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Biochemistry & Molecular Biology
Randi Yeager, G. Guy Bushkin, Emily Singer, Rui Fu, Benjamin Cooperman, Michael McMurray
Summary: Researchers found that dormant yeast spores of each mating type contain transcripts representing mating pheromones and receptors, with translation of these components being repressed until germination. Additionally, a rare yeast gene Mata1 was found to be enriched in a spores of mating type a, with its transcripts remaining mostly unspliced.
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Multidisciplinary Sciences
Kyle D. Pedro, Luis M. Agosto, Jared A. Sewell, Kimberly A. Eberenz, Xianbao He, Juan I. Fuxman Bass, Andrew J. Henderson
Summary: Through a high-throughput screen, researchers identified 42 human transcription factors and 85 protein-DNA interactions related to HIV-1 and HIV-2, shedding light on their roles in HIV replication, transcription, and latency. Experimental results confirmed that transcription factors like KLF2, KLF3, and PLAGL1 have different regulatory effects on the transcription of HIV-1 and HIV-2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Genetics & Heredity
Shary N. Shelton, Sarah E. Smith, Jay R. Unruh, Sue L. Jaspersen
Summary: The INM proteome differs between gametes and mitotic cells, with gametes requiring a unique set of INM proteins for gamete formation, synthesized de novo. This suggests changes in the nuclear permeability barrier and inheritance pattern of INM components during gametogenesis.
G3-GENES GENOMES GENETICS
(2021)
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Genetics & Heredity
Shary N. Shelton, Sarah E. Smith, Jay R. Unruh, Sue L. Jaspersen
Summary: By analyzing the differences in the inner nuclear membrane (INM) proteome between mitotic cells and gametes, the study found that gametes contain a distinct INM proteome that needs to be newly synthesized in order to complete gamete formation. Additionally, it was observed that INM components in gametes are generated de novo rather than inherited, showcasing changes in the INM proteome during gametogenesis.
G3-GENES GENOMES GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Most Naoshia Tasnin, Kisara Ito, Haruko Katsuta, Tsuneyuki Takuma, Tasnuva Sharmin, Takashi Ushimaru
Summary: This study reveals the critical role of the PI3KCII-PI3P-Vps27 axis on vacuolar membranes in ESCRT-mediated microautophagy induction and nutrient stress adaptation.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
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Genetics & Heredity
Kunyu Shi, Lele Yang, Xueqing Zhuang, Lan Zhang, Huayu Qi
Summary: The cAMP-dependent protein kinase (PKA) signaling is vital for various processes during mammalian spermatogenesis, including the regulation of gene expression and sperm motility. PKA interacts with numerous proteins specific to testes, such as proteostasis modulators, metabolic enzymes, cytoskeletal regulators, and mitochondrial proteins, indicating its role in regulating protein synthesis and cellular morphogenesis during spermatogenesis. The identification of PKA-RI alpha interacting proteins forms a solid foundation for further investigation into how PKA signaling impacts protein synthesis and cellular morphogenesis in mouse spermatogenesis.
Article
Biochemistry & Molecular Biology
Junwon Kim, Mark D. Rose
Summary: In Saccharomyces cerevisiae, the p21-activated kinase Cla4p regulates polarized morphogenesis and cytokinesis. Cla4p kinase activity is down-regulated during mating, and this is achieved through phosphorylation of Cla4p at multiple sites, including Cla4p-S186, which reduces its kinase activity. Fus3p counteracts Cla4p kinase activity during mating by phosphorylating Fus2p at different sites to promote nuclear export and block import.
Article
Biochemistry & Molecular Biology
Nastaran Farajzadeh, Karen Shahbabian, Yani Bouaziz, Emmanuelle Querido, Pascal Chartrand
Summary: Messenger RNA (mRNA) localization is controlled by She2 RNA-binding protein in budding yeast, and this study reveals that phosphorylation of She2 plays an important role in regulating ASH1 mRNA localization.
Article
Microbiology
David W. Morgens, Divya Nandakumar, Allison L. Didychuk, Kevin J. Yang, Britt A. Glaunsinger
Summary: This study demonstrates the use of CRISPR/Cas9 to rapidly create and screen thousands of mutant viruses in Kaposi's sarcoma-associated herpesvirus (KSHV). By analyzing the expression of viral genes in these mutant viruses, a novel required gene was identified and its specific function was characterized using targeted deep viral sequencing.
Article
Biochemistry & Molecular Biology
Carolin Wevers, Mona Hoehler, Abel R. Alcazar-Roman, Johannes H. Hegemann, Ursula Fleig
Summary: Bacterial pathogens have evolved complex mechanisms to manipulate their hosts and support infection. This study systematically assessed the importance of the microtubule cytoskeleton in Chlamydiae infection. The elimination of microtubules significantly reduced the efficiency of C. pneumoniae infection, indicating the essential role of microtubules in the early stages of infection. A screen in yeast identified multiple chlamydial proteins that modulate microtubule dynamics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Natalie M. Garza, Aaron T. Griffin, Mohammad Zulkifli, Chenxi Qiu, Craig D. Kaplan, Vishal M. Gohil
Summary: This study identified novel genetic regulators of mitochondrial copper homeostasis through a genome-wide screen, including subunits of the AP-3 complex and components of the cellular pH-sensing pathway. These genes impact vacuolar acidity, which in turn perturbs mitochondrial copper homeostasis and CcO function. The study provides insights into how vacuolar pH affects mitochondrial respiration through copper homeostasis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Thomas David Williams, Roberta Cacioppo, Alexander Agrotis, Ailsa Black, Houjiang Zhou, Adrien Rousseau
Summary: This study demonstrates the importance of actin cytoskeleton remodeling for the translation of specific mRNAs under stress conditions. These mRNAs are associated with actin and enriched at cortical actin patches, regulated by the early endocytic protein Ede1.
NATURE CELL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Renjie Wang, Aiwen Huang, Yan Wang, Pengxin Mei, He Zhu, Qianqian Chen, Sankui Xu
Summary: The spatial organization of the nucleus plays a crucial role in genome activities, with carbon source affecting both growth rate and NPC distribution as well as nuclear shape. The nucleolus tends to locate at the nuclear periphery, especially when cells enter quiescence.
STEM CELLS INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Kerstin Dorner, Lukas Badertscher, Bianka Horvath, Reka Hollandi, Csaba Molnar, Tobias Fuhrer, Roger Meier, Marie Sarazova, Jasmin van den Heuvel, Nicola Zamboni, Peter Horvath, Ulrike Kutay
Summary: Ribosome assembly is a crucial process in human cells and is related to congenital diseases and tumorigenesis. This study identified a group of factors involved in 60S subunit assembly in human cells and revealed the connections between 60S subunit maturation and other cellular processes. Additionally, the study discovered a novel function for polyamines in living cells in supporting ribosome synthesis.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Yun Quan, Stephen M. Hinshaw, Pang-Che Wang, Stephen C. Harrison, Huilin Zhou
Summary: Chromosome segregation during cell division is controlled and adapted by kinetochores, which connect spindle microtubules to chromosomal centromeres. A mechanism that regulates kinetochore sumoylation has been identified, with a conserved surface of the Ctf3/CENP-I kinetochore protein providing a binding site for the SUMO chain-removing enzyme Ulp2. Disabling Ulp2 recruitment to kinetochores leads to defective chromosome segregation due to elevated sumoylation levels.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Genetics & Heredity
Liang Jin, Aaron M. Neiman
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Biochemistry & Molecular Biology
Liang Jin, Kai Zhang, Yifeng Xu, Rolf Sternglanz, Aaron M. Neiman
MOLECULAR AND CELLULAR BIOLOGY
(2015)
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Genetics & Heredity
Asaf Ta-Shma, Kai Zhang, Ekaterina Salimova, Alma Zernecke, Daniel Sieiro-Mosti, David Stegner, Milena Furtado, Avraham Shaag, Zeev Perles, Bernhard Nieswandt, Azaria J. J. T. Rein, Nadia Rosenthal, Aaron M. Neiman, Orly Elpeleg
JOURNAL OF MEDICAL GENETICS
(2017)
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Cell Biology
Jae-Sook Park, Mary K. Thorsness, Robert Policastro, Luke L. McGoldrick, Nancy M. Hollingsworth, Peter E. Thorsness, Aaron M. Neiman
MOLECULAR BIOLOGY OF THE CELL
(2016)
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Genetics & Heredity
Tracy L. Callender, Raphaelle Laureau, Lihong Wan, Xiangyu Chen, Rima Sandhu, Saif Laljee, Sai Zhou, Ray T. Suhandynata, Evelyn Prugar, William A. Gaines, YoungHo Kwon, G. Valentin Borner, Alain Nicolas, Aaron M. Neiman, Nancy M. Hollingsworth
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Genetics & Heredity
Reuben Hoffmann, Kariona Grabinska, Ziqiang Guan, William C. Sessa, Aaron M. Neiman
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Biochemistry & Molecular Biology
Leo D. Bemena, Omar Mukama, Aaron M. Neiman, Zijie Li, Xiao-Dong Gao, Hideki Nakanishi
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
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Biochemistry & Molecular Biology
Liang Jin, Kai Zhang, Rolf Sternglanz, Aaron M. Neiman
MOLECULAR AND CELLULAR BIOLOGY
(2017)
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Cell Biology
Tsuyoshi S. Nakamura, Yumi Numajiri, Yuuya Okumura, Junji Hidaka, Takayuki Tanaka, Ichiro Inoue, Yasuyuki Suda, Tetsuo Takahashi, Hideki Nakanishi, Xiao-Dong Gao, Aaron M. Neiman, Hiroyuki Tachikawa
MOLECULAR BIOLOGY OF THE CELL
(2017)
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Genetics & Heredity
Reuben Hoffmann, Kariona Grabinska, Ziqiang Guan, William C. Sessa, Aaron M. Neiman
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Cell Biology
Gregory Omerza, Chong Wai Tio, Timothy Philips, Aviva Diamond, Aaron M. Neiman, Edward Winter
MOLECULAR BIOLOGY OF THE CELL
(2018)
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Multidisciplinary Sciences
Sai Zhou, Rolf Sternglanz, Aaron M. Neiman
Article
Biochemistry & Molecular Biology
Jae-Sook Park, Nancy M. Hollingsworth, Aaron M. Neiman
Summary: The VPS13 family of proteins play a crucial role in intracellular lipid transport and human health, with studies revealing their involvement in various cellular functions including the promotion of ER-phagy. Humans have four different VPS13 orthologs, and amino acid substitution mutations associated with disease help in understanding the multiple functions of VPS13.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Microbiology
Aaron M. Neiman
Article
Microbiology
Kai Zhang, Leor Needleman, Sai Zhou, Aaron M. Neiman