Article
Cardiac & Cardiovascular Systems
Xi Lou, Yawen Tang, Lei Ye, Danielle Pretorius, Vladimir G. Fast, Asher M. Kahn-Krell, Jue Zhang, Jianhua Zhang, Aijun Qiao, Gangjian Qin, Timothy Kamp, James A. Thomson, Jianyi Zhang
Summary: The study demonstrates that human cardiac muscle patches containing three types of cardiac cells derived from human pluripotent stem cells significantly improved cardiac function, reduced infarct size, and promoted cardiac maturation in a pig model of myocardial infarction. The inclusion of cardiac fibroblasts in the cardiac patches enhanced the maturity and function of cardiac muscle cells. Transplantation of cardiac patches with cardiac fibroblasts resulted in better cardiac recovery and engraftment compared to patches without fibroblasts.
CARDIOVASCULAR RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Maryam Torabi, Mohammad Foad Abazari, Shohreh Zare Karizi, Mina Kohandani, Nazanin Hajati-Birgani, Sara Norouzi, Fatemeh Nejati, Alireza Mohajerani, Tahereh Rahmati, Zakiye Mokhames
Summary: In this study, PLGA and PRP were used to fabricate nanofibrous scaffolds, and it was found that iPSCs cultured on PLGA-PRP nanofibers were more likely to differentiate into cardiomyocytes compared to PLGA. Multidimensional evaluations at the morphological, molecular gene, and protein expression levels indicated the potential of PLGA-PRP in cardiac tissue engineering.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2021)
Article
Engineering, Biomedical
Shuai Deng, Xiaoyu Zhao, Yanlun Zhu, Ning Tang, Rongliang Wang, Xuerao Zhang, Fuyang Qu, Yi-Ping Ho, Wayne Yuk-Wai Lee, Jiansu Chen, Mingqiang Li, Yu Tao, Hon Fai Chan
Summary: Liver tissue engineering is advanced through the fabrication of collagen type I microspheres, enabling efficient hepatic differentiation of stem cells and assembly of prevascularized liver tissue. The microspheres exhibit high uniformity and can self-assemble with endothelial cells to form high cell-packing density liver tissue. This technology has potential applications in regenerative medicine, drug screening, and in vitro liver modeling.
Review
Biochemistry & Molecular Biology
Shudong Zhao, Jishizhan Chen, Lei Wu, Xin Tao, Naheem Yaqub, Jinke Chang
Summary: Skeletal muscle, responsible for crucial functions in the body, can suffer from severe injuries leading to volumetric muscle loss (VML). Tissue-engineered muscles using induced pluripotent stem cells (iPSCs) offer a potential solution to restore muscle function. iPSCs, with their pluripotency and self-renewal capacity, can be used to construct tissue-engineered artificial skeletal muscles for various applications. These iPSC-based models also have the potential to revolutionize drug discovery and reduce reliance on animal models. This review provides an overview of iPSCs in tissue-engineered skeletal muscles, discussing advancements, challenges, and considerations for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Chemistry, Medicinal
Farah S. Raad, Taukeer A. Khan, Tilman U. Esser, James E. Hudson, Bhakti Irene Seth, Buntaro Fujita, Ravi Gandamala, Lutz F. Tietze, Wolfram H. Zimmermann
Summary: Human pluripotent stem cells have great potential in cardiovascular applications, with bone morphogenetic protein 4 playing a key role in cardiac mesoderm induction. Chalcones were identified as potential inducers of cardiac mesoderm, with 4'-hydroxychalcone showing effectiveness in deriving contractile cardiomyocytes.
Article
Multidisciplinary Sciences
Karlijn A. L. Hasaart, Freek Manders, Joske Ubels, Mark Verheul, Markus J. van Roosmalen, Niels M. Groenen, Rurika Oka, Ewart Kuijk, Susana M. Chuva de Sousa Lopes, Ruben van Boxtel
Summary: This study compares the mutation burden between cultured iPSCs and their isogenic embryonic cells, finding that iPSCs maintain genomic integrity during culture at a similar degree as pluripotent cells in vivo.
Review
Cell Biology
Muhammad Shahid Javaid, Tracie Tan, Naomi Dvir, Alison Anderson, Terence J. O'Brien, Patrick Kwan, Ana Antonic-Baker
Summary: This review reports the current information and progress in using induced pluripotent stem cells to generate patient-specific models of epilepsy, which can be used to study the pathological mechanisms of epilepsy and drug development.
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Multidisciplinary Sciences
Yin-Yu Lam, Chun-Ho Chan, Lin Geng, Nicodemus Wong, Wendy Keung, Yiu-Fai Cheung
Summary: Cardiomyocytes can be derived from hiPSC lines, but the efficacy varies. By performing single cell transcriptomics, researchers identified cardiac and extra-cardiac lineages during differentiation. They found APLNR as a surface marker for in vitro cardiac progenitors, and using this marker, they isolated and differentiated cardiac progenitors from multiple hiPSC lines. This study has potential applications in studying cardiac development, disease modeling, and cardiac regeneration.
Review
Cardiac & Cardiovascular Systems
Crystal C. Kennedy, Erin E. Brown, Nadia O. Abutaleb, George A. Truskey
Summary: The vascular endothelium plays a crucial role in maintaining healthy vessel function throughout the body. Differentiated endothelial cells from induced pluripotent stem cells are widely used in in vitro models and in vivo applications. Furthermore, the use of pluripotent stem cells offers advantages in avoiding difficulties associated with sourcing primary endothelial cells.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Review
Cardiac & Cardiovascular Systems
Mengcheng Shen, Thomas Quertermous, Michael P. Fischbein, Joseph C. Wu
Summary: The developmental origin of vascular smooth muscle cells plays a crucial role in regional susceptibility and resistance to vascular diseases. Patient induced pluripotent stem cell-derived VSMCs are valuable for research and therapeutic applications, but the heterogeneity in developmental origins poses challenges.
CIRCULATION RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Amit Gruber, Oded Edri, Shany Glatstein, Idit Goldfracht, Irit Huber, Gil Arbel, Amira Gepstein, Snizhanna Chorna, Lior Gepstein
Summary: This study aimed to develop high-resolution optogenetic approaches to modulate electrical activity in 2D and 3D cardiac tissue models derived from human induced pluripotent stem cell cardiomyocytes. The results demonstrated the ability to control the conduction and contractile properties of cardiac cells using optogenetics, and to use light-controllable techniques for cardiac resynchronization therapy and arrhythmia termination. This approach has important applications in cardiac physiology, pathophysiology, and arrhythmia treatment.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Sabine Julia Maria Sag, Karin Menhart, Jirka Grosse, Florian Hitzenbichler, Frank Hanses, Arno Mohr, Bernd Salzberger, Matthaus Zerdzitzki, Michael Hilker, Leopold Rupprecht, Dirk Hellwig, Christof Schmid, Lars Siegfried Maier, Can Martin Sag
Summary: This study assessed the diagnostic value of FDG PET/CT in surgically managed IE patients. Results showed that combining FDG PET/CT with Duke criteria can increase the diagnostic sensitivity in PVE patients, with patients having false-negative scans observed to have higher prosthesis age.
JOURNAL OF NUCLEAR CARDIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Yoichi Imori, Ken Kato, Victoria L. Cammann, Konrad A. Szawan, Manfred Wischnewsky, Sara Dreiding, Michael Wuerdinger, Maximilian Schoenberger, Vanya Petkova, David Niederseer, Rena A. Levinson, Davide Di Vece, Sebastiano Gili, Burkhardt Seifert, Masaki Wakita, Noriko Suzuki, Rodolfo Citro, Eduardo Bossone, Susanne Heiner, Maike Knorr, Thomas Jansen, Thomas Muenzel, Fabrizio D'Ascenzo, Jennifer Franke, Ioana Sorici-Barb, Hugo A. Katus, Annahita Sarcon, Jerold Shinbane, L. Christian Napp, Johann Bauersachs, Milosz Jaguszewski, Reiko Shiomura, Shunichi Nakamura, Hitoshi Takano, Michel Noutsias, Christof Burgdorf, Iwao Ishibashi, Toshiharu Himi, Wolfgang Koenig, Heribert Schunkert, Holger Thiele, Behrouz Kherad, Carsten Tschope, Burkert M. Pieske, Lawrence Rajan, Guido Michels, Roman Pfister, Shingo Mizuno, Alessandro Cuneo, Claudius Jacobshagen, Gerd Hasenfuss, Mahir Karakas, Hiroki Mochizuki, Alexander Pott, Wolfgang Rottbauer, Samir M. Said, Ruediger C. Braun-Dullaeus, Adrian Banning, Toshiaki Isogai, Akihisa Kimura, Florim Cuculi, Richard Kobza, Thomas A. Fischer, Tuija Vasankari, K. E. Juhani Airaksinen, Yasuhiro Tomita, Monika Budnik, Grzegorz Opolski, Rafal Dworakowski, Philip MacCarthy, Christoph Kaiser, Stefan Osswald, Leonarda Galiuto, Filippo Crea, Wolfgang Dichtl, Tsutomu Murakami, Yuji Ikari, Klaus Empen, Daniel Beug, Stephan B. Felix, Clement Delmas, Olivier Lairez, Tetsuo Yamaguchi, Ibrahim El-Battrawy, Ibrahim Akin, Martin Borggrefe, John D. Horowitz, Martin Kozel, Petr Tousek, Petr Widimsky, Ekaterina Gilyarova, Alexandra Shilova, Mikhail Gilyarov, Michael Neuhaus, Philippe Meyer, Jose David Arroja, Christina Chan, Paul Bridgman, Jan Galuszka, Gregor Poglajen, Pedro Carrilho-Ferreira, Fausto J. Pinto, Christian Hauck, Lars S. Maier, Kan Liu, Carlo Di Mario, Carla Paolini, Claudio Bilato, Matteo Bianco, Lucas Joerg, Hans Rickli, David E. Winchester, Christian Ukena, Michael Boehm, Jeroen J. Bax, Abhiram Prasad, Charanjit S. Rihal, Shigeru Saito, Yoshio Kobayashi, Thomas F. Luscher, Frank Ruschitzka, Wataru Shimizu, Jelena R. Ghadri, Christian Templin
Summary: This study compared clinical characteristics and in-hospital outcomes between Japanese and European takotsubo syndrome (TTS) patients, revealing differences in triggers and outcomes between the two ethnic groups. Despite worse in-hospital outcomes for Japanese patients, ethnicity itself does not appear to impact TTS patient outcomes. Machine learning analysis identified physical stressors as the most important prognostic factor for both Japanese and European TTS patients.
CLINICAL RESEARCH IN CARDIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael Arzt, Marzena A. Drzymalski, Sarah Ripfel, Sebastian Meindl, Alexander Biedermann, Melanie Durczok, Karoline Keller, Julian Mustroph, Sylvia Katz, Maria Tafelmeier, Simon Lebek, Bernhard Floerchinger, Daniele Camboni, Sigrid Wittmann, Johannes Backs, Christof Schmid, Lars S. Maier, Stefan Wagner
Summary: Patients with sleep-disordered breathing (SDB) have increased oxidized and activated CaMKII levels and CaMKII-dependent Ca spark frequency in the atrial myocardium, independent of major clinical confounders.
Article
Clinical Neurology
Stefan Buchner, Michael Wester, Sarah Hobelsberger, Christoph Fisser, Kurt Debl, Andrea Hetzenecker, Okka W. Hamer, Florian Zeman, Lars S. Maier, Michael Arzt
Summary: Sleep-disordered breathing, particularly obstructive sleep apnea, may impair diastolic function in patients with preserved left ventricular ejection fraction after acute myocardial infarction.
Letter
Critical Care Medicine
Simon Lebek, Philipp Hegner, Rosa Hultsch, Jonas Rohde, Leopold Rupprecht, Christof Schmid, Samuel Sossalla, Lars Siegfried Maier, Michael Arzt, Stefan Wagner
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sophie Moulin, Amandine Thomas, Stefan Wagner, Michael Arzt, Herve Dubouchaud, Frederic Lamarche, Sophie Bouyon, Guillaume Vial, Diane Godin-Ribuot, Jean-Louis Pepin, Claire Arnaud, Elise Belaidi
Summary: This study found that IH-induced ER stress, MAM alterations, and mitochondrial dysfunction were mediated by HIF-1, ultimately leading to cardiomyocyte apoptosis. This suggests that modulation of HIF-1 may help mitigate the adverse effects of sleep-disordered breathing on the heart.
Article
Biochemistry & Molecular Biology
Philipp Hegner, Simon Lebek, Benedikt Schaner, Florian Ofner, Mathias Gugg, Lars Siegfried Maier, Michael Arzt, Stefan Wagner
Summary: Left ventricular contractile dysfunction and arrhythmias frequently occur in patients with sleep-disordered breathing. This study examined a mouse model of obstructive sleep apnea (OSA) and found that inhibition of the oxidative activation of CaMKII provided cardioprotection, suggesting therapeutic implications. The study demonstrated the association between OSA and contractile dysfunction as well as pro-arrhythmic activity, and shed light on the underlying mechanisms.
Article
Cardiac & Cardiovascular Systems
Matthias Scholz, Thomas Meyer, Lars S. Maier, Karl Heinrich Scholz
Summary: This study investigated the relationship between chronic total occlusion (CTO) in a non-infarct-related artery (NIRA) and adverse outcomes in ST-segment-elevation myocardial infarction (STEMI). The presence of CTO posed a threat to coronary blood supply, and the origin of intercoronary collaterals was associated with patient survival rate. The results showed that CTO collateralization from the IRA distal to the acute occlusion was associated with a better prognosis.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Cell Biology
Laura von Schledorn, David Puertollano Martin, Nicole Cleve, Janina Zoellner, Doris Roth, Ben Ole Staar, Jan Hegermann, Felix C. Ringshausen, Janna Nawroth, Ulrich Martin, Ruth Olmer
Summary: Primary ciliary dyskinesia (PCD) is a rare genetic disorder that affects the function of motile cilia in the respiratory tract, leading to chronic inflammation and lung damage. Current treatment options for PCD are only symptomatic, highlighting the need for curative therapies. In this study, researchers developed an in vitro model using human induced pluripotent stem cells (hiPSC) to recapitulate the diseased phenotype of PCD at the molecular, structural, and functional levels.
Letter
Cardiac & Cardiovascular Systems
Julian Mustroph, Maria J. J. Baier, Denise Unsin, Zdenek Provaznik, Kostiantyn Kozakov, Simon Lebek, Daniel Tarnowski, Soenke Schildt, Niels Voigt, Stefan Wagner, Lars S. S. Maier, Stefan Neef
Article
Cell Biology
Ian O. Shum, Sylvia Merkert, Svitlana Malysheva, Kirsten Jahn, Nico Lachmann, Murielle Verboom, Helge Frieling, Michael Hallensleben, Ulrich Martin
Summary: Abnormalities during trophoblast development can lead to pregnancy-related complications, but the underlying mechanisms are established during the first trimester. Human pluripotent stem cell (hPSC) technology provides a renewable source of cells for studying these diseases. By optimizing the treatment protocol, we were able to generate trophoblast cells enriched for HLA-G(pos) EVT-like cells, which will aid in understanding placenta-related disorders and investigation of immune tolerance towards the fetus.
Article
Biochemistry & Molecular Biology
Maria Tafelmeier, Verka-Georgieva Blagoeva, Maximilian Trum, Philipp Hegner, Bernhard Floerchinger, Daniele Camboni, Marcus Creutzenberg, Florian Zeman, Christof Schmid, Lars Siegfried Maier, Stefan Wagner, Dominik Linz, Mathias Baumert, Michael Arzt
Summary: The study aimed to evaluate the association between common diseases and nocturnal hypoxemic burden in patients undergoing coronary artery bypass grafting surgery. The results showed that sleep-disordered breathing, obesity, and mild-to-moderate chronic obstructive pulmonary disease were significant predictors of increased nocturnal hypoxemic burden.
Review
Biochemistry & Molecular Biology
Michael Wester, Michael Arzt, Frederick Sinha, Lars Siegfried Maier, Simon Lebek
Summary: Heart failure with preserved ejection fraction (HFpEF) is a widespread disease with high morbidity and mortality, and sleep-disordered breathing (SDB) has been found to be closely associated with the development and progression of the obese HFpEF phenotype. Individualized therapeutic strategies and novel technologies like single-cell transcriptomics or CRISPR-Cas9 gene editing are needed to improve treatment outcomes.
Article
Medicine, General & Internal
Andrej Preveden, Miodrag Golubovic, Marija Bjelobrk, Tatjana Miljkovic, Aleksandra Ilic, Snezana Stojsic, Dragan Gajic, Mila Glavaski, Lars S. Maier, Nduka Okwose, Dejana Popovic, Fausto Barlocco, Arsen Ristic, Guy A. MacGowan, Iacopo Olivotto, Nenad Filipovic, Djordje G. Jakovljevic, Lazar Velicki
Summary: This study revealed that female HCM patients tend to be older, have more severe symptoms, and show a more pronounced clinical presentation, despite being a minority in the patient population. Additionally, there are differences in echocardiographic findings and levels of N-terminal pro-brain natriuretic peptide between genders in HCM patients.
MEDICINA-LITHUANIA
(2022)