Article
Soil Science
Kristyn B. Numa, Jasmine M. Robinson, Vickery L. Arcus, Louis A. Schipper
Summary: The decomposition of labile and stable carbon pools in soil did not vary significantly between different soil types, suggesting that temperature responses might be independent of soil properties. The overall response of soil respiration to temperature is governed by substrate availability, with biological processes dominating when substrates are abundant and physiochemical processes dominating when substrates are limited.
Article
Infectious Diseases
Jatapat Hemapanpairoa, Dhitiwat Changpradub, Wichai Santimaleeworagun
Summary: This retrospective study evaluated the treatment outcomes and factors associated with mortality in patients with ampicillin-susceptible enterococci bloodstream infections. The study found that the 30-day mortality rate was higher in the vancomycin treatment group, and independent factors associated with 30-day mortality included the Pitt bacteremia score, age-adjusted Charlson Comorbidity Index, and vancomycin treatment.
Article
Mathematics
Irene Marinas-Collado, M. Jesus Rivas-Lopez, Juan M. Rodriguez-Diaz, M. Teresa Santos-Martin
Summary: This study calculated the statistically optimal plan for Accelerated Failure Time (AFT) models based on Arrhenius or Eyring equations, and proposed a new three-point mixture design which showed higher D-efficiency compared to other designs.
Article
Multidisciplinary Sciences
Hsiao-Ching Yang, Yung-Chi Ge, Kuan-Hsuan Su, Chia-Cheng Chang, King-Chuen Lin, Vincenzo Aquilanti, Toshio Kasai
Summary: This study investigates the role of water in cellular respiration and its correlation with temperature. Through experiments and modeling, the effects of temperature on enzymes like Phosphofructokinase (PFK-1) in the respiration process are examined. The research reveals the water channel network structure of the PFK-1 tetramer and how temperature regulates respiration rates.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Pauline Campan, Anatja Samouelian, Marc Voltz
Summary: This study evaluated the effectiveness of using the Arrhenius equation to correct temperature effects on pesticide degradation, particularly in high-temperature tropical conditions. The researchers compared different models and found that there was no difference in the temperature dependence of degradation between tropical and temperate soils. They also discovered that the classical Arrhenius equation was only valid for temperatures ranging from 5 to 20 degrees Celsius, suggesting the need for temperature correction adaptations in tropical environments.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
D. Sobotka, J. Zhai, J. Makinia
Summary: This study investigated the temperature dependence of anammox process kinetics, proposing a new generalized temperature model (GTE) to enhance predictions of SAA. The GTE showed strong correlation between measured and predicted data at various temperature ranges, improving understanding of anammox processes at different temperatures.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Multidisciplinary
Mengyi Yang, Ruirui Sun, Pujuan Deng, Yuzhuo Yang, Wenjuan Wang, Jun-Jie Gogo Liu, Chunlai Chen
Summary: Research has revealed the unique searching mechanism of Cas9 under physiological salt conditions, involving both three-dimensional and one-dimensional diffusion, as well as the presence of apparent asymmetric target search regions flanking PAM sites.
Article
Biology
Alejandro Mata-Cabana, Francisco Javier Romero-Exposito, Mirjam Geibel, Francine Amaral Piubeli, Martha Merrow, Maria Olmedo
Summary: This study investigated the impact of different rate-limiting interventions on the duration of each developmental stage in Caenorhabditis elegans. The results showed that each stage responded differently to environmental perturbations, suggesting stage-specific events play a role in determining the duration. Additionally, the study revealed novel features of temperature dependence in postembryonic development of C. elegans.
Article
Plant Sciences
Bridget K. Murphy, Joseph R. Stinziano
Summary: Understanding biological temperature responses is crucial for predicting global carbon fluxes. An error in the derivation of the modified Arrhenius equation has led to a significant impact on activation energy parameters and a meaningful deviation in whole plant carbon balance. Correcting this error is recommended for future modeling efforts.
Review
Cell Biology
Saumya Yadav, Ambarish Kunwar
Summary: Researchers have investigated the transport properties and temperature-dependent biophysical properties of molecular motor proteins through single-molecule experiments. They have also studied the biochemical properties of these motors to understand their implications on enzymatic activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Alessandro Chiarotto, Bart W. Koes
Summary: A 37-year-old man with a history of low back pain after vigorous sports activities presents with a month-long episode of worsening pain and stiffness in the lower back, particularly in the mornings. Physical examination reveals limited range of motion on lumbar forward bending and tenderness on palpation of the lower back, but no neurologic deficits.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Chemistry, Physical
Vinaya Kumar Golla, Jigneshkumar Dahyabhai Prajapati, Ulrich Kleinekathoefer
Summary: A numerically efficient hybrid Brownian and molecular dynamics approach has been developed and evaluated for studying the translocation of antibiotics through the outer membrane of Gram-negative bacteria. Results suggest that this approach accurately reproduces experimental and simulation findings, making it a promising tool for screening new antibiotics.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2021)
Article
Engineering, Chemical
Ibukun S. Oyelakin, Dorcas Sibanda, Precious Sibanda
Summary: This study discusses the impact of the nonlinear relationship between nonlinear density-temperature and density-concentration on fluid properties in nanofluid flow, revealing that nonlinear density-temperature leads to increased fluid velocity while nonlinear density-concentration reduces fluid speed.
Article
Energy & Fuels
Hussein Al-Hazmi, Xi Lu, Dominika Grubba, Joanna Majtacz, Przemyslaw Kowal, Jacek Makinia
Summary: The study found that temperature has a higher dependency on anammox growth rates at lower temperatures (11-17 degrees Celsius) for biomass cultured at high temperatures. Utilizing intermittent aeration with prolonged non-aerated phases and reduced aeration frequency can help maintain high and stable deammonification performance at decreasing temperatures.Increasing the non-aerated phases and reducing the frequency of aeration changes also lead to energy savings, sparking more interest in this method.
Article
Biochemistry & Molecular Biology
Joseph Crapse, Nishant Pappireddi, Meera Gupta, Stanislav Y. Shvartsman, Eric Wieschaus, Martin Wuhr
Summary: The Arrhenius equation is effective in predicting the temperature dependence of complex multi-step biological processes, with significant deviations observed at low and high temperatures.
MOLECULAR SYSTEMS BIOLOGY
(2021)
Article
Chemistry, Medicinal
Jigneshkumar Dahyabhai Prajapati, Crystal Mele, Mehmet Alphan Aksoyoglu, Mathias Winterhalter, Ulrich Kleinekathoefer
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2020)
Article
Biology
Julia Vergalli, Alessio Atzori, Jelena Pajovic, Estelle Dumont, Giuliano Malloci, Muriel Masi, Attilio Vittorio Vargiu, Mathias Winterhalter, Matthieu Refregiers, Paolo Ruggerone, Jean-Marie Pages
COMMUNICATIONS BIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Sushil Pangeni, Jigneshkumar Dahyabhai Prajapati, Jayesh Bafna, Mohamed Nilam, Werner M. Nau, Ulrich Kleinekathoefer, Mathias Winterhalter
Summary: This study focused on quantifying the passage of the large peptide protamine (Ptm) across CymA, a passive channel for cyclodextrin uptake. Results showed that the kinetics of Ptm entry through CymA was independent of its concentration, with about 1-2 events per second and long dwell times observed per channel. Additionally, voltage-dependent entry of single Ptm peptide molecules into the channel was resolved, with extrapolation to zero voltage revealing a translocation rate of about one molecule per second per channel.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Multidisciplinary
Jigneshkumar Dahyabhai Prajapati, Ulrich Kleinekathoefer, Mathias Winterhalter
Summary: Traditional antibiotics are facing the challenge of Gram-negative bacteria developing resistance, prompting the urgent need for new antibacterial agents. Designing new antibiotic molecules that can rapidly penetrate Gram-negative bacteria and achieve lethal intracellular drug accumulation is a major challenge. The lack of simple experimental techniques to measure antibiotic uptake and local concentrations in subcellular compartments is a limiting factor.
Article
Materials Science, Biomaterials
Petar O. Nikiforov, Beata Hejja, Richard Chahwan, Christian Soeller, Fabrice Gielen, Catalin Chimerel
Summary: The development of a new functional phenotype flow cytometer (FPFC) allows cells to be separated into different subpopulations based on their real-time responses to stimuli, providing a new method for studying cellular heterogeneity. The FPFC design enables rapid analysis and sorting of hundreds of cells. The effectiveness of the FPFC design and operation was validated through sorting cancer cells based on their response to a specific stimulus.
Article
Multidisciplinary Sciences
Samira Sadeghi, Siddharth Deshpande, Girish Vallerinteavide Mavelli, Alphan Aksoyoglu, Jayesh Bafna, Mathias Winterhalter, R. Manjunatha Kini, David P. Lane, Chester L. Drum
Summary: In vitro protein folding often leads to aggregation and low yields. Researchers have used nanoscale exoshells to enhance soluble yield, functional yield, and specific activity of folded proteins.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Tanzir Ahmed, Jayesh Arun Bafna, Roland Hemmler, Karsten Gall, Richard Wagner, Mathias Winterhalter, Michael J. Vellekoop, Sander van den Driesche
Summary: This work presents a microsystem setup for sensitive biological membrane translocation measurements. Thin free-standing synthetic bilayer lipid membranes (BLM) are constructed in microfabricated silicon nitride apertures and used for electrophysiological measurements of pore proteins. The integration of pore-forming proteins is achieved through direct reconstitution or reconstitution via outer membrane vesicles (OMVs) released from Gram-negative bacteria. This microsystem allows for investigation of fast gating events, pore blocking, and gating events of small pores.
Article
Chemistry, Multidisciplinary
Jiajun Wang, Jigneshkumar Dahyabhai Prajapati, Fan Gao, Yi-Lun Ying, Ulrich Kleinekathoefer, Mathias Winterhalter, Yi-Tao Long
Summary: Chirality is important but often overlooked in biological and chemical processes due to technical limitations. Researchers have used an electrostatically asymmetric membrane channel to successfully identify chiral amino acids in single peptides. This study provides a new approach for developing chiral recognition and protein sequencing methods.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Food Science & Technology
Skye Marshall, Beth McGill, Helen Morcrette, C. Peter Winlove, Catalin Chimerel, Peter G. Petrov, Monika Bokori-Brown
Summary: This study investigates the activity of Clostridium perfringens epsilon toxin (Etx) in different cell lines and lipid environments. The results show that the efficiency of Etx binding to cells is species-specific and influenced by surface-exposed tyrosine residues, while the pore-forming ability of Etx is affected by both the cell and lipid environment.
Article
Biology
Muriel Masi, Julia Vergalli, Ishan Ghai, Andrea Barba-Bon, Therese Schembri, Werner M. Nau, Daniel Lafitte, Mathias Winterhalter, Jean-Marie Pages
Summary: This study investigated the translocation and killing effects of different cephalosporins on Gram-negative bacteria. The results revealed that the activity and efficiency of the drugs were influenced by the expression of specific porins. Through experimentation and analysis, it was found that the net negative charge of one of the cephalosporins contributed to its higher permeation through less cation-selective porins. These findings provide insights into the susceptibility of specific bacteria strains to cephalosporins.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Igor V. Bodrenko, Tsedenia Alemu Zewdie, Jiajun Wang, Eshita Paul, Susanne Witt, Mathias Winterhalter
Summary: This study investigates protein-protein interaction using electrophysiology, focusing on the structure of the TolC-AcrA-AcrB complex and the inhibitory effect of potential efflux pump inhibitors. The findings suggest that the fluctuation of ion current and the average current through the TolC channel can be used as complementary measures to assess the interaction of the complex.
Article
Chemistry, Medicinal
Abhishek Acharya, Ishan Ghai, Claudio Piselli, Jigneshkumar Dahyabhai Prajapati, Roland Benz, Mathias Winterhalter, Ulrich Kleinekathoefer
Summary: In this study, the molecular mechanism of an antibiotic permeating through a bacterial channel is revealed, with a focus on the role of conformational dynamics of the constriction loop. The dynamics of the L3 loop, specifically the F118 to S125 segment, at the constriction regions of the OmpF porin is investigated using temperature accelerated sliced sampling approach. The importance of hydrogen-bond stabilization in the conformational dynamics of the L3 loop is demonstrated through single channel electrophysiology experiments and applied-field simulations. The findings suggest that conformational dynamics of the constriction loop may play a general role in antibiotic permeation through porins.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Chemistry, Multidisciplinary
Yao Chen, Andrea Barba-Bon, Bohumir Gruner, Mathias Winterhalter, M. Alphan Aksoyoglu, Sushil Pangeni, Maryam Ashjari, Klaudia Brix, Giulia Salluce, Yeray Folgar-Camean, Javier Montenegro, Werner M. Nau
Summary: Cobalt bisdicarbollides (COSANs) are used as selective and efficient molecular carriers to transport hydrophilic oligopeptides through membranes. They can permeate lipid bilayer membranes without causing membrane damage. COSANs transport arginine-rich and lysine-rich peptides effectively, but cannot transport small molecules or anionic cargos. The transportation mechanism is a molecular carrier mechanism, and living cell experiments show that a fluorescent peptide cargo can be delivered into the cytosol.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Physical
Stefan Milenkovic, Jiajun Wang, Silvia Acosta-Gutierrez, Mathias Winterhalter, Matteo Ceccarelli, Igor V. Bodrenko
Summary: Transmembrane beta-barrel proteins are versatile candidates for various technological applications and their behavior is influenced by environmental conditions. Comparing two highly homologous porins, OmpF and OmpC, we observed subtle amino acid substitutions that can modulate mass transport properties. These differences can be attributed to the disparities in the environmental conditions under which the porins are expressed. Our analysis not only highlights the advantages of enhanced sampling methods, but also provides valuable insights into the biological function and technical applications of these proteins.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Jiajun Wang, Jigneshkumar Dahyabhai Prajapati, Ulrich Kleinekathoefer, Mathias Winterhalter
