期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 43, 期 7, 页码 1896-1906出版社
WILEY
DOI: 10.1002/eji.201242792
关键词
CpG oligonucleotide; Dendritic cell; IRF-5; NF-kappa B; TLR9
类别
资金
- Intramural Research Program of the NIH, NCI [HHSN261200800001E]
- Department of Immunology, University of Washington
Synthetic oligonucleotides (ODN) expressing CpG motifs mimic the ability of bacterial DNA to trigger the innate immune system via TLR9. Plasmacytoid dendritic cells (pDCs) make a critical contribution to the ensuing immune response. This work examines the induction of antiviral (IFN-) and pro-inflammatory (IL-6) cytokines by CpG-stimulated human pDCs and the human CAL-1 pDC cell line. Results show that interferon regulatory factor-5 (IRF-5) and NF-B p50 are key co-regulators of IFN- and IL-6 expression following TLR9-mediated activation of human pDCs. The nuclear accumulation of IRF-1 was also observed, but this was a late event that was dependant on type 1 IFN and unrelated to the initiation of gene expression. IRF-8 was identified as a novel negative regulator of gene activation in CpG-stimulated pDCs. As variants of IRF-5 and IRF-8 were recently found to correlate with susceptibility to certain autoimmune diseases, these findings are relevant to our understanding of the pharmacologic effects of K ODN and the role of TLR9 ligation under physiologic, pathologic, and therapeutic conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据