期刊
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
卷 116, 期 9, 页码 1195-1204出版社
WILEY
DOI: 10.1002/ejlt.201300404
关键词
DNA and siRNA transfection; Lipopolyplex; Liposome; Nebulization; Polyethlyenimine
资金
- Deutsche Forschungsgemeinschaft [AI 24/6-1, AI 24/9-1]
- SMWK (Saxonian Ministry for Science and Art)
- Deutsche Krebshilfe [110184]
The delivery of nucleic acids into the lung is still one major bottleneck and relies on the development of efficient non-viral carriers that can be nebulized for their direct administration. Liposomal formulations, including those comprising different phospholipids, have been intensively studied. Likewise, polyethylenimines (PEI) have been widely explored due to their ability to protect nucleic acids from degradation and mediate cellular uptake and endosomal/lysosomal release. The combination of PEIs and liposomes is a particularly intriguing concept, leading to enhanced transfection efficiencies, lower cytotoxicities, and protection from aggregation. We have previously established lipopolyplexes, comprising PEI F25-LMW and the neutral phospholipid DPPC with various colipids. However, nebulization shear forces may be problematic for lipid formulations and require the testing of lipopolyplexes. In this paper, we demonstrate that DNA-or siRNA-containing PEI-based lipopolyplexes are indeed stable toward nebulization and retain their physicochemical integrity and biological activity. DNA transfection efficacies, but not siRNA knockdown, of certain lipopolyplexes are even enhanced upon nebulization, and nebulized lipopolyplexes also allow storage. This is due to the preservation of lipopolyplex size and zeta potential upon nebulization. More detailed analyses also reveal a dependence on the lipid, the medium used for preparation, the PEI and the nucleic acid.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据