Article
Microbiology
Julian Sommer, Kristina M. Gerbracht, Felix F. Krause, Florian Wild, Manuela Tietgen, Sara Riedel-Christ, Janko Sattler, Axel Hamprecht, Volkhard A. J. Kempf, Stephan Goettig
Summary: The study identified OXA-484 as a carbapenemase with high sequence similarity plasmids, showing low frequency in horizontal gene transfer. OXA-484 exhibits antibiotic activity between OXA-232 and OXA-244, demonstrating unique resistance characteristics.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Alexander Wunderlich, Kyriaki Xanthopoulou, Julia Wille, Esther Wohlfarth, Stefanie Gerson, Martin Kaase, Harald Seifert, Paul G. Higgins
Summary: This study aimed to characterize the genetic environment of metallo-beta-lactamases (MBL) in carbapenem-resistant clinical Acinetobacter pittii isolates. The results showed the existence of a promiscuous plasmid circulating in A. pittii isolates in Germany with high similarity in the plasmid backbone but differences in the MBL-encoding region.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Neda Yousefi Nojookambari, Mehrzad Sadredinamin, Razieh Dehbanipour, Zohreh Ghalavand, Gita Eslami, Maryam Vaezjalali, Bahram Nikmanesh, Sajjad Yazdansetad
Summary: The study examined the antibiotic resistance, prevalence of beta-lactamase-encoding genes, and clonal relationships of Acinetobacter baumannii strains isolated from pediatric patients in Tehran, Iran. The results showed high rates of multi-drug resistant and extensively drug-resistant isolates, with certain beta-lactamase genes being prevalent. The presence of well-established clones poses a potential therapeutic challenge due to genetic elements associated with resistance.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Infectious Diseases
Nouf Al-Rashed, Khalid M. Bindayna, Mohammad Shahid, Nermin Kamal Saeed, Abdullah Darwish, Ronni Mol Joji, Ali Al-Mahmeed
Summary: This study collected 50 carbapenem-resistant Acinetobacter baumannii (CRAB) isolates from four main hospitals in Bahrain. The results showed that all isolates were resistant to imipenem, meropenem, and cephalosporins, and harbored various resistance genes. The study emphasizes the importance of strengthening surveillance and stringent infection control measures in clinical settings to prevent the further spread of such isolates.
Article
Environmental Sciences
Jakub Hubeny, Ewa Korzeniewska, Martyna Buta-Hubeny, Wiktor Zielinski, Damian Rolbiecki, Monika Harnisz
Summary: This study aimed to analyze the prevalence of carbapenem resistance genes in Acinetobacter spp. isolated from wastewater in a municipal WWTP and to determine their spread from treated wastewater to river water. The research found evidence that WWTP is a potential source of pollution, leading to the spread of alarming beta-lactam resistance genes in the natural environment, posing a serious epidemiological threat.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Agriculture, Dairy & Animal Science
Zui Wang, Qin Lu, Xiaohui Mao, Li Li, Junfeng Dou, Qigai He, Huabin Shao, Qingping Luo
Summary: ESBL-producing E.coli strains isolated from chickens in central China showed high resistance to cephalosporins and harbored diverse ESBL-encoding genes. These strains pose a significant public health risk.
Review
Pharmacology & Pharmacy
Amer El-Ghali, Ashlan Kunz J. Coyne, Kaylee Caniff, Callan Bleick, Michael J. Rybak
Summary: SUL-DUR is a novel beta-lactam-beta-lactamase inhibitor designed for the treatment of carbapenem-resistant Acinetobacter baumannii (CRAB) infections. The FDA's fast-track approval for SUL-DUR is pending after the completion of the phase III ATTACK trial, which showed that SUL-DUR was non-inferior to colistin with a better safety profile. With limited treatment options available, SUL-DUR holds promise for the treatment of CRAB infections.
Article
Immunology
Jose Cedano, Michelle Baez, Fernando Pasteran, Sabrina Daiana Montana, Grace Ra, Venjaminne Fua, Alejandra Corso, Marcelo E. Tolmasky, Robert A. Bonomo, Maria Soledad Ramirez
Summary: This study tested the efficacy of the combination of sulbactam and a novel DBO, zidebactam, against carbapenem-resistant A. baumannii isolates. The results showed that the sulbactam/zidebactam combination was able to restore sulbactam susceptibility in resistant isolates, including those resistant to the sulbactam/avibactam combination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Pei Li, Zhishuang Yang, Ting Lei, Yujie Dai, Yang Zhou, Dekang Zhu, Hongyan Luo
Summary: This study elucidated the role of a novel carbapenem-hydrolysing class D beta-lactamase RAD-1 from Riemerella anatipestifer. The RAD-1 enzyme showed distinct characteristics from other class D beta-lactamase and was widely prevalent and conserved in R. anatipestifer. Expression of RAD-1 in E. coli resulted in increased resistance to various beta-lactam antibiotics.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Satomi Takei, Yu Jie Lu, Mari Tohya, Shin Watanabe, Shigeki Misawa, Yoko Tabe, Takashi Miida, San Mya, Htay Htay Tin, Tatsuya Tada, Teruo Kirikae
Summary: This study demonstrates the spread of carbapenem-resistant K. pneumoniae strains in medical settings in Myanmar. Specifically, plasmid genes encoding NDMs and 16S rRNA methylases have been spreading in high-risk clones of K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Samy Selim, Osama Ahmed Faried, Mohamed S. Almuhayawi, Osama A. Mohammed, Fayez M. Saleh, Mona Warrad
Summary: Acinetobacter baumannii is a nonfermenting Gram-negative bacterium associated with nosocomial infections. This study identified four distinct lineages of A. baumannii and found that genotype (A) isolates showed reduced effectiveness to certain antibiotics. Additionally, the binding affinity of imipenem towards Acinetobacter baumannii OXA beta-lactamase enzymes was investigated.
Article
Infectious Diseases
Pai-Wei Su, Emirlyn Cheng Yang, Sin-Hua Moi, Cheng-Hong Yang, Li-Yeh Chuang
Summary: The problem of antibiotic-resistant strains is a global public health issue. This study focused on the multi-drug resistant Acinetobacter baumannii and identified several drug resistance genes in these strains. The study also found that these genes influenced biofilm formation and antibiotic resistance patterns.
Article
Infectious Diseases
Dewi Anggraini, Dewi Santosaningsih, Yulia Rosa Saharman, Pepy Dwi Endraswari, Cahyarini Cahyarini, Leli Saptawati, Zinatul Hayati, Helmia Farida, Cherry Siregar, Munawaroh Pasaribu, Heriyannis Homenta, Enty Tjoa, Novira Jasmin, Rosantia Sarassari, Wahyu Setyarini, Usman Hadi, Kuntaman Kuntaman
Summary: Carbapenem non-susceptible Acinetobacter baumannii (CNSAB) is a significant pathogen causing nosocomial bacteremia among critically ill patients worldwide. This study analyzed the genetic profiles of CNSAB isolates from bacteremia patients in Indonesia and found that they were mostly resistant to multiple antibiotic classes, but susceptible to tigecycline and trimethoprim-sulfamethoxazole. The bla(OXA-23-like) gene was identified as the predominant gene. A national surveillance system is needed to monitor the genetic profiles of CNSAB in Indonesia.
Article
Chemistry, Multidisciplinary
Catherine L. Tooke, Philip Hinchliffe, Michael Beer, Kirill Zinovjev, Charlotte K. Colenso, Christopher J. Schofield, Adrian J. Mulholland, James Spencer
Summary: KPC-2 is a carbapenemase that confers resistance to β-lactam antibiotics in Gram-negative pathogens. The flexible a-loop in KPC-2 plays a role in catalytic activity, while carbapenemase activity is mainly due to efficient deacylation of the A2-enamine acyl-enzyme tautomer. Quantum mechanics/molecular mechanics simulations reveal the specific reactivity of the two isomers.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Microbiology
Laura Dabos, Joanna E. Raczynska, Pierre Bogaerts, Agustin Zavala, Delphine Girlich, Remy A. Bonnin, Laurent Dortet, Aurelie Peyrat, Pascal Retailleau, Bogdan I. Iorga, Mariusz Jaskolski, Youri Glupczynski, Thierry Naas
Summary: OXA-48-producing Enterobacterales have disseminated globally, with several variants reported, differing by a few amino acid substitutions or deletions, mostly in the /35-/36 loop region. OXA-517, a natural variant of OXA-48, with an Arg214Lys substitution and a deletion in the β5-β6 loop, has the ability to hydrolyze extended-spectrum cephalosporins and carbapenems. This study highlights the remarkable ability of OXA-48-like carbapenemases to evolve through mutation/deletion in the β5-β6 loop to expand their hydrolysis spectrum.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Yan Chen, Lu Sun, Xiaoliang Ba, Shengnan Jiang, Hemu Zhuang, Feiteng Zhu, Haiping Wang, Peng Lan, Qiucheng Shi, Zhengan Wang, Yiyi Chen, Keren Shi, Shujuan Ji, Yan Jiang, Mark A. Holmes, Yunsong Yu
Summary: This study investigated the genomic epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in China and found a high prevalence of the CC59 lineage in community-associated MRSA. Almost all isolates in the CC59 lineage showed genetic variants leading to increased beta-lactam susceptibility, suggesting potential for improved antibiotic stewardship.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Feiteng Zhu, Hemu Zhuang, Lingfang Di, Zhengan Wang, Yiyi Chen, Shengnan Jiang, Chao Gu, Lu Sun, Haiping Wang, Yiwei Zhu, Peng Lan, Dandan Wu, Yunsong Yu, Shujuan Ji, Yan Chen
Summary: This study evaluated the susceptibility of 472 methicillin-resistant Staphylococcus aureus (MRSA) isolates to ceftobiprole (BPR) and investigated the mechanisms underlying BPR resistance. The results showed that community-associated MRSA isolates were more susceptible to BPR than healthcare-associated MRSA isolates. Nanopore sequencing revealed SCCmec repeat-mediated resistance mechanism in BPR-resistant MRSA isolates, and mutations in multiple genes were found to potentially contribute to BPR-resistance development in MRSA.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Letter
Immunology
Dandan Wu, Yiyi Chen, Lu Sun, Yunsong Yu, Yan Chen
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Haiping Wang, Dandan Wu, Lingfang Di, Feiteng Zhu, Zhengan Wang, Lu Sun, Yiyi Chen, Shengnan Jiang, Hemu Zhuang, Mengzhen Chen, Shujuan Ji, Yan Chen
Summary: This study determined the genetic structure of ermB-positive Tn1546-like mobile elements in methicillin-resistant Staphylococcus aureus (MRSA) from mainland China. The study found that these elements were widely disseminated in CC5-ST965 MRSA. Molecular epidemiological study of Tn1546-like elements is crucial for preventing the spread of resistance genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Yiyi Chen, Shujuan Ji, Lu Sun, Haiping Wang, Feiteng Zhu, Mengzhen Chen, Hemu Zhuang, Zhengan Wang, Shengnan Jiang, Yunsong Yu, Yan Chen
Summary: This study identified a novel gene, fosY, which confers fosfomycin resistance in Staphylococcus aureus. The gene is located in a genomic island, RI (fosY), and can promote cross-species gene transfer and confer resistance to CC1 MRSA. This highlights the importance of genetic surveillance of resistance genes among MRSA isolates.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Infectious Diseases
Danni Bao, Linyao Huang, Jianxin Yan, Yexuzi Li, Zhi Ruan, Tian Jiang
Summary: This study described the genomic characteristics of a P. putida isolate co-carrying five beta-lactam resistance genes recovered from a urinary tract infection in China. The isolate showed resistance to a wide range of antimicrobial agents and could be assigned to a new sequence type. The presence of multiple beta-lactamase genes suggests that this isolate has the potential to be an antibiotic resistance reservoir for Pseudomonas spp.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Linna Xu, Fen Wan, Hao Fu, Biao Tang, Zhi Ruan, Yonghong Xiao, Qixia Luo
Summary: In our study, we identified mcr-10-carrying isolates, including multidrug-resistant ST11 Klebsiella pneumoniae isolates coharboring mcr-10 and mcr-8 genes, which are resistant to polymyxins and show reduced susceptibility to tigecycline. Through screening 941,449 bacterial genomes in the GenBank database, we found a sporadic distribution of mcr-10-harboring isolates worldwide in various sources, especially in humans, highlighting the need for close monitoring and concern in clinical settings.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yingying Kong, Chao Li, Gangfeng Li, Ting Yang, Mohamed S. Draz, Xinyou Xie, Jun Zhang, Zhi Ruan
Summary: Fluoroquinolone resistance in Mycoplasma hominis and Ureaplasma spp. is increasing globally. This study evaluated the antimicrobial activity of two new fluoroquinolones, delafloxacin and finafloxacin, for the first time, against clinical isolates of M. hominis and Ureaplasma spp. The results showed that delafloxacin had lower minimum inhibitory concentrations against fluoroquinolone-resistant isolates of both M. hominis and Ureaplasma spp.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Zhengan Wang, Chao Gu, Lu Sun, Feng Zhao, Ying Fu, Lingfang Di, Junxiong Zhang, Hemu Zhuang, Shengnan Jiang, Haiping Wang, Feiteng Zhu, Yiyi Chen, Mengzhen Chen, Xia Ling, Yan Chen, Yunsong Yu
Summary: Staphylococcus capitis is a common cause of bloodstream infections in neonatal intensive care units, with multidrug-resistant isolates complicating treatment. The aim of this study was to establish a core genome multilocus sequence typing (cgMLST) scheme to document the transmission and dissemination of multidrug-resistant S. capitis isolates.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Chemistry, Medicinal
Zhi Ruan, Mohamed A. A. Dkhil
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Article
Infectious Diseases
Min Yang, Gufeng Xu, Zhi Ruan, Yue Wang
Summary: This study reports the genomic characteristics of a multidrug-resistant Escherichia coli strain harboring blaNDM-5 and blaCTX-M-14 genes detected in a pediatric patient. These data would help us better understand the prevalence, genetic characteristics, and mechanisms of antimicrobial resistance of this recently identified multidrug-resistant bacteria in children.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Yuan Ge, Gufeng Xu, Zhi Ruan, Yue Wang
Summary: This study investigated the genomic and phylogenetic characteristics of a L. monocytogenes ST1 isolate obtained from the blood sample of a woman with a stillbirth in the third trimester. The antimicrobial resistance genes, serotype, and genetic relatedness with other isolates were analyzed. The findings contribute to understanding the genomic features, antimicrobial resistance mechanisms, and epidemiological characteristics of this pathogen.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Linli Xing, Yiyi Chen, Xia Ling, Dandan Wu, Lu Sun, Jingrong Lin, Yan Chen
Summary: This study reports the analysis of MRSA strains from a patient with livestock association. Whole-genome sequencing and comparative genomic analyses revealed the correlation of these strains with the common ST7 lineage in retail meat and meat products in China. Additionally, presence of plasmid and prophage islands were identified. ST7 may originate from livestock and be transmitted in communities.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Pharmacology & Pharmacy
Yuye Wu, Tian Jiang, Danni Bao, Meina Yue, Huiqiong Jia, Jianyong Wu, Zhi Ruan
Summary: Due to the frequent international and intercontinental transmission of multidrug-resistant bacteria, it is imperative to understand the epidemiology, phylogeography, and population structure of carbapenem-resistant Salmonella enterica (CRSE) across the globe. Our findings on the worldwide spread and transmission dynamics of this emerging bacterium has increased the knowledge of its global epidemics. Continued epidemiological surveillance is necessary to prevent global outbreak of multidrug-resistant Salmonella infections.
DRUG RESISTANCE UPDATES
(2023)
Article
Infectious Diseases
Yiyi Chen, Lu Sun, Yueqin Hong, Mengzhen Chen, Hao Zhang, Yaqin Peng, Kang Liao, Haiping Wang, Feiteng Zhu, Hemu Zhuang, Zhengan Wang, Shengnan Jiang, Yunsong Yu, Yan Chen
Summary: The overuse of antibiotics in livestock is contributing to the burden of antimicrobial resistance in humans, and the emergence of LA-MRSA is a growing concern. This study identified the resistance mechanisms and genetic features of clinical ST9 MRSA isolates and found that they were multidrug-resistant and resistant to third-generation tetracyclines. Phylogenetic analysis revealed a potential transmission of ST9 isolates between humans and livestock. The study highlights the importance of adopting a One Health approach to control antibiotic resistance.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
