Article
Sport Sciences
Yu Zhou, Graham Finlayson, Xudong Liu, Qichen Zhou, Tianze Liu, Chenglin Zhou
Summary: The study examined the effects of acute moderate-intensity dance and aerobic exercise on drug craving, appetite, prefrontal neural activation to food cues, and food reward in women with methamphetamine dependence. Results showed that both interventions reduced subjective craving for drugs and increased implicit wanting and relative preferences for high-calorie savory foods. The findings suggest that moderate-intensity exercise can be used as a therapeutic intervention to restore the balance between drug and nondrug rewards.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2021)
Article
Biophysics
Elisa Castagnola, Elaine M. Robbins, Daniela D. Krahe, Bingchen Wu, May Yoon Pwint, Qun Cao, Xinyan Tracy Cui
Summary: Researchers have developed an implantable and flexible sensor that can measure tonic serotonin levels in multiple locations within the mouse hippocampus for an extended period of time. The sensor is highly sensitive, resistant to fouling, and selective for serotonin measurement.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Immunology
Ruben Garcia-Cabrerizo, Thaisa Barros-Santos, David Campos, John F. Cryan
Summary: The gut microbiota plays a crucial role in maintaining physiological balance and immunity, and is associated with addiction. Animal studies have shown a link between reward and social processes, which can be influenced by the gut microbiota, impacting neurodevelopment and social behaviors across different species. Depletion of the gut microbiota alters reward responses to drug and social stimuli, with a decrease in drug reward and an increase in social reward. Additionally, depleting the gut microbiota in the presence of positive social stimuli attenuates cocaine reward. These findings suggest that targeting the microbiota and enhancing social behavior could be beneficial in reducing harm from drug use.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Review
Chemistry, Physical
Stephane Marinesco
Summary: Chemical transmission between neurosecretory cells is a central biological phenomenon that can be studied using electrochemical techniques. Carbon fiber microelectrodes are commonly used to detect neurotransmitters and can be modified for specific and sensitive in situ neurotransmitter detection, making them an essential tool for analyzing living cells and tissues.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Review
Psychiatry
Elif Engin
Summary: Benzodiazepines have been widely used for many years, but their side effects and abuse potential have led to the search for alternatives. The development of subtype-selective benzodiazepine-like drugs is currently being explored, although it is uncertain whether these strategies can reduce misuse and abuse potential.
FRONTIERS IN PSYCHIATRY
(2023)
Article
Chemistry, Analytical
Ziyue Sun, Hongli Zhao, Kaicha Chen, Fangfang Zhou, Minbo Lan
Summary: In this study, a carbon fiber microelectrode (CFME) coated with an ERGO-CNT network was used as a substrate to support dendrite PtPd nanoclusters, and an enzyme-free O2 center dot- electrochemical sensor (PtPd@ERGO-CNT-CFME) was prepared based on this strategy. The as-constructed sensor exhibited outstanding detection ability towards O2 center dot-, attributed to the loading and dispersing abilities of the substrate and the electrochemical activity of the dendrite PtPd alloy. The sensor showed a wide-linear range, high sensitivity, and low detection limit, and was successfully applied for O2 center dot- detection in biological samples.
MICROCHEMICAL JOURNAL
(2023)
Review
Psychiatry
Fabrizio Schifano, Stefania Chiappini, Andrea Miuli, Alessio Mosca, Maria Chiara Santovito, John M. Corkery, Amira Guirguis, Mauro Pettorruso, Massimo Di Giannantonio, Giovanni Martinotti
Summary: This study analyzed published clinical data on over-the-counter (OTC) drug misuse, finding that the issue is widespread and popular worldwide, with vulnerable populations including adolescents and young adults. However, the true prevalence rates remain unknown due to a lack of appropriate monitoring systems. Healthcare professionals should remain vigilant and proactive in developing and implementing preventive measures for OTC misuse.
FRONTIERS IN PSYCHIATRY
(2021)
Article
Chemistry, Analytical
Tingting Wang, Lili Yang, Yuan Li, Changhao Bao, Minyi Tang, Xianju Huang, Han Cheng
Summary: Gold nanoparticles modified carbon fiber microelectrodes were prepared for the sensitive detection of neurotransmitters, showing advantages in the selective determination of positively charged substances. The high selectivity for dopamine in the presence of high concentrations of ascorbic acid using gold nanoparticles has practical significance for neurotransmitter studies in biological systems.
ANALYTICAL LETTERS
(2021)
Article
Biology
Yuliya Voskobiynyk, Jonathan R. Roth, J. Nicholas Cochran, Travis Rush, Nancy V. N. Carullo, Jacob S. Mesina, Mohammad Waqas, Rachael M. Vollmer, Jeremy J. Day, Lori L. McMahon, Erik D. Roberson
Article
Multidisciplinary Sciences
Katherine E. Savell, Jennifer J. Tuscher, Morgan E. Zipperly, Corey G. Duke, Robert A. Phillips, Allison J. Bauman, Saakshi Thukral, Faraz A. Sultan, Nicholas A. Goska, Lara Ianov, Jeremy J. Day
Article
Neurosciences
Morgan E. Zipperly, Faraz A. Sultan, Guan-En Graham, Andrew C. Brane, Natalie A. Simpkins, Nancy V. N. Carullo, Lara Ianov, Jeremy J. Day
Summary: Exposure to drugs of abuse leads to long-lasting transcriptional and epigenetic changes in brain reward circuits, contributing to addiction. The gene Gadd45b plays a crucial role in dopamine-dependent epigenetic regulation and cocaine reward, as demonstrated through pharmacological manipulations and gene knockout experiments.
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nancy V. N. Carullo, Robert A. Phillips, Rhiana C. Simon, Salomon A. Roman Soto, Jenna E. Hinds, Aaron J. Salisbury, Jasmin S. Revanna, Kendra D. Bunner, Lara Ianov, Faraz A. Sultan, Katherine E. Savell, Charles A. Gersbach, Jeremy J. Day
NUCLEIC ACIDS RESEARCH
(2020)
Article
Neurosciences
Philipp Mews, Erin S. Calipari, Jeremy Day, Mary Kay Lobo, Timothy Bredy, Ted Abel
Summary: The central goal of neuroscience research is to understand how experiences modify brain circuits for future adaptive behavior, involving complex interactions between circuit activity and gene regulation. Disruptions in this interplay are linked to debilitating psychiatric conditions, emphasizing the importance of studying how neural circuits and the epigenome cooperate to produce behavioral adaptation.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Skylar E. Davis, Jonathan R. Roth, Qays Aljabi, Ahmad R. Hakim, Katherine E. Savell, Jeremy J. Day, Andrew E. Arrant
Summary: Loss-of-function mutations in progranulin are a major genetic cause of frontotemporal dementia, with the neurotrophic and antiinflammatory effects of progranulin potentially contributing to this. The protective effects of progranulin against excitotoxicity are likely mediated through lysosomes, specifically through the autophagy-lysosomal pathway. Lysosome-targeted progranulin showed more consistent protective effects, indicating a mechanistic link between progranulin's lysosomal and neurotrophic effects.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Editorial Material
Neurosciences
Jennifer J. Tuscher, Jeremy J. Day
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Neurosciences
Nancy V. N. Carullo, Jenna E. Hinds, Jasmin S. Revanna, Jennifer J. Tuscher, Allison J. Bauman, Jeremy J. Day
Summary: Targeted genetic and epigenetic modification is crucial in molecular neuroscience to understand gene regulatory mechanisms underlying complex behaviors. While CRISPR-based systems have revolutionized genome editing, few tools exist for cell-specific CRISPR activation in neurons. This study presents a Cre-dependent CRISPRa system with improved efficiency and specificity, providing a versatile tool for gene regulation in different model systems.
Article
Neurosciences
L. J. McMeekin, K. L. Joyce, L. M. Jenkins, B. M. Bohannon, K. D. Patel, A. S. Bohannon, A. Patel, S. N. Fox, M. S. Simmons, J. J. Day, A. Kralli, D. K. Crossman, R. M. Cowell
Summary: This study identified ERRa as necessary for normal expression of PGC-1a-dependent genes in neurons, with a strong correlation in expression between ERRa and PGC-1a in the brain. In ERRa null mice, PGC-1a-dependent genes were reduced in multiple brain regions, leading to behavioral abnormalities without severe motor impairments seen in PGC-1a null mice.
Correction
Neurosciences
L. J. McMeekin, K. L. Joyce, L. M. Jenkins, B. M. Bohannon, K. D. Patel, A. S. Bohannon, A. Patel, S. N. Fox, M. S. Simmons, J. J. Day, A. Kralli, D. K. Crossman, R. M. Cowell
Article
Cell Biology
Robert A. Phillips, Jennifer J. Tuscher, Samantha L. Black, Emma Andraka, N. Dalton Fitzgerald, Lara Ianov, Jeremy J. Day
Summary: This study comprehensively defines the molecular architecture of the ventral tegmental area (VTA) by performing single-nucleus RNA sequencing on rat VTA cells. It identifies selective markers for dopamine and combinatorial neurons, reveals expression profiles for drug receptors, and demonstrates population-specific enrichment of gene sets linked to brain disorders.
Editorial Material
Neurosciences
Jeremy J. Day, Keri Martinowich
NEUROPSYCHOPHARMACOLOGY
(2023)
Meeting Abstract
Neurosciences
Jennifer Tuscher, Robert Philliips, Samantha Black, Lara Ianov, Jeremy J. Day
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Corey. G. G. Duke, Svitlana. V. V. Bach, Jasmin. S. S. Revanna, Faraz. A. A. Sultan, Nicholas. T. T. Southern, M. Natalie Davis, Nancy V. N. Carullo, Allison. J. J. Bauman, Robert. A. A. Phillips, Jeremy. J. J. Day
FRONTIERS IN GENOME EDITING
(2020)
Article
Neurosciences
Corey G. Duke, Katherine E. Savell, Jennifer J. Tuscher, Robert A. Phillips, Jeremy J. Day