期刊
EUROPEAN JOURNAL OF CELL BIOLOGY
卷 87, 期 2, 页码 101-110出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.ejcb.2007.09.001
关键词
vascular remodeling; vascular smooth muscle cell; spontaneously hypertensive rat; RhoGDI alpha; proteomics
类别
Arteries undergo remodeling as a consequence of increased wall stress during hypertension. However, the molecular mechanisms of the vascular remodeling are largely unknown. Proteomics is a powerful tool to screen for differentially expressed proteins, but little effort was made on vascular disease research, especially on hypertension. In the present study, the differentially expressed proteins in aortas from 18-week-old spontaneously hypertensive rats (SHR) and their normotensive counterpart, Wistar Kyoto rats (WKY), were examined by two-dimensional electrophoresis (2-DE). We found 50 proteins to be differentially expressed, among which 27 were highly or only expressed in SHR and 23 in WKY. Using matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOFMS) and online data search, nine proteins, including Rho GDP dissociation inhibitor alpha (RhoGDI alpha), were identified with high confidence. Further, the upregulation of RhoGDI alpha was verified at both mRNA and protein level in SHR. In addition, when cultured vascular smooth muscle cells (VSMCs) from aortas of SHR and WKY were treated with angiotensin II (Ang II) and antagonist of angiotensin II type I (AT(1)) receptor, L158809, respectively, RhoGDIa was upregulated by Ang II and downregulated by L158809 in VSMCs of SHR. These results demonstrate that vascular remodeling results in significant alterations in the protein expression profile of the aorta during hypertension and suggest that the upregulation of RhoGDI alpha in hypertension is induced by Ang II via AT(1) receptor. (c) 2007 Elsevier GmbH. All rights reserved.
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