Review
Physics, Applied
Maurits R. R. de Planque
Summary: The ion flow through channel proteins embedded in a lipid bilayer membrane can be recorded as an electrical current, enabling biophysical characterization and pharmacological drug screening at a single-channel level. This concise review introduces the bilayer recording methodology, with an emphasis on the requirements for full electrophysiology assays. The self-assembled lipid bilayer, formed in a similar to 100 mu m diameter aperture in between two aqueous chambers, is critical. Various approaches to increase the measurement throughput by scaling to aperture arrays are discussed in terms of current-amplifier technology, bilayer stability, ion channel incorporation, system functionality and obtained single-channel data. The various bilayer recording platforms all have advantages and limitations. Combining the strengths of the different platform architectures, for example, the use of shaped apertures, will be essential to realize and also automate parallel ion channel recordings.
JAPANESE JOURNAL OF APPLIED PHYSICS
(2022)
Article
Chemistry, Analytical
Ryusuke Miyata, Daisuke Tadaki, Daichi Yamaura, Shun Araki, Madoka Sato, Maki Komiya, Teng Ma, Hideaki Yamamoto, Michio Niwano, Ayumi Hirano-Iwata
Summary: A microarray of stable solvent-free bilayer lipid membranes (BLMs) formed in microfabricated silicon chips was reported to improve the efficiency of ion-channel protein reconstitution system for functional analysis and drug screening. It successfully demonstrated parallel recordings of ion-channel activities from multiple BLMs using the hERG potassium channel, which is associated with arrhythmic side effects following drug treatment.
Article
Biochemistry & Molecular Biology
Mohammed A. Abosheasha, Toru Itagaki, Yoshihiro Ito, Motoki Ueda
Summary: The LxxxLxxxL sequence is crucial for nanotube self-assembly, as evidenced by the formation of nanotubes with different diameters and the fabrication of double-layer nanotubes by combining two types of nanotubes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Andrea Mosseri, Maria Sancho-Albero, Marilisa Leone, Donatella Nava, Francesco Secundo, Daniela Maggioni, Luisa De Cola, Alessandra Romanelli
Summary: This study reports the formation of homogeneous chiral fibers through self-assembly of peptide nucleic acids. Nucleobases play a key role in determining the morphology and chirality of the fibers. By spectroscopic characterization and microscopy studies, a structural model consistent with the experimental evidence was obtained.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Tyler D. Jorgenson, Hadi M. Zareie, Mehmet Sarikaya, Rene M. Overney
Summary: The spontaneous co-organization of distinct biomolecules at interfaces enables many of Nature's hierarchical organizations. Binary peptide assembly at graphite surfaces can fabricate biomolecular nano-mosaics with predictable structures. The ability to tune such structures facilitates the bottom up fabrication of high-density, multifunctional interfaces for nanotechnology.
Article
Chemistry, Multidisciplinary
Yusuke Shimizu, Kohei Sato, Kazushi Kinbara
Summary: Inspired by natural calcium-induced reversible assembly and disassembly of membrane proteins, a phosphorylated amphiphile (PA) containing an oligo(phenylene-ethynylene) unit as a hydrophobic unit and a phosphate ester group as a hydrophilic calcium-binding unit was developed. The study demonstrated that PA can reversibly assemble and disassemble within lipid bilayer membranes in response to the sequential addition of calcium chloride and ethylene-diaminetetraacetic acid as a synthetic molecule for the first time.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Yurong Zhao, Hao Qi, Limin Zhang, Chunyong He, Feng Wei, Dong Wang, Jie Li, Kai Qi, Xuzhi Hu, Jiqian Wang, Yubin Ke, Chunqiu Zhang, Jian R. Lu, Hai Xu
Summary: This study investigates the morphology transition of peptide self-assembly and finds that shifting the position of the terminal residue can achieve the transition from nanofibers to nanotubes. The underlying mechanism is revealed, and it is found that the formation of giant nanotubes with a bilayer shell structure is promoted by inter-molecular C-terminal H-bonding. This morphological transformation method is applicable to other analogues and provides a convenient approach for predicting peptide nanostructures.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Physics, Fluids & Plasmas
Mingyuan Ma, Christoph A. Haselwandter
Summary: The study presents a methodology for the self-assembly of lipids and Piezo proteins into polyhedral bilayer vesicles, validated with bacterial mechanosensitive channels. The radius of curvature of the Piezo dome is found to be a critical control parameter for the self-assembly of Piezo vesicles, with various symmetries observed as the radius increases.
Article
Engineering, Chemical
Keita Yano, Masayuki Iwamoto, Takaaki Koshiji, Shigetoshi Oiki
Summary: This study establishes an accurate method for evaluating the osmotic permeability of lipid bilayers under constant tension, and known unstirred layers. This method helps to better understand the water flux process across cell membranes by quantifying membrane tension and unstirred layers.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Amber R. Titus, Ellyse N. Ridgway, Rebecca Douglas, Elena Sanchez Brenes, Elizabeth K. Mann, Edgar E. Kooijman
Summary: Lipid droplets are intracellular organelles with a lipid monolayer, and the mechanism by which cytosolic LD binding proteins recognize and bind to this lipid interface remains poorly understood. The amphipathic alpha-helix bundles shared between cytosolic LD binding proteins and apolipoproteins play a key role in lipid binding. The recruitment and insertion of perilipin 3 at mixed lipid monolayers show differences between the full-length protein and its C-terminal alpha-helix bundle, highlighting distinct lipid binding roles for different domains of the protein.
Article
Biochemistry & Molecular Biology
Melvin Wei Shern Goh, Yuzuru Tozawa, Ryugo Tero
Summary: Artificial lipid bilayer systems are valuable for studying ion channels, and in this study, the assembly and structure of the hERG channel prepared in a cell-free synthesis system were investigated using AFM.
Article
Polymer Science
Thomas Babut, Mona Semsarilar, Marc Rolland, Damien Quemener
Summary: In this study, a waterborne approach was proposed to co-assemble peptide amphiphiles (PA) and polyelectrolytes into porous membranes with nanostructured networks. Various production methods were explored to shape the material and optimize the structure, leading to a membrane material in the ultrafiltration range with a pore radius of about 7.6 nm.
Article
Chemistry, Multidisciplinary
Jin Yan, Yang Wang, Xiao Li, Dongnan Guo, Zhengjun Zhou, Ge Bai, Jianhui Li, Na Huang, Jiajie Diao, Yong Li, Wangxiao He, Wenjia Liu, Kaishan Tao
Summary: Self-assembling peptide hydrogels present a promising approach for treating liver bleeding, with a nano-band-aid (NBA) developed through a three-stage self-assembly process of two functionalized peptides demonstrating rapid and effective control of bleeding in animal experiments.
Article
Chemistry, Physical
Forough Ghasem Zadeh Moslabeh, Farzaneh Fouladgar, Amir Jafari, Neda Habibi
Summary: This study demonstrates the possibility of substrate-free peptide self-assembly through acoustic levitation and investigates the effects of peptide concentration and acoustic wave velocity amplitude on the resultant nano-structures.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Chemistry, Multidisciplinary
Ya Jie Chen, Qi Wen Deng, Li Wang, Xiao Chun Guo, Jian Yuan Yang, Ting Li, Zhengshuang Xu, Hon Cheung Lee, Yong Juan Zhao
Summary: This study constructed a novel nanobody-drug conjugate (NDC) by incorporating the amphipathic peptide GALA, which effectively improved cytotoxicity by one to two orders of magnitude. Mechanistic studies revealed that tethering to lipids induced GALA to form a helix, significantly enhancing endocytosis. This approach provides a general strategy for enhancing the anti-cancer efficacy of protein-drug conjugates and increasing the efficiency of endocytosis-dependent cell delivery of other types.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Benjamin Gerdes, Rebecca M. Rixen, Kristina Kramer, Enrico Forbrig, Peter Hildebrandt, Claudia Steinem
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Yihui Xu, Jan Kuhlmann, Martha Brennich, Karlo Komorowski, Reinhard Jahn, Claudia Steinem, Tim Salditt
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2018)
Article
Biochemistry & Molecular Biology
Damian Pawolski, Christoph Heintze, Ingo Mey, Claudia Steinem, Nils Kroeger
JOURNAL OF STRUCTURAL BIOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Susann Spindler, Jeremias Sibold, Reza Gholami Mahmoodabadi, Claudia Steinem, Vahid Sandoghdar
Review
Biochemistry & Molecular Biology
Markus Schoen, Ingo Mey, Claudia Steinem
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2019)
Article
Biophysics
Raphael Hubrich, Yongsoo Park, Ingo Mey, Reinhard Jahn, Claudia Steinem
BIOPHYSICAL JOURNAL
(2019)
Meeting Abstract
Biophysics
Claudia Steinem
BIOPHYSICAL JOURNAL
(2019)
Article
Multidisciplinary Sciences
Annika Bartsch, Salome Llabres, Florian Pein, Christof Kattner, Markus Schoen, Manuel Diehn, Mikio Tanabe, Axel Munk, Ulrich Zachariae, Claudia Steinem
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Multidisciplinary
Nadine A. Schilling, Anne Berscheid, Johannes Schumacher, Julian S. Saur, Martin C. Konnerth, Sebastian N. Wirtz, Jose M. Beltran-Belena, Alexander Zipperer, Bernhard Krismer, Andreas Peschel, Hubert Kalbacher, Heike Broetz-Oesterhelt, Claudia Steinem, Stephanie Grond
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Multidisciplinary
Jeremias Sibold, Katharina Kettelhoit, Loan Vuong, Fangyuan Liu, Daniel B. Werz, Claudia Steinem
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Multidisciplinary
Jonas Schaefer, Jessica Nehls, Markus Schoen, Ingo Mey, Claudia Steinem
Article
Biophysics
Peter Muehlenbrock, Kira Herwig, Loan Vuong, Ingo Mey, Claudia Steinem
BIOPHYSICAL JOURNAL
(2020)
Article
Multidisciplinary Sciences
Thomas Schubert, Taras Sych, Josef Madl, Maokai Xu, Ramin Omidvar, Lukas J. Patalag, Annika Ries, Katharina Kettelhoit, Annette Brandel, Yves Mely, Claudia Steinem, Daniel B. Werz, Roland Thuenauer, Winfried Roemer
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Physical
Jeremias Sibold, Vera E. Tewaag, Thomas Vagedes, Ingo Mey, Claudia Steinem
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2020)
Article
Chemistry, Physical
Mathias Bosse, Jeremias Sibold, Holger A. Scheidt, Lukas J. Patalag, Katharina Kettelhoit, Annika Ries, Daniel B. Werz, Claudia Steinem, Daniel Huster
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2019)