Prevalence of ESBL-producing Enterobacteriaceae in raw vegetables

To determine whether extended-spectrum beta-lactamase ESBL)-producing Enterobacteriaceae ESBL-E) are present in retail raw vegetables in Amsterdam, the Netherlands, we collected 119 samples of 15 different types of vegetables from various sources. After culture, strain identification and susceptibility testing, ESBL-encoding genes were characterised by a microarray. Four of the 15 vegetable types were contaminated with ESBL-E. Seven samples 6 %) yielded ESBL-E. Three bla(CTX-M-15), one bla(CTX-M-1), two genes of the CTX-M-9 group and one SHV ESBL-encoding gene were found. The ESBL genes were similar to what is found in enterobacterial strains from human origin. Therefore, raw vegetables might be a source of resistance genes for the enterobacterial strains found in humans.