Review
Biochemistry & Molecular Biology
Se-Jin Kim, Elham Karamooz
Summary: This review discusses the current understanding of MR1 and HLA-E antigen presentation in the context of Mycobacterium tuberculosis infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Tejpal Gill, James T. Rosenbaum
Summary: Spondyloarthritis (SpA) is a group of immune mediated inflammatory diseases strongly associated with the MHC class I molecule, HLA-B27. Although various hypotheses have been proposed to explain the HLA-B27 and disease association, the exact mechanisms underlying disease pathogenesis remain unclear. Evidence suggests that diseases related to HLA-B27 may have a microbial pathogenesis, and the influence of microbes on disease development is significant.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Denis Wakefield, Daniel Clarke, Peter McCluskey
Summary: Over the past 5 years, there has been steady progress in understanding the pathogenesis, clinical features, and treatment of acute anterior uveitis (AU). Large gene wide association studies have confirmed AU as a polygenic disease with overlaps with other conditions. Recent research has shown the importance of the microbiome in AU, with evidence suggesting that HLA B27 may influence gut microbiome composition. Clinical investigations have confirmed the typical features of AU and the value of immunosuppressive treatments.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: HLA molecules play critical roles in the adaptive immune system by presenting small peptides to signal cell health status to the immune system. This study investigated the adaptive response of a B lymphoblastic cell line to high temperature treatment, revealing potential preparations for immune-like responses in the absence of invading pathogenic peptides. The findings suggest intriguing temperature-sensitive adaptations in this particular B cell line.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Xinbo Yang, Lee I. I. Garner, Ivan V. V. Zvyagin, Michael A. A. Paley, Ekaterina A. A. Komech, Kevin M. M. Jude, Xiang Zhao, Ricardo A. A. Fernandes, Lynn M. M. Hassman, Grace L. L. Paley, Christina S. S. Savvides, Simon Brackenridge, Max N. N. Quastel, M. M. Chudakov, Paul Bowness, Wayne M. M. Yokoyama, Andrew J. J. McMichael, Geraldine M. M. Gillespie, K. Christopher Garcia
Summary: The human leucocyte antigen B*27 (HLA-B*27) is strongly associated with inflammatory diseases of the spine and pelvis and the eye. The mechanism by which HLA-B*27 facilitates disease remains unknown, but it may involve the presentation of pathogenic peptides to CD8(+) T cells. In this study, orphan T cell receptors (TCRs) expressing a disease-associated public beta-chain variable region-complementary-determining region 3 beta (BV9-CDR3 beta) motif were isolated from blood and synovial fluid T cells from individuals with ankylosing spondylitis (AS) and from the eye in individuals with acute anterior uveitis (AAU). These TCRs showed consistent alpha-chain variable region (AV21) chain pairing and were clonally expanded in the joint and eye. Using peptide libraries, self-peptides and microbial peptides that activated the AS- and AAU-derived TCRs were identified. Structural analysis revealed that TCR cross-reactivity for peptide-MHC was rooted in a shared binding motif present in both self-antigens and microbial antigens that engages the BV9-CDR3 beta TCRs. These findings suggest that both microbial antigens and self-antigens could play a pathogenic role in HLA-B*27-associated disease.
Review
Cell Biology
Irina A. Ishina, Maria Y. Zakharova, Inna N. Kurbatskaia, Azad E. Mamedov, Alexey A. A. Belogurov Jr, Alexander G. G. Gabibov
Summary: Antigen presentation by MHC-II is crucial for immune response and self-antigen tolerance. Certain MHC-II alleles are associated with autoimmune diseases. The process of MHC-II-mediated presentation plays a significant role in understanding the mechanisms of autoimmune diseases and the protective effect of certain MHC-II alleles.
Article
Biology
Maren Kasper, Michael Heming, David Schafflick, Xiaolin Li, Tobias Lautwein, Melissa Meyer zu Horste, Dirk Bauer, Karoline Walscheid, Heinz Wiendl, Karin Loser, Arnd Heiligenhaus, Gerd Meyer zu Hoerste
Summary: Uveitis is a group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. HLA-B27-positive uveitis displays a unique pattern of ocular leukocyte infiltration with a prominent presence of plasmacytoid and classical dendritic cells. These cells play a central role in predicted local cell-cell communication within the eye, suggesting their importance in HLA-B27-associated inflammation.
Article
Oncology
Marion Grard, Mohamed Idjellidaine, Atousa Arbabian, Camille Chatelain, Laurine Berland, Chantal Combredet, Soizic Dutoit, Sophie Deshayes, Virginie Dehame, Nathalie Labarriere, Delphine Fradin, Nicolas Boisgerault, Christophe Blanquart, Frederic Tangy, Jean-Francois Fonteneau
Summary: Antitumor virotherapy uses oncolytic viruses to induce tumor cell lysis and stimulate the antitumor immune response. In this study, a modified measles virus expressing the cancer/testis antigen NY-ESO-1 showed similar oncolytic activity to the wild-type virus but induced faster cell lysis. The modified virus also successfully induced NY-ESO-1 expression and presentation in melanoma cells and dendritic cells, leading to the activation of NY-ESO-1-specific T cells.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Immunology
Hesham ElAbd, Petra Bacher, Andreas Tholey, Tobias L. Lenz, Andre Franke
Summary: The HLA proteins play a crucial role in adaptive immunity by presenting self and foreign peptides to T cells. Genetic variation in the HLA region is associated with many complex diseases, highlighting the importance of specific HLA-presented peptides in disease etiology. Identifying the immunopeptidome, the set of peptides presented by an individual's HLA proteins in vivo, has garnered increasing attention for various reasons, such as cancer immunotherapy, vaccine development, and understanding autoimmunity. Despite significant progress, there are still unanswered questions and challenges in peptide presentation analysis and T cell immunogenicity. Addressing these challenges would enhance our understanding of disease etiology and lead to advancements in vaccine engineering and immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Ophthalmology
James Wawrzynski, Ana Rodriguez Martinez, Dorothy Ann Thompson, Dipak Ram, Richard Bowman, Rebecca Whiteley, Chin Gan, Louise Harding, Amanda Mortensen, Philippa Mills, Paul Gissen, Robert H. Henderson
Summary: Intravitreal rhTPP1 appears to be a safe and effective treatment for CLN2 related retinopathy, and early treatment is more likely to be efficacious.
Article
Medicine, General & Internal
Yimei Ding, Luan Xue
Summary: This study compared the clinical manifestations, imaging features, and laboratory inspection between HLA-B27 negative and HLA-B27 positive patients with Reactive arthritis (ReA). It found no significant difference in imaging features or laboratory inspection between the two groups, but there was a difference in the joints involved.
Article
Ophthalmology
Douglas A. Jabs, Nisha R. Acharya, Soon-Phaik Chee, Debra Goldstein, Peter McCluskey, Philip Murray, Neal Oden, Alan G. Palestine, James T. Rosenbaum, Jennifer E. Thorne, Brett E. Trusko
Summary: This study aimed to establish classification criteria for spondyloarthritis/HLA-B27-associated anterior uveitis using machine learning, achieving high accuracy in both training and validation sets. Key criteria for this condition were identified, with a low misclassification rate, indicating their potential utility in clinical and translational research.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Immunology
Nan Sheng, Yingying Gao, Hui Li, Wenwen Wang, Linyu Geng, Bo Zhang, Qiang Huang, Xueqin Wang, Lingyun Sun
Summary: This study explored the associations between several single nucleotide polymorphisms (SNPs) in the coding gene of tumor necrosis factor alpha (TNFa) and ankylosing spondylitis (AS) risk. The results showed that the T allele of rs1799724 was associated with increased AS risk, while the A allele of rs361525 was associated with decreased AS risk. Moreover, the contributions of rs1799724 and rs361525 to AS risk were dependent on HLA-B27 status.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
James T. Rosenbaum, Michael H. Weisman, Hedley Hamilton, Cassie Shafer, Elin Aslanyan, Richard A. Howard, Kimberly Ogle, John D. Reveille, Kevin L. Winthrop, Dongseok Choi
Summary: HLA-B27 positive patients with axial spondyloarthritis (AxSpA) tend to have lower disease activity compared to HLA-B27 negative patients, as indicated by higher BASDAI scores. HLA-B27 positive patients with mild back pain are more likely to be diagnosed with AxSpA, suggesting a cognitive bias introduced by the HLA-B27 test.
SCIENTIFIC REPORTS
(2021)
Review
Immunology
Danillo G. Augusto, Jill A. Hollenbach
Summary: The extraordinary variation of HLA molecules is critical for the immune response in COVID-19, but still not fully understood. Studies have found associations between HLA variants and different outcomes of COVID-19, as well as differential immune responses against SARS-CoV-2. Mutations in viral strains, particularly in the Spike protein, affect the affinity between mutant peptides and HLA molecules. Understanding the impact of this variation on T-cell responses is crucial for comprehending the immunogenic mechanisms.
CURRENT OPINION IN IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Miguel Marcilla, Inaki Alvarez, Antonio Ramos-Fernandez, Manuel Lombardia, Alberto Paradela, Juan Pablo Albar
JOURNAL OF PROTEOME RESEARCH
(2016)
Article
Biochemical Research Methods
Adab Alpizar, Fabio Marino, Antonio Ramos-Fernandez, Manuel Lombardia, Anita Jeko, Florencio Pazos, Alberto Paradela, Cesar Santiago, Albert J. R. Heck, Miguel Marcilla
MOLECULAR & CELLULAR PROTEOMICS
(2017)
Correction
Multidisciplinary Sciences
Paula Lopez-Serra, Miguel Marcilla, Alberto Villanueva, Antonio Ramos-Fernandez, Anna Palau, Lucia Leal, Jessica E. Wahi, Fernando Setien-Baranda, Karolina Szczesna, Catia Moutinho, Anna Martinez-Cardus, Holger Heyn, Juan Sandoval, Sara Puertas, August Vidal, Xavier Sanjuan, Eva Martinez-Balibrea, Francesc Vinals, Jose C. Perales, Jesper B. Bramsem, Torben F. Orntoft, Claus L. Andersen, Josep Tabernero, Ultan McDermott, Matthew B. Boxer, Matthew G. Vander Heiden, Juan Pablo Albar, Manel Esteller
NATURE COMMUNICATIONS
(2016)
Article
Rheumatology
Pablo Guasp, Carlos Alvarez-Navarro, Patricia Gomez-Molina, Adrian Martin-Esteban, Miguel Marcilla, Eilon Barnea, Arie Admon, Jose A. Lopez de Castro
ARTHRITIS & RHEUMATOLOGY
(2016)
Article
Biochemical Research Methods
Elizabeth Guruceaga, Alba Garin-Muga, Gorka Prieto, Bartolome Bejarano, Miguel Marcilla, Consuelo Marin-Vicente, Yasset Perez-Riverol, J. Ignacio Casal, Juan Antonio Vizcaino, Fernando J. Corrales, Victor Segura
JOURNAL OF PROTEOME RESEARCH
(2017)
Article
Biochemical Research Methods
Jesus Mateos, Isabel Carneiro, Fernando Corrales, Felix Elortza, Alberto Paradela, Manuel Sanchez del Pino, Ibon Iloro, Miguel Marcilla, Maria Isabel Mora, Luz Valero, Sergio Ciordia, Veronica Fernandez, Maria Antonia Fortuno, Isabel Garcia-Sanchez, Rosario Martinez, Maria Angeles Munoz, Clara Rodriguez, Nieves Domenech
JOURNAL OF PROTEOMICS
(2017)
Article
Biochemical Research Methods
Vital Vialas, Nuria Colome-Calls, Joaquin Abian, Kerman Aloria, Gloria Alvarez-Llamas, Oreto Antunez, Jesus M. Arizmendi, Mikel Azkargorta, Silvia Barcelo-Batllori, Maria G. Barderas, Francisco Blanco, J. Ignacio Casal, Vanessa Casas, Carolina de la Torre, Eduardo Chicano-Galvez, Felix Elortza, Guadalupe Espadas, Josep M. Estanyol, Joaquin Fernandez-Irigoyen, Patricia Fernandez-Puente, Maria Jose Fidalgo, Manuel Fuentes, Marina Gay, Concha Gil, Alexandre Hainard, Maria Luisa Hernaez, Nieves Ibarrola, Arthur T. Kopylov, Antonio Lario, Juan Antonio Lopez, Maria Lopez-Lucendo, Miguel Marcilla, Anabel Marina-Ramirez, Gyorgy Marko-Varga, Luna Martin, Maria I. Mora, Esperanza Morato-Lopez, Javier Munoz, Maria Antonia Odena, Eliandre de Oliveira, Irene Orera, Ignacio Ortea, Carla Pasquarello, Kevin B. Ray, Melinda Rezeli, Isabel Ruppen, Eduard Sabido, Manuel M. Sanchez del Pino, Jaime Sancho, Enrique Santamaria, Jesus Vazquez, Marta Vilasecar, Fernando Vivanco, James J. Walters, Victor G. Zgoda, Fernando J. Corrales, Francesc Canals, Alberto Paradela
JOURNAL OF PROTEOMICS
(2017)
Article
Biochemistry & Molecular Biology
Wenguang Shao, Patrick G. A. Pedrioli, Witold Wolski, Cristian Scurtescu, Emanuel Schmid, Juan A. Vizcaino, Mathieu Courcelles, Heiko Schuster, Daniel Kowalewski, Fabio Marino, Cecilia S. L. Arlehamn, Kerrie Vaughan, Bjoern Peters, Alessandro Sette, Tom H. M. Ottenhoff, Krista E. Meijgaarden, Natalie Nieuwenhuizen, Stefan H. E. Kaufmann, Ralph Schlapbach, John C. Castle, Alexey I. Nesvizhskii, Morten Nielsen, Eric W. Deutsch, David S. Campbell, Robert L. Moritz, Roman A. Zubarev, Anders Jimmy Ytterberg, Anthony W. Purcell, Miguel Marcilla, Alberto Paradela, Qi Wang, Catherine E. Costello, Nicola Ternette, Peter A. van Veelen, Cecile A. C. M. van Els, Albert J. R. Heck, Gustavo A. de Souza, Ludvig M. Sollid, Arie Admon, Stefan Stevanovic, Hans-Georg Rammensee, Pierre Thibault, Claude Perreault, Michal Bassani-Sternberg, Ruedi Aebersold, Etienne Caron
NUCLEIC ACIDS RESEARCH
(2018)
Article
Multidisciplinary Sciences
Begona Sot, Alejandra Rubio-Munoz, Ahudrey Leal-Quintero, Javier Martinez-Sabando, Miguel Marcilla, Cintia Roodveldt, Jose M. Valpuesta
SCIENTIFIC REPORTS
(2017)
Article
Immunology
Erika Margaret Scholz, Miguel Marcilla, Xavier Daura, David Arribas-Layton, Eddie A. James, Inaki Alvarez
FRONTIERS IN IMMUNOLOGY
(2017)
Article
Biochemical Research Methods
Luis Felipe Clemente, Maria Luisa Hernaez, Antonio Ramos-Fernandez, Gertrudis Ligero, Concha Gil, Fernando Jose Corrales, Miguel Marcilla
JOURNAL OF PROTEOME RESEARCH
(2018)
Article
Biochemical Research Methods
Jennie R. Lill, Peter A. van Veelen, Stefan Tenzer, Arie Admon, Etienne Caron, Joshua E. Elias, Albert J. R. Heck, Miguel Marcilla, Fabio Marino, Markus Mueller, Bjoern Peters, Anthony Purcell, Alessandro Sette, Theo Sturm, Nicola Ternette, Juan Antonio Vizcaino, Michal Bassani-Sternberg
Article
Biochemistry & Molecular Biology
Elena Lorente, Miguel Marcilla, Patricia G. de la Sota, Adriana Quijada-Freire, Carmen Mir, Daniel Lopez
Summary: The identification of HLA class I ligands from virus-infected cells was enhanced by modifying the standard immunopeptidomics strategy to include acid stripping treatment. High throughput mass spectrometry analyses revealed 37 and 51 natural peptides processed and presented in untreated and acid stripping-treated VACV-infected human cells, respectively. Theoretical binding affinity prediction and T cell response characterization in the HLA transgenic mice model showed no differences between HLA ligands identified under the two conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Maria Teresa Bueno-Carrasco, Jorge Cuellar, Marte I. Flydal, Cesar Santiago, Trond-Andre Krakenes, Rune Kleppe, Jose R. Lopez-Blanco, Miguel Marcilla, Knut Teigen, Sara Alvira, Pablo Chacon, Aurora Martinez, Jose M. Valpuesta
Summary: Tyrosine hydroxylase (TH) is a key enzyme in the synthesis of dopamine (DA) and other catecholamines. This study used Cryo-EM to determine the structures of human TH with and without DA, as well as S40 phosphorylated TH. The study revealed the inhibitory and stabilizing effects of DA, as well as the counteraction by S40 phosphorylation, providing insights into the regulatory mechanisms of TH and dopamine homeostasis.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Lucia Quintana-Gallardo, Jaime Martin-Benito, Miguel Marcilla, Guadalupe Espadas, Eduard Sabido, Jose Maria Valpuesta
SCIENTIFIC REPORTS
(2019)