期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 44, 期 4, 页码 1156-1169出版社
WILEY-BLACKWELL
DOI: 10.1002/eji.201343660
关键词
Endothelial cell migration; Exosomes; Integrin trafficking; Macrophages
类别
资金
- Future-based Technology Development Program through the National Research Foundation of Korea
- Ministry of Education, Science, and Technology [2009-0081760]
- National Research Foundation of Korea [2009-0081760] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Integrin trafficking, including internalization, recycling, and lysosomal degradation, is crucial for the regulation of cellular functions. Exosomes, nano-sized extracellular vesicles, are believed to play important roles in intercellular communications. This study demonstrates that exosomes released from human macrophages negatively regulate endothelial cell migration through control of integrin trafficking. Macrophage-derived exosomes promote internalization of integrin 1 in primary HUVECs. The internalized integrin 1 persistently accumulates in the perinuclear region and is not recycled back to the plasma membrane. Experimental results indicate that macrophage-derived exosomes stimulate trafficking of internalized integrin 1 to lysosomal compartments with a corresponding decrease in the integrin destined for recycling endosomes, resulting in proteolytic degradation of the integrin. Moreover, ubiquitination of HUVEC integrin 1 is enhanced by the exosomes, and exosome-mediated integrin degradation is blocked by bafilomycin A, a lysosomal degradation inhibitor. Macrophage-derived exosomes were also shown to effectively suppress collagen-induced activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase signaling pathway and HUVEC migration, which are both dependent on integrin 1. These observations provide new insight into the functional significance of exosomes in the regulation of integrin trafficking.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据