期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 39, 期 8, 页码 2215-2223出版社
WILEY
DOI: 10.1002/eji.200939209
关键词
Chemokines; DC; Human; Transcription factors
类别
资金
- Arthritis Research Campaign
- Chiara Gasparini received a European Commission Marie Curie Individual Fellowship [MCFI-2000-02191]
DC, when fully matured are the APC best able to activate naive T cells. Recently, we demonstrated using adenoviruses overexpressing I kappa B alpha and proteosome inhibitors that NF-kappa B is involved in DC activation, but the role of the individual subunits is still not clear. We investigated the role of the NF-kappa B subunits RelB and p50 in human DC activation using adenoviral vectors expressing RelB or p50. Nuclear RelB, in the form of RelB/p50, was active only in DC infected with both viruses, this induced the production of the soluble homeostatic chemokine CCL19, but not other homeostatic chemokines, particularly in LPS-matured DC. However, RelB/p50 did not affect the expression of costimulatory and antigen-presenting molecules, and increased the allogeneic mixed lymphocyte reaction only in LPS-matured DC. This enhanced mixed lymphocyte reaction is most likely due to enhanced CCL19 production, which sustains the interaction between mature DC and naive T cells. In conclusion, we demonstrated that RelB/p50 was active only in DC expressing both RelB and p50, and induced CCL19 production, but not DC maturation.
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