Article
Virology
Leon C. W. Lin, Sarah N. Croft, Nathan P. Croft, Yik Chun Wong, Stewart A. Smith, Swee-Seong Tang, Anthony W. Purcell, David C. Tscharke
Summary: The study investigated the CD8(+) T cell priming pathways for Vaccinia virus (VACV) by engineering the virus to express inhibitors of antigen presentation. While the inhibitors had diverse impacts on CD8(+) T cell responses, unexpectedly, the direct priming of CD8(+) T cells by poxviruses was robust enough to withstand potent viral inhibitors of antigen presentation. This demonstrates the flexibility and robustness of immune processes in activating immune responses required to fight infection.
JOURNAL OF VIROLOGY
(2021)
Review
Biochemistry & Molecular Biology
Karolina Kot, Natalia Lanocha-Arendarczyk, Michal Ptak, Aleksandra Lanocha, Elzbieta Kalisinska, Danuta Kosik-Bogacka
Summary: This review discusses the biochemical and molecular mechanisms in kidneys infected with protozoan parasites like Leishmania spp., Plasmodium spp., Toxoplasma gondii, and Acanthamoeba spp. Pathomechanisms of Leishmania spp. and Plasmodium spp. infections have been deeply investigated, providing insight into novel treatments, while Toxoplasma gondii and Acanthamoeba spp. infections in the kidneys require further research to understand their mechanisms. Gaps in the pathophysiology of renal toxoplasmosis and acanthamoebiasis are highlighted, indicating the need for more studies in this area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Aleksandra Lanocha, Natalia Lanocha-Arendarczyk, Dominika Wilczynska, Barbara Zdziarska, Danuta Kosik-Bogacka
Summary: This study aimed to evaluate the frequency of gastrointestinal protozoan infection in patients with hematological malignancies undergoing intensive treatment and to determine the biological factors influencing intestinal parasite infection. The results showed a higher prevalence of parasitic infections in patients with hematological malignancies undergoing treatment.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Alexandra Linke, Hakan Cicek, Anne Mueller, Catherine Meyer-Schwesinger, Simon Melderis, Thorsten Wiech, Claudia Wegscheid, Julius Ridder, Oliver M. Steinmetz, Linda Diehl, Gisa Tiegs, Katrin Neumann
Summary: This study found that proximal tubular epithelial cells (PTECs) have the capacity for antigen cross-presentation, which can modulate the immune response in immune-mediated glomerular diseases, such as lupus nephritis.
Article
Immunology
Nitya Mohan, Kathrin Wellach, Ceren Oezerdem, Nisha Veits, Jonas D. Foerster, Sophia Foehr, Maria Bonsack, Angelika B. Riemer
Summary: This study investigates the effect of hypoxia on antigen presentation and T cell cytotoxicity in cervical cancer cells. The results show downregulation of E6 and E7 expression under hypoxic conditions, but intact antigen processing and presentation machinery. The cytotoxicity of T cells towards cervical cancer cells is affected by both epitope-specific and donor-specific factors.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Microbiology
Zhiteng Zhao, Xin Li, Nan Zhang, Jianhua Li, Na Zhao, Mengyao Gao, Xichen Zhang, Xiaocen Wang, Panpan Zhao, Lu Li, Min Sun, Lili Cao, Pengtao Gong
Summary: PPVs-based regulation of parasitic protozoa can provide a theoretical basis and direction for preventing and controlling parasitic protozoan diseases (PPDs), although the mechanisms of PPVs and their regulation are unclear. This review investigates the differences and unique properties of PPVs, focusing on Trichomonas vaginalis virus, Giardia lamblia virus, Leishmania RNA virus, and Cryptosporidium parvum virus 1. The triangular association between PPVs, parasitic protozoa, and hosts reveals the dual effects of PPVs on parasitic protozoa and hosts. These studies highlight the complexity of parasitic protozoa and suggest new pathways for using PPVs as tools to understand protozoal infection and treatment.
Article
Cell Biology
Ryosuke Tashiro, Kuniyasu Niizuma, Jun Kasamatsu, Yuko Okuyama, Sherif Rashad, Atsuo Kikuchi, Miki Fujimura, Shigeo Kure, Naoto Ishii, Teiji Tominaga
Summary: The study found that RNF213 plays a critical role in antigen uptake, processing, and presentation, with Rnf213-KO and Rnf213-KI mice experiments showing that RNF213 deficiency leads to decreased antigen uptake and processing capabilities, resulting in the inability to effectively activate antigen-specific T cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vita Golubovskaya, Hua Zhou, Feng Li, Robert Berahovich, Jinying Sun, Michael Valentine, Shirley Xu, Hizkia Harto, John Sienkiewicz, Yanwei Huang, Lijun Wu
Summary: This study focused on developing novel CS1 CAR-T cells and bispecific CS1-BCMA CAR-T cells for targeting multiple myeloma. The experimental results demonstrated that these cells effectively killed multiple myeloma cells, showing promise for future clinical trials.
Article
Biology
Fernando Y. Maeda, Jurriaan J. H. van Haaren, David B. Langley, Daniel Christ, Norma W. Andrews, Wenxia Song
Summary: Recognition of antigen by B-cell receptor results in localized plasma membrane permeabilization, triggering PM repair responses involving lysosomal exocytosis to facilitate antigen internalization and presentation. Higher affinity antigens cause more B-cell permeabilization and are efficiently presented to T-cells.
Article
Cell Biology
Jinyu Wang, Michela Manni, Anne Baerenwaldt, Ronja Wieboldt, Nicole Kirchhammer, Robert Ivanek, Michal Stanczak, Alfred Zippelius, David Koenig, Natalia Rodrigues Manutano, Heinz Laeubli
Summary: Interactions between sialylated glycans and Siglec receptors have been identified as a potential new immune checkpoint to improve anticancer immunity. In this study, it was found that cancer-associated dendritic cells (DCs) have a high expression of inhibitory Siglecs, which can impair their maturation states. Furthermore, removing these inhibitory Siglecs from DCs enhances their capability to prime antigen-specific T cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Lizhong Ding, Lu Sun, Melissa T. Bu, Yanjun Zhang, Lauren N. Scott, Robert M. Prins, Maureen A. Su, Melissa G. Lechner, Willy Hugo
Summary: Tumors of melanoma patients who respond to ICI or MAPKi therapies show increased T cell infiltration and IFNγ pathway activation. However, the rate of durable tumor control is higher with ICI therapy, indicating the presence of additional mechanisms beneficial for anti-tumor immunity in ICI responders.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Anita Tewari, Miglena G. G. Prabagar, Sophie L. L. Gibbings, Kavita Rawat, Claudia V. V. Jakubzick
Summary: Research has shown that monocytes in the lymph nodes play a critical role in regulating immune response, indirectly dampening antigen-specific cytotoxic T cell response induced by Poly I:C, exerting a braking function mediated by IL-10 production and induction of suppressor CD4(+) T cells. A triple-combination treatment consisting of anti-IL-10, tumor peptides, and Poly I:C in a metastatic melanoma model has been successful in reducing tumor growth compared to treatments with tumor peptides and Poly I:C alone.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Jimena Alvarez Freile, Yuzhu Qi, Lisa Jacob, Maria Franceskin Lobo, Harm Jan Lourens, Gerwin Huls, Edwin Bremer
Summary: Investigations into the strength of antigen-specific responses in vitro are crucial for early-phase research of immunotherapeutic approaches. This study presents a rapid luminescence-based method using HPV16 E7(11-20) peptide as a model antigen to evaluate MHC-dependent antigen-specific T cell responses in vitro. The method has important implications for assessing the impact of tumor microenvironment and immune checkpoint inhibitors, as well as accelerating the development of novel immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Ching-Lien Wu, Julien Caumartin, Giada Amodio, Francois Anna, Maria Loustau, Silvia Gregori, Pierre Langlade-Demoyen, Joel LeMaoult
Summary: Invariant Natural Killer T (iNKT) cells, when activated by exogenic glycolipid antigen, can release cytokines rapidly to enhance anti-tumor activity. The presence of the tolerogenic molecule HLA-G in humans may impact the function of iNKT cells, potentially affecting the outcome of clinical trials.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Marie de Bourayne, Sylvain Meunier, Samuel Bitoun, Evelyne Correia, Xavier Mariette, Herve Nozach, Bernard Maillere
Summary: By investigating the role of PEGylation in T-cell reactivity, it has been demonstrated that pegylation reduces antigen capture by dendritic cells, peptide presentation to T-cells, and T-cell priming.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Tony Yao, Patrick T. Rudak, Celine M. Laumont, Alex R. Michaud, Rasheduzzaman Rashu, Natasha N. Knier, Paula J. Foster, Hamish E. G. McWilliam, Jose A. Villadangos, Brad H. Nelson, Gabriel E. DiMattia, Trevor G. Shepherd, S. M. Mansour Haeryfar
Summary: Research on mucosa-associated invariant T (MAIT) cells in epithelial ovarian cancer (EOC) reveals their low frequency in tumors but potential immunomodulatory and cytotoxic properties. In a mouse model, MAIT cells showed IL-17A and IFN-gamma production in the peritoneal cavity but lost these functions with tumor progression. Ascitic MAIT cells in EOC patients exhibit inducible effector functions, suggesting potential modifications for future immunotherapeutic strategies.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Review
Immunology
Thiago M. Steiner, William R. Heath, Irina Caminschi
Summary: This review summarizes the mounting evidence that conventional dendritic cells (cDC) can also present native antigen to B cells besides priming T cells, supporting the view that both cDC1 and cDC2 are capable of initiating humoral responses provided antigen is captured by relevant surface receptors attuned to this process. Such understanding is fundamental for the development of innovative humoral vaccination approaches.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Cell Biology
Rebecca Seneviratna, Samuel J. Redmond, Hamish E. G. McWilliam, Rangsima Reantragoon, Jose A. Villadangos, James McCluskey, Dale Godfrey, Nicholas A. Gherardin
Summary: Recent studies have shown that MR1-restricted T cells can react to a variety of antigens, including tumor cells and tumor-derived self-antigens. By using TCR gene transfer and engineered MR1-expressing antigen-presenting cells, researchers further investigated the reactivity of MR1-restricted TCRs and found differential dependence on MR1 position and competitive inhibition by ligands. However, TCR-expressing reporter cell lines failed to fully replicate the previously reported tumor specificity, suggesting the importance of accessory molecules in MR1-dependent tumor reactivity.
IMMUNOLOGY AND CELL BIOLOGY
(2022)
Article
Immunology
Maximilien Evrard, Erica Wynne-Jones, Changwei Peng, Yu Kato, Susan N. Christo, Raissa Fonseca, Simone L. Park, Thomas N. Burn, Maleika Osman, Sapna Devi, Jerold Chun, Scott N. Mueller, George Kannourakis, Stuart P. Berzins, Daniel G. Pellicci, William R. Heath, Stephen C. Jameson, Laura K. Mackay
Summary: The migratory receptor S1PR5 plays a critical role in T cell infiltration and emigration from peripheral organs, and is specifically downregulated in tissue-resident memory T (TRM) cells. The induction of S1pr5 relies on the transcription factors T-bet and ZEB2, and local TGF-beta signaling is necessary for the coordinated downregulation of Tbx21, Zeb2, and S1pr5. S1PR5-mediated control of tissue residency is conserved across innate and adaptive immune compartments.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Immunology
Shivam K. Purohit, Carolyn Samer, Hamish E. G. McWilliam, Renee Traves, Megan Steain, Brian P. McSharry, Paul R. Kinchington, David C. Tscharke, Jose A. Villadangos, Jamie Rossjohn, Allison Abendroth, Barry Slobedman
Summary: This study demonstrates that varicella zoster virus suppresses the expression of antigen presentation molecule MR1, highlighting the intricate temporal relationship between infection and ligand availability. The study also suggests that VZV likely encodes multiple viral genes targeting MR1.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Patrick Schriek, Alan C. Ching, Nagaraj S. Moily, Jessica Moffat, Lynette Beattie, Thiago M. Steiner, Laine M. Hosking, Joshua M. Thurman, V. Michael Holers, Satoshi Ishido, Mireille H. Lahoud, Irina Caminschi, William R. Heath, Justine D. Mintern, Jose A. Villadangos
Summary: Marginal zone B cells acquire dendritic cell-like properties through the trogocytic acquisition of MHC II-C3 complexes from conventional dendritic cells.
Article
Critical Care Medicine
Tanguy Chaumette, Raphael Cinotti, Alice Molle, Pierre Solomon, Louise Castain, Cynthia Fourgeux, Hamish E. G. McWilliam, Barbara Misme-Aucouturier, Alexis Broquet, Cedric Jacqueline, Mickael Vourc'h, Delphine Fradin, Celine Bossard, Laurent David, Emmanuel Montassier, Cecile Braudeau, Regis Josien, Jose A. Villadangos, Karim Asehnoune, Celine Bressollette-Bodin, Jeremie Poschmann, Antoine Roquilly
Summary: Brain injury induces immunosuppression, increasing the risk of viral reactivations and impairing neurological recovery. This study found that lung replication of herpesviridae is associated with poor recovery after brain injury, and a specific monocyte signature can predict the neurological outcome at 6 months.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Biochemical Research Methods
Vanta J. Jameson, Tina Luke, Yuting Yan, Angela Hind, Maximilien Evrard, Kevin Man, Laura K. Mackay, Axel Kallies, Jose A. Villadangos, Hamish E. G. McWilliam, Alexis Perez-Gonzalez
Summary: Understanding the impact of autofluorescence on signal resolution in cytometry is crucial for experimental design and data quality. This study provides mathematical predictions and empirical evidence to support the importance of autofluorescence extraction through full spectrum unmixing. The study also presents a novel method for isolating pure cell subset-specific autofluorescence profiles based on high dimensionality reduction algorithms.
Article
Multidisciplinary Sciences
Haiyin Liu, Kayla R. Wilson, Ashley M. Firth, Christophe Macri, Patrick Schriek, Annabelle B. Blum, Javiera Villar, Samuel Wormald, Mitch Shambrook, Bangyan Xu, Hui Jing Lim, Hamish E. G. McWilliam, Andrew F. Hill, Laura E. Edgington-Mitchell, Irina Caminschi, Mireille H. Lahoud, Elodie Segura, Marco J. Herold, Jose A. Villadangos, Justine D. Mintern
Summary: This study reveals the critical role of ubiquitin-like protein 3 (UBL3) in the trafficking process controlled by MARCH1. UBL3 has wide-ranging immunological consequences and is essential for immune responses.
NATURE COMMUNICATIONS
(2022)
Article
Cardiac & Cardiovascular Systems
Mickael Vourc'h, Antoine Roquilly, Adrien Foucher, Christelle Retiere, Fanny Feuillet, Sapna Devi, Hamish E. G. McWilliam, Cecile Braudeau, Geraldine Bourreille, Abderrahman Hachani, Dermot O'Kane, Scott N. Mueller, Joseph Ischia, Jean-Christian Roussel, Jean-Christophe Rigal, Regis Josien, Bertrand Rozec, Jose A. Villadangos, Karim Asehnoune
Summary: This study found that transfusion of packed red blood cells with high levels of MRP_14 may increase the incidence of AKI after cardiac surgery. Higher concentrations of MRP_14 were found in packed red blood cells from female donors or prepared by whole blood filtration.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2022)
Article
Cell Biology
Marine Gros, Elodie Segura, Derek C. Rookhuizen, Blandine Baudon, Sandrine Heurtebise-Chretien, Nina Burgdorf, Mathieu Maurin, Eugene A. Kapp, Richard J. Simpson, Patrycja Kozik, Jose A. Villadangos, Mathieu J. M. Bertrand, Marianne Burbage, Sebastian Amigorena
Summary: Despite its importance in immune responses, the molecular pathways underlying antigen cross-presentation are not fully understood. This study reveals that membrane repair plays a crucial role in containing antigen export to the cytosol and cross-presentation in conventional dendritic cells (cDCs).
Article
Cell Biology
Hui Jing Lim, Jacinta M. Wubben, Cristian Pinero Garcia, Sebastian Cruz-Gomez, Jieru Deng, Jeffrey Y. W. Mak, Abderrahman Hachani, Regan J. Anderson, Gavin F. Painter, Jesse Goyette, Shanika L. Amarasinghe, Matthew E. Ritchie, Antoine Roquilly, David P. Fairlie, Katharina Gaus, Jamie Rossjohn, Jose A. Villadangos, Hamish E. G. McWilliam
Summary: MR1 is a conserved microbial immune-detection system in mammals that presents antigens to specific lymphocytes, contributing to host defense and tissue repair. This study reveals that human MR1 interacts with the endocytic adaptor protein 2 (AP2) complex to regulate its internalization from the cell surface, thus controlling antigen presentation and microbial metabolic detection.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Immunology
Caroline L. Ashley, Brian P. McSharry, Hamish E. G. McWilliam, Richard J. Stanton, Ceri A. Fielding, Rommel A. Mathias, David P. Fairlie, James McCluskey, Jose A. Villadangos, Jamie Rossjohn, Allison Abendroth, Barry Slobedman
Summary: This study reveals that human cytomegalovirus (HCMV) inhibits the MR1 pathway and disrupts the MR1:MAIT cell axis through the viral protein gpUS9. The interaction between this virus and MAIT cells in the context of viral infection is not well characterized.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Patrick Schriek, Jose A. Villadangos
Summary: Antigen-presenting cells capture or synthesize antigens and present them on their plasma membrane through major histocompatibility complex (MHC) molecules. Trogocytosis is a mechanism where cells acquire fragments from other cells and incorporate them into their own plasma membrane, including intact antigens and MHC molecules. Trogocytosis expands cellular immunological functions with both beneficial and deleterious consequences.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Immunology
Mbaya Ntita, Shin-Ichi Inoue, Jiun-Yu Jian, Ganchimeg Bayarsaikhan, Kazumi Kimura, Daisuke Kimura, Mana Miyakoda, Eriko Nozaki, Takuya Sakurai, Daniel Fernandez-Ruiz, William R. Heath, Katsuyuki Yui
Summary: The differences in Plasmodium-specific CD4(+) T-cell responses between PbA- and Pcc-infected mice were associated with the difference in type I interferon production during the early phase of the infection. In PbA-infected mice, there was a higher response to type I interferon and a lower proportion of Th1 cells compared to Pcc-infected mice.
INTERNATIONAL IMMUNOLOGY
(2022)