Article
Biochemical Research Methods
Cindy Dierikx, Stefan Borjesson, Agnes Perrin-Guyomard, Marisa Haenni, Madelaine Norstrom, Hege H. Divon, Hanna Karin Ilag, Sophie A. Granier, Annette Hammerum, Jette Sejer Kjeldgaard, Natalie Pauly, Luke Randall, Muna F. Anjum, Aleksandra Smialowska, Alessia Franco, Kees Veldman, Jannice Schau Slettemeas
Summary: The study evaluated the performance of different commercially available selective agars for the detection of carbapenemase producing Enterobacteriaceae (CPE). Most agars performed well, but it is important to consider different types of CPE strains when choosing agar type.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Infectious Diseases
Gen Li, Zhihan Ye, Wenyan Zhang, Nianzhen Chen, Yangqin Ye, Yuchao Wang, Fei Wu, Keli Wang, Lieying Fan
Summary: In this study, the researchers developed a rapid method for identifying carbapenemase types using liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) and demonstrated its potential reliability in future clinical applications.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Eva Maria Parisio, Giulio Camarlinghi, Marco Coppi, Claudia Niccolai, Alberto Antonelli, Maria Nardone, Chiara Vettori, Tommaso Giani, Romano Mattei, Gian Maria Rossolini
Summary: This study compared fosfomycin susceptibility testing using different methods for multidrug-resistant Enterobacterales and Pseudomonas aeruginosa, with the commercial AD Fosfomycin showing good concordance with the reference AD method.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Immunology
Pan Fu, Xinpeng Luo, Jun Shen, Leiyan He, Hua Rong, Chunling Li, Saige Chen, Lei Zhang, Aimin Wang, Chuanqing Wang
Summary: This study investigated carbapenemase-producing Enterobacteriaceae (CPE) strains isolated from children in Shanghai, China from 2016 to 2021. The results showed that NDM was the primary carbapenemase in children (67.6%), followed by KPC (26.4%), IMP (5.3%), and OXA-48 (0.6%). NDM-Kpn posed the most serious threat to infants, especially neonates, and the high-risk clone ST11 KPC-Kpn should be continuously monitored in children.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Laura Berneking, Anna Both, Benjamin Berinson, Armin Hoffmann, Marc Luetgehetmann, Martin Aepfelbacher, Holger Rohde
Summary: The BD Phoenix CPO detect (PCD) assay showed high sensitivity in detecting and classifying carbapenemase-producing strains in Enterobacterales and non-fermentative gram-negative rods. However, due to low specificity and potential erroneous classification, especially in non-fermentative gram-negative rods, confirmation by alternative methods is required.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Yong Kyun Kim, In Bok Chang, Han Sung Kim, Wonkeun Song, Seung Soon Lee
Summary: The study found that OXA-48-like and Klebsiella pneumoniae are associated with prolonged carriage of CPE. Additionally, patients with CP-K. pneumoniae, positive clinical specimen of CPE, and concurrent Clostridioides difficile infection are more likely to have prolonged carriage of CPE.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Infectious Diseases
Sarah Jolivet, Jeanne Couturier, Xavier Vuillemin, Cyril Gouot, Didier Nesa, Marine Adam, Eolia Brissot, Mohamad Mohty, Remy A. Bonnin, Laurent Dortet, Frederic Barbut
Summary: This study investigated a persistent outbreak of OXA-48 carbapenemase-producing Enterobacterales (CPE) in a haematological ward of a French teaching hospital, primarily Citrobacter freundii. Whole genome comparisons identified clonal dissemination from hospital environment to patients. Strict routine infection control measures and an intensive cleaning program helped to contain the outbreak.
Article
Infectious Diseases
Michael R. Hovan, Navaneeth Narayanan, Vanessa Cedarbaum, Tanaya Bhowmick, Thomas J. Kirn
Summary: This study compared the mortality rates between patients with carbapenemase-producing CRE and non-carbapenemase-producing CRE, showing that non-carbapenemase-producing CRE patients had a higher risk of death at 30 days after onset compared to carbapenemase-producing CRE patients.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Infectious Diseases
Seung Hye Lee, Chan Hee Kim, Hee Young Lee, Kun Hee Park, Su Ha Han
Summary: This study examines the trends and epidemiological characteristics of carbapenem-resistant Enterobacteriaceae (CRE) occurrence in Gyeonggi Province over the past four years. The study finds that CRE infections are endemic in Gyeonggi Province, particularly in areas with a high number of healthcare facilities. Older adults constitute the majority of patients with CRE bacteremia, and antibiotic use does not significantly affect mortality risk.
Article
Immunology
Tom J. B. de Man, Anna Q. Yaffee, Wenming Zhu, Dhwani Batra, Efe Alyanak, Lori A. Rowe, Gillian McAllister, Heather Moulton-Meissner, Sandra Boyd, Andrea Flinchum, Rachel B. Slayton, Steven Hancock, Maroya Spalding Walters, Alison Laufer Halpin, James Kamile Rasheed, Judith Noble-Wang, Alexander J. Kallen, Brandi M. Limbago
Summary: This investigation identified the largest known outbreak of VIM-producing CRE in the United States, showcasing evidence of in-hospital plasmid transmission and environmental contamination.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Engineering, Multidisciplinary
Beiwen Zheng, Hao Xu, Lihua Guo, Xiao Yu, Jinru Ji, Chaoqun Ying, Yunbo Chen, Ping Shen, Huiming Han, Chen Huang, Shuntian Zhang, Tao Lv, Yonghong Xiao
Summary: This study investigates the population structure and genetic characteristics of carbapenemase-producing Enterobacteriaceae (CPE) isolates among bloodstream infections (BSIs) in China. The research reveals a high prevalence of ESBLs and wide dissemination of CPE in BSIs, with the clonal spread of KPC2-producing K. pneumoniae ST11 and NDM-5-producing E. coli ST167 being of particular concern.
Article
Infectious Diseases
Ryuichiro Abe, Yukihiro Akeda, Tetsuya Iida, Shigeyuki Hamada
Summary: The article discusses the validity of population analysis profiling (PAP) for determining carbapenem heteroresistance. The authors found that some isolates did not exhibit heteroresistance despite meeting PAP criteria. It was discovered that bacterial cells that were originally non-viable could occasionally become viable due to the hydrolysis of carbapenems by carbapenemases produced by dying cells, mimicking the emergence of subpopulations with enhanced resistance. As a result, PAP for carbapenemase-producing Enterobacteriaceae (CPE) may be influenced by dying populations and may not accurately detect heterogeneity in carbapenem resistance among seemingly isogenic clones.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Diego Cuicapuza, Luis Alvarado, Norah Tocasca, Daniel Aguilar, Juan Carlos Gomez-de-la-Torre, Guillermo Salvatierra, Pablo Tsukayama, Jesus Tamariz
Summary: We identified five carbapenemase-producing Enterobacterales (CPE) isolates from two healthcare institutions in Lima, Peru. All isolates were carriers of the bla(OXA-48)-like gene and had the bla(OXA-181) gene as the only carbapenemase gene. Other resistance genes and the IncX3 plasmid were also found. This study highlights the increasing public health problem of CPE isolates harboring bla(OXA)-like genes and the wide dissemination of bla(OXA-181) in Peru.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Assiya El Kettani, Fakhreddine Maaloum, Nehemie Nzoyikorera, Mohamed Khalis, Khalid Katfy, Houria Belabbes, Khalid Zerouali
Summary: The study compared the new rapid immunochromatographic test with PCR and EUCAST algorithm for detecting carbapenemase-producing Enterobacterales (CPE) and found that the rapid IC test showed 100% sensitivity and specificity for detecting certain carbapenemases. This suggests that the rapid IC test could be an effective tool for rapid diagnosis and treatment of CPE infections.
Article
Infectious Diseases
Chendi Zhu, Carmen Li, Christopher K. C. Lai, Rita Ng, Ka Yee Chau, Kam Tak Wong, Norman W. S. Lo, Nilakshi Barua, Ying Yang, Veranja Liyanapathirana, Mamie Hui, Raymond W. Lai, Kitty S. C. Fung, Dominic N. Tsang, I. P. Margaret
Summary: This study describes the molecular epidemiology of carbapenemase-producing Enterobacteriaceae (CPE) in patients from Hong Kong hospitals, with increasing isolation rates and dominance of bla(NDM) and bla(IMP) genes. Enhanced containment strategies are required to mitigate the rising trend of CPE in healthcare settings.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
K. van der Zwaluw, S. Witteveen, L. Wielders, M. van Santen, F. Landman, A. de Haan, L. M. Schouls, T. Bosch
CLINICAL MICROBIOLOGY AND INFECTION
(2020)
Letter
Infectious Diseases
Elske Sieswerda, Marre van den Brand, Roland B. van den Berg, Joris Strater, Leo Schouls, Karin van Dijk, Andries E. Budding
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Infectious Diseases
J. W. Timoteus Deelen, W. C. Rottier, A. G. M. Buiting, J. W. Dorigo-Zetsma, J. A. J. W. Kluytmans, P. D. Van der Linden, S. F. T. Thijsen, B. J. M. Vlaminckx, A. J. L. Weersink, H. S. M. Ammerlaan, M. J. M. Bonten, C. H. Van Werkhoven
Summary: The study found that short-course adjunctive aminoglycoside treatment along with beta-lactam antibiotics did not improve 30-day mortality in sepsis patients with Gram-negative bloodstream infection, despite providing better antibiotic coverage of pathogens.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Infectious Diseases
Wouter C. Rottier, J. W. Timoteus Deelen, Giorgia Caruana, Anton G. M. Buiting, J. Wendelien Dorigo-Zetsma, Jan A. J. W. Kluytmans, Paul D. van der Linden, Steven F. T. Thijsen, Bart J. M. Vlaminckx, Annemarie J. L. Weersink, Heidi S. M. Ammerlaan, Marc J. M. Bonten
Summary: The study found that antibiotic resistance in Gram-negative bacteria did not increase 30-day mortality in infections in the Netherlands. Among the 1954 Gram-negative infections studied, only 12% were resistant, and the impact of antibiotic resistance on mortality was relatively minor.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Infectious Diseases
Audrey J. C. Overgaauw, David C. de Leeuw, Susanne P. Stoof, Karin van Dijk, Joost C. J. Bot, Eef J. Hendriks
BMC INFECTIOUS DISEASES
(2020)
Article
Infectious Diseases
J. W. Timoteus Deelen, Wouter C. Rottier, Cornelis H. van Werkhoven, Sjoukje H. S. Woudt, Anton G. M. Buiting, J. Wendelien Dorigo-Zetsma, Jan A. J. W. Kluytmans, Paul D. van der Linden, Steven F. T. Thijsen, Bart J. M. Vlaminckx, Annemarie J. L. Weersink, Heidi S. M. Ammerlaan, Marc J. M. Bonten
JOURNAL OF INFECTION
(2020)
Article
Multidisciplinary Sciences
Antoni P. A. Hendrickx, Fabian Landman, Angela de Haan, Dyogo Borst, Sandra Witteveen, Marga G. van Santen-Verheuvel, Han G. J. van der Heide, Leo M. Schouls
SCIENTIFIC REPORTS
(2020)
Article
Microbiology
Frank van Someren Greve, Romy du Long, Raju Talwar, Charlotte J. P. Beurskens, Huibertus J. Voerman, Karin van Dijk
Summary: Diagnosing COVID-19-associated pulmonary aspergillosis can be challenging due to the invasiveness of diagnostic procedures required. This article presents one of the first proven cases of COVID-19-associated pulmonary aspergillosis through positive post-mortem lung biopsy culture.
Article
Microbiology
Elske Sieswerda, Thijs Bosch, Jacqueline M. Lankelma, Leo M. Schouls, Karin van Dijk
Summary: The study aimed to define the sensitivity and specificity of Vitek 2 MIC values as a phenotypic screening method for carbapenemase-producing Pseudomonas aeruginosa. The results indicated that Vitek 2 MIC values above specific cutoff points could effectively screen for carbapenemase-producing P. aeruginosa.
FUTURE MICROBIOLOGY
(2020)
Article
Public, Environmental & Occupational Health
Wouter C. Rottier, Mette Pinholt, Akke K. van der Bij, Magnus Arpi, Sybrandus N. Blank, Marrigje H. Nabuurs-Franssen, Gijs J. H. M. Ruijs, Matthijs Tersmette, Jacobus M. Ossewaarde, Rolf H. Groenwold, Henrik Westh, Marc J. M. Bonten
Summary: The study revealed that compared to ampicillin-resistant Enterococcus faecium (ARE) bacteremia, vancomycin-resistant E. faecium (VRE) bacteremia was associated with higher 30-day mortality. The increase in mortality risk with VRE was not mediated by a delay in appropriate antibiotic therapy.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2022)
Review
Infectious Diseases
Roel P. J. Willems, Karin van Dijk, Maria J. G. T. Vehreschild, Lena M. Biehl, Johannes C. F. Ket, Sharon Remmelzwaal, Christina M. J. E. Vandenbroucke-Grauls
Summary: This study aims to quantify the effect of gut colonisation on the subsequent risk of infection with multidrug-resistant bacteria. The results showed a substantial risk of infection, particularly in patients colonised with carbapenem-resistant Gram-negative bacteria. These data can guide prophylactic and treatment decisions and serve as a valuable resource for planning clinical trials on targeted prevention.
LANCET INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Antoni P. A. Hendrickx, Rogier P. Schade, Fabian Landman, Thijs Bosch, Leo M. Schouls, Karin van Dijk
Summary: The study conducted a detailed analysis of three carbapenemase-producing A. ursingii strains, identifying similar plasmids carrying the blaIMP-4 and bla OXA-58 genes, suggesting a potential origin from A. johnsonii.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Medicine, Research & Experimental
Willem J. J. Falkenburg, Marit Jalink, Marie Jose Kersten, Jochem B. Buil, Karin van Dijk
Summary: Fungal infections after CAR-T cell therapy are rare but can occur. We report a case of Scedosporium apiospermum infection causing bursitis of the elbow in a lymphoma patient after CAR-T cell treatment. The infection relapsed under posaconazole treatment, but was cured after surgical extirpation of the bursa.
MEDICAL MYCOLOGY CASE REPORTS
(2022)
Article
Microbiology
Jochem B. Buil, Sammy Huygens, Albert Dunbar, Alexander Schauwvlieghe, Marijke Reynders, Diana Langerak, Karin van Dijk, Anke Bruns, Pieter-Jan Haas, Douwe F. Postma, Bart Biemond, Fatima Zohra Delma, Elizabeth de Kort, Willem J. G. Melchers, Paul E. Verweij, Bart Rijnders, Dutch Belgian Mycosis Study Grp
Summary: In this study, the performance of an automated chemiluminescence immunoassay (CLIA) with bronchoalveolar lavage (BAL) fluid samples was evaluated. It was found that CLIA performed equally well as the traditional enzyme-linked immunosorbent assays (ELISAs) in diagnosing invasive pulmonary aspergillosis (IPA), with the added benefit of a faster turnaround time.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Immunology
Pieter P. A. Lestrade, Jochem B. Buil, Martha T. van der Beek, Ed J. Kuijper, Karin van Dijk, Greetje A. Kampinga, Bart J. A. Rijnders, Alieke G. Vonk, Sabine C. de Greeff, Annelot F. Schoffelen, Jaap van Dissel, Jacques F. Meis, Willem J. G. Melchers, Paul E. Verweij
EMERGING INFECTIOUS DISEASES
(2020)